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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 40, Issue 4 - Dec 2010
Volume 40, Issue 3 - Sep 2010
Volume 40, Issue 2 - Jun 2010
Volume 40, Issue 1 - Mar 2010
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Age Determination by Tooth Wear and Histological Analysis of Seasonal Variation of Breeding in the Lesser White-Toothed Shrew, Crocidura suaveolens
Jeong, Soon-Jeong ; Yoon, Myung-Hee ; Kim, Sook-Hyang ; Ham, Joo-Hyun ; Lim, Do-Seon ; Choi, Baik-Dong ; Park, Jin-Ju ; Jeong, Moon-Jin ;
Applied Microscopy, volume 40, issue 3, 2010, Pages 125~132
Captured specimens of the lesser white-toothed shrew, Crocidura suaveolens were classified into three age classes by tooth wear and seasonal variations of reproductive organs were investigated. Molars of juveniles had not tooth wear and the height of the third molars were lower than the first and second molars, young adults had smooth tooth wear and the third molars reached to the first and second molars, and old adults had heavy tooth wear and the third molars also reached to the first and second molars. On the basis of histological examination, seasonal variation of breeding was confirmed that breeding season of adult males was from early February to early October, having a peak of the breeding in April and July, and non-breeding season was from in the middle of October to late January. Young and old adult males of the breeding season had large testes with enlarged seminiferous tubules filling with numerous germ cells and expanded caudal epididymides with a vast number of spermatozoa, Young and old adult males of the non-breeding season had the small testes with the extremely slender seminiferous tubules filling with only spermatogonia and the reduced caudal epididymides without spermatozoa. Males weighing more than 3.9 g in the body weight and 0.013 g in the testis and epididymis weight reached sexual maturation in breeding season, and the females weighing more than 3.8 g in body weight of the breeding season were pregnant condition having 5~6 litters or had the Graafian follicles and the corpus lutea in the ovary.
Cisplatin and Extract of Tissue Cultured Mountain Ginseng-Induced Apoptosis in Human Cervical Cancer Cells
Lee, Myeong-Seon ;
Applied Microscopy, volume 40, issue 3, 2010, Pages 133~138
Mountain ginseng is a perennial crop rarely found in the deep mountains of Korea. The medicinal effect of the mountain ginseng is well known as a panacea in traditional Chinese medicine for a long time. But scientific studies to elucidate the medicinal effect of the mountain ginseng have never been made on account of lack of sample. Recently an improved method of adventitious root culture system through the use of bioreactor has been developed in Panax ginseng that seems to be a reliable way of commercialization of root derived secondary metabolites. This experiment was conducted to evaluated chemotherapeutic effect against human cervical cancer cells by cisplatin (CDDP) and extract of tissue cultured mountain ginseng (ETCMG). CDDP and ETCMG-induced apoptotic cell death in human cervical cancer cell line, HeLa was confirmed by the analysis of cell growth, morphological changes, DNA fragmentation, flow cytometry showed that ETCMG is an inducer of apoptosis and synergizes with CDDP. These results suggest that ETCMG present evidence of anticancer effect and could have a possibly natural therapeutic potential in cervical cancer patients.
A Study of Effectiveness of Lithospermum erythrorhizon Extracts on the Skin by UVB-Irradiation C57BL/6 Mouse
Lee, Hyun-Hwa ; Song, Seon-Young ;
Applied Microscopy, volume 40, issue 3, 2010, Pages 139~145
This study was intended to identify the effectiveness of Lithospermum erythrorhizon in the UVB-damaged mouse skin. The C57BL/6 mouse that weighted about 18 g were divided into three groups; the control group (A group), the UVB irradiated group (B group), and the group treated with Lithospermum erythrorhizon extracts after UVB irradiation (C group). In the results of superoxidase dismutant(SOD) activities, the C group was meaningfully lower at the 48hrs, 120 hrs, and 168 hrs group than B group (p<0.05). In the result of catalase(CAT) activities, the C group was decreased at the 120 hrs and 168 hrs group, but meaningless (p>0.05). In the result of light micrograph observation, the B group observed sunburn cells in the epidermis and acute inflammation in the dermis at the 24 hrs and 48 hrs. And proliferation of the epidermis and the stratum granulosum, and found the hyperkertosis at the 72 hrs, 120 hrs, and 168 hrs group than C group. The C group alleviated inflammation in the dermis than B group at the 24 hrs and 48 hrs. And inhibited the proliferation of the epidermis at the 72 hrs, 120 hrs, and 168 hrs groups than B group. In conclusion, Lithospermum erythrorhizon water extracts may protect the UVB-damaged mouse skin.
Analysis of Chemical Composition, Microstructure and Hydroxyapatite Structure for Mouse Teeth
Kim, Eun-Kyung ; Jeon, Tae-Hoon ; Kim, Chang-Yeon ; Nam, Seung-Won ; Song, Kyung ; Lee, Sang-Gil ; Kim, Youn-Joong ;
Applied Microscopy, volume 40, issue 3, 2010, Pages 147~154
The aim of this study is to determine microstructure, chemical composition and crystal structure of hydroxyapatite for mouth teeth using optical microscopy and electron microscopy as well as electron probe micro-analysis (EPMA). Enamel, a protective cover to the teeth, consisted of rods oriented in regular and had relatively higher crystallinity and Ca component. In contrast, dentin showed a sponge-like microstructure with circular holes which were passages of dentinal tubules, and had higher Mg component than the enamel region due to its higher organic content. Hydroxyapatite crystals appeared as large rods in enamel, but as small needles in dentin. Their electron diffraction patterns were different by their crystallinity as well as by the organic content of the matrix.
Microstructure Analysis of Rabbit and Chicken Femurs by Light Microscopy and Transmission Electron Microscopy
Kim, Chang-Yeon ; Kim, Eun-Kyung ; Jeon, Tae-Hoon ; Nam, Seung-Won ; Kim, Youn-Joong ;
Applied Microscopy, volume 40, issue 3, 2010, Pages 155~162
Bone is a hierarchically structured composite material which has been well studied by the materials engineering community because of its unique structure and mechanical properties. Bone is a laminated organic-inorganic composite composed of primarily hydroxyapatite, collagen and water. The main mineral that gives bone's hardness is calcium phosphate, which is also known as hydroxyapatite. Light microscopy (LM) and transmission electron microscopy (TEM) were used to study the structure of femurs from chicken and rabbit. The elemental analysis was used to search variation in the distribution of calcium, potassium and oxygen in the femur. Current investigation focused on two structural scales: micro scale (arrangement of compact bone) and nano scale (collagen fibril and apatite crystals). At micro scale, distinct difference was found in microstructures of chicken femur and rabbit femur. At nano scale, we analyzed the shape and size of apatite crystals and the arrangement of collagen fibril. Consequently, femurs of chicken and rabbit had very similar chemical property and structures at nano scale despite of their different species.
Thermal Analysis of the Straight Permed Hair
Rho, Jung-Ae ; Lee, Gui-Young ; Chang, Byung-Soo ;
Applied Microscopy, volume 40, issue 3, 2010, Pages 163~168
We investigated the burning process of the virgin hair and straight permed hair using thermal analyser and scanning electron microscopy. After the thermal analysis, we found that weight started to change from
. Whereas straight permed hair has weight decrease rate of 2.6% higher than the virgin hair till
, those were 64.9% and 64.4% for the virgin and straight permed hairs, respectively at
, the weight decrease rate of the virgin hair became lower than the other; they were 32.3% and 33.4% for the virgin and straight permed hairs, respectively. Final values were 25.4% and 28.3% at
, which showed that final weight of burnt straight permed hair was higher than the virgin hair by about 2.9%. In terms of morphological changes, the hair started to melt together with adjacent ones by burning till
. The medulla and cortex were melt and the inside was empty like bamboo. The hair burnt till
was entirely carbonized and found as a clod. We found many blowholes at the surface of the hair clump.
Spermatogenesis of Black Molly and Sailfin Molly (Poeciliidae, Teleostei)
Ryu, Seung-Jun ; Kim, Dong-Heui ;
Applied Microscopy, volume 40, issue 3, 2010, Pages 169~176
Black molly (Poecilia sphenops) and sailfin molly (Poecilia latipinna) are a teleost belonging to Poeciliidae. The spermatogenesis between two species were investigated by light and electron microscope. The whitish testes of both black molly and sailfin molly were located between intestine and air bladder. The size of testis was major axis 7 mm, minor axis 2 mm. The testis contained numerous testicular cysts. In both black molly and sailfin, primary spermatocytes were comparatively large ellipsoidal, and mitochondria showed a marked development. The secondary spermatocyte was smaller than that of primary spermatocyte, highly condensed according to their development. The nucleus with electron-dense was round shape and flagella started to be formed. In spermiogenesis, chromatin was more condensed. The mitochondria were rearranged along the tail. The number of mitochondria was 2 to 4 in cross section and 8 to 10 in longitudinal section. The head of mature sperm was long cone shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm have lateral fins. In conclusion, spermatogenesis and sperm morphologies of these two species were same. These morphological similarity seems to be an indication of the Poeciliidae.