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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 38, Issue 4 - Dec 2008
Volume 38, Issue 3 - Sep 2008
Volume 38, Issue 2 - Jun 2008
Volume 38, Issue 1 - Mar 2008
Selecting the target year
with TRPV1 in the Rat Trigeminal Sensory Nuclei
Moon, Yong-Suk ; Ryoo, Chang-Hyun ; Cho, Yi-Sul ; Kim, Hong-Tae ; Park, Mae-Ja ; Paik, Sang-Kyoo ; Moon, Che-Il ; Kim, Yun-Sook ; Bae, Yong-Chul ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 151~157
Trigeminal primary afferents expressing
or transient receptor potential vanilloid 1 (TRPV1) are involved in the transmission of nociceptive information. In order to characterize
- and TRPV1-immunopositive neurons in the trigeminal ganglion (TG) and trigeminal caudal nucleus (Vc), we performed immunofluorescence experiments using anti-
and anti-TRPV1 antisera and a morphometric analysis. 77.4% (1,401/1.801) of all the
-postive neurons coexpressed TRPV1 and 51.9% (1,401/2,698) of all the THFV1-immunopositive neurons also costained for
in the TG. Immunoreactivity for both
and TRPV1 were present in medium-sized neurons but not in small- and large-sized neurons.
and/or TRPV1-immunopositive fibers were observed in the primary afferents and their associated axons in the Vc. These fibers and terminals were distributed in the superficial lamina of Vc:
-immunopositive fibers and terminals were distributed in the lamina I and II, expecially in the inner part of lamina II (lamina IIi), whereas TRPV1-immunopositive ones were densely detected in the lamina I and outer part of lamina II (lamina IIo). Immunopositive fibers and terminals for both
and TRPV1 were observed on the border between lamina IIi and IIo. These results suggest that terminals coexpressing
and TRPV1 are involved in specific roles in the transmission and processing of orofacial nociceptive information.
Study on the Thermal Analysis of Dyed Hair Depending on the Brightness Level
Kim, Moon-Sun ; Lee, Gui-Young ; Choi, Eun-Young ; Kim, Dong-Heui ; Chang, Byung-Soo ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 159~165
Morphological and physicochemical changes of a woman's virgin hair treated with various dye depths were investigated by using scanning electron microscopy and thermal analyzer. With the hair treated with the dye of the high brightness level, the speed of weight decrease was slower than compared with thermal analysis weight of a normal hair sample. We confirmed that the moisture content and protein composition of hair were changed depending on level depth of coloring agent. Moreover oxidative residues and dye molecules penetrated into the hair cause chemical changes of hair structure. As a result, the heat reaction speed of hair treated with high level coloring agent was made slower than normal hair.
The Effects of Magnesium Rich Sea Mineral Water on Atopic Dermatitis-like Skin Lesions in Hairless Mice
Kim, Dong-Heui ; Lee, Kyu-Jae ; Qi, Xu-Feng ; Lee, Young-Mi ; Yoon, Yang-Suk ; Kim, Jeong-Lye ; Chang, Byung-Soo ; Ryang, Yong-Suk ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 167~174
Atopic dermatitis (AD) is a chronically relapsing inflammatory skin disease that often has asthma and allergic rhinitis. Magnesium salts, the important component of minerals in Dead Sea water, are known to exhibit beneficial effects in inflammatory disease. Favorable effects of magnesium ions and sea water treated to the skin of patients with contact dermatitis have been reported. But histological and immunological investigations are insufficient. This study was performed to examine the inhibitory effect of magnesium-rich sea mineral water on the development of AD-like skin lesions in hairless mice. AD-like skin lesions are induced by the repeated application of 2,4-dinitrochlorobenzene (DNCB). Local application of magnesium-rich sea mineral water on hairless mice skin applied with DNCB inhibited the development of AD-like skin lesions as exemplified by a significant increase in skin hydration (p<0.01), and a decrease in epidermal water loss (p<0.01). Serum IgE level was also significantly decreased (p<0.01). These results suggest that magnesium-rich sea mineral water inhibits the development of DNCB-induced AD-like skin lesions in hairless mice. These observations indicate that magnesium-rich sea mineral water may be alternative and assistant substances for the management of AD.
Structure and Metallothionein Expression during Rat Liver Regeneration Induced by Partial Hepatectomy
Mun, Seung-Hoon ; Jeong, Jin-Joo ; Lee, Yong-Moon ; Shin, Kil-Sang ; Kim, Wan-Jong ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 175~183
Liver regeneration is a result of highly coordinated proliferation of hepatocytes and non-parenchymal liver cells. At this process, induction of metallothionein (MT), which is low molecular and cysteine rich, has been reported. The present study was carried to find the ultrastructure of hepatocytes and determine the expression of MT in regenerating rat liver after partial hepatectomy. As a result, the remnant liver after PH grew fast from 1 day until 7 days. Various changes were morphologically observed. Disintegration of cell plates and liver lobule appeared shortly after PH. And hepatocytes showed the rapid proliferation, characterized by high nuclear cytoplasmic ratio, weak intercellular junctional complexes, chromatin condensation, increase of ribosomes and mitochondria, and temporary increase of lipid droplets. Finally, remodeling of the liver lobule was completed through the rearrangement of blood vessels and cell plates by 7 days after PH. On histochemistry, immunoreactivity indicating the presence of MT appeared moderately throughout the cytoplasm of control rat hepatocyte. After PH, positive reactions for MT increased at the cytoplasm and the nucleus. These results suggest that the remnant liver cells immediately entered cell proliferation and increase of MT expression after PH. It is thought that MT protein might be associated with transfer of some factors needed to cell division from the cytoplasm to the nucleus for regeneration of the liver after PH.
Imaging Plate Technique for the Electron Diffraction Study of a Radiation-sensitive Material under Electron Beam
Kim, Young-Min ; Kim, Yang-Soo ; Kim, Jin-Gyu ; Lee, Jeong-Yong ; Kim, Youn-Joong ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 185~193
An experimental comparison of the detection properties between imaging plate and film for recording the electron diffraction pattern was carried out on a radiation-sensitive material, an aluminum trihydroxide(gibbsite,
), through the electron beam irradiation. Because the imaging plate has a wide dynamic range sufficient for recording extremely low- and high-electron intensities, the range of spatial frequency for the diffraction pattern acquired by the imaging plate was extended to two times larger than the range by the film, especially at a low electron dose condition(
). It is also demonstrated that the imaging plate showed better resolving power for discriminating fine intensity levels even in saturated transmitted beam. Hence, in the respect of investigating the structures of radiation-sensitive materials and cryo-biological specimens, our experimental demonstrations suggest that the imaging plate technique may be a good choice for those studies, which have to use an extremely low electron intensity for recording.
The Effect of Lithospermum erythrorhizon Extracts in UVB-Irradiated Mouse Skin
Song, Seon-Young ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 195~204
This study was intended to identify the effectiveness of Lithospermum erythrorhizon in the UVB-irradiated mouse skin. The C57BL mice were divided into three groups; the control group, the UVB irradiated group(UVB group), and the group treated with Lithospermum erythrorhizon extracts after UVB irradiation(UVB+Le group). 10 mouses were collected and sacrificed at 24 hrs, 48 hrs, 72 hrs, 120 hrs, and 168 hrs, respectively. In the result, the transepidermal water loss (TEWL) was decreased the UVB+Le group than UVB groups by time. At the 168 hrs group was significantly lower(p<0.05). In the result, the melanin value was decreased in the UVB+Le group than UVB group, but meaningless(p>0.05). In the result of erythema index, the UVB+Le group was meaningfully lower at 24 hrs, 48 hrs, and 72 hrs group than UVB group(p<0.05). In the result of scanning electron micrograph observation, the UVB+Le group was allevited swelling than UVB group at the 24 hrs, formation of the scab at the 48 hrs, regular plate shap at the 72 hrs, new keratin observated at the 120 hrs partially, and fine fiber covered epidermis surface at the 168 hrs. In the result of transmission electron micrograph observation, the UVB+Le group was facilitation of increased lamellar bodies and reformation lamellar bodies than UVB group at the all groups. Almost all the structures were recovered at the 160 hrs group. In conclusion, Lithospermum erythrorhizon extracts may recovery on the UVB-irradiated mouse skin.
Effects of 6-aminonicotinamide on the Testes of Golden Hamster
Lee, Jin-Suk ; Choi, Byoung-Young ; Kim, Dong-Heui ; Jung, Won-Sug ; Cho, Byung-Pil ; Yang, Young-Chul ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 205~212
In this study morphological changes of the testes of golden hamsters treated with 6-aminonicotinamide (6-AN, 10 mg/kg body weight) in every two days were investigated using light and transmission electron microscopes. After the 7th injection of 6-AN, body weights of hamsters were significantly reduced, and the weight of testes were markedly reduced in the group of hamsters after 5th injections. Degeneration of seminiferous epithelium appeared first in the group receiving 5th injections, which were followed by severe degenerations after the 9th injection. In the degenerated seminiferous epithelium, deep vacuolization, and destruction of spermatogenic cells and Sertoli cells were also oberved. Multinuclear giant cells were also observed in the lumen of destructed seminiferous epithelium. But there were no edematous changes in the interstitial tissue, and the Leydig cells were found to be relatively intact. Therefore, these results show that 6-AN trigger severe morphological alteration of the spermatogenic cells and Sertoli cells, however Leydig cells are unaltered by 6-AN.
Effects of Prenatal and Restraint Stress on Astrocytes of Amygdala Complex of Rat: I. Effects on the Astrocytic Cell Body
Lee, Ji-Yong ; Choi, Byoung-Young ; Kim, Dong-Heui ; Jung, Won-Sug ; Cho, Byung-Pil ; Yang, Young-Chul ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 213~219
The plasticity of nervous system is generated not only due to changes in neurons but also due to changes in neuroglial cells. Astrocyte is important for maintaining the normal brain function and controlling the neuronal functions. The amygdala receives an array of important sensory information of danger signals. This information is further transduced and integrated to produce the highly adaptive emotion, fear. In this study, morphometric changes in the cell bodies of astrocytes in the amygdala, induced by prenatal stress and restraint stress were examined. For this purpose. rats were classified into 4 groups; control group (CON), only restraint-stressed (starting on P90 for 3 days) group (CONR), prenatally-stressed group (PNS), and prenatally and restraint (on P90 for 3 days) stressed group (PNSR). Astrocytes were verified with anti-GFAP immunohistochemistry, counter stained with methylene blue/azure II and were examined using the Neurolucida. Results showed that astrocytes in the amygdala of PNS rats had significantly larger cell bodies than did CON rats and this was enhanced further by restraint stress. Thus this data showed that hypertrophy of the astrocytic cell bodies of amygdala complex is induced by prenatal and restraint stress.
Immunoelectron Microscopic Study on the Nitric Oxide Synthase in Rat Salivary Glands
Lee, Young-Hwan ; Ko, Jeong-Sik ; Park, Dae-Kyoon ; Park, Kyung-Ho ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 221~233
Endogenous nitric oxide (NO) has been known to regulate many physiological and pathological processes, especially the glandular secretion and blood flow. However, nitric oxide synthase (NOS) responsible for NO synthesis has not been well studied ultrastructurally in rat salivary gland. The present study was performed to investigate the distribution of nitric Oxide synthase isoforms (endothelial. neuronal, and inducible NOS). Immunoelectron microscopic study, using monoclonal mouse anti-endothelial NOS, anti-neuronal NOS, and anti-inducible NOS, was performed in the salivary gland of rat. Endothelial NOS (eNOS)-positive immunoreactivities were most prominent in the secretory granules of serous cells of the salivary gland of the rat. Immunoreactivities were well concentrated on serous secretory granules in the serous cells. However, weak eNOS-positive immunoreactivity was observed in the mucous secretory granules of the mucous cells. Positive endothelial NOS (eNOS) immunoreactivities were most prominent in the secretory granules of intralobular ducts. Ductal secretory granules and acinar serous secretory granules have a similar pattern of labeling as eNOS suggestings. Neural NOS (nNOS)-positive immunoreactivity was not detected in duct systems or in acinar cells. Inducible NOS (iNOS)-positive immunoreactivity was not seen in acinar and ductal cells. These results reveal the presence of eNOS in the salivary gland of the rat, which may be related with regulation of the glandular secretion and blood flow through the gland.
Cellular Imaging of Gold Nanoparticles Using a Compact Soft X-Ray Microscope
Kwon, Young-Man ; Kim, Han-Kyong ; Kim, Kyong-Woo ; Kim, Sun-Hee ; Yin, Hong-Hua ; Chon, Kwon-Su ; Kang, Sung-Hoon ; Park, Seong-Hoon ; Juhng, Seon-Kwan ; Yoon, Kwon-Ha ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 235~243
A compact soft x-ray microscope operated in the 'water window' wavelength region (
) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.
Vitamin C Enhances the Effect of Etoposide to Inhibit Human Prostate Cancer Growth in vitro
Lee, Myeong-Seon ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 245~250
Etoposide (Eto) is chemotherapeutic compounds that is currently used in the treatment of metastatic prostate cancer but new therapeutic agents are needed for the treatment of androgen-independent prostate cancer. The objective of the present study was to determine whether vitamin C (VC), the antioxidant, plays a role in regulating the growth of prostate cancer cell lines and whether VC has synergistic effect to tumor cell killing by chemotherapeutic drugs. Androgen-dependent LNCaP and androgen-independent DU-145 prostate cancer cell lines were used in this study. Both cells presented increase of dose- and time-dependent cytotoxicity in Eto-treated cultures. The combined treatment with Eto and VC significantly increased the percentage of apoptotic cells compared to Eto-treated cells(p<0.05). The present findings demonstrated that VC inhibited the growth of prostate cancer cell lines by Eto-mediated cytotoxicity and induced apoptosis. These results suggest that the chemotherapeutic effect of Eto on prostate cancer can be enhanced by VC.
Study on the Tensile Strength of Bleached Hair
Lee, Gui-Young ; Chang, Byung-Soo ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 251~257
Human hair experience tensile forces during combing, bleaching and permanent dyeing processes. We have used rheometer to characterize bleached hair tensile response. Also, we investigated to morphological changes after tensile test of virgin hair and bleached hair using scanning electron microscopy. In tensile test, the tensile strength of virgin hair was
. They were measured as
for after the first, the second and the third trials of bleaching were done, respectively. Virgin hair had a higher tensile force than bleached hair. Tensile strength got lowered as more bleaching trials were done. In the observation of scanning electron microscopy, the fracture and lift-off of the cuticle outer layer were shown in virgin hair and bleached hair. The bleached hair were more lifted off at the outer cuticle layer than virgin hair. Consequently, chemical damage of bleaching agent caused weakening of the outer cuticle in the hair.
Microscopic Study of Decomposition-Inhibition in Stabilized
Gas in Kidney of Rat with Passage of Time
Hwang, Kyu-Sung ; Choi, Ki-Ju ; Paik, Doo-Jin ; Lim, Do-Seon ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 259~264
gas has been used for many years by the food industry as a strong oxidizing and sanitizing agent that has broad and high biocidal effectiveness. Therefore, "stabilized
" gas may be used in fields of disinfectant and sterilization. But, there have been few studies on the decomposition-inhibition effect of stabilized
gas with passage of time. The main purpose of this study was to examine the decomposition-inhibition effect of stabilized
gas and the morphological change of kidney by measuring of the light and electron microscope. Sprague-Dawley (SD) rats weighting from 230 gm to 250 gm were used as experimental animals. Under ether anesthesia, the right kidney of rat was obtained. Put each sample in
incubator, we obtained each sample after 0 day, 1 day, 2 days, 3 days, 4 days and 5 days. We proceeded the observation of light and electron microscope. The results obtained in this study reveal that stabilized
gas is an effective decomposition inhibitor until 2 days that was conducted at
Electron Microscopic Studies on the Morphological Differences of Ethnic Hair
Lee, Gui-Young ; Chang, Byung-Soo ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 265~273
We investigated the morphological differences of the ethnic hairs using scanning electron microscopy and transmission electron microscopy, sampled from African, Asian and Caucasian women. Transverse section of African hair had a highly elliptical type whereas those of Asian and Caucasian hairs had circular and elliptical types, respectively. The diameter of African hair showed wide variations from 90 to 115
. The diameter of Asian hair was 100
and Caucasian hair had a lesser diameter of 80
on average. African hair were much more damaged in cuticle layer compared with Asian or Caucasian hairs. In particular, endocuticle of cuticle cell had a lot of holes in it, which resulted that it tends to be easily broken. Phaeomelanin in the cortex of Caucasian hair had a concentrically helical structure in it.
A Glance of Electron Tomography through 4th International Congress on Electron Tomography
Rhyu, Im-Joo ; Park, Seung-Nam ;
Applied Microscopy, volume 38, issue 3, 2008, Pages 275~278
Electron tomography (ET) is an electron microscopic technique for obtaining a 3-D image from any electron microscopy specimen and its application in biomedical science has been increased thanks to development of electron microscopy and related technologies during the last decade. There are few researches on ET in Korea during this period. Although the importance of ET has been recognized recently by many researchers, initial approach to electron tomographic research is not easy for beginners. The 4th International Congress on Electron Tomography (4 ICET) was held on Nov
, 2006, at San Diego. The program dealt instrumentation, reconstruction algorithm, visualization/quantitative analysis and electron tomographic presentation of biological specimen and nano particles. 1 have summarized oral presentations and analyzed the posters presented on the meeting. Cryo-electron microscopic system was popular system for ET and followed conventional transmission electron microscopic system. Cultured cell line and tissue were most popular specimens analyzed and microorganisms including bacteria and virus also constituted important specimens. This analysis provides a current state of art in ET research and a guide line for practical application of ET and further research strategies.