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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Crop Science
Editor in Chief :
Volume & Issues
Volume 34, Issue s02 - Dec 1989
Volume 34, Issue 4 - Dec 1989
Volume 34, Issue 3 - Sep 1989
Volume 34, Issue s01 - Jun 1989
Volume 34, Issue 2 - Jun 1989
Volume 34, Issue 1 - Mar 1989
Selecting the target year
Need for Active Researches on the Crop Growth Regulation
Yong-Woong Kwon ;
Korean Journal of Crop Science, volume 34, issue s01, 1989, Pages 1~3
Bioassays of Plant Hormones and Plant Growth Regulating Substances I . Auxins, Gibberellins, and Cytokinins
Korean Journal of Crop Science, volume 34, issue s01, 1989, Pages 4~15
The objective of this paper is to compare and summarize the procedure and effectiveness of some bioassay systems and to point out ways to obtain reliable results from each bioassay. Detailed C:escriptions were given for those widely-adapted bioassay methods, such as mungbean rooting (auxin), Avena first internode straight growth (auxin), dwarf rice growth (gibberellin), dwarf pea epicotyl elongation (gibberellin), radish cotyledon expansion test (cytokinin), and tobacco stem pith callus growth (cytokinin), and the effects of various plant growth regulators including some recently introduced growth retardants (Paclobutrazol, Uniconazol, etc.) were also summarized.
Bioassays of Plant Hormones and Plant Growth Regulating Substances II. Abscisic Acid and Brassinolide
Korean Journal of Crop Science, volume 34, issue s01, 1989, Pages 16~25
A bioassay is a test system using a living organism (in whole or in part) to determine the presence or relative potency of chemical substances. The development and uses of bioassay are intimately linked to the discovery and characterization of the major classes of plant hormones. An application of this relationship is helpful for understanding the concept of plant hormones as well as the use of bioassay. And plant bioassay have been development and employed not only for the discovery and characterization of the biological activity of plant growth regulators but also have served several important secondary roles. The ideal bioassay should possess the characteristic of high specificity. great sensitivity. short response time, low cost and ease of obtaining plant material. acceptable ease of manipulation, and minimal space and equipment requirements.
Analysis of Plant Hormones using GC-MS
Korean Journal of Crop Science, volume 34, issue s01, 1989, Pages 26~32
The analytic principles of GC and MS were explained in relation to plant hormone analyses and the characteristics of two instruments were compared. The selection of column, condition of measurement and the method of ionization to get a good spectrum were also briefly described. Finally, the pre-treatment of sample by solvent extraction method to remove the unnecessary part of sample and the synthetic method, especially reagents and reaction condition, for the preparation of ether or ester derivative which can be easily vaporized in GC were explained.
Procedures for Analyzing Ethylene by Gas Chromatograph
Korean Journal of Crop Science, volume 34, issue s01, 1989, Pages 33~39
Ethylene gas classified as one of five major plant hormones plays an important role in various plant metabolism. The precise analysis of ethylene production of plants or plant parts is a valuable research procedure because knowledge of ethylene production facilitates measures of the physiological activity within the tissue. This paper describes procedures for analyzing ethylene from plant tissues by gas chromatography and discusses problems associated with extracting gas samples either by introducing a vacuum to plant samples or by using a hypodermic syringe. Introduced are a continuous flow system for efficient analysis and an automated system for sampling, analyzing, calculating and recording ethylene production data.
Enzyme-linked Immunosorbent Assay of Plant Hormones
Korean Journal of Crop Science, volume 34, issue s01, 1989, Pages 40~47
In spite of the development of highly sophisticated instrument, the precise quantitation of plant hormones still has many difficulties. Due to their high specificity, sensitivity and minimal sample purification steps, immunological assays have been widely applied for plant hormone assay. Enzme-linked immunosorbent assay technique for the determination of plant hormones was developed by Voller in 1978. Immunological assays are accomplished by competition of labeled tracer antigen and unlabeled antigen for a limited number of specific antibodies. The use of enzyme as replacement labels for radioisotopes enabled much of the sensitivity and specificity of radioimmunoassay (RIA) to be retained but without the inherent disadvantage of high capital cost, potential health hazard, and short shelf life of the labeled reactants.
Production of Monoclonal Antibodies to Phytohormones and Its Application
Korean Journal of Crop Science, volume 34, issue s01, 1989, Pages 48~54
An immunoassay techniques using monoclonal antibodies have been developed of the quantitative analysis of endogenous hormones in plants. In order to be useful for immunoassay, both a high degree of specificity and a high affinity are required. A system is described for production of hybridomas which secrete antibodies against the plant hormone. Using the system we were able to produce hybridmas with the desired antibody specificity by cell fusion and culture method. For a number of obvious reasons, monoclonal antibodies(mAb) were superior to polyclonal antibodies.