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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Crop Science
Editor in Chief :
Volume & Issues
Volume 47, Issue 6 - Dec 2002
Volume 47, Issue 5 - Dec 2002
Volume 47 - Dec 2002
Volume 47, Issue 4 - Sep 2002
Volume 47, Issue 3 - Jun 2002
Volume 47, Issue 2 - Jun 2002
Volume 47, Issue 1 - Mar 2002
Selecting the target year
Effects of Clipping on Growth and Yield in Sweet Potato
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 143~146
This study was undertaken to learn what ratio of clipping top was the most optimum for minimizing of the decrease of root yield of over 50g in sweetpotato at early cultivation. The test variety was "Shinyulmi" which was transplanted for early cultivation on April 18. The ratios of cripping top were 5, 10, 15, 20, and 25 percents. The date of clipping top was June 20 when the shoots were transplanted for double cropping. The total length of vine, the number of branches per plant, the number of tuberous roots over 50g and average root weight over 50g were not affected by clipping top. The ratio of root yield over 50g was lower over 20 percents of clipping top. The fresh weights per plant of top and bottom were similar as compared control with 15 percents of clipping top. In conclusion, the optimum ratio of clipping top was 15 percents for maximizing of the production of the shoots in sweetpotato for double cropping. cropping.
Effect of Harvesting Time and Additives on the Nutritive Values of Peanut Silage
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 147~150
To investigate the effects of Harvesting time and additives on the quality of peanut silage, the silage was mixed in combination with peanut leaf and stem, rice and barley straw, inoculant etc. Harvesting time at peanut growth stage was the best at 90 days after flowering with 30 branches,81 g of 100-seed weight, and 77% of shelling rate with low diseases in leaf and stem. By delay of harvest, diseases and lodging were increased. The yield of forage was the highest at 90 days after flowering as 52 t/ha, and seed yield was at 110 days as 3.72 t/ha. The yields between forage yield and seed yield (
) were correlated positively. The value of silage as a forage came over 90 days after flowering in combination with peanut leaf+stem+vice straw+inoculant treatment. The treatment was increased dry matter (DM) yield and neutral detergent fiber (NDF), the coarse protein (CP), and total digestible nutrient (TDN) content of silage in comparing with control (peanut leaf+stem). The pH at the organic matter contents of silage in combination of peanut leaf+stem+rice straw+lactic acid treatment was 5.04 to 5.10, the content of butyric and lactic acid were 3.12 to 4.64%, 2.07 to 7.34%, respectively.y.
Heritability Estimates of Sink and Source Characters by
Correlation in Rice
Hak-Soo SUH ;
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 151~156
The heritability of sink-source characters was estimated by regression coefficient between F
in two crosses of IR 66738-118-1-2/Gayabyeo and the Ilpumbyeo/YR 15965 Acp 33. In order to get the some basic information for breeding of high yielding rice, genetic resources of new plant type with low tiller and heavy panicle were used. Most of the sink and source characters in Ilpumbyeo/YR 15965 Acp 33 cross showed high heritability over than 0.224. But the specific leaf area and sink-source ratio displayed low heritability being 0.009 and 0.013, respectively. Heritability of all sink and source characters in IR 66738-118-1-2/Gayabyeo cross ranged from 0.115 to 0.247. Correlation coefficient between yield and yield components in both combination were in the ranged between 0.001 and 0.247. But correlations among the yield components were not significant. Correlations between the yield and sink-source characters in both combinations were also highly significant. Particularly, correlation between the grain tiling ratio and most of the sink-source characters in IR 66738-118-1-2/Gayabyeo cross were not significant. In Ilpumbyeo/YR 15965 Acp 33 cross, correlations between grain filling ratio and source characters were positively significant. but sink characters (sink capacity and sink-source ratio, etc) were negatively significant.
Comparison of Rhizome Harvesting Methods Saururus chinensis
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 157~160
This study was carried out to the harvesting efficiency of equipment used fer harvesting of rhizomes of Saururus chinensis Bail. Labor-saving efficiency showed 60-67% enhancement by using tillage operations with power tiller, digger attached to power tiller, and digger attached to tractor compared with manual harvest. Loss percentage of rhizomes by harvesting equipments was 2.2-8.8% lower than by manual harvest. Fresh rhizome yield and income index were increased 3-10% and 13-27% respectively by harvesting equipments, showing the highest yield and index in digger attached to tractor, Thus, digger attached to tractor showed the most effective harvest equipment for Saururus chinensis rhizome.
Comparison of Inorganic Elements and Epidermis Structures in Healthy and Rusty Ginseng
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 161~166
The ultrastructural characteristics of epidermis isolated from healthy and rusty ginseng roots(Panax ginseng) were observed by scanning electron microscopy (SEM), and the distribution profiles of inorganic elements were also examined by energy dispersive X-ray analysis (EDX). The epidermis of rusty ginseng was thick and cell walls were also severely disrupted whereas the epidermal image of healthy ginseng showed relatively thin, clear and rectangular structure. A high amount of rod-shaped bacteria was found in rusty ginseng and cells near bacteria were degraded. The bacterial density in epidermis of rusty ginseng was ranged from 2.9
CFU/g fresh weight, while that of healthy ones was from 4.7
CFU/g. Artificial inoculation of bacteria isolated from rusty ginseng induced similar symptom like rusty ginseng. The mineral contents inculding Al, Si and Fe were Higher in the epidermis of rusty ginseng, but K content was lower compared to healthy ones.
Effect of Continuous Cultivation Years on Soil Properties, Weed Occurrence, and Rice Yield in No-tillage Machine Transplanting and Direct Dry-seeding Culture of Rice
Jae Kil Lee ;
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 167~173
This experiment was conducted to figure out the change of soil physical properties, rice growth and yield with the years of continuous cultivation in direct dry-seeding and no-tillage machine transplanting. Experiments were conducted at NHAES(National Honam Agricultural Experiment Station, RDA, Iksan, Cheon Buk Province, South Korea) with a rice variety "Dongjinbyeo" from 1995 to 2000. In no-tillage machine transplanting cultivation, organic matter in soil was higher than that on direct dry-seeding and was significantly high in topsoil. Problematic weed species were E. crus-galli B., A. keisak H., and L. japonica M. Plant height and tiller number m-2 were higher in common-tillage during the total growth duration. The highest weedy rice occurrence of 27.5% was observed in live years' continuous direct dry-seeding and followed by 6.2%, in four years', and 3.7%, in three years'. The highest yield reduction of 38% was observed in five years' continuous direct dry-seeding. The reduction may resulted from the competition between weedy rice and cultivated rice.
Effects of Abscisic acid on Ozone Injury in Rice
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 174~185
This study was carried out to investigate the effects or abscisic acid (ABA), three concentrations, on growth, activities of antioxidant-related enzymes and grain yield of two rice plants (Oryza sativa L. cv. Hwamyungbyeo and cv. Namchunbyeo) exposed to ozone (0.150 ppm) for 6 hours a day for 30 days. The leaf chlorop0hyll contents, plant height, and tillering numbers were not shown significant difference. But ABA 10
M treatment affects to growth slightly. In all concentrations of ABA, superoxide dismutase (SOB) activities were increased at tillering stage of Namchunbyeo which was exposed to ozone. It is considered that the optimum concentration of ABA is 10
M for minimizing loss of grain yield loss.
Rooting and Acclimatization of Shoots Harvested from Bioreactor Culture in Rehmania glutinosa
Young-Am Chae ;
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 186~188
This experiment was carried out to know the effect of media and agar concentrations, aeration and growth regulators on rooting and acclimatization of the shoots harvested from bioreactor culture in Rehmannia glutinosa. Half MS media with 1.2% agar improved rooting and acclimatization of shoots. Shoots were effectively acclimatized and rooted well in case of aeration by using membrane filtered vessels. Shoots acclimatized in vessel with membrane Inter were healthier and had higher ex vitro survival rate than those without membrane Inter on plug tray. Addition of paclobutrazol 0.3-0.4 mg/L, to acclimatization media enhanced shoots growth and root development.
Comparison of Characteristics Related with Soybean Sprouts between Glycine max and G. soja
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 189~195
Soybean sprout trails were evaluated for soybeans for sprout (Glycine max) and wild soybeans (Glycine soja). Soybeans for sprout are characterized with small seed size of 10-14 grams per 100 seeds and are traditionally used only for producing sprouts. No difference was observed in hypocotyl length and sprout yield between two types of soybean. (However total length, hypocotyl hypocotyl, body weight, and the rate of good quality were greater in soybeans for sprout.) Number of lateral roots and root length were greater in wild soybeans. Contents of crude oil, oleic acid, and sucrose were higher in soybean for sprout, while that of protein and linoleic acid were higher in wild soybeans. There was no difference in sprout isoflavone content. Out of 17 amino acids analyzed, 10 amino acids such as aspartic acid, lysine, and arginine. etc. showed significantly Higher content in wild soybeans. Hypocotyl color of sprouts estimated by color meter based on the degree of L(brightness), a(red), and b(yellow) values were better in wild soybeans than soybean for sprout.
Effects of Planting Date and Accelerated Aging on Seed Germination-related Traits of Lipoxygenase-Lacking Soybean
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 196~200
Lower field germination was observed in lipoxygenase- lacking soybean genotypes. This study was performed to understand seed-related traits after accelerated aging in response to planting date of soybeans. Two soybean cultivars, Jinpumkong (null lipoxygenase-2,3) and Jinpumkong 2 (lacking lipoxygenase-1, 2, 3) were evaluated for germination, and were compared with the cultivar, Taekwangkong containing lipoxygenase-1,2,3 isozymes. Greater seed coat cracking was shown in Jinpumkong and Jinpumkong 2 than Taekwangkong. Regardless of soybean genotypes, earlier planting resulted in greater seed coat cracking. After accelerated aging, seed fracturability and hardness of Jinpumkong and Jinpumkong 2 were lower than those of Taekwangkong. There was significant difference in germination percentage among soybean genotypes in response to planting date. Seeds obtained from late planting showed better germination ability. Accelerated aging resulted in lower germination percentages of Jinpumkong and Jinpumkong 2 than that of Taekwangkong, and showed higher electric conductivity in Jinpumkong and Jinpumkong 2 than Taekwangkong. After accelerated aging at 4
for thirty five days, germination percentages of Jinpumkong and Jinpumkong 2 were lower than that of Taekwangkong. Electric conductivity was increased continually as accelerated aging time became longer, and Jinpumkong and Jinpumkong 2 showed higher electric conductivity than Taekwangkong. Even though there were significant genotype differences in seed traits, further studies are needed to determine whether seed lipoxygenase ability is associated with germination ability.
Effect of Storage Method on Seed Chemical and Germination Traits in Barley
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 201~205
This study was carried out to investigate storability and develop suitable storage method for barley. Rough and milled barley such as Olbori(covered barley), Ssalbori(naked barley) and Chalssalbori (waxy naked barley) were packaged in 3P bag and OPP film bag, respectively, and stored at ambient and low temperature warehouse conditions. The weight loss was higher in milled barley than that of rough barley during barley storage. Acid value, one of the most important factor for grain quality evaluation, was increased from 4.9-9.0 mg KOH/100g to 6.5-19.9mg KOH/100g in milled barley at 16 months after storage. Reducing sugar content was increased while germination and water absorption rates were decreased during barley storage period. In terms of penal taste of boiled barley, rough barley could be stored safely more than one year regardless of storage methods where-ase milled barley stored in ambient temperature ware-house produced nasty flavor at 8 to 10 months after storage.
Esterase Isozyme Patterns of Rice-off Ope Groups Classified by Grain Characteristics
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 206~210
This study was carried out to investigate the genetic segregations and characteristics of off-type rice plants collected in Korea which were classified into seven groups based on grain characteristics. In the analysis of esterase electrophoresis, the long-grain red group was classified as 1 and 3 esterase isozyme zymogram(EIZ), the long-grain normal group was classified as 1, 3 and 7 EIZ. The extremely late sterility group was segregated variously as 1, 2, 1+2, 5, 6, 5+6, 7,8 ,7+8 and 12 EIZ. The long-grain red rice lines with 1 EIZ had a longer culm length and a lower length/width ratio to brown rice than the long-grain red rice lines with 3 EIZ. The long-grain normal rice lines with 3 EIZ had a longer culm length, shorter panicle length, greater number of tillers, lower length/width ratio of brown rice, and fewer number of grains per panicle than did the long-grain red rice lines with 1 or 7 EIZ.
Optimum Harvesting Time Based on Growth Characteristics of Four-year Ginseng
Kwang-He Kang ;
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 211~215
This study was carried out to find out the optimum harvesting time on the basis of physiological characteristics, yield and commercial value in four years old ginseng. For this purpose, the changes of agronomic growth characteristics of the aerial and underground parts with different growing stages were examined with fourth year ginseng. The leaf growth was dramatically increased from the mid-April (shooting stage) to the mid-May (flowering stage), and the growth was nearly completed by the mid-May, but the stem growth continued by the mid-August. The weight of fresh root decreased from the April (20.7g) to the June (18.2g), but increased from the June to the October(45. 1g). The yield of fresh root per "Kan" was 1.5 kg, 1.2kg and 1.3kg at the April, the May and the June, respectively, thereafter continually increased to 2.9 kg of the October, which showing the maximum yield. In conclusion, the ginseng root was generally harvested from the September to the October at farm-house, but it had better be harvested after the mid-October rather than from September to October considering the weight of dry root and the yield of fresh ginseng.h ginseng.
Ginsenoside Concentration and Chemical Component as Affected by Harvestin Time of Four-Year Ginseng Root
Kwang-He Kang ;
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 216~220
This study was conducted to know the change pattern of chemical components, especially proximate constituents, saponin and free sugars in roots of 4-year ginseng according to different harvesting time, and to find out the optimum harvesting time on the basis of chemical components in fourth year ginseng root. The crude protein content was 20.77% of the highest on the April (shootinzg stage),13.13% of the lowest on the June among all growing stages, and was constant at 17% after the August. But crude oil and fiber contents showed the highest value on the May (flowering stage). The content of total sugars which was the largest constituent among the chemical components in ginseng root was in the range of 60~7(1% during the all growing stages. It showed the highest value of 7l% on the June, but the lowest of 60% on the May. The total free sugar contents was the highest on the April (20.40%), but the lowest on the May (11.89%). The change pattern of sucrose contents (10.96-19.60%) was same as the total sugars contents (11.89-20.40%), on the other hand, the contents of glucose and maltose were not changed significantly during all harvesting times. The contents of crude saponins and total ginsenosides had the value of 7.60% and 4.09% on the May, respectively. That was statistically significant, but the other harvesting times were not. Therefore, on the basis of the only chemical characteristics in the four year-old ginseng root, we suggest that the harvesting on the May at flowering stage was most proper time.
Effect of Latex Coated Urea on Growth and Yield in Rige Direct Dry Seeding for Water-saving Rice Culture
Jae Kil Lee ; Moon-Hee Lee ;
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 221~225
This study was conducted to identify the effects of slow release fertilizer (LCU) on ridge direct seeding on dry paddy of rice as an irrigation water-saying cultural system. During 1999-2000, a series of experiments was carried out at field (Chonbuk series) of the National Honam Agricultural Experiment Station, RDA using Dongjinbyeo. Plant height and culm length during the total growth duration were the longest and panicle umber per
was the highest in 100% LCU application rate. Leaf area index and top dry weight were the highest in the 100% LCU application rate. They increased as more LCU was applied. Nitrogen uptake was the highest in 100% LCU application, and N use efficiency was the highest in LCU 60% + FP (fertilization at panicle formation stage) 20% application. Milled rice yield was 7% higher in 100% LCU application rate than that of conventional N application. Ripened grain rate and 1,000-grain weight of brown rice did not differ, but panicle number per
and grain number per m2 were the highest at 100% LCU application.
Field Lodging Degree of Rice Varieties according to Nitrogen Application Rate
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 226~235
This experiment was to evaluate lodging characteristics of 35 recommended rice varieties of Kyonggi-do at normal (110 kg N/ha) and hen (220 kg N/ha) nitrogen application rates during 1999 to 2000. At heavy N fertilization rate, varieties that were highly resistant to lodging were Obongbyeo, Daejinbyeo, Naepoongbyeo, Nonganbyeo and Ilpumbyeo. Fourteen varieties including Daeanbyeo were moderately resistant and 11 varieties including Jinbubyeo were susceptible to lodging. The lodging-related characters which showed the highest effect on lodging at lodging degree of 9 were culm length and height of center gravity, culm length at lodging degree of 5, and culm length and the third internode length at lodging degree of 3 or below. The difference in rice yield between normal and heavy nitrogen fertilization rate was within 5% in early-maturing varieties but there was great yield difference in mid- and late-maturing varieties depending on the degree of lodging. Consequently, stable rice yield could be obtained by selecting lodging-resistant varieties and lowering fertilization rate, specially in the paddy field where lodging occurrence is common.
Saponin Composition of Liriope platyphylla and Ophiopogon japonicus
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 236~239
Saponin is one of major conponents in oriental medicine, which':s present in Ophiopogoneae. Saponin composition on ethyl acetate fractions from the butanolic extracts of two species, Liriope platyphylla and Ophiopogon japonicus was measured using high performance liquid chromatography (HPLC). HPLC analysis revealed that spicatoside A and B were identified in the tuber of L. platyphylla. However, spicatoside A was not identified in the tuber of O. japonicus. Related to that, it may be possible to discriminate between Liriopis and Ophiopogonis Tuber using HPLC analysis.
Seed Protein Content and Fatty Acid Composition of Black Seeded Soybeans Collected From Southwestern Islands
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 240~243
A total of 135 black seeded soybeans genotypes were collected in 45 island locations from January to May 2001. Seeds of 135 genotypes collected were analyzed for crude protein and fatty acid compositions. The crude protein content was averaged to be 40.75%, and was ranged from 34.70% to 44.20%. The average palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid content were 11.41%, 3.93%, 22.75%, 53.55%, and 8.35%, and the ranges of those were 9.00% to 14.40%, 2.90% to 5.00%, 22.75% to 26.50%, 50.30% to 57.20% and 6.7% to 11.20%, respectively. Heritabilities of palmitic acid, oleic acid, linoleic acid, and crude protein were higher, but that of stearic acid and linolenic acid were relatively lower, Crude protein content was correlated positively with oleic acid content, whereas it was correlated negatively with linoleic acid, palmitic acid, stearic acid, and linolenic acid contents.
Water Uptake and Germination of Soybean Seed as Affected by Soaking Condition
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 244~249
Varietal difference in seed shape and size, water absorption rate, and soybean sprouts was compared. In general, soybean seeds of yellow seed-coat and spherical shape with 100 seeds of 9.0
2g were evaluated as the best ones; Pungsannamulkong out of tested varieties was considered to be the best one in these respect. Varietal difference in water absorption yale depending on the soaking duration and temperature was recognized; Jungeri and Jillin 3 showed higher water absorption rate at higher temperature but it was completely vice versa for Pungsannamulkong. It took about 15 hours(soaking at 2
) for seed shape of size to grow to 10mm in length. Maximum enlargement in thickness of soybean seeds was made right after the completion of repeated soaking-drying treatment but three hours and nine hours soaking were needed for one time-soaking and non-soaking treatment, respectively. Varietal difference in germination rate was recognized between one hour's soaking at 2
and two hour's soaking at 15
The growth rate for Jillin 3 was excellent at all soaking methods and temperatures while three hours of soaking was the best for Jungeril and Pungsannamulkong, regardless of soaking temperatures. The growth of hypocotyl length showed somewhat faster in repeated soaking-drying than one time-soaking and non-soaking while the increase of hypocotyl thicknes was better in one-time soaking than repeated soaking-drying.
Post Harvest Management of Bulk-Harvested Barley Using Rice and Barley Processing Complex
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 250~253
The post harvest treatment of barley had many hard work steps, such as drying, cleaning, and packing. This is a reason why farmer doesn't like to cultivate barley. This study was conducted to investigate the optimum post harvest management of bulk-harvested barley using rice processing complex (RPC). Bulk-harvested barley was stored to 61.1% after 6pm at RPC. Grain moisture contents of bulk-harvested barley differed from storing date, farmer household, and field. Required dry hours were different with grain moistures contents. The average dry hour was 9.5 hours per 10a and dry rate was 0.89%. The proportion of impurity removed by coarse cleaning differed from grain moisture contents, as higher grain moisture content made impurity rate increase up to 38.9%. Cost of drying of bulk-harvested barley was 50won per ㎏ at above 24% of grain moisture contents, and the average dry cost was 41.25 won/㎏ and 14,400won/10a. The 62% of barley treated was stored in indoor-grain bin, and the others packed in 500 ㎏-poly con bag were stored in warehouse insulated.
Effect of Harvest Time on Yield and Quality of Rice
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 254~258
The characteristics of yield and quality in 3 rice varieties according to harvest time of 40, 50, 60 and 70days after heading(DAH) was investigated to obtain basic information for the production of high quality rice. The protein content of milled rice increased significantly as increase the ripening period from 40 to 70DAH. The palatability value measured by rice taster was the highest in ripening period of 40DAH and decreased with increase of ripening period. The optimum time for harvest in terms of both rice yield and quality was 4050DAH in Daejinbyeo, and 4060DAH in Seojinbyeo and Chucheongbyeo, however, it was considered to be 4050DAH only for rice quality. The palatability value measured by rice taster showed a highly negative correlation with protein content of milled rice(1=-0.94
) and cumulative ripening temperature(r=-0.79
Relationship between Yield and Quality of Rice Varieties Grown in Reclaimed Saline Paddy Field
Je-Cheon Chae ;
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 259~262
Relationship between yield and palatability value measured by vice taster among 9 rice varieties was investigated to obtain basic information for production of rice with high quality in reclaimed paddy field. The rice yield showed a highly negative correlation with palatability value of milled rice (r=-0.49
). The varieties of high palatability value tend to show low yield. The protein content of milled rice grown in reclaimed paddy field was higher than 7.5% mainly due to heavy fertilization of nitrogen and irrigation of eutrophicated water, The rice varieties such as Daeanbyeo, Jinpumbyeo and Sindongjinbyeo were recommendable only for higher yield, but in view of rice quality, Kwanganbyeo, Surabyeo and Nampyoengbyeo were more recommendable because of their lower protein content and higher palatability value.e.
Varietal Difference in Relationship between SPAD Value and Chlorophyll and Nitrogen Concentration in Rice Leaf
Korean Journal of Crop Science, volume 47, issue 3, 2002, Pages 263~267
This experiment was conducted to find out the varietal differences in the relationships of chlorophyll and nitrogen concentration with leaf greenness value (SPAD value). We used two rice varieties with different leaf color type: Hwaseongbyeo has pale green leaves, and Gwanganbyeo has dark green leaves. SPAD value was correlated with chlorophyll a concentration significantly in both varieties, but not with chlorophyll b in Hwaseongbyeo. The correlation coefficients between SPAD value and nitrogen concentration of rice flag leaves were highly significant. However, the regression analysis between SPAD value and nitrogen concentration of rice flag leaves showed different relation among varieties. The slope of the regression line in Gwanganbyeo was steeper than that in Hwaseongbyeo. In conclusion, SPAD value can be applied to estimate the chlorophyll a concentration without consideration of varietal differences. Chlorophyll b and nitrogen concentration in the leaf were regressed to the SPAD values differently with varieties. This implies that the SPAD value can not be used directly to estimate chlorophyll b and nitrogen concentration without consideration of varietal differences.