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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 10, Issue 4 - Dec 1997
Volume 10, Issue 3 - Sep 1997
Volume 10, Issue 2 - Jun 1997
Volume 10, Issue 1 - Mar 1997
Selecting the target year
Efficient Transformation of Trifolium repens L. Using Acetosyringone
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 107~113
Transformants of White Clover(Trifolium repens L.) were efficiently produced from immature seed derived callus cocultivated with Agrobacterium twnefaciens LBA4404 harboring plant binary vector. pBI121, using acetosyringone. The mean frequencies of transformants on the two kanamycin-containing media were 16 to 19% when the immature seed-derived calli were infected with bacteria cultured in the presence of 100
M acetosyringone compared with 7% in media without acetosyringone. Transgenic white clover was subject to molecular analysis for integration into plant nuclear genome and expression of
-glucuronidase(GUS) gene. PCR and Northern blot analyses demonstrated that GUS gene was integrated into white clover nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by spectrophotometric assay of GUS activity.
Effect of Plant Growth Regulators and Medium Salt Strength on In Vitro Propagation of Belamcanda chinensis DC
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 114~121
The effects of plant growth regulators on callus formation and organogenesis in shoot tip explant of Belancanda chinensis were examined. Shoot tip explants cultured in full salt strength of MT(Murashige and Tucker) medium containing 2,4-D 1.0 or 2.0mg/l were vigorously formed callus. Full salt strength of MT medium and 1/2 MT medium supplemented with zeatin 1.0mg/l were more effective than that with combination treatments of 2,4-D on the formation of shoots from calli. When shoots regenerated from shoot tips were transplanted into 1/2 MT medium added with 1.0mg/l, 41% of shoots formed roots.
Effect of Embryo Maturity and Medium on Callus Formation and Plant Regeneration from Immature Embryo of Eleutherococcus senticosus
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 122~127
This study was conducted to establish mass propagation system from the tissue culture using immature embroys in Eleutherococus senticosus. Immature embroys from seeds were removed under the microscope and placed on modified SH and WPM medium containing several plant growth regulators. The calli were well formed on media containing 1mg/l of 2,4-D on modified SH medium and 1mg/l of 2,4-D and 3mg/l of TDZ on WPM medium. Shoot regeneration was better on modified SH or WPM medium with combination of high concentration of TDZ and low concentration of 2,4-D. Treatment of 2,4-D alone was better than treatment of TDZ alone in callus induction on modified SH medium but plant regeneration reversed. Treatment of 2.4-D and TDZ combination was better than treatment of 2,4-D alone in callus induction on WPM medium. The results of callus formation and shoot regeneration on WPM media differed to those of SH media. The rate of callus formation was nearly 83% when 2,4-D was added to SH medium on concentration of 1mg/l. The rate of callus formation was nearly 38% when combination treatment of 2,4-D 1mg/l and TDZ 3mg/l was added to WPM medium. Also, plant regeneration differed depending on the mature degree of immature embryo.
Resistance of Varieties Bred by Crossing with Asominori to Bacterial Leaf Blight
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 128~134
This study was conducted to test on the resistance of varieties bred by crossing with Asominori to bacterial leaf blight. Nakdongbyeo and Dongjinbyeo which were susceptible to HB 9011, 8 resistant varieties including Ilmibyeo derived from Asominori and Asominori, Hwangok, 13 varieties including Chukoku 45 which were resistance to HB 9011, HB 9022 and HB 9033 were used to screen their res ponce depending on the various screening methods such as the true resistance, the secondary infection resistance and the disease common field test methods, and the results are as follows: Among 13 varieties tested, 11 varieties including llmibyeo showed tme resistance to HB 9011. Less than 1.0cm of disease lesion were developed on these varieties. Disease lesion was not developed on most of the Asominori lines including Daechongbyeo against IIB9011 and 1lmibyeo was also resistance to HB 9011, on this variety disease lesion area rate was 1.2%, and 7 varieties including Hwajinbyeo showed field resistance to HB 9022. Disease lesion area rate were 19.6% on Nakdongbyeo. 15.6% on Dongjinbyeo, from 3.0% to 2.4% on Asominori lines, and 0.5% on the Asominori when screened at disease common area. Disease was not developed on Keumnambyeo. Significant correlation coefficiences were found between the results from the test methods of the true resistance, the field resistance ancl the field resistance at disease common field tests on Asominori lines, but in some cases, even the varieties on which disease lesions developed, showed field resistance to HB 9022 and HB 9033.
Agronomic Characteristics of "Sikbangpoong 1" a New High Variety of Peucedanum japonicum Thunberg
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 135~139
A new Sikbangpoong(Peucedanum japonicum Thunberg) variety, 'Sikbangpoog 1', was developed through a pure line selection at the Kyougpuk Provincial RDA during the period of 1990 to 1995. The variety was characterized to have higher stem height, leaf number and seed production, and larger root diameter compared with the check variety of Bonghwa Sikbangpoong but plant height of 'Siknagpoong 1' was similar to that of the check variety and it was also more tolerant to water and heat stress. Root color of 'Sikbangpoong 1' was yellowish brown and root shape of the variety was straight and conical. The root yields of Sikbangpoong 1 in yield trial, regional adaptation trial and farmer's field trial were always 10-28% higher than those of the check variety.
Effect of Planting Method on the Growth and Yield of Rehmannia glutinosa Liboschitz
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 140~144
This experiment was carried out to investigate the proper planting method of Rehmannia glutinasa Liboschitz. A Chungbuk local cultivar was sown on the seed bed of 200cm with the planting density of 30cm(6 rows)
8.5cm(30 rhizome per
). The experimental design was randomized block design with 3 replications. And the result was summarized as follows. The emergence date of level Planting(control : 0
) was May 23, but the emergence dates of Oblique planting(45
) and Upright planting(90
) treatments were later than the control by 2~4 days. And the leaf length and the ratio of leaf length/leaf width were not significantly different. As compared with the 29.2cm and 10.0mm of the rhizome length and the rhizome diameter of level planting treatment, the rhizome length of Oblique planting treatment was decreased by 3.4cm and 0 mm. The length and diameter of rhizome of the Upright planting treatment was decreased by 2.8cm and 1.0mm. And the order of sugar content was level Planting > Oblique planting > Upright planting. The total yields were decreased by 9% in the Oblique planting and by 15% in the Upright planting compared to 1,203kg/10a in the control, and the commercial yields showed the same tendency. The level planting treatment was found to be most proper for sowing of Rehmannia glutinasa Liboschitz in the middle inland region of Korea.
Effect of Fertilizers on Yield and Storage Quality of Early Maturing Variety in Onion
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 145~150
To find out the optimum fertilizer level for onion, early maturing cultivar Fechongjoseng, experiment with four fertilizer levels was conducted of the field of the paddy and upland in Muan and Changyeong from Sep.1993 to Feb.1995. The yield character of onion was higher and rate of the rottenness was lowest under the treatment of
=28-9.5-23Kg/10a in the soil of paddy and upland field of Muan area. But the onion yield was excellent and rate of the rottenness was lowest under the treatments,
=21-19-17.3Kg/10a, in the soil of paddy field and
=28-9.5-23Kg/10a, in the soil of upland field of Changyeong area.
Climatic Influence on Seed Oil Concentration in Soybean (Glycine max)
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 151~158
This study was carried out to identify how soybean seed oil is influenced by climatic factors and to investigate how genotypes differ in their responses. Twelve lines selected were studied in 13 environments of North Carolina. Responses of oil concentration and total seed oil to climatic variables were investigated using a linear regression model. The best response models were determined. There were wide climatic effects in oil concentration and total seed oil. The lowest oil concentration environment was characterized by the most HTD and the smallest VADTRg and the lowest total oil environment was distinguished by the largest VADTRa and the smallest VMnDT. For oil concentration, most lines except for NC107 responded negatively to MxDT, HTD, ADT, and ADTRg, although they had different degrees of sensitivities, indication that warmer temperature may result in decreased oil concentration. All lines responded positively to VMnDT, VADTRg, and ADRa, although they had different degrees of sensitivities, suggesting that larger variation in minimum daily temperature and average daily temperature range and more average daily rain may result in increased oil concentration. Eleven lines had best response models with 1 to 3 variables. However, although NC109 did not show a significant sensitivity to any variable, it had the best response model with 2 significant variables, demonstrating that an interaction between 2 variables might be more critical in determining oil concentration than one variable.
Effect of Planting Data and Density on Growth and Yield of Cnidium officinale Makino
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 159~164
Field experiment for two years was conducted at Ulleung island to determine the optimum planting date and density of Cnidium officinale Makino. Early planting(Mar. 20) showed the highest yield by 2,880kg per ha as dry wt. Planting time after Mar. 20 was decreased. Late planting had higher occurrence of diseases and insect damage. Early planting had higher extract contents than late planting without violation of the Korean crude drug regulation such as ash contents. Although plants grew better in lower density, they had fewer growing points per plant which actually determine the yield components. Planting density did not affected extract contents in Cnidium officinale Makino. High density planting(
)yielded 33% than those of lower planting density(
Effect of Root Preservation of Epimedium koreanum on Bud Germination and Early Growth
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 165~168
To establish the culture practices for mass propagation of Epimedium koreanum, root propagules were preserved with different methods. Bud emergence rate and number of multiple buds were the highest in root segments obtained from roots with buds which were preserved in open-burial. Plant height, leaf area, and number of new buds were the highest in root segments obtained from roots preserved with stratification.
Weed Control in Herb Field by Means of Physical Treatments I.Weed Occurrence in Herb Field and Effect of
and Heating on the Germination of Weed Seeds.
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 169~174
This study was conducted to obtain basic information needed to develop the effective weed control method by using thermal weeder which uses LPG as fuel. For survey of weed distribution in herb field, weed species were investigated at two kinds of herb field of Codonopsis lanveolata and Aster scaber located in Hoengseongkun and Hwacheonkun, Kangwondo. In Codonopsis lanveolata field, Digitaria sanguinalis and Persicaria viridis L were dominated. In Aster scaber field, Digitaria sanguinalis and Erigeron canadensis were dominated. In general, Grasses weeds in herb were more dominated than other broadleaf and Cyperaceae weeds. The treatments of
in weed seeds were more effective for germination of several weeds, compared with that nontreated weed seeds. However, the rate of germination differed depending on weed species and tereatment time of
. Also, viability of weed seeds was significantly affected by irradiation time and temperature. As the temterature and treatment time of irradiation increased, the rate of germination was decreased.
Effect of Organic Fertilizers Application in Root Yield and Saikosaponin Contents in Bupleurum falcatum L.
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 175~182
This study was carried out to investigate the effect of several organic fertilizers on the growth characteristics, root yield and the levels of the efficacious constitutents saikosaponins in Bupleurum falcatum L. in the field of Chonnam RDA. Naju, 1994. Five organic fertilizers were used ; rice straw manure(RSM), rice straw(byproduct of mushroom)manure[RS(BM)M], pig-dropping sawdust manure(PSM),poultry manure(PM)and sawdust(byproduct of mushroom) manure[S(BM)M]. The results were summarized as follows ; The chemical properties, such as organic matter, available phosphate,
, CaO, and MgO of soil were increased on all plots with the addition of organic fertilizers, specifically in PM+RS(BM)M application. PM+RS(BM)M treatment was higher in emergence rate and better in the growth charaters of shoot, root parts of plants than those of conventional fertilization and the highest dried root yield resulted from using 39% PM+RS(BM)M treatment. Thus, the organic constituents of the plant leaves were affected in the same ways by all treatments, but the T-N,
, CaO and MgO contents of the roots were highly increased in PM+RS(BM)M treatment, also the total saikosaponin levels in the roots increased with different organic fertilizers in the following order : 1.70% in PM+RS(BM) treatment, 1.66% in RS(BM) treatment, and 1.57% in RSM+N-P-K treatment. There were significant positive correlation observed between the growth characters of shoot, root parts of plants, yield and contents of T-N,
, total-saikosaponin in B. falcatum roots.
Growth Characteristics and Content of Sarsasapogenin in Different Plant Parts of Anemarrhena asphodeloides Bunge
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 183~187
This study was carried out to find the growth characteristics and content of sarsasapogenin in different plant parts of Anemarrhena asphodeloides Bunge. Five native-cultivars were collected and evaluated for several agronomic traits. The collected native-cultivars were classified into two seed-attached peduncle and vegetative propagation types. Seed-attached peduncle lines were predominance of growth traits than vegetative propagation. For the content of sarsasapogenin in each part was investigated, BuOH extarct of 'timo' was developed on silica gel 60G plate using elution solvent(
: Methyl ethyl Ketone : EtOH = 11 : 2 : 0.5). The developed plate were examined using Dual Wavelength Zig - Zag using. Content of sarsasapogenin in main root and lateral root were respectively 1.67mg/g and 1.31mg/g.
The Study of Superoxide dismutase (SOD) and SOD-mimic Compounds in Panax ginseng C.A.Meyer
/ U ;
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 188~193
Panax ginseng C.A.Meyer,1 to 5 years old were electrophored and were stained for SOD activity. The result indicated a total of 13 distinct form of the enzyme and the pattern of achromatic bands were not different according to ages. Nine of the enzyme activities were eliminated with cyanide or peroxide treatment and were resistant to treatment of chloroform plus ethanol. It suggested that they may be cupro-zinc containing SOD, whereas four were cyanide or peroxide resistant and were eliminated with cholroform plus ethanol treatment. They may be manganese containing SOD. Ginseng roos. 1 to 5 years old were analyzed for their SOD measurement of SOD activities of all extracts, the significant difference of SOD activities were not shown according to ages. All ginseng extracts had the total SOD activities of all extracts, the significant difference of SOD activities were not shown according to ages. All ginseng extracts had the total SOD activities of about 700-800 unit/g of fresh weight. Therefore, the SOD activities from SOD-mimic compounds were higher than one from SOD. The ratio between the SOD activity from SOD-mimic compounds and one from true SOD was approximately 2:1 to 3:1.
Notes on the Korean Higher Fungi (XIII)
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 194~199
Many higher fungi were collected at Mt. Moak Provincial Park, Mt. Yonsuk in Wangju-Kun from May to October 1995. They were identified and according to the results, one genus and eight species were newly to Korea. Genus Stereopsis was newly to Korea. Hygrophorus arbustivus, Tricholoma imbricatwn, Mycenafragillima, Cortinarius spilomeus, C. saturninus, Dermocybe semisanguinea, Stereopsis burtianum and Jansia bomeensis were newly to Korea.
Utilization of Molecular Markers in Plant Genetics and Breeding
Korean Journal of Plant Resources, volume 10, issue 2, 1997, Pages 200~210
The understanding on the plant genome is accelerated with the fast advance of molecular biological techniques. The molecular dissecting of the plant genome has made possible the precise genotyping the plants, which can be utilized for molecular breeding program. As well, the molecular cloning of genes interested can facilitate the process of gene transfer between intra-and inter-generic taxa. Moreover, the manipulation of the agronomically important QTL genes, which can be rarely performed by the conventional genetic methods, is also possible by the utilization of molecular markers. In addition to these genetical applications, molecular markers are useful in the areas of plant taxonomy and management of germplasm by fingerprinting analysis. This paper describes the theoretical aspects marker technologies and practical applications of each marker technique.