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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 10, Issue 4 - Dec 1997
Volume 10, Issue 3 - Sep 1997
Volume 10, Issue 2 - Jun 1997
Volume 10, Issue 1 - Mar 1997
Selecting the target year
Screening for Cytotoxicity of Crude Extracts from Fruit on Leukaemia Cells in Citrus and Related Genera
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 213~220
The present study has been undertaken to characterize availability of citrus as a medicinal plant with antineoplastic property. The crude extracts from 40 species of fruits with 12 species of the local Citrus in Cheju island were evaluated on their potential activities against mouse P388 lymphocytic leukaemia in vitro. The percent cytotoxicity varied from 25.40 to 97.94% at a concentration of
. Among 40 spp., 8 species showed high toxicity more than 90% against P388 cells and Cheongkyool(C. nippokoreana) exhibited the most cytotoxicity as 97.94%(
). Nine varieties of C. junos were showed insiginicant cytotoxicity. In trifoliate orange, immature fruit was stronger than mature and peel extract showed higher cytotoxicity(
) than the other tissues. Hexane fraction from methanol(MeOH) extract of trifoliate orange showed highly significant inhibition of cell growth(
). In addition, its cytotoxicity increased remarkably from 3.95 to
as exposure time legthened. Cytotoxic activities of crude extracts were decreased considerably during a six months storage period. It was apparent that there is considerable variation in cytotoxicity, depending upon species, maturity and storage time of extracts. There was no meaningful cytotoxic difference between archicitrus and metacitrus in the genus Citrus.
Effects of Sulfur Nutritional Forms on Accumulation of Seed Storage Proteins in Soybean (Glycine max)
/ N ;
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 221~226
Improvement of seed protein quality might be an essential issus in soybean and would give more profit directly to both farmers and users. This study was carried out to investigate the effects of reduced-S form(s) on seed storage protein components in soybean during seed filling stages. The reduced-S forms during seed fill were sodium thiosulfate, sodium sulfite, sodium sulfide, thioaceteat,
-mercaptoethanol, thiourea, thiamine-HCI, L-cysteine, L-cystine, and L-methionine. Seed storage protein concentration did not appear to be affected by any reduced-S forms. However, glycinin and
-conglycinin concentration seemed to be changed greatly by L-methionine. This resulted in the increase in the 11S/7S ratio(3.58). Among the
-subunit was not accumulated at all.
-subunit concentration appeared to be decreased and
-subunit concentration was not altered in comparison with sulfate control. Also,
-conglycine concentration, especially
-subunit concentration, tended to be decreased with L-cystine treatment, resulting in an increase in the 11S/7S ratio(1.83). The glycinin concentration tended to be increased at the expense of the decrease in the
-conglycinin concentration. Therefore, it is suggested that enhancing soybean protein quality would be achieved by improving metabolic pathways of S assimilation in soybean plants during seed filling period under sulfate-sufficient condition.
Antioxidative Activity of Urushiol Derivatives from the Sap of Lacquer Tree (Rhus vernicifera Stokes)
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 227~230
The authors isolated four olefinic catechols, commonly referred to as urushiol, from the sap of Korean lacquer tree(Rhus vernicifera STOKES) with stronger antioxidative activities than
. The hexane extract with a free radical scavenging activity was purified by silica and ODS gel column chromatography. The active compounds were identified by MS and
as 3-[8'(Z),11'(Z),14'-pentadecatrienyl]catechol, 3-[8'(Z),11'(Z)-pentadecadienyl]catechol, 3-[8'(Z)-pentadecenyl] catechol, and 3-pentadecylcatechol. All of these compounds showed strong free radical scavenging activities on 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical, in which 3-pentadecylacatechol exhibited the highest activity (
). They also showed a significant inhibitory activity on lipid peroxidation (
: 2.1 - 3.5
). The antioxidative activity of 3-pentadecylcatechol on DPPH radical and lipid peroxidation is approximately two times greater than that of
-tocopherol. The results suggest that the urushiol derivatices may contribute to the preservative characteristics effective against oxidative stress and could be a good source for industrial applications including a coating material.
Antifungal Activity of Urushiol Components in the Sap of Korean Lacquer Tree (Rhus vernicifera Stokes)
Kim, Myong-Jo ; Kim, Chang-Jin ; Kwak, Sang-Soo ;
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 231~234
Four urushiol components isolated from the sap of Korean lacquer tree(Rhus vernicifera Stokes) showed a strong antifungal activity, but they have no or low activity the bacteria and yeasts. Among them, 3-pentadecylcatechol marked the highest activity on the spore germination of Cladosporium herbarum (
Effect of Metals on Anti-Oxidase Activity and Isozyme patterns in Brassica juncea
Jeong, Hyung-Jin ; Lee, In-Jung ; Sung, Mi-Hyang ;
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 235~240
To study the effects of metal ions on the activity of anti-oxidase enzymes, the activity of superoxide dismutase (SOD) and peroxidase (POD) and isozyme patterns of Brassica juncea have been studied after treating with CD, Cu, Zn, and Al. The activity of SOD after treating with metal ions was higher than that of untreated control. SOD activity in leaves increased by treatment of 50 ppm of Zn and 500 ppm of Al. POD in stems gave highest activity after treating with 500 ppm of Cu. When the activity was compared by plant parts, lowest POD activity was observed in leaves in which protein content was higher than other tissues. When the activity was expressed as percentage of control, SOD activity was increased after treating with metal ions. SOD activity in leaves and roots of metal treated plant was significantly increased under the metal ions stress conditions. In the roots of 50 ppm of Zn treated plant, SOD activity was extremly high. POD activity was inhibited with Cd and Zn treatment in all parts of the plant. However, in leaves and stems, there was marked increase in activity after treating with Cu. The patterns of SOD isozyme after metal treatment show that two bands were stained in all metal ion treated and that no new band appeared. POD isozyme band intensity resulting from the treatment of metal ions was in order of roots > stems > leaves, but there was no significant difference.
Construction of Linkage Map Using RAPD and SSR Markers in Soybean (Glycine max)
/ J ;
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 241~246
Linkage maps based on molecular markers are valuable tools in plant breeding and genetic studies. A population of 76 RI lines from the mating of A3733 and PI437.088 was evaluated with Random Amplified Polymorphic DNA(RAPD) and Simple Sequence Repeats (SSR) markers to create soybean molecular linkage map, 302 RAPD and 21 SSR markers were genetically linked and formed forty linkage groups. These linkage groups spanned a genetic distance of 1,775 cM. The average distance between markers was 5.5 cM.
RAPD Loci for Seed Protein and Oil Content in Soybean (Glycine max)
/ J ;
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 247~249
Seed protein and oil content is important trait in the soybean. Both seed protein and oil content in this plant species is inherited quantitatively. A 68-plant
segregation population derived from a mating between Mercury and PI 467.468 was evaluated with random amplified polymorphic DNA (RAPD) markers to identify QTL related to seed protein and oil content. Marker OPB12 was found to be associated with differences in seed protein content. Four markers, OPA09b, OPM07b, OPC14, and OPN11b had highly significant effects on seed oil content. By interval mapping, the interval between marker OPK3c and OPQ1b on linkage group 13 contained a QTL that explained 25.7% variation for seed oil content.
Analysis of Phylogenetic Relationships among Medicago Species by Proteins Banding Patterns and RFLP Markers
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 250~257
The relationship of nine Medicago species belonging to four subgenera were analyzed by using SDS-PAGE and restriction fragment length polymorphism (RELP) methodologies. Sixty-eight bands of alcohol and salt soluble proteins and 85-133 RFLP markers were used to estimate the genetic distance among the species. These species were clustered together at around 0.1 to 0.4 level of distance for both kind of markers, indicating that Medicago species have a large genetic similarity. A combined cluster diagram, at a dissimilarity level of 0.3, differentiated nine species in four groups: group 1, M. littoralis , M. truncatulam, M.scutellata and M. rigidula; group 2, M. sativa ; group 3, M. lupulina ; group 4, M. orbicularis, M. radiata and M. minima. All of them, but except for M. minima. corrensponded to the existing four subgenera of the genus Medicago classified by Lesins and Lesins(1979).The most similar species were M. littoralis and M. trucatula and the most dissimilar one was M. lupulina. In separate cluster diagrams based on RFLP and protein markers, some differences were observed. In the case of RFLP or DNA markers, M. sativa (alfalfa) was distantly clustered with other Medicago species. But in the case of protein markers, M. sativa was closely clustered with M. scutellata, M. littorulis and M. truncatula.
Evaluation of Genetic Diversity among Korean Wild Codonopsis lanceolata by Using RAPD
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 258~264
The introduction of molecular biology methodologies to plant improvement programs offers an invaluable opportunity for extensive germplasm characterization. We have applied the developed technique of random amplification of polymorphic DNA(RAPD)to the analysis of evaluating genetic diversity among Korean wild Codonopsis lanceolata. A total of 340 polymorpic hands were gernerated on agarose- and polyacrylamide-gel by 19 primers of abitrary sequence. grouped by cluster analysis using sample matching coefficients of similarity. Among of the samples. the minimum genetic distance value was obtained between sample no. 1(Girisan) and no. 2(Girisan), and the largest value between sample no. 11(Sulaksan) and no. 17(Sulaksan).In separate cluster dendrograms based on agareose - and polyacryamide-gel. some differences were observed; In the case of agarose gel,41 samples could be devided into 7 groups at below about 0.44 level of distance. However they were divided into 6 gourps at below about 0.40 level of distance in the case of polyacrylamide gel. These results showed that polymophic data in agrose were not grouped to wild plant selected from each mountainous district except for wild plants selected from Sulaksan and Chiaksan. We believe that polyacrylamide-RAPD is a superior method for detecting DNA polymorphism compared to agarose-RAPD method.
Notes on the Korean Ascomycetes (III)
Cho, Duck-Hyun ;
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 265~270
Many higher fungi of ascomycetes were collected at Mt. Jiri National Park from May to October 1996. They were identified and according to the results, Orbilia, Pezicula, Creopus, and Lasiospheria were new genera to Korea. Dasyscyphus bicolor, Orbilia coccinella, Pezicula acercicla, Mollisia revincta, Hypocera citina, Creopus gelatinosus, Lasiospheria ovina, and Rosellinia thelena were newly to Korea.
Native Environment and Growth Characteristics of Dendropanax morbifera Lev. In Korea
Park, Seong-Kyu ; Park, Kyong-Ju ; Lee, Jong il ;
Korean Journal of Plant Resources, volume 10, issue 3, 1997, Pages 271~273
This study was carried out to obtain basic envirinmental information of the native area and growth characteristics at different age of Dendropanax morbifera LEV. In the southern Korea. The Dendropanax morbifera LEV. Was grown naturally at Wando, Cheju(Mt. Halla), Yecheon(Kermoon island),Sinan(Hong isand), Jindo(Mt. Chermchill) and Haenam(Mt. Taeroon), all between 30 to 450m above the sea level. The soil characterisitics of the native area had a pH of 5.3 to 5.4 with 8.4 to 9.8% content of organic matter and 18.2 to 21.3% soil moisture. Flowering starting in the middle of July begun when the trees were 6 years old. Six year old trees are 129 cm in stem height, 34 mm in stem diameter,15.4 in number of leaves with petioles 12.9 cm long. Xylem sap can be obtained from over 10 year old trees that grew well and have over 10 cm stems in diameter.