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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume 6, Issue 1 - Oct 1976
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Ultrastructural Studies of Typhoid Cells
Kim Chung-Sook ; Lee Yoo-Bock ; Kim Dong-Sik ;
Applied Microscopy, volume 6, issue 1, 1976, Pages 1~7
To investigate the nature of typhoid cells, three cases of clinically, serologically and histopathologically proven typhoid lesions of the small intestine and regional lymph nodes were studied light and electron microscopically, Light microscopically, typhoid cells were swollen mononuclear cells characterized by abundant amount of eosinophilic cytoplasm and frequent phagocytoses of red blood cells, bacterial clumps and other tissue debris. These cells were pyronin negative, Electron microscopically, these cells showed marked and diffuse dilatation of RER cisternae and disappearance of ordinary cytoplasmic organelles, but frequent phagocytosed materials, The meaning and reason of RER cisternal dilatation and reduction of cytoplasmic organelles were discussed, and are regarded as degenerative process due to bacterial endotoxin. Although there was hot enough cytoplasmic organelles to pinpoint the origin of typhoid cells, active phagocytosis and evidences against being either plasmacytic or lymphocytic nature favored retuculoendothelial nature of the typhoid cells.
Histochemical and Ultrastructural Investigation of the Mucus Containing Cells of the Gastrointestinal Tract in the Mice
Kim, Doo-Kyu ; Kim, Woo-Kap ;
Applied Microscopy, volume 6, issue 1, 1976, Pages 9~20
The present study was performed to clarify the histochemical compositions and fine structure of the mucus secreting cells in the gastrointestinal mucosa of normal mice. The mucus cells in the surface epithelium of stomach body had neutral mucin and some quantity of weak acid mucin. And the mucus cells in gastric pits and mucus neck cells had neutral mucin. The goblet cells in villial epithelium of small intestine contain strong acid sulfated mucin as their main content and a little of neutral mucopolysaccharide. However, the goblet cells in intestinal glands-Liberkuhn crypt were confirmed to contain non-sulfated weak acid mucin. The goblet cells in the surface epithelium of colon had the same component as the small intestine did. But the cells in the crypts of colon contained neutral and weak acid mucin as their main contents. The majority of secretory granules of the surface epithelial cells of the stomach body had high electron density, and some granules with low electron density appeared too. While the mucin granules in the mucus neck cells were low in its electron density, and some of those granules were frequently found to have dense core in them. Secretory granules in goblet cells of small and large intestines had low electron density. The mode of secretion in mucin-containing cells in gastro-intestinal tract was found to be merocrine.
Multivesicular bodies 및 Cytolysomes에 관(關)한 전자현미경적(電子顯微鏡的) 연구(硏究)
Kim, Woo-Kap ; Kim, Chang-Whan ; Park, Hong-Duok ; Yang, He-Young ;
Applied Microscopy, volume 6, issue 1, 1976, Pages 21~32
The origins and the functions of the multi vesicular bodies and the various structures of the membranes related to the cytolysomes were studied in the mycelium cells of Rhizopus nigricans, Aspergillus niger and A. ochraceus, in the hymenium and basidium cells of Agricus bisporus sand Rhizopogon rubesecens, in the cells of assimilation tissue of Marchtantia polymorpha and Pogonalum inflexum and in the mesophyll cells of Pteridium aqiulinum, Pinus densiflora, Ginkgo biloba and Panax ginseng fixed with glutaraldehyde-paraformaldehyde-
. In Rhizopus nigricans, Aspergillus niger, A. ochraceus, Agricus bisporus sand Rhizopogon rubescens, the concentric multilamellar, multivesicular, myelin-vesicle-tubular and concentric parallel-lamellar complexes were originated from the plasmalemma, while in Marehantia polymorpha, Pogonatum inflexum, Pteridium aquilinum, Pinus densiflora, Ginkgo biloba and Panax ginseng, they were originated from plasmalemma and the cytoplasm. The structures originated from the plasmalemma may be grouped into multi vesicular body and myelin-like structure, both forming the secondary vacuoles or protruding into the central vacuoles and finally degrading, In some cases, endoplasmic reticulum within the cytoplasm encloses some part of the cytoplasm to form a circle where the membranous lamellae increase in number, while the enclosed cytoplasm decrease to be eventually replaced by the multilamellar structure which is released into the vacuoles and subsquently degraded. The structures originated from the cytoplasm are believed to be the cytosegresomes or cytolysomes closely related to the differentiation of the vacuoles. The possible fate of these structures are also discussed.
Defect Formation in Epitaxially Grown Silver Films
Lee Ki-Seon ; Kim Key-Soo ;
Applied Microscopy, volume 6, issue 1, 1976, Pages 33~38
Formation of crystal defects which may occur during and after the vacuum deposition of silver films on rocksalt substrates were studied by electron microscopy. To obtain defect free films, various evaporation conditions, such as evaporation rate, substrate temperature, substrate treatments and annealing of films were examined. Stacking faults and micro-twins are dominant defects in silver films.