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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Crop Science
Journal Basic Information
Journal DOI :
The Korean Society of Crop Science
Editor in Chief :
Volume & Issues
Volume 49, Issue 6 - Dec 2004
Volume 49, Issue 5 - Dec 2004
Volume 49, Issue spc1 - Oct 2004
Volume 49, Issue 4 - Sep 2004
Volume 49, Issue 3 - Jun 2004
Volume 49, Issue 2 - Jun 2004
Volume 49, Issue 1 - Mar 2004
Selecting the target year
Varietal Difference of Transplanted Rice Seedling Growth in Response to Salinity
Lim Jae-Joong ; Kim Choong-Soo ; Cho Jin-Woong ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 271~278
This study was conducted to obtain basic information of growth, chlorophyll, and
content of rice (Oryza sativa L.) seedlings after transplanting in different NaCl conditions. Plants grown in pots for 8 days after germination were grown for 10 days after transplanting in 50 and 100 mM NaCl concentrations. At higher NaCl concentration, plant height, root length, dry weight and chlorophyll content were reduced with NaCl stress. Among rice cultivars, the shoot dry weight of Gancheokbyeo, Janganbyeo and Hwasungbyeo, and the root dry weight of Janganbyeo, Gancheokbyeo and Juanbyeo showed relatively low reduction compared to the other rice cultivars at 100 mM NaCl stress. The
content in seedling rapidly increased with the increase of NaCl concentration but
contents decreased. There was a significant relationship between
content and shoot and root dry weight after transplanting rice seedlings to saline conditions. The shoot and root dry weight showed highly negative relationship with the
ratio in saline conditions.
Comparison of Two Soybean Cultivars in Dry Matter Production and Ecophysiological Characteristics
Cho Jin-Woong ; Lee Jung-Joon ; Kim Choong-Soo ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 279~283
This experiment was carried out at paddy field (commercial silty loam soil) in the southwestern Korea. Pungsannamulkong, a determinate growth habit, was a relatively high yielding and late maturing cultivar, and Hannamkong, a semi determinate growth habit, was a relatively low yielding and early maturing cultivar. Seeds were sowed at two plants and with a planting density of
on May 26, 2003. Fertilizer was applied prior to planting at a rate of 3.0-3.0-3.4g
by all basal fertilizations. Experimental design was a randomized complete block with three replications. Seed yield was higher in Pungsannamulkong by 362g per
than in Hannamkong of 260g per
Also, the number of pod, number of seed, and number of seed per pod were greater in Pungsannamulkong than in Hannamkong. The number of leaves per
showed similar with two soybean cultivars up to August 24 but thereafter it decreased in Hannamkong. The leaf area up to August 4 increased in Hannamkong higher than in Pungsannamulkong, but after that time, Pungsannamulkong had greater leaf area than Hannamkong. The shoot and leaf dry matter of two soybean cultivars from June 23 to August 4 were similar but thereafter, Pungsannamulkong had a significantly greater than Hannamkong. Crop growth rate (CGR), relative growth rate (RGR) and net assimilate rate (NAR) for Punsannamulkong were relatively higher than Hannamkong but leaf area ratio (LAR) and specific leaf weight (SLW) showed higher in Hannamkong. Most of leaves distributed in the ranges of 80-90cm and 60-70cm from the soil surface in Punsannamulkong and Hannamkong, respectively. Pods of Punsannamulkong ranged 10-80cm from the soil surface and most of pods were distributed at 40-50cm. Photosynthetic rate at the flowering stage showed a significant difference between cultivars in the upper most leaf position. There was no significant difference of the photosynthetic rate at
leaf at the flowering stage, and the uppermost and 7th leaf position at the seed development stage between two soybean cultivars.
Morphological Difference of Rice Seedling Grown under Different Dissolved Oxygen Conditions
Won Jong Gun ; Choi Jang Soo ; Ahn Deck Jong ; Lee Seung Phil ; Lee Sang Chul ; Yoshida Tomohiko ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 284~288
The response of dissolved oxygen (DO) concentrations caused significant change in root number, root length, coleoptile length, shoot length and leaf age of seedlings. The genotypic difference in the effect of DO also highly significant (P<0.01) for all of the seedling traits. The number and length of root were extremely inhibited at the condition of
DO concentration. While the coleoptile elongated markedly in the lowest DO concentrations, the shoot did not develop. The root growth was improved slightly at the
, however, there were no difference among genotypes at these two low DO concentrations. As the DO concentration become higher, the growth of root and shoot was improved remarkably. Root number, root length and shoot length was significantly different between
in DO rich and normal conditions, the development of those traits were apparently accelerated in high water temperature, however those traits of seedlings in DO deficiency were not different between the two temperatures except for shoot length. On the other hand the coleoptile length was not affected by the stagnant water temperature; it was stimulated by the low DO concentration. The competition of DO was greater as the seedling density was increased in the stagnant water, therefore the seedlings grown under high density have long and white coleoptiles, and the growth of roots and shoots was retarded severely.
Activity and Isozyme Profile of Antioxidative Enzymes at Booting Stage of Rice Treated with Cold Water
Kim Ki-Young ; Kim Bo-Kyeong ; Shin Mun-Sik ; Choung Jin-Il ; Ko Jae-Kweon ; Kim Jung-Kon ; Lim Jung-Hyun ; Yun Song-Joon ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 289~294
This study was carried out to investigate the antioxidative enzymes and isozymes between chilling-tolerant and -susceptible varieties at the booting stage under cold water stress
in japonica rice. Total SOD, CAT, POX, and GR activities on the basis of protein were found to be important factors to defend cold water stress. Especially, SOD and CAT activities showed distinctive differences between chilling-tolerant and -susceptible varieties. Chilling-tolerant varieties were higher than chilling-susceptible varieties for SOD and CAT activities. One of eight isozyme bands for SOD was a inducible isoform. Three isozymes for CAT and one isozyme for POX were closely correlated with defense to cold water stress. Total GR activities except Stejaree 45 on the basis fresh weight and POX were increased by cold water stress, but there was no difference between chilling-tolerant and -susceptible varieties.
Classification of Korean Green Tea Products Based on Chemical Components
Chun Jong Un ; Choi Jeong ; Lim Keun-Cheol ; Kim Yong-Gul ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 295~299
The prices of domestic green tea products are relatively expensive and price differences within products of the same levels of quality are various. Also, there is no basic criteria on evaluation of green tea quality. To group 43 commercial green tea products into several parts by the principal component and cluster analyses, this work was done by use of 8 chemical constituents which were analyzed by NIR system. The principal component and cluster analyses revealed 8 groups. The first group included 16 products that had lower free amino acid and theanine contents. The second group included 5 products having higher free amino acid and theanine contents, but lower ash contents. The third group included 13 products showing medium values of 8 constituents. The IV group included 4 products having higher contents of moisture, free amino acids, and theanine. The V group included 1 product showing higher moisture but lower catechins contents. The VI group included 2 products that had higher moisture and catechins contents, but lower free amino acid and theanine contents. The VII group had higher moisture and catechins contents. The VIII group had higher ash and vitamin C contents. The free amino acid contents which were the most important in flavor evaluation of green tea quality did highly positively correlate with the contents of total nitrogen
, but negatively with the contents of ash
. The catechins used as for functional ingredients did correlate with contents of caffeine(+) and vitamin C(-), respectively.
Effects of Gibberellic Acid and Gibberellin Biosynthesis Retardants on Ethylene Production, Batatasins, and Free Sugars in Dormant Tubers of Chinese Yam
Kim Sang-Kuk ; Lee Sang-Chul ; Kim Kil-Ung ; Choo Yeon Sik ; Kim Hak Yoon ; Lee In-Jung ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 300~304
Gibberellic acid did not affect ethylene production, whereas gibberellin biosynthesis inhibitors triggered ethylene production in dormant tubers. Gibberellic acid did not induce sprouting of dormant tubers, however, treatment of gibberellin biosynthesis retardants enhanced sprouting rates. Sprouting rate in ancymidol-treated tubers was highest among gibberellin biosynthesis retardants. Sprouting rate of tubers treated with ancymidol increased to
. Batatasin-III content in
treated tuber was increased in the highest concentration
. Tubers treated with mepiquat chloride, Batatasin-I was increased steadily, but contents of Batatasin-III and V showed dramatic decrease at the
concentration. This infers that gibberellin biosynthesis retardants play key roles in promoting breaking dormancy on dormant tubers of Chinese yam.
Effects of Vermicompost on Growth of Fall-Cropping Potato in Volcanic Ash Soil
Kang Bong Kyoon ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 305~308
Recently, with increasing concern for sustainable agriculture and safe agricultural products, organic farming has become widely adapted as an alternative to conventional farming. This study was conducted to investigate the effects of earthworm casts (EWC) with
organic compost on the growth and yield characteristics of fall-cropping potato (Solanum tuberosum L. cv. Dejima) in Jeju. The treatments consisted of seven plots: 2-, 4-, 6-, 8-, and 10-ton
EWC-treated plots, 1.2-ton
(CEP)-treated plot as conventional practices, and a control plot. The plant heights were greater in the plots where EWC and CFP were applied than in the control plot. Tuber diameter, number of stems per plant, and chlorophyll level tended to increase in the plots where 8-10 tons
of EWC were applied. The application of CFP and EWC showed an increment in the average tuber weight per plant, but there was minimal significant difference. The application of 8-10 tons
of EWC resulted in an increase in the total tuber yield (21.61-21.87 tons
) as compared to the other plots. The highest yield of market able tubers was
of the total yield from the 10-ton
EWC-treated plot. Consequently, with regard to the growth and yield characteristics of fall-cropping potato, the effects of EWC application were more favorable than the effects of the application of a chemical fertilizer.
Seed Quality of Soybean Produced from Upland and Drained-Paddy Field
Kim Sun-Lim ; Park Keum-Yong ; Lee Yeong-Ho ; Ryu Yong-Hwan ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 309~315
This study was carried out to evaluate the seed quality of soybeans produced from upland and drained-paddy fields. Soybeans from drained-paddy field showed significantly higher in the 100 seeds weight and greater in the size of seed length than those from upland fields. However, there are no significant differences in seed width and thickness between upland and paddy fields. In case of Hunter's color value, the lightness (L) was significantly higher in the upland soybeans, but the a (redness) and b (yellowness) values were higher in the drained-paddy field soybeans. Seed appearance of drained-paddy field was poor than that of upland field. Soybeans produced from the drained-paddy field showed higher protein content, whereas, lipid and ash contents were higher in the upland field. Soybeans from upland field had lower contents of total amino acids compared to drained-paddy fields. No statistical differences were found in palmitic, linoleic, and linolenic, but stearic, oleic, saturated fatty acids (SFA), and unsaturated fatty acids (USFA) showed significant differences between soybean seeds from upland and drained-paddy fields. Genistein content was higher in the drained-paddy fields, while daidzein and glycitein contents were higher in the upland fields. This result suggested that the soil condition of drained-paddy field is more favorable to synthesis genistein than daidzein and glycitein.
An Ultrastructural Investigation of Infection Threads in Sesbania rostrata Stem Nodules Induced by Sinorhizobium sp. Strain MUS10
Krishnan Hari B. ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 316~324
Sinorhizobium sp. strain MUS10 forms nitrogen-fixing stem nodules on Sesbania rostrata, a tropical green manure crop. In this study, the ultrastructural events associated with the formation of stem nodules were investigated. Sinorhizobium sp. strain MUS10 entered the host tissue through cracks created by the emerging adventitious root primordia and multiplied within the intercellular spaces. During early phases of infection, host cells adjacent to invading bacteria revealed cellular damage that is typical of hypersensitive reactions, while the cells at the inner cortex exhibited meristematic activity. Infection threads were numerous in S-day-old nodules and often were associated with the host cell wall. In several cases, more than one infection thread was found in individual cells. The junction at which the host cell walls converged was often enlarged due to fusion of intracellular branches of infection threads resulting in large infection pockets. The infection threads were made up of a homogeneous, amorphous matrix that enclosed the bacteria. Several finger-like projections were seen radiating from these enlarged infection threads and were delineated from the host cytoplasm by the plasma membrane. As in Azorhizobium caulinodans induced root nodules, the release of Sinorhizobia from the infection threads into the plant cells appears to be mediated by 'infection droplets'. A 15-dayold Sesbania stem nodule revealed typical ultrastructure features of a determinate nodule, containing several bacterioids within symbiosomes.
Effects of Planting Dates on Growth and Yield of Soybean Cultivated in Drained-Paddy Field
Cho Jin-Woong ; Lee Jung-Joon ; Kim Choong-Soo ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 325~330
This study was carried out to determine adequate planting date, to compare the growth characteristics between early and late maturing cultivars, and to provide the data for the cultivation techniques of soybean [Glycine max (L.) Merr.] in double cropping system with winter crops on paddy field in Korea. Cultivars were planted on 26 May, 16 June, and 7 July with a planting density of
(planting spacing). Seed yield of soybean planted on June 16 and July 7 was approximately
, respectively, less than that of conventional planting date of May 26 in Pungsan-namulkong, and planted on June 16 and July 7 was about
, respectively, less then that of conventional planting date of May 26 in Hanamkong. The number of pods and seeds per plant decreased as planting date delayed. Seed weight increased in Pungsan-namulkong but decreased in Hannamkong as planting date delayed. The flowering date was late in delayed planting plots, but it was shorted for days from emergence to flowering and from emergence to maturity. The plant height of Hannamkong was greater than Pungsan-namulkong from the emergence to flowering stages, but in contrast, it was greater in Pungsan-namulkong than Hannamkong after flowering stage (50d after emergence) when it planted on May 26. There were no significant differences between two soybean cultivars at planting dates of June 16 and July 7. Leaf number, leaf area, and dry matter were also reduced by late planting, and Both of them were shown in high reduction at the later planting. There was a high significant difference at the flowering
and pod formation
stages between leaf dry matter and seed yield. Crop growth rate (CGR) was greater at
growth stages compared to
growth stages in two soybean cultivars and the greatest CGR was obtained at planting date of May 26 in two soybean cultivars except for R4-R5 growth stage in Pungsan-namulkong. There was a highly significant positive difference between the seed yield and the leaf area index (LAI) across R3 to R4 and R2 to R3 stages. The photosynthetic rate
of the uppermost leaf position had no significant difference among planting dates and between two soybean cultivars. However,
leaf position increased as the planting date delayed.
Changes of Chemical Components During Seed Development in Black Soybean (Glycine max L.)
Shim Sang In ; Kang Byeung Hoa ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 331~336
Changes in the level of metabolites in leaves and pods were examined with respect to the seed chemical composition in black soybean. There was no further increase in pod length after 42 days after flowering (DAF). Pod weight, however, persistently increase until 73 DAF, thereafter the weight was slightly lowered. The seed storage protein, however, increased drastically as the increasing rate of pod weight was lessened at 61 DAF. The accumulation of seed storage proteins was occurred conspicuously as the increasing rate of pod weight was slowed down. The chlorophyll content both in leaves and pods was drastically decreased after 50 DAF. The beginning of drastic reduction in chlorophyll content was occurred concomitantly with the reduction of soluble protein content in leaves. The sugar content in leaves showed similar tendency with chlorophyll and soluble protein content. The starch level in leaves, however, showed different changing pattern during seed development. The starch content in leaves was increased persistently until 66 DAF, thereafter the content was decreased drastically to about
of maximal value at 66 DAF. Total phenolics content in leaves and the anthocyanins content in seeds were stable without noticeable increase until 66 DAF. The contents were increased dramatically after 66 DAF showing the synchronized pattern with the decrease in starch level in leaves. The levels of the selected metabolites in leaf and seed suggested that the accumulation of chemical components of black soybean seed is launched actively at 66 DAF. The profile of storage proteins was nearly completed at 61 DAF because there was no large difference in densitometric intensity among protein subunits after 61 DAF. In soybean, chemical maturation of seed begins around 61 to 66 DAF at which most metabolites in vegetative parts are decreased and remobilized into maturing seeds.
Morphological and Genetic Diversity of Korean Native and Introduced Safflower Germplasm
Shim Kang-Bo ; Bae Seok-Bok ; Lim Si-Kyu ; Suh Duck-Yong ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 337~341
Morphological and genetic diversity of thirty nine safflower germplasm were collected and evaluated by Principal Component Analysis (PCA) and Random Amplified Polymorphic DNA (RAPD) method. Stem length and seeding to flowering days of the safflower germplasm showed
days of variation respectively. USA originated germplasm showed higher oil content as
, but that of Japanese showed lower as
. PCA made three different cluster groups according to some agronomic characteristics of safflower. Korea originated germplasm showed similar cluster group with that of collected from USA in the PCA of stem length. But in the seeding to flowering days, it showed similar cluster pattern with that of collected from Japan rather than USA. In the experiment of RAPD analysis, total five primers showed polymorphism at the several chromosomal loci. Korea, China Japan and South Central Asia originated germplasm were differently classified with USA and South West Asia originated germplasm with lower similarity coefficient value (0.47). Most of Korea originated germplasm were grouped with South Central Asia originated germplasm with higher similarity coefficient value (0.74) conferring similar genetic background between both of them. China and Japan originated germplasm were dendrogramed with Korea originated germplasm at the 0.65 and 0.50 similarity coefficient values respectively. Some common results were expected from both of PCA and RAPD analysis, but lower genetic heritability caused by relative higher portion of environmental variance and environment by genotype interaction at the expression of those of agronomic characteristics made constraint to find any reliable results.
A Simple and Rapid Method to Isolate Low Molecular Weight Proteinase Inhibitors from Soybean
Krishnan Bari B. ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 342~348
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the
isopropanol extract of soybean(Glycine max [L.] Merr.) seed revealed two abundant proteins with molecular masses of 19 and 10 kDa. Amino acid analysis revealed that the isopropanol-extractable protein fraction was rich in cysteine. Two-dimensional gel electro-phoretic analysis indicated that the 19kDa and 10kDa proteins had pI of 4.2 and 4.0 respectively. Peptide mass fingerprints of trypsin digests of the two proteins obtained using matrix-assisted, laser desorption/ionization-time of flight (MALDI-TOF) mass spectroscopy revealed the 19kDa protein was Kunitz trypsin inhibitor and the 10kDa protein was Bowman-Birk proteinase inhibitor. When resolved under non-denaturing conditions, the isopropanol-extracted proteins inhibited trypsin and chymotrypsin activity. Results presented in this study demonstrate that isopropanol extraction of soybean seed could be used as a simple and rapid method to obtain a protein fraction enriched in Kunitz trypsin and Bowman-Birk proteinase inhibitors. Since proteinase inhibitors are rich in sulfur amino acids and are putative anticarcinogens, this rapid and inexpensive isolation procedure could facilitate efforts in nutrition and cancer research.
Improvement of Regeneration Efficiency from Mature Embryo and Leaf Base Segment in Korean Oat Genotypes
Kim Kyung-Hee ; Moon Jung-Hun ; Lee Sang-Kyu ; Lee Byung-Moo ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 349~353
Mature embryo and leaf base segment of Korean oat were used as materials in an experiment to check plant regeneration efficiency. MS media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and picloram were used for callus induction from mature embryos and leaf base segments. Three mg/l of 2,4D and 3 mg/l of picloram in callus induction medium showed high frequency for plant regeneration from mature embryos. Leaf base segments were transferred to callus induction medium and incubated at
in 16/8 hr light/dark cycle for 3 weeks. Callus induction from leaf base segments of Malgwiri showed high efficiency in medium containing 3 mg/l of 2,4-D and 1 mg/l of kinetin
. In case of Samhangwiri, the combinations of phytohormones did not show significant difference. Regeneration from leaf base segments showed high frequency in shoot medium containing 1 mg/l of antiauxin, tri-iodobenzoic acid (TIBA) and 1 mg/l of 6-benzyladenine (BA). Calli induced from leaf base segments of Samhangwiri and Malgwiri in media containing 3 mg/l of 2,4-D and 3 mg/l of picloram showed high regeneration frequency. It appears that the callus initiation medium may be an important factor for subsequent plant regeneration.
Cryopreservation of Zygotic Embryos of Herbaceous Peony (Paeonia lactiflora Pall.) by Encapsulation-Dehydration
Kim Hyun-Mi ; Shin Jong-Hee ; Sohn Jea-Keun ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 354~357
A simplified technique which cryoprotects zygotic embryos by encapsulation-dehydration was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest survival rate
was obtained from embryos treated by encapsulation-dehydration. The zygotic embryos were precultured on MS medium containing 0.3mg/L
for 1 day. The precultured embryos were encapsulated in
(w/v) alginate beads and immersed for 1 h in MS medium containing 2 M glycerol and 0.5 M sucrose. The encapsulated embryos were dehydrated for 5h by air drying prior to direct immersion in liquid nitrogen. This encapsulation-dehydration method appears to be a promising technique for germplasm cryopreservation of a herbaceous peony.
Development of AFLP and STS Markers Related to Stay Green Trait in Multi-Tillered Maize
Jang Cheol Seong ; Lee Hee Bong ; Seo Yong Weon ;
Korean Journal of Crop Science, volume 49, issue 4, 2004, Pages 358~362
In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a
homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a
homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.