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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 9, Issue 1 - Apr 1979
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Electron Microscopical Observation on the Compound Eye in Meimuna mongolica D.
Choi, Choon-Keun ; You, Kwan-Hee ; Shin, Kil-Sang ; Choe, Rim-Soon ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 1~11
Electron microscopic studies were carried out to investigate the fine structure of ommatidia of the compound eyes in Meimuna mongolica D. The crystalline cone appears round, and is composed of four double-membraned cone cells and surrounded by pigment cells having many pigment granules. The rhabdom is a closed type, and is composed of four rhabdomeres Its cross section reveals lamellated microvilli which are oriented in four different directions suggesting that these represent four photoreceptive sites. The microvilli, in a cross sectional view, are hexagonal in shape with a central axis inside. There are usually eight retinular cells arranged radially from the rhabdom, but in some retinular layers seven or nine retinular cells could be observed. The cytoplasm of each retinular cell is interconnected with that of microvilli of the rhabdomere, but the appearance of this interconnection varies depending on the number of retinular cells.'The retinlilar cells neighboring the microvilli seem to have well-developed perirhabdomal vacuoles and mitochondria as well as pigment granules surrounding these vacuoles.
The Effect of Lead on the Growth of Carrot Callus: An Ultrastructural Study
Hwang, Baek ; Chung, Sang-Jin ; Kang, Young-Hi ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 13~19
The present work has been carried out mainly to determine the effect of lead on the growth and cell organelles of Carrot (Daucus carota L.) callus. 1 mM of lead nitrate is added to the culture media R-2 and callus cells are cultured for 16 days. The growth rate is measured by fresh weight and structural changes of cell organelles is observed by electron microscope at every 4 days. The results show that lead inhibits the growth of Carrot callus and disturbs the structure of mitochondria remarkably.
Cyclic Changes of the Epithelium of Seminiferous Tubule in the H rseshoe Bat (Rhinolophus ferrumequinum korai)
Oh, Yung-Keun ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 21~34
The cyclic changes of the epithelium of seminiferous tubule in the Korean greater horseshoe bat (Rhinolophus ferrumequinum korai) were investigated by means of the light and electron microscopic observations and the following results were obtained. 1) The spermatogenic function of the horseshoe bats captured in September was moderate but considerable amount of the ultrastructural findings were obtained in both the spermatocytogenic and the spermiogenic phases. 2) The spermatogenic function of the horseshoe bats captured in December was depressed representing a resting status. 3) The light and electron microscopic findings suggest that the epithelium of seminiferous tubule varies cyclically, which is closely related to their peculiar way of reproductive habit during hibernation.
Ultrastructural Studies of the Brain Tumors
Deung, Young-Kun ; Kim, Chung-Sook ; Lee, Kyu-Chang ; Lee, Hun-Jae ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 35~56
To investigate ultrastructural characteristics of cancer cells of the nervous system, 25 cases; i.e. astrocytoma(9), oligodendroglioma(1), medulloblastoma(1), meningioma(5), pinealoma(2) and pituitary adenomas(7). The common findings were marked irregularity of nuclear membrane with pronounced infoldings, clumping of heterochromatin along inner nuclear membrane, enlargement of nucleolus, and frequent observations of nuclear bodies and nuclear inclusions. But these findings are also the signs that can be observed in hyperactive cells. Thus, ultrastructural characteristics of cancerous nucleus are the great variability of nuclear size, shape and composition. but none of them appear to be specific. Among cytoplasmic organelles, massive fibrils are characteristic of astrocytoma and meningiomas, cytoplasmic protofibrils such as glial process and microvesicles in oligodendroglioma, secretory granules are characteristic in pituitary adenomas, and fine filamentous fibrils and desmosomes are characteristic of fibroblastic type of meningioma. Intercellular relationships and cell membrane specialization are important features in the differential diagnosis of various undifferentiated tumors. The frequent resolution of difficult diagnosis problems by electron microscopy outweighs the disadvantages of this technique, such as the expense and time required.
Ultrastructural Changes during Germination of Ginseng Seeds (Panax ginseng)
Kim, Woo-Kap ; Park, Hong-Duok ; Kim, Eun-Soo ; Han, Sung-Sik ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 57~69
The ultrastructural changes of embryo and endosperm cells were observed during the green fruit with embryo about
long to germination. 1. In the embryo cells of green fruit with embryo about
long, mitochondrial cristae and plastid are undifferentiated and dictyosome are occasionally observed. There are electron-opaque globoids in the vacuole and a lot of spherosomes in the outer layer of smooth endoplasmic reticulum. Endosperm is filled with spherosomes and electron-opaque protein bodies surrounded by spherosomes, and due to these, other organelle are not observed. 2. In the embryo cells of seeds with red seed coat, mitochondrial cristae are well developed, electron-opaque globoids increased, and vacuoles are enlarged. In the endosperm, however, spherosomes increased, protein bodies are enlarged, and electron-opaque globoidal crystals are dispersed within them. 3. In the procambium and epicotyl cells of dehiscent seed, Golgi vacuoles and vesicles are well developed, and mitochondrial cristae are also well differentiated. Spherosomes are numerously present and radicle cells, peripheral cells of hypocotyl, and vacuoles of cotyledon are well differentiated. Endosperm is filled with spherosomes containing electron-opaque granules and protein bodies are surrounded by a single membrane. There are acid phosphatase around globoids and spherosomes. 4. At the time of seeding, spherosomes markedly increased in the outer layer of cotyledon and protein bodies are also observed. Cell organelles are differentiated and plastids containing starch are also present. 5. In the outer
layers of cotyledons, radicle cells, and peripheral cells of hypocotyl during post-seeding to germination, spherosomes and plastids with starch increased, and mitochondria and microbodies are also found around the nucleus of embryo cells. With approaching, the germination stage, in the endosperm contacting with embryo, vacuoles are well differentiated but spherosomes decreased. There increased electron-opaque materials within vacuoles. In other endosperm, with the decrease of spherosome, mitochondria increased and electro n-opaque globular bodies are formed and gradually increased. The outer layer of protein bodies are reduced while electron-transparent portions are enlarged and fused together to occupy the outer layer where small particles are formed. 6. In the endosperm of germination stage, spherosomes decreased while protein bodies, are fused together to form 2 or 3 within a cell.
Immune Electron Microscopy
Choe, Jin ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 71~77
Cytochemistry for Electron Microscopy
Kim, Chung-Sook ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 79~86
Electron Microscope Radioautography
Choi, Wol-Bong ;
Applied Microscopy, volume 9, issue 1, 1979, Pages 87~94