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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 41, Issue 4 - Dec 2011
Volume 41, Issue 3 - Sep 2011
Volume 41, Issue 2 - Jun 2011
Volume 41, Issue 1 - Mar 2011
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Nano Scale Compositional Analysis by Atom Probe Tomography: I. Fundamental Principles and Instruments
Jung, Woo-Young ; Bang, Chan-Woo ; Gu, Gil-Ho ; Park, Chan-Gyung ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 81~88
Even though importance of nano-scale structure and compositional analysis have been getting increased, existing analysis tools have been reached to their limitations. Recent development of Atom Probe Tomography (APT), providing 3-dimensional elemental distribution and compositional information with sub-nm scale special resolution and tens of ppm detection limit, is one of key technique which can overcome these limitations. However, due to the fact that APT is not well known yet in the domestic research area, it has been rarely utilized so far. Therefore, in this article, the theoretical background of APT was briefly introduced with sample preparation to help understanding APT analysis.
Nano Scale Compositional Analysis by Atom Probe Tomography: II. Applications on Electronic Devices and Nano Materials
Jung, Woo-Young ; Bang, Chan-Woo ; Jang, Dong-Hyun ; Gu, Gil-Ho ; Park, Chan-Gyung ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 89~98
Atom Probe Tomography (APT) can provide 3-dimensional information such as position and chemical composition with atomic resolution. Despite the ability of this technique, APT could not be applied for poor conductive materials such as semiconductor. Recently APT has dramatically developed by applying the laser pulsing and combining with Focused Ion Beam (FIB). The invention and combination of these techniques make possible site-specific sample preparation and permit the investigation of various materials including insulators. In this paper, we introduced the recently achieved state of the art applications of APT focusing on Si based FET devices, LED devices, low dimensional materials.
Fine Structure of Sperm in the Korea Squirrel, Tamias sibiricus
Lee, Jung-Hun ; Park, Ki-Ryong ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 99~107
Light microscope, and scanning and transmission electron microscopy were used to investigate the fine structure of sperm of the Korea squirrel, Tamias sibiricus. The sperm head of T. sibiricus was paddle in shape. The total length of T. sibiricus sperm was 67.8
. The length of sperm head was 7.8
, and the tail (60.0
) was consisted of four major segments: the neck (1.0
), middle (8.0
), principal (48.5
) and end piece (2.5
), respectively. Especially, the length of the middle piece is short, and end piece was very shorter than those of other rodents. The post-nuclear cap was occupied about a fifth of nucleus. The equatorial segment is located between the post-nuclear cap segment and acrosomal cap on the nuclear surface. Nine segmented columns were surrounded by the mitochondria, and numbers of gyres of mitochondria were 26. One segmented column was consisted ten to twelve knobs, and each of segmented column in the neck region connected with the nine outer dense fiber in the middle piece. Numerous satellite-like fibers were scattered around the segmented columns. Nos. 1, 5 and 6 of the outer dense fibers in the middle piece were larger than the others. A fibrous sheath and longitudinal column of the principal piece were in evidence, but the fibrous sheath and longitudinal column was not seen at the end piece. In conclusion, the structural features of sperm head and tail may be useful information to patterns of sperm evolution and classification of species.
Expression of NGF in Estradiol Valerate-Induced Polycystic Ovary and CHO Cells
Choi, Baik-Dong ; Jeong, Soon-Jeong ; Jeong, Moon-Jin ; Lim, Do-Seon ; Lee, Soo-Han ; Kim, Seung-Hyun ; Go, A-Ra ; Kim, Se-Eun ; Kang, Seong-Soo ; Bae, Chun-Sik ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 109~116
Polycystic ovary syndrome (PCOS) is hormonal imbalance condition as the endocrine and metabolic disorder that induces the infertility and various complications in reproductive age women. Estradiol valerate (EV) is used hormone replacement therapy in menopausal women and is reported that excessive administration of EV induces the PCOS. Nerve growth factor (NGF) is the factor to regulate the survival and maturation of developing neuronal cell and is also synthesized in ovary. And NGF is overexpressed in EV-induced polycystic ovary (PCO) as previously reported. Therefore, this study examined the possibility of NGF as can be used the biological marker in diagnosis of PCOS, the hormonal imbalance condition, using PCO and CHO (chinese hamster ovarian) cell lines. The concentration of EV treatment is optimized a 1 mg as not influence on the proliferation of CHO cell but 2 mg and 3 mg of EV treatment have the inhibition effect at initial stage. The morphological change was not observed in CHO cell after dose dependent manner treatment of EV. Expression of NGF mRNA and protein is significantly increased at 30 min after EV treatment in CHO cells compared to that of control. And NGF protein expression is strongly increased in PCO tissue, which observed many follicular cysts compared to normal ovary tissue. Taken together, overexpression of NGF may be act as a molecule to induce an abnormal development of follicle, suggesting that NGF can be used as a biological marker in diagnosis of PCOS.
Sperm Ultrastructure of Pipistrellus savii velox
Lee, Yu-Ri ; Lee, Jung-Hun ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 117~122
Sperm morphology of Pipistrellus savii velox was examined by transmission electron microscope. The sperm head of P. savii velox was bullet in shape. The sperm head was 3.1
in length, whose posterior 3.0
was occupied by a nucleus with 1.8
in width. The segmented columns were about 14~15 in number. The total number of mitochondrial gyres was 57. Number of 1, 5, and 6 of the outer dense fibers were larger than the others. A fibrous sheath and longitudinal column of the principal piece were evidence, but the fibrous sheath was not seen at the end piece. In the present study, the length of the sperm head of P. savii velox were very shorter than those of other bats.
Ultrastructural Variations on the Micropyle of Blacktip Grouper, Epinephelus Fasciatus before and after Artificial Fertilization
Kim, Seong-Hoon ; Lee, Chi-Hoon ; Ju, Hea-Sung ; Kim, Hyung-Bae ; Lee, Young-Don ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 123~128
The micropyles on the prefertilized and artificial fertilized eggs of Epinephelus fasciatus were investigated using scanning electron microscope (SEM). The micropyles (
) of E. fasciatus eggs were found in the animal pole and theirs shape were observed as a flat crateriform of cylindrical shape. The micropylar vestibule arranged by 6~7 thickened spiral annuli on the ridge and contributed to differentiate and form fertilization cone for blocking to polyspermy by presenting swollen vestibule structure. As E. fasciatus eggs was pelagic, so chorionic surface was an uneven structures such as circular and fillar form nodules. Especially, various pores (0.15~0.55
, 230~270 pores) distributed at the only around micropyles, those pores radiately exhibited regular projection structures showing gill filament-shape. These ultrastructural characters of E. fasciatus eggs can be utilized in a taxonomical cue of grouper species.
Inhibitory Effects of Nude Pack Containing Black Tea Water Extract on Skin Wrinkle Formation in Hairless Mice
Kim, Young-Chul ; Park, Eun-Ye ; Kim, Sang-Nam ; Yoo, Yong-Gi ; Park, Mi-Soon ; Lee, Gui-Yeong ; Lee, Suk-Jun ; Chang, Byung-Soo ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 129~137
The aim of this study was to evaluate the inhibitory effect of nude pack containing black tea water extract (NPBT) on skin wrinkle formation in hairless mice. Skin wrinkles were induced by UVB irradiation to the backs of hairless mice for 5 weeks. And at the same time, NPBT was applied topically. Wrinkle formation, histological changes, expression of matrix metalloproteinase-3 (MMP-3) and protein activities of MMP-2 and MMP-9 were observed or analyzed. Wrinkles for the control group were formed as a pattern of deep furrows and thick crests. Whereas wrinkles for the NPBT treated group were formed as a pattern of shallow furrows and thin crests, and their wrinkle areas were significantly (p<0.001) lower than the control group. Collagen fibers were arranged irregularly and sparse in density and some elastic fibers were degenerated in the control group, while they were almost intact in the NPBT treated group. MMP-3 mRNA expression in the control group was significantly (p<0.001) higher than the normal group, and that of NPBT treated group was significantly (p<0.001) lower than the control group. The NPBT treated group showed remarkably lower protein activities of MMP-2 and MMP-9 than the control group. NPBT could have a considerable inhibitory effect on skin wrinkle formation in hairless mice.
Characteristics of Adhesive Disks in Parthenocissus tricuspidata during Attachment
Lee, Myung-Hui ; Kim, In-Sun ;
Applied Microscopy, volume 41, issue 2, 2011, Pages 139~145
Parthenocissus tricuspidata is an epiphyte that lacks a main axial stem, but develops adhesive disks along the stem for climbing support. In this study, scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were utilized to examine the brick wall surface and the adhesive disks of P. tricuspidata that attached to the surface successfully. The study was mainly focused the outermost layers of both structures before and after adhesion to find out whether there has been some structural and/or physical interactions between the two. The adhesive disks adhered firmly to the brick wall by secreting adhesive materials that help them for a tight attachment to the surface. The rough wall surface appeared facilitating better attachment of the adhesive disks by infiltrating the materials into those spaces leading to some degree of interactions at the interface. EDS analysis on the outermost layers of the adhesive disks that were separated from the substrates was also consistent with the SEM data on the interaction between the adhesive disks and the substrate surface. EDS analysis of the brick wall surface and the adhesive disks demonstrated similar elements of O, Si, Fe, Al, K, Mg, and Na in their components.