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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume 8, Issue 1 - Apr 1978
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Mode of Action of Antituberculosis Drugs and Ultrastructure of Mycobacterium tuberculosis
Deung, Young-Kun ; Cheung, Sung-Douk ; Kim, Sung-Kwang ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 1~8
The aim of this research was to discover the action mechanism of various antituberculosis agents (isoniazid, paraaminosalicylic acid and streptomycin) which act on Mycobacteria tuberculosis hominis
and also to study the relationship of ultrastructural changes and the growth pattern in Mycobacterium tuberculosis hominis. The ultrastructural change was observed with an electron microscope while the growth pattern was studied through in vitro culture. The results are summarized as follows: 1. The ultrastructural changes found in the group treated only with isoniazid were the loss of nuclear materials and the appearence of electron dense granules. 2. In the group treated with paraaminosalicylic acid, thickening of nuclear filaments and meso some arrangement disorders were observed. 3. In the group treated with streptomycin, the ribosome particles appeared indistinct and the cytoplasm was denaturalized. 4. In the group cross treated with all three agents, all the ultrastructural changes mentioned above could be observed in the cell just as they appeared in the single treated groups. 5. In all of the single and in the crossly treated group, there were no significant changes note in the cell wall or cytoplasmic membranes of any of the cells observed. 6. In the cultural data in vitro, through the crossly treated group and single treated group. growth was observed in 3-5 weeks of culture.
Electron Microscopical Observation on the Flagella of Several Species of the Genus Rhizobium
Ahn, Kyung-Joon ; Lee, Woong-Jik ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 9~15
Five strains of the Genus Rhizobium were isolated from the nodules of five leguminous plants respectively. They were identified according to Bergey's Manual together with the results of Vincent. The flagella of each strains were observed by electron microscope using negative staining with PTA and metal shadowing with chromium. Five host plants and identified Rhizobium strains were as. follows. Pisum sativum.....R. leguminosarum Phaseolus vulgaris.....R. phaseoli Trifolium repens.....R. trifolii Glycine max.....R. japonicum Lupinus grandiflorus.....R. lupini Electron micrographs showed that R. leguminosarum and R. phaseoli had 4 peritrichous flagella, where as R. trifolii had 5 peritrichous flagella. On the other hand, R. japonicum and R. lupini had 1 subpolar flagellum.
Ultrastructural Study of the Histiocytosis X. -Report of two cases-
Kim Chung-Sook ; Lee Yoo-Bock ; Kim Kir-Young ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 17~22
Two cases of Histiocytosis X, a Hand-Schuller-Christian disease and a Letterer-Siwe disease, are,examined light and electron microscopically. Many of the histiocytes without appreciable lipid contained numerous rod-shaped structure identical to Langerhan's granules in the cytoplasm. The significance of this structure was discussed together with review of literature.
An Ultrastructural Study on Larval Hemocytes of Orthoptera
Nam, Sang-Ho ; Yang, He-Young ; Kim, Woo-Kap ; Kim, Chang-Whan ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 23~31
An electron microscopical study on hemocytes of last instar larvae of Orthoptera (Acrida cinerea Thunberg, Paratenodera sinensis De Saussure, Atractomorpha lata Hotschulsk was performed to observe cell types, structures and their origins. Prohemocytes are the smallest round cells with large, nucleus, poorly developed cytoplasmic organelles and lower composition ratio, accouting for less than 1% of all cell types. Little remarked differences were revealed among different species. Plasmatocytes are relatively large cells with higher composition ratio, accounting for 5 to 20%. The majority of the plasmatocytes are oval forms with numerous external cytophismic projections., relatively well developed cytoplasmic organelles. Granular cells are characterized by various granules in cytoplasm. Some considerable remarked differences were revealed among different species. Furthermore the granules themselves differ in fine structures. Adipohemocytes were found only in Acrida cinerea Thunberg.
Cadmium 중독에 의한 붕어 (Carassius carassius L.) 장기의 미세구조 변화
Yu Gwan-Hui ; Choe Chun-Geun ; Choe Im-Sun ; Deung Yeon-Geon ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 33~48
Electron Microscopic Study of the Mast Cells of the Bat Stomach
Kang Ho-Suck ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 49~52
Mask cells are distributed in the animal tissue, The bat subject has not been studied with the electron microscope, The material was fixed in 2.5% phosphate buffered glutaraldehyde for 24 hours at
and then post-fixed in phosphate buffered 1% osmium tetroxide for 2 hours at
and then the cleared tissues were embedded in Epon. The mast cell has numerous cytoplasmic processes projecting into the surrounding connective tissue. In general, the cytoplasmic granules showed ovoid, round, lunal or irregular shape, most of them were fine particulate substructure in texture. Especially, the granules was enclosed by the lamella structure.
The Synaptic Organization of the Cat Striatum
Chung Jin-Woong ; Choi Wol-Bong ; Kwun Hung-Sik ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 53~66
An attempt has been made to discriminate the synapses in the striatum consisting caudate nucleus, putamen and fundus striati of the cat with emphasis on the characteristic structures of axon terminals and postsynaptic profiles. The differentiation is based on the size and shape of vesicle in the bouton terminal, and the symmetrical or asymmetrical thickening the pre- and postsynaptic membrane. Four types of synapses could be differentiated: Type I: the bontons with asymmetrical,synaptic thickenings contain round 45 nm diameter vesicles and contact cell soma, dendritic shafts and dendritic spines (74%). Type II : the boutons contain round 45nm diameter vesicles and are associated with symmetrical membrane thickenings. These synapses are formed on the soma and dendritic shafts (6%). Type III: the boutons with symmetrical membrane thickenings contain 50-60 nm diameter pleomorphic vesicles, and contact soma and dendritic shafts (18%). Type IV: the terminals contain flattened vesicles (
) and are associated with symmetrical membrane thickenings. These synapses are found in contact with soma and dendritic shafts. Additionally, the bouton en passant, which is expanded from myelinated or unmyelinated axons containing round vesicles (45nm diameter) contacts the dendritic shaft or dendritic spine with asymmetrical membrane thickenings. Two unusual types of synapses, axo-axonic and dendro-dendritic, are found occasionally.
A Comparative Study of Fine Structure of Callus Cells in Panax ginseng
Lee, Chai-Doo ; Lee, Kyu-Bae ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 67~76
A comparative investigation of fine structure of callus cells derived from tissue culture of Panax ginseng was made by electron microscope. Callus was consisted of large superficial cells and small inner zone cells derived from shoot apex tissue cultured for 16 weeks. Large superficial cells were contained the clusters of starch grains surrounded by a double plastid membrane. Especially, electron dense particles were deposited just inside and outside of plastid membrane and also deposited on mitochondria-like and endoplasmic reticulum-like structures. Crystalline body was also found in superficial cells. Small inner zone cells were characterized by presence of proplastids sheathed by short endoplasmic reticulum profiles. presence of spiral configuration of ribosomes and absence of crystalline body. Organ primordia was consisted of a dense cytoplasm and notable nucleate cells derived from nodal tissue cultured for 67 weeks. Proplastids containing starch grains and crystalline bodies were frequently observed; starch grains are of small quantity and does not form the clusters as in inner zone cells; hexagonal crystalline body itself does not have always limiting membrane. Remarkably. in a few cells of primordia, particles resembling the presumptive virus were observed mainly in condensed nuclear chromatin and also in cytoplasm, in mitochondrion-like organelle.
Direct Observations of Al-Si Junction Interface
Lee Ki-Seon ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 77~79
Al-Si junctions were made by vacuum deposition of aluminium on to silicon wafers and examined by TEM. The uneven interfaces of the junctions are formed due to the surface tension of the molten solution resulting in preferential dissolution of silicon in aluminium at some areas. These undesirable uneven interfaces affect the junction shape and so the over-all characteristics of the devices.
Practical Application of Electron Microscopy in Clinical Medicine
Kim Chung-Sook ;
Applied Microscopy, volume 8, issue 1, 1978, Pages 81~90
Introduction of electron microscope in biological and medical sciences change the concept of functional and morphologic unit of biological phenomena from the cell to subcellular unit, and it formulated the basis for molecular biology and pathology. Until recently, electron microscopy has mainly been applied to basic research works. However, practical clinical application of electron microscopy is being actively tried. The major clinical fields in which electron microscopy is helpful or even essential include viral diseases, metabolic diseases, glomerular diseases and in the identification of certain types of neoplasms. A brief introduction of characteristics of each conditions are made to encourage more active application of electron microscopy in clinical medicine.