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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
The Korean Society of Phycology
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Volume & Issues
Volume 15, Issue 6 - 00 2000
Volume 15, Issue 4 - 00 2000
Volume 15, Issue 3 - 00 2000
Volume 15, Issue 2 - 00 2000
Volume 15, Issue 1 - 00 2000
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Purification and Characterization of Rubisco in Porphyra okamurae Ueda
최재석 ; 정익교 ; 전방옥 ;
ALGAE, volume 15, issue 3, 2000, Pages 155~155
Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) was extracted and purified from the fresh thalli of Porphyra okamurae Ueda, and the enzymatic properties were investigated. The isolation procedure for purifying rubisco involved precipitation with 18% (w/v) PEG and 200 mM Mg^(2+) then ion exchange chromatography on Toyopearl DEAE-650 and gel chromatography on Sephadex G-200. The purified rubisco was partially contaminated by phycobiliprotein. The final purification factor was 6.80 and the specific activity was 125.13 nmole CO₂/㎎ protein/min. The native molecular weight of the purified rubisco was estimated to be 540 kD by Sephacryl S-300 gel filtration chromatography. The enzyme was thought to have a conventional L_8S_8 composition. The molecular weight of large subunit and small subunit were 54.0 kD and 16.7 kD, respectively. The enzyme showed a maximum activity at 20 mM Mg^(2+), 20 mM NaHCO₃ and 2.5 mM RuBP. The apparent Km values for NaHCO₃ and RuBP were 3.65 mM and 1.2 mM respectively. These data provided some fundamental knowledge to seaweed rubisco that had not been studied thoroughly, and were compared with those from terrestrial plants and other algae to stress the unique features of rhodophytic rubisco.
Influences of Salinity on the Nitrate and Phosphate Removal by Fucus serratus, F. spiralis and F. vesiculosus (Phaeophyta)
ALGAE, volume 15, issue 3, 2000, Pages 163~163
The influences of salinities (8.5, 17, 25.5 and 34‰) on the removal of nitrate and phosphate by Fucus serratus, F. spiralis and F. vesiculosus were investigated. The total amounts of nitrate and phosphate taken up by F. serratus were 93.8% and 81.1% during 96 hours, respectively. And the removed amounts were 57.3% of N, 88.0% of P in F. spiralis, 61.5% of N and 87.2% of P in F. vesiculosus. Nitrate uptake by F. spiralis was promoted less than 34‰ of salinity. The uptake rates of nitrate and phosphate by salinity treatment were high at the first one hour during the culture period of 96 hours. The accumulated uptakes of nitrate and phosphate showed that the three Fucus species could remove 13.5-19.9 μ㏖ N·g^(-1) and 1.4-1.9 μ㏖ P·g^(-1) even under the medium of 8.5‰ salinity. Therefore, the nitrate and phosphate removal of the three fucoids did not affected by low salinity.
Distribution of Spirogyra spp. (Chlorophyta) in Response to Stream pH
김영환 ; 최상일 ;
ALGAE, volume 15, issue 3, 2000, Pages 169~169
Distribution and abundance of Spirogyra spp., the commonest macroscopic freshwater algae in Korea, were examined by the field survey. The pH value of waters in which plants of Spirogyra spp. grow was determined during the period of June-December 1999 in the central region of Korea. Of nine water systems where Spirogyra spp. grow, the minimum pH value was 5.6 and the maximum was 9.2. A total of 11 species of Spirogyra (S. arta, S. crassa, S. ellipsospora, S. jugaIis, S. majuscula, S. mirabilis, S. nitida, S. porangabae, S. setiformis, S. ternata, S. turfosa) was observed. Among these, S. ternata occurred at six stations, and S. arta, S. crassa and S. setiformis occurred at two sites. Biomass value of S. spp. varied between sites and seasons. S. ellipsospora, S. jugalis, S. majuscula and S. turfosa appeared to be abundant in slightly acidic to neutral conditions. On the other hand, S. crassa, S, mirabilis, S. nitida and S. porangabae were rather abundant in neutral to slightly basic conditions.
Discrimination of Three Species of Dinoflagellates Cochlodinium polykrikoides, Gyrodinium impudicum and Gymnodinium catenatum using FITC- lectins
조은섭 ; 김기영 ; 오봉철 ; Lesley L. Rhodes ; 이재동 ;
ALGAE, volume 15, issue 3, 2000, Pages 175~175
We applied fluorescent lectins to discriminate between toxic and non-toxic dinoflagellates in order to develop and improve the monitoring and prediction programs in Korean coastal waters. The differentiation between C. polykrikoides and G. impudicum using fluorescent-labeled lectins was achieved by the application of lectins, ECA, HPA and WGA. The lectin WGA was found to be useful tool for discrimination of toxic G. catenatum from non-toxic G. impudicum.
Construction of Artificial Seaweed Beds;Using the Spore Bag Method
최창근 ; Yukihiko Serisawa ; Masao Ohno ; 손철현 ;
ALGAE, volume 15, issue 3, 2000, Pages 179~179
Kelp forests and Sargassum beds not only are commercially important in themselves but also play an important role in spawning, breeding and feeding grounds for many kind of fish, shellfish, lobster and other important fisheries resources (Komatsu et al. 1982; Komatsu 1985; Ohno et al. 1990; Watanuki and Yamamoto 1990; Terawaki et al. 1998). The distributions of kelp forests comprising of the Ecklonia and Eisenia species are restricted to warm-water regions. A number of ecological studies on these plants have been undertaken with particular attention focused the re-establishment of kelp forests. This is due to declining populations that are a result of various factors, including human activities (Tsutsui et al. 1996). Thus, there has been development of construction techniques of artificial foundation, for kelp forests, and transplanting techniques of seed and adult marine algae, on artificial foundations (Hasegawa et al. 1995).Techniques of formation for seaweed bed may fall under either of the following classifications: 1. Spore dispersal technique; 2. The spore bag technique; 3. Ropeseeding technique; 4. Adult-plant transplantation method; 5. Concrete blocks; 6. The threading technique; 7. Gravel-bag technique; and 8. Transplantation of young plants (Largo and Ohno 1993).In this study, we used the spore bag on the roof of an artificial iron reef and a natural rock for a seaweed bed. The present paper describes the formation of seaweed beds by the spore bag method on an artificial iron reef and a natural rock (habitat).