Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society of Electron Microscopy
Editor in Chief :
Volume & Issues
Volume 42, Issue 4 - Dec 2012
Volume 42, Issue 3 - Sep 2012
Volume 42, Issue 2 - Jun 2012
Volume 42, Issue 1 - Mar 2012
Selecting the target year
Ultrastructural Abnormalities in APP/PSEN1 Transgenic Mouse Brain as the Alzheimer's Disease Model
Kim, Mi Jeong ; Huh, Yang Hoon ; Choi, Ki Ju ; Jun, Sangmi ; Je, A Reum ; Chae, Heesu ; Lee, Chulhyun ; Kweon, Hee-Seok ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 179~185
DOI : 10.9729/AM.2012.42.4.179
Alzheimer's disease (AD) is a progressive neurodegenerative disorder. Neuropathological hallmarks of AD are amyloid plaques, dystrophic neurite, and alteration of subcellular organelles. However, the morpho-functional study of this degenerative process and ultimate neuronal death remains poorly elucidated. In this study, immunohistochemical and ultrastructural analyses were performed to clarify the abnormal morphological alterations caused by the progression of AD in APP/PSEN1 transgenic mice, express human amyloid precursor protein, as a model for AD. In transgenic AD mice brain, the accumulation of Amyloid
plaques and well-developed dystrophic neurites containing anti-LC3 antibody-positive autophagosomes were detected in the hippocampus and cortex regions. We also found severe disruption of mitochondrial cristae using high-voltage electron microscopy and three-dimensional electron tomography (3D tomography). These results provide morpho-functional evidence on the alteration of subcellular organelles in AD and may help in the investigation of the pathogenesis of AD.
Comparative Ultrastructure of Secretory Granules of the Submandibular Gland in the Korean Spider Shrew, Sorex caecutiens, the Lesser White-toothed Shrew, Crocidura suaveolens and the Big White-toothed Shrew, Crocidura lasiura
Jeong, Soon-Jeong ; Bae, Chun Sik ; Lee, Hye-Yon ; Choi, Baik-Dong ; Yoon, Myung-Hee ; Jeong, Moon-Jin ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 186~193
DOI : 10.9729/AM.2012.42.4.186
The ultrastructure of the secretory granules of the submandibular gland was examined in the Korean spider shrew, Sorex caecutiens, lesser white-toothed shrew, Crocidura suaveolens and big white-toothed shrew, C. lasiura. The mucous and serous acinar granules of S. caecutiens with a border of the lucid corona differed from those of C. suaveolens and C. lasiurar with a dense lateral border. The mucous acinar granules of C. lasiura with several bands producing a variety of patterns in the matrix were similar to those of C. suaveolens. The serous acinar granules of C. lasiura had a homogenous pale center surrounded by minute dense specks or had an unusual substructure showing a geometric pattern according to the inclusions in the pale matrix of the granules. This is the first report of an unusual substructure showing a geometric pattern of the serous acinar granules of C. lasiura. The myelin-like body was observed in the granular duct cell of the three species of shrew. The myelin-like body of S. caecutiens with layers of unit membranes was different from that of C. suaveolens and C. lasiura with paired membranes. Therefore, the layers composing of the paired membranes of the myelin-like body might be one of the characteristics of Crocidura.
Anatomical and Morphological Features of Seeds in Portulaca
Kim, InSun ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 194~199
DOI : 10.9729/AM.2012.42.4.194
The anatomy and morphology of seeds from 10 Hawaiian Portulaca taxa were examined to explore patterns of variation among the taxa, and to evaluate their phylogenetic relationships. Features of seeds were assessed employing microtechnique procedures, statistical analysis, and scanning electron microscopy (SEM). Anatomically, the orientation of embryo was consistent across taxa, and all seeds examined had central nutritive tissue and integuments comprising the peripheral embryo. Seeds were generally small, circular to ovoid in shape, and either smooth or rough-surfaced, with tubercules. Variation in seed size was noted, although minimal within small seeded group. The size of seeds ranged from 0.50~1.26 mm in length, and 0.55~1.34 mm in width, with similar weights. Seeds were distinguished by the sculpture and arrangement of the testa epidermis and the way in which their stellulate-tessellate cells uniquely fit together. Using a multiple range test (ANOVA), two groups were established by seed characteristics. The SEM also demonstrated morphological differences in testa sculpturing. The results obtained confirm the usefulness of seed characteristics in the identification of the species examined, and furthermore, allow for the separation of the Hawaiian Portulaca into two groups.
Changes in the Neurogenesis and Axonal Sprouting in the Organotypic Hippocampal Slice Culture by Aβ
Jung, Yeon Joo ; Jiang, Hui Ling ; Lee, Kyung Eun ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 200~206
DOI : 10.9729/AM.2012.42.4.200
Induction of neurogenesis can occur in the hippocampus in response to various pathological conditions, such as Alzheimer's disease. The aim of this study was to investigate the changes that occur in endogenous neural stem cells in response to amyloid beta
-induced neuronal cell damage in organotypic hippocampal slice cultures. Cresyl violet staining and Fluoro-Jade B staining were used to detect neuronal cell damage and changes of mossy fiber terminals were observed by Timm's staining. The immunofl uorescence staining was used to detect the newly generated cells in the subgranular zone (SGZ) of the dentate gyrus with specific marker, 5-bromo-2'-deoxyuridine (BrdU), Ki-67, Nestin, and doublecortin (DCX). In compared to control slices, neuronal cell damage was observed and the mossy fibers were expanded to CA3 area by treatment with
. Ki-67/Nestin- and BrdU/DCX-positive cells were detected in the SGZ. In conclusion, these results demonstrate that
-induced neuronal damage results in an increase in endogenous neural stem cells in rat hippocampal slice cultures not only for gliosis but also for neurogenesis.
Synthesis and Characterization of a Pt/NiO/Pt Heterostructure for Resistance Random Access Memory
Kim, Hyung-Kyu ; Bae, Jee-Hwan ; Kim, Tae-Hoon ; Song, Kwan-Woo ; Yang, Cheol-Woong ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 207~211
DOI : 10.9729/AM.2012.42.4.207
We examined the electrical properties and microstructure of NiO produced using a sol-gel method and Ni nitrate hexahydrate (
) to investigate if this NiO thin film can be used as an insulator layer for resistance random access memory (ReRAM) devices. It was found that as-prepared NiO film was polycrystalline and presented as the nonstoichiometric compound
with Ni interstitials (oxygen vacancies). Resistances-witching behavior was observed in the range of 0~2 V, and the low-resistance state and high-resistance state were clearly distinguishable (
orders). It was also demonstrated that NiO could be patterned directly by KrF eximer laser irradiation using a shadow mask. NiO thin film fabricated by the sol-gel method does not require any photoresist or vacuum processes, and therefore has potential for application as an insulating layer in low-cost ReRAM devices.
Effects of Post Annealing on the Electrical Properties of ZnO Thin Films Transistors
Moon, Mi Ran ; An, Chee-Hong ; Na, Sekwon ; Jeon, Haseok ; Jung, Donggeun ; Kim, Hyoungsub ; Lee, Hoo-Jeong ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 212~217
DOI : 10.9729/AM.2012.42.4.212
This paper reports the effects of post-annealing of ZnO thin films on their microstructure and the device performance of the transistors fabricated from the films. From X-ray diffraction and transmission electron microscopy characterization, we uncovered that the grain size increased with the annealing temperature escalating and that the film stress shifted from compressive to tensile due to the grain size increment. Electrical characterization revealed that the grain size increase damaged the device performance by drastically lifting the off-current level. By annealing the devices in an
ambient (instead of air), we were able to suppress the off-current while improving the electron mobility.
Atomic Resolution Imaging of Rotated Bilayer Graphene Sheets Using a Low kV Aberration-corrected Transmission Electron Microscope
Ryu, Gyeong Hee ; Park, Hyo Ju ; Kim, Na Yeon ; Lee, Zonghoon ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 218~222
DOI : 10.9729/AM.2012.42.4.218
Modern aberration-corrected transmission electron microscope (TEM) with appropriate electron beam energy is able to achieve atomic resolution imaging of single and bilayer graphene sheets. Especially, atomic configuration of bilayer graphene with a rotation angle can be identified from the direct imaging and phase reconstructed imaging since atomic resolution Moir
pattern can be obtained successfully at atomic scale using an aberration-corrected TEM. This study boosts a reliable stacking order analysis, which is required for synthesized or artificially prepared multilayer graphene, and lets graphene researchers utilize the information of atomic configuration of stacked graphene layers readily.
Electrical Characterization of Electronic Materials Using FIB-assisted Nanomanipulators
Roh, Jae-Hong ; You, Yil-Hwan ; Ahn, Jae-Pyeong ; Hwang, Jinha ;
Applied Microscopy, volume 42, issue 4, 2012, Pages 223~227
DOI : 10.9729/AM.2012.42.4.223
Focused Ion Beam (FIB) systems have incorporated versatile nanomanipulators with inherent sophisticated machining capability to characterize the electrical properties of highly miniature components of electronic devices. Carbon fibers were chosen as a model system to test the applicability of nanomanipulators to microscale electronic materials, with special emphasis on the direct current current-voltage characterizations in terms of electrode configuration. The presence of contact resistance affects the electrical characterization. This resistance originates from either i) the so-called "spreading resistance" due to the geometrical constriction near the electrode - material interface or ii) resistive surface layers. An appropriate electrode strategy is proposed herein for the use of FIB-based manipulators.