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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
The Korean Society of Phycology
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Volume 15, Issue 6 - 00 2000
Volume 15, Issue 4 - 00 2000
Volume 15, Issue 3 - 00 2000
Volume 15, Issue 2 - 00 2000
Volume 15, Issue 1 - 00 2000
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ITS 2 Sequences of Gelidium amansii Populations from Korea
김종인 ; 이재완 ; 이해복 ;
ALGAE, volume 15, issue 6, 2000, Pages 125~125
In order to elucidate the identity of formas in Gelidium amansii, nuclear ribosomal DNA ITS 2 region was analysed with eight populations belonging to three formas collected in Korea. For data analysis previously published sequences of taxa were also used. The ITS 2 sequence data were used in parsimony, maximum likelihood, and distance analyses. Our analysis of G. amansii forms from Korea showed the exactly same sequences of 371-bp long ITS 2, but one base substitution. This result indicates that the intraspecific separation of G. amansii species cannot be supported by the molecular data analysed.
Simple and Rapid Isolation of Plasmids from Porphyra tenera
최학선 ; 최경희 ; 류태형 ;
ALGAE, volume 15, issue 6, 2000, Pages 133~133
Searching algal plasmids was the target of current efforts in developing transformation system in algae. To exploit the construction of a vector system, isolation of pure plasmid DNA was indispensable demand. However, currently available plasmid purification methods from algae were time-consuming and inflicted unavoidable impurity problems on the DNA preparation. Therefore, a plasmid isolation method originally described for E. coli by Birnboim and Doly (Sambrock et al. 1989) was adopted to Porphyra to isolate plasmid from P. tenera thalli. The modified method was constituted a total DNA isolation procedure and the SDS-alkaline lysis procedure without using ultracentrifuge. The plasmid DNAs isolated by the modified method were pure enough for the following molecular biological works.
The Cytoskeletal Network during Division in the Marine Dinoflagellates, Pyrocystis lunula and P. noctiluca
서경석 ; Lawrence Fritz ;
ALGAE, volume 15, issue 6, 2000, Pages 137~137
When forming daughter cells in the two oceanic non-motile dinoflagellates P. lunula (Schutt) Schu¨tt and P. noctiluca Murray, the complex cytoskeleton network of microtubules in the mother cyst cell reforms around each new daughter cell. Our results suggest that the cortical cytoskeleton network of Pyrocystis does not disappear, as known for other dinoflagellates, but rather passes from the surrounding mother cell wall area to the daughter cell wall area.
Mitochondrial Dynamics in Red Algae. 1. Monospore Germination in Audouinella botryocarpa (Acrochaetiales)
David J. Garbary ; Pei Zuchang ;
ALGAE, volume 15, issue 6, 2000, Pages 143~143
Mitochondrial distribution was examined during monospore germination in Audouinella botryocarpa (Harvey) Woelkerling using fluorescence microscopy and staining with DiOC_6(3) and DASPEI. Mitochondria varied in shape from spherical to ovoid or irregular. In released monospores mitochondria were evenly distributed. During germination many mitochondria became concentrated where the single germination tube emerged, and large mitochondrial complexes developed. This dense aggregation of mitochondria became associated with tip growth of the germ tube, and was retained through at least several apical cell divisions of the sporeling. In addition, many mitochondria remained in the spore and formed large complexes associated with the transverse wall of the developing filament. They were retained during the apical cell division of the sporeling and subsequently declined in size and number.
Mitochondrial Dynamics in Red Algae. 2. Monosporogenesis in Audouinella botryocarpa (Acrochaetiales)
David J. Garbary ; Pei Zuchang ;
ALGAE, volume 15, issue 6, 2000, Pages 149~149
The shape, number and distribution of mitochondria were examined during monosporogenesis in Audouinella botryocarpa using fluorescence microscopy and staining with DiOC_6(3) and DASPEI. Mitochondria are variable in shape and range from spherical to ovoid or irregular. There is an increase mitochondria) number during sporogenesis from about 75 to 200, and mitochondria) number is closely correlated with sporangial size (r = 0.90). Mitochondria are evenly distributed in monosporangial initials. At least ten, large mitochondria) complexes occur early in sporangial development, and these are evenly scattered throughout the cell. Subsequently, these complexes decrease and become restricted to the central portion of monosporangia. Just prior to spore release about one half of the mitochondria remain aggregated in this region. Patterns of mitochondria) distribution and abundance likely reflect changes in metabolic demands and in nuclear/cytoplasmic interactions during sporogenesis.