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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 16, Issue 3 - Oct 2003
Volume 16, Issue 2 - Jun 2003
Volume 16, Issue 1 - Feb 2003
Selecting the target year
Fruitbody Formation of Cordyceps militaris in Allomyrina dichotoma Linnaeus
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 1~7
Three strains(CHO-7208, CHO-7845, CHO-7846) of the Cordyceps were used for mass production by artifical cultivation with Allomyrina dichotoma larva. The mycelium length of Cordyceps mititaris on PDA was grown to 25
2mm(CHO-7208) and 26
21mm(CHO-7845) and 16
2mm (CHO-7846) for 13days cultivation. The larva of Allomyrina dichotoma reared with starch, wheat flour and rice. The best rear material were starch. The formation of fruitbody on media were possible with CHO-7208 and CHO-7846. The fruithbody length of CHO-7208 on A.dichotoma media were 51
5mm for 27 days culture. And then fruitbody length of CHO-7846 on same media were 56
5mm for 27 days culture. The larva of A.dichotoma media was excellent fruitbody formation of C. mititaris.
Major Characteristics of Burdock(Arctium lappa L.) Native to Yeong-Narn Region
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 8~14
This study was aimed to obtain basic information on the characteristics of local cultivars of burdock(Arctium lappa L.) grown in Yeong-Nam region. The characteristics of seeds were shown a remarkable diversity among the local cultivars. Germination rate was the highest at 25
and Munkyong' as the highest germination rate(42.2%) at l0
respectively. In Youchon' and 'Chilgok', about 10％ was bolted but the other was rarely or none. The range of root length was 62∼77cm and lateral root number was maximum 5.5('Koryong') and minimum 0.3('Songju). 'Chongdo I' contents 14.2mg/100g of tannin in root and 'Kyongsan', 'Koryong', 'kyongiu',’ Kumi' and 'Haman' taste not bitter in leaves.
Studies on the Distribution and Vegetation of Ambrosia artemisiifolia var, elatior Community Group in Jeju Island
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 15~24
The study was performed to understand the actual vegetation by using the phytosociological method on the Ambrosia artemisiifolia var. elatior community group in Jeju Island. The flora was composed of 48 families, 156 genera, 200 species, 28 varieties, 2 forma or 230 taxa. The Ambrosia artemisiifolia var. elatior community group vegetation was classified into two communities and one association; Erigeron annuus - Conyza sumatrensis, Bromus tectorum - Bidens pilosa, Setaria viridis - Eleusine indica community and Lactuco indicae - Humuletum japonicae association. Ambrosia artemisiifolia var. elatior community group was wide from the coastal areas up to the elevation of 1500 meter on Young sil of Mt. Halla. The highest dominance of the Ambrosia artemisiifolia var. elatior is 345 meter which is located near Seoneheul-ri Jocheon-eup, on the other hand the lowest one is located 1020 meter near Young-sil of Mt. Halla.
Transitional Element Contents of the Ginsengs, Keumsan Area
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 25~33
Different ages of the ginsengs were collected from the three soil areas, such as granite, phyllite and shale of Keumsan, with their field soils. Of the soils, phyllite and shale areas are high in the Ni, Cr, Co, Sc, V, As, Cu and Zn contents while the granite areas are low in the W, Pb, Th, U, Sn and Be contents. In the correlation coefficient of the soils, positive and negative relationships of the elements are mainly high in the shale and low in the granite area. Comparing between 2- and 3- year ginsengs, 2-year ginsengs are mainly high in most elements, especially for the granite and phyllite areas. In the regional comparisons among the same ages, the granite areas are mainly low and high in the shale area for the most of the elements. Comparisons among the parts of ginsengs suggest high contents in the most of the elements at the upper parts. In the comparisons between the top soils and ginseng compositions, top soils are mainly high and show similar increase/decrease trends between them. Following the transitional element contents, the granite area is best for the ginseng cultivation while , among the three areas, the shale area is worst.
Micropropagation from Corm Apical Meristems Culture of Freesia refrecta Hybrida
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 34~39
Corm apical meristem cultures of thirteen glasshouse freesia cultivars were tested to investigate the possibility of micropropagation using MS basal medium supplemented with 2,4-D, NAA(0.1, 0.5, 1.0mg/L, respectively) and BA (0.5∼2.0mg/L). The majority of the tested cultivars could be induced callus and shoot buds in all culture condition. The combinations of NAA and BA appeared superior to that of 2,4-D and BA depending on cultivars for callus induction and shoot formation. Among the cultivars, 'Golden Yellow' showed the highest regeneration capacity on MS media with 0.5mg/L NAA and 1.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoot from calli were obtained through successive subculture on MS medium supplimented with 0.5mg/L BA. In that condition, more than 60 ％ shoot regeneration and average of 25.1 shoots per explant was achieved. Transformed shoots on half-strength MS medium without plant growth regulators rooted easily.
Development of RT-PCR Kit for Diagnosis of Pathogenic Agent of Ginseng Root Rot in the Ginseng Field
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 40~48
Cylindrocarpon destructans is the major pathogen inducing the root rot disease in ginseng. Up to now, there is no reliable and convenient method to analyze the spore density or population of this pathogen in ginseng-growing soil or any contaminated farmlands. Therefore, it will be very valuable to develop a new and reliable method in detecting the spore of this pathogen. In this study, a molecular biological technique using two step nested PCR method, was developed. Two universal ITS primers, ITS5F and ITS4R were used in the first round of PCR to amplify a fragment of ITS region from the genomic DNA of C. destructans. The specific prmers Nest 1 and Nest 2 were designed and used in the second round of PCR to amplify a inner fragment from the first round PCR product of C. destructans. C. destructans spore, only soil samples from the diseased ginseng farm produced the positive bands, suggesting its usefulness in detecting the C. destructans spores in soil samples. Thus it is recommended to first extract the whole genomic DNA from soil samples and use it for the PCR reaction, thereby eliminating the inhibitory activity of soil components.
Effects of Cutting Time and Scions Section in Root Cuttings of Lacquer Tree (Rhus verniciflua Stokes)
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 49~54
Effects of cutting time and scions section on shooting and plantlets growth characteristics were investigated to increase plantlets products by root cuttings in lacquer tree. Shoot induction from scion section was developed lately as it planted in earlier season. However, shooting rates were over 84% or more in cut planting on 15, 25 March and 5 April than sinee 15 April. Shoots were developed evenly as late cut planting. Generally, plant height, branch number, leaf number, stem diameter, and leaf area per plantlets was excellent in cut planting on 25 March and 5 April. Shooting rate from the root scion varied greatly by root sectionings. The rate of the 10 cm-long-scion was reached 84％, whereas only 54％ in no trimmed root scion after 10 weeks of cutting. Regenerated plantlets from root scion without cut were slightly good in shape, but their height, number of branch per plantlets, and stem diameter were not significant.
Variation of RAPD patterns between Male and Female Genomic DNAs in Dioecious Rumex acetosa L.
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 55~60
The genetic variation of random amplified polymorphic DNA (RAPD) patterns of genomic DNAs was investigated in dioecious plant Rumex acetosa L., which carries different sex chromosome complements in female (2n=12A+XX) and male (2n=12A+XY
). One hundred and twenty random primers consisted of 10-mer were used for PCR amplification. Polymorphic bands were found in 24 primers. Specific bands for female and male were 16 and 18, respectively. Especially, a band of 1,440 bp from the OPC-10 primer was male specific. These sex specific RAPD markers are used to understanding the sex determination mechanism in plants.
Taxonomic Studies of Korean Tripterospermum japonicum (Max.) Max. and Pterygocalyx volubilis Max.
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 61~73
External and anatomical characters such as stem, leaf, ovary, calyx lobe, ultrastructure of stigma epidermis of leaf blade and midvein, corolla lobe, seed coat and pollen were examined on Pterygocalyx volubilis and Tripterospermum japonicum of Korean Gentianaceae. Fruit characters(capsule and berry), absent or not of corona and pollen characters(reticulate and striate) has been used by identification characters of two taxa. But study results, external characters(root, leaf, calyx lobe, stigma, anther), internal characters(calyx love, ovary) and ultrastructure characters(epidermis o leaf blade and midvein, corolla lobe, seed coat) were useful for species identification. Taxonomic relationships among problematic taxa were discussed.
The Condition of Regeneration and Antibiotics Concentration for Gene Transformation of Zygotic Embryo in Panax ginseng C. A. Meyer
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 74~81
To obtain multi-shoot using zygotic embryos dissected from ginseng seed, the embryos were cultured on MS medium supplemented with CPA and BA. Effective multi-shoot induction was achieved on 0.5mg/ t CPA and 1.0mg/ t BA treatment. Among the various plant growth regulator treatment, MS basal medium with 1mg/ t 2,4-D and 0.5mg/ t kinetin was more competent and could be induced 4∼6 shoots per one embryo. Also, the best condition for pre-embryoid induction from ginseng cotyledon so as to ginseng transformation appeared to 1mg/ t 2,4-D and 0.5mg/ t kinetin treatment. The kanamycin level to select transformants varied greatly by different explant tyues. The petiole explants with leaf and embryo could survived up to 100
/ ml kanamycin concentration where as petiole explants without leaf died all at the level. Conclusionally, our results suggest that optimum kanamycin concentration for ginseng transformation using somatic embryos is about 75∼100
/ ml concentration.
Complete Nucleotide Sequence of Tobacco Mosaic Virus Isolated from Wasabi(Eutrema wasabi Maxim.)
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 82~88
Genomic RNA sequence of a tobamovirus infecting Eutrema wasabi plant(TMV-W) was determined. The RNA is composed 6,298 nucleotide and contains four OREs encoding the protein of 180KD(OREI), 130KD(ORE2),30KD(ORF3) and 18KD(coat protein, ORF4). ORE4, ORF 3, ORF 2 and ORF 1 are overlaped by 130, 20 and 40 nucleotides, and the overapping region can be folded into a stable hairpin styucture. This includes the 3'non-coding region of 238 nucleotides, coat protein gene(537 nucleotides,179 amino acid), 30KD movement protein gene(825 nucleotides, 275 amino acid), 13(IKD protein gene(1,896 nucleotides, 632 amino acid) and 180KD protein gene(2,958 nucleotides, 986 amino acid). The genomic RNA sequence was compared with homologous regions of eleven other tobamoviruses. TMV-WTE was similar to TMV-WSF(98.6%) in nucleotide sequence.
Pollen Morphology of the Genus Atractylis L. (Compositae)
Korean Journal of Plant Resources, volume 16, issue 1, 2003, Pages 89~98
Pollens of six taxa in the genus Atractytis L. confused with Atractylodes DC. were investigated by the light and the scanning electron microscope. Pollen grains of six taxa in this genus were monads, 46.5-66.7
in length of polar axis, 43.6-60.47
in equatorial width. Aperture was tri-colporate, 19.6-29.1
in colpus length,7.3-11.0
in colpus width and 6.4-10.6
in porus diameter. The exine was composed of three layers(foot layer, columellne, tectum), and 5.2-8.3
in thickness. The surface sculpturing pattern was echinate, the spines were 1.4-5.8
in length and 6-33 per 20
in distribution numbers over the surface. Atractylis L. was not distinguished from Atractylodes DC. by pollen morphology, especially the pollens of Atractylis arabica, A. aristata, A. carduus, A. microcephala were very similar to that of Atractylodes. But its taxonomic significance was different, the taxa of Atractylodes DC. were divided into 2 types by only pollen size, but the treated taxa in this study were divided into various types by pollen size, shape of equatorial view, surface sculpturing and spine size. The characteristics of spine size and shape of equatorial view in A. cancellata and A. prolifera were very useful for consideration of evolutionary trends in this genus.