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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
The Korean Society of Phycology
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Volume & Issues
Volume 12, Issue 3 - Sep 1997
Volume 12, Issue 4 - 00 1997
Volume 12, Issue 1 - 00 1997
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Silica-scaled Chrysophytes (Synurophyceae) in Several Reservoirs, Swamps and a Highland Pond from Changnyong County, Korea
Kim, Han Sun ;
ALGAE, volume 12, issue 1, 1997, Pages 1~1
Silica-scaled Chrysophybes (Synurophyceae)flora at 13 localities from central-south area of Korea were investigated using electron microscope. Twenty-seven taxa of Mallomonas and nine taxa of Synura were identified by transmission and scanning electron microscopy. Among these, M. insignis, M. lelymene, M. oviformis, M. parvula and M. torquata are reported for the first time in Korea and M. trummensis is new to Asia. A very rich flora (33 taxa) of Synurophyceae which amounts to ca. 81% of the Korean flora of these organisms known so far was found in the Maetan reservoir and a large number of species were found only in the mesotrophic waters. However, very few species were found in the highly polluted eutrophic waters and highland dystrophic water.
Growth of Phaeophyte (Fucus serratus) in Culture and Changes of the Amounts of Triacylglycerols and Fatty Acids
ALGAE, volume 12, issue 1, 1997, Pages 11~11
Focus serratus was cultivated for 13 weeks and-analyzed to its triacylglycerols and fatty acids. After a month of culture, the thalli were grown into 0.1 ㎝ per week. This period was corresponded with the lag phase on growth curve. The exponential phase occurred between 4th and 10th week of culture as the growth of thalli of 0.4 ㎝ and 2.1㎝ per week. The averages of amount of total lipids (TL) and triacylglycerols (TAG) in F. serratus were 8.4 ㎎·g^(-1)dry wt (0.80% of dry wt) and 1.9 ㎎·g^(-1)dry wt (0.2%), respectively. For the duration of culture, the amounts of TL and TAG tend to decrease from 16.3 to 4.3 ㎎·g^(-1)dry wt for TL and from 2.9 to 1.1 ㎎·g^(-1)dry wt for TAG. However, the dry weight of thalli was increased in culture (130 -$gt; 241 ㎎·g^(-1)fresh wt^(-1)). In TL and TAG, C18:1(oleic acid) was globally more abundant than C16:0 (palmitic acid) in culture. In TL, the amount of C16:0, C18:1 and C20:4 (arachidonic acid) were major. In TAG, the dominant fatty acids were C16:0, C18:1 and C18:2.
Characterization of UV-absorbing Compounds in Korean Macrophytes, Ulva pertusa Kjellman (Chlorophyta), Hizikia fusiformis (Harvey) Okamura (Phaeophyta), and Pterocladia capillacea (Gmelin) Bornet et Thuret (Rhodophyta)
Sin, Hyeon Ung ; Kim, Gwang Hun ;
ALGAE, volume 12, issue 1, 1997, Pages 17~17
UV-absorbing compounds were investigated in Korean macrophytes using, absorbance and fourth-derivative spectra. Pterocladia capillacea and Hizikia fus fusiformis each revealed a single absorbance maximum at 334 ㎚ while Ulva pertusa did not show any UV-absorbing compound absorbance maximum. Fourth-derivatives of P. capillacea and H. fusiformis revealed two predominant UV-absorbing maxima at 325 and 350 ㎚ and three absorbance maxima at 325, 338.5 and 356 ㎚. The ratios of absorbance maxima UV-absorbing compound to Chl. a were 1.28 for P. capillacea and 4.51 for H. fusiformis, respectively.
Succession Pattern of Intertidal Benthic Algal Communities in Cheju Island
Kim, Yeong Hwan ; Park, Seon Hong ;
ALGAE, volume 12, issue 1, 1997, Pages 23~23
Studies have been made of the recolonization and succession of benthic marine algae on cleared natural surfaces over a 24-mo period (October 1994 to October 1996) at intertidal zone of Shinchang, Cheju Island. Non-destructive method was employed monthly to measure cover as well as species composition. A total of 51 species of algae (3 green, 15 brown and 33 red algae) was found in the permanent quadrate. Numbers of marine algal species found in the permanent quadrate, on the whole, showed highest at the lower tidal zone among the tidal levels. Pioneer species found in the permanent quadrate within first three months since denudation were mostly perennial algae. Major species in the succession sere were Hizikia fusiformis in the upper and middle, and Ulva conglobata and Corallina pilulifera in the middle and lower tidal zones. Since annual algae found in the permanent quadrate were mostly ephemerals and also perennial algae occurred at the start of succession, it therefore seems likely that annual species do not play significant role in successional pattern in the intertidal zone. It was assumed that, in Cheju Island, the climax phase can be attained after one year since the substrata wee cleared.
Neurotoxin-producing Pseudonitzschia multiseries (Hasle) Hasle, in the Coastal Waters of Southern Korea Ⅱ. Production of Domoic Acid
Lee, Jin Hwan ; Baek, Jeong Hyeon ;
ALGAE, volume 12, issue 1, 1997, Pages 31~31
The marine diatom, Pseudonitzschia multiseries was known as a producer of domoic acid. This species was eaten by oysters, clams and blue mussels etc. from which damoic acid was accumulated and extracted. P. multiseries was abundant in the coastal waters of southern Korea, but little has been known concerning toxins in details. In order to determine the presence of domoic acid in the axenic strain of P. multiseries and to certify the occurrence of domoic acid in P. multiseries and Mytilus edulis, this study was carried out during the period from April 1993 to May 1995 in Jinhae Bay.A single done was isolated and cultured by means of f/2 medium and then cultured diatoms were analyzed at each growth stage along with reverse page HPLC. The aqueous extracts of M. edulis were analyzed with reverse phage HPLC. Domoic acid was also detected in P. multiseries, which has had relations with the food chain between this diatom and M. eduiis.Domoic acid of P. multiseries was increased abruptly during the stationary phase (7-15 day), and the domoic acid content per cell was about 1.03 pg/cell. Quantitative analysis of M. edulis yielded variable levels of domoic acid from 30.85-33.55 ㎎/㎏ in a cell density of 1.5-9.5 × 10^5 cells/ℓ. Toxicity of M. edulis was relatively high during the occurrence of blooms of P. multiseries. Domoic acid-producing P. multiseries was abundant in April and May when the water temperature is 14.8℃ to 19.0℃.
Induction of Pigmentation Mutants by Treatment of Monospore Germlings with NNG in Porphyra yezoensis Ueda (Bangiales, Rhodophyta)
Xing Hong Yan ; Yusho Aruga ;
ALGAE, volume 12, issue 1, 1997, Pages 39~39
Monospore germilings of Porphyra yezoensis Ueda (Bangiales, Rhodophyta) were toted with NNG (N-methyl-N'-nitro-N-nitrosoguanidine) to induce mutation. After the treatment were obtained spottedly variegated foliose thalli with small clusters of mutant cells of various colors. The foliose thalli were treated with sea snail gut enzyme to obtain single cells and protoplasts of different color, which were cultured and grew up to young single-color foliose thalli. The young thalli were individually cultured and they produced manospores, which grew up to foliose thalli of different color (3 reddish, 2 greenish, 1 orange and 1 golden-brownish mutants). From each of the mutant foliose thalli, carpospores were obtained to grow respective free-living conchocelis in culture. In vivo absorption spectra and pigment contents (chlorophyll a, phycoerythrin, and phycocyanin) of the mutants were compared with each other and with those of the wild type thallus.
A Simple Method for RNA Extraction from Marine Macroalgae
Kim, Sun Hui ; Lee, Yu Gyeong ; Hong, Ju Bong ; Lee, In Gyu ;
ALGAE, volume 12, issue 1, 1997, Pages 53~53
A simple d for RNA extraction from marine macroalgae was warked out. RNA was extracted in the presence of phenol to inhibit RNase activity and collected by LiCl precipitation to remove polysaccharide. This method was applied to Porphyra yezoensis, Griffithsia japonica, Dilophus okamurae, Scytosiphon lomentaria, Enteromorpha linza, Cladaphora sp., and Chaetomorpha spiralis. Electrophoresis of RNA from these algae showed distinct bands of RNA upon nucleic acid-specific staining. mRNA was isolated from the total RNA extracted from Griffithsia japonica, and the total RNA and mRNA were electrophoresed and hybridized wig the actin gene of Dictyosteliurn discoideum as a probe. A distinct band at 1.5 kb was detected from the RNA blot hybridization. The quality of mRNA was also good enough for the synthesis of cDNA on the purpose of cDNA library construction.