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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume & Issues
Volume 38, Issue 4 - Dec 2008
Volume 38, Issue 3 - Sep 2008
Volume 38, Issue 2 - Jun 2008
Volume 38, Issue 1 - Mar 2008
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Effects of Dandelion (Taraxacum coreanum) Extracts on the Mouse Liver with Acute Toxicated by Mercury Chloride
Cheong, Min-Ju ; Yoon, Jung-Sik ; Huh, Jin ; Roh, Young-Bok ; Choi, Young-Bok ; Kim, Jong-Se ; Lee, Hyun-Hwa ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 1~10
Dandelion has been frequently used as a remedy for women's disease, inflammatory diseases and disorders of the liver and gallbladder. Dandelion extracts water extract, an herbal medication, may have an effect on the activity of hepatic antioxidant enzymes in diabetic rat. This study aims demonstrate the effect of dandelion extracts, one of the natural chelator, on the biochemical and enzyme activity changes in the mouse liver caused by
. Mice approximately 30 gm in weight were grouped into the control, mercury chloride-treated, and the dandelion extracts-treated after mercury chloride groups.
(5 mg/kg) and dandelion extracts (3 g/kg) were delivered orally. Serum AST and ALT were measured, enzyme activity of liver were examined by spectrophotometer and ultrastructural alteration of liver were examined by light and electron microscopy. Dandelion extracts were decreased the increase of serum AST and ALT level induced by mercury. The catalase activity was decreased in the dandelion extracts group. The activity of SOD was dereased, but did not show significant differences. Mercury chloride-treated hepatic cell were irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with dandelion extracts were similar to those of the control group. In conclusion, dandelion extracts may protect the mercury-induced toxicity on Liver.
Effects of Electrolyzed Alkaline Reduced Water on Echinostoma hortense Infection and Immune Response in C57BL/6 Mice
Kim, Dong-Heui ; Deung, Young-Kun ; Jin, Dan ; Huang, Xue Zhu ; Qi, Xu Feng ; Kim, Kwang-Yong ; Lee, Kyu-Jae ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 11~19
To examine the effect of the electrolyzed alkaline reduced water (ERW) on animal immunity, by employing Echinostoma hortense that is a parasite in the small intestine, the immune response of C57BL/6 was examined. To C57BL/6 mice, Echinostoma hortense metacercaria 15 per animal was in oculate dorsally, the worm was collected after 2 weeks, and the change of goblet cells and mast cells in the mucosa of small intestine was examined, and by using a protein chip, the change of cytokines in the serum was compared and observed. As a result, average 8.3 worms were collected from the C57BL/6 mice infected with E. hortense, and in the group fed with the ERW, average 10 worms were collected. In regard to the examination of the change of goblet cells, in the experimental group infected with E. hortense and fed with the ERW, average 4.3 worms per villus were detected, hence, it was found that the expression of goblet cells was low (p<0.001). Regarding the examination of the change of mast cells, similarly, in the group infected with E. hortense and fed with the ERW, average 11 worms per villus were detected, and it appears to be less than control group (p<0.001). Regarding the expression of cytokines in mouse serum, in comparison of the experiment group infected with E. hortense and control group, in the expression of the Th1 cytokines IL-6, IL-
, and IL-2, and the Th2 cytokines IL-4, IL-5, IL-10, and IL-13, a significant difference was not detected. In our study, it was found that in the infection of E. hortense, the ERW mediates its effect on the number of goblet cells and mast cells in the intestinal mucosa, and simultaneously, the worm expulsion was delayed, and thus the conclusion that the ERW mediated its effect on the intestinal immunity of mice was obtained.
Structural Features of the Glandular Trichomes in Leaves of Carnivorous Drosera anglica Huds.
Baek, Kyung-Yeon ; Kim, In-Sun ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 21~28
Carnivorous plants vary in their unique features of morphology, ultrastructure and biochemical properties by species. Furthermore, prey-capturing mechanism as well as structural and physiological adaptations have been used for grouping various carnivorous species. In Drosera plants, glandular trichomes, which develop in the leaf epidermis, are known to play the most important role during the prey capturing process. The present study examined such trichomes, focusing on the glandular type, in leaves of Drosera anglica using scanning and transmission electron microscopy. Three types of rudimentary glandular trichomes were found to develop within the folded leaf primordia and immature leaf during early development. The first type, stalked glandular trichomes (Type I), occurred on the margin and upper epidermis of the leaf. With maturation, the longest glandular trichomes having lengthy stalks, ca.
, developed along the margin, while shorter stalked trichomes, ca. up to
, were found on the inner leaf blade. The shorter ones consisted of a globose head having two layers of secretory cells, parenchyma bell cells and tracheids and a multicellular stalk. The stalks gradually decreased in length in centripetal fashion. The second type, Type II, having ca.
short stalks, also developed along the inner blade. Both types secreted mucilage from the secretory cells which had a thin cell wall and cuticle layer. The sessile six-celled glandular trichomes were the third type, Type III, and were
in length. They were distributed most commonly throughout the upper and lower epidermis, petiole and even on the stalk surfaces of the first two types of trichomes. The third type was also found to be involved in the active secretion. In prey capturing leaves, all trichome types secreted substances through thin cuticles in the head cell wall, which exhibited relatively loose wall components.
Detection Methods of Histochemically-reactive Zinc in the CNS at the Light Microscopical Level
Kim, Yi-Suk ; Kim, Sang-Hyun ; Lee, Beob-Yi ; Lee, Hyun-Sook ; Kim, Sung-Joo ; Jo, Seung-Mook ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 29~34
Small amounts of zinc ions regulate a plentitude of enzymatic proteins, receptors and transcription factors, thus cells need accurate homeostasis of zinc ions. Some neurons have developed mechanisms to accumulate zinc in specific membrane compartment ("vesicular zinc"), which can be evidenced using histochemical techniques. These neurons are the socalled zinc enriched (ZEN) neurons, which accumulate glutamate and zinc inside their synaptic vesicles and release it during synaptic transmission. In the present paper we have studied the distribution of the ZEN terminals in the rat hippo-campus using ZnSe autometallography, Neo-Timm staining, ZnT3 immunohistochemistry and TSQ fluorescence staining.
The Effects of Ultrastructure with Onion (Allium cepa) Skin Extracts on Human Hair Dyeing
Na, Yun-Young ; Cheong, Min-Ju ; Roh, Young-Bok ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 35~41
Onions are commonly available and easily processed, and since their skins are thrown away they could be very useful materials from the viewpoint of environmental preservation. This study aimed to process onion skins into the state of powder and look into the optimum condition for hair dyeing by decoloring virgin hair four times and observing the hair dyeing quality and its mechanical and morphologic changes by the different condition of onion skins in duration, temperature, density, pH, and mordant treatment. The observations of hair surface through scanning microscopy showed the formation of cuticle layer, though in a little blown-up state in the case of the hair dyed with onion skin application, when compared with bleaching hair with the completely dissolved cuticle layer. According to the above findings of experiments, the dyeing quality of the hair dyes with onion skin application was excellent, the dyeing was also feasible without mordants, and the use of Fe as the mordant increased dyeing exhaustion more, which would make it an effective hair dye. These results of the experiments indicate that the natural pigment extracted from onion skins can be actually used for hair dyeing from the viewpoints of the dyeing quality and the skin treatment.
Anticancer Effect of Ascorbic Acid and Saengshik on CT-26 Colon Cancer
Kim, Dong-Heui ; Deung, Young-Kun ; Qi, Xu Feng ; Lee, Young-Mi ; Yoon, Yang-Suk ; Kim, Kwang-Yong ; Chang, Byung-Soo ; Lee, Kyu-Jae ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 43~50
Uncooked powered diet (Saengshik) composed of grains, vegetables, mushrooms and fruits have various physiological functions including strong antioxidant and potent anticancer effects by many kinds of bioactive phytochemicals. The objective of present study was to identify the anticancer effects of vitamin C and saengshik on colon cancer induced by CT-26 cell line in BALB/c mice. As the result, the tumor volumes of vitamin C-mixed diet group (VC) showed no significant differences compared with control group (C) after subcutaneous injection of CT-26 cell lines. However saengshik group (S) showed a significant effect, inhibiting the growth of cancer by 56.2% (
), 48.1% (
), 43.2% (
), 55.5% (
), 69.2% (
) and 70.7% (
) respectively as compared with C group at an interval of 5 days after injection of the CT-26 cells into mice. Also the final tumor volume of S group exerted a significant differences as compared with one of C group (p<0.05). Especially in the case of S group (n=10), the tumors in 2 of 10 mice entirely disappeared at 25th day. Our results suggest that saengshik possess a strong inhibitory action against tumor growth induced by CT-26 colon cancer cell line in the mice. Further studies of saengshik are required to confirm the cancer prevention effect and possibility of adjuvant cancer therapy.
Model Simulation for Assessment of Image Acquisition Errors Affecting Electron Tomography
Jou, Hyeong-Tae ; Lee, Su-Jeong ; Kim, Youn-Joong ; Suk, Bong-Chool ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 51~61
This simulation study examined the effect of data acquisition error including the data type of TEM image, and incident beam intensity of the tilt series on 3D tomograms. Simulation was performed with the 3D head phantom model of Kak and Slaney, and the slightly modified 3D head phantom model with enhanced difference in absorption coefficients. Reconstructed tomogram for the original head phantom model using 8-bit gray-scale image was distorted with extremely high level of noise, while an acceptable result was obtained for the modified model. The results for the original model using wrong formulation for the transmitted beam intensity was proved to be incorrect. The high level of noise along the z direction was found in case of the modified model. On the other hand, the wrong value of incident beam intensity in both models gave distorted results. In order to reconstruct an artifacts-free 3D structure from the projections with invisible features in electron tomography, the 16-bit projection images should be used with the correct incident beam intensity which is applied to Beer's law.
A Review of IWFR Method for HRTEM Image Analysis and Application
Kim, Hwang-Su ;
Applied Microscopy, volume 38, issue 1, 2008, Pages 63~72
The iterative wave-function reconstruction (IWFR) method developed by Allen et al. (2004) was reviewed with concern for its applicability. The high resolution transmission electron microscopy (HRTEM) studies of the materials such as GaAs,
reported in the literature were utilized in this review. In this process the basis of validity, the limiting conditions and the information limit of this method were discussed. It was particularly noted that the phase contrast image of the exit plane wave evaluated from this method reveals not only
-corrected atomic resolution within information limit, but also strong tendency of contrast proportional to the magnitude of the atomic number of compositional atoms in a crystal.