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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 17, Issue 3 - Oct 2004
Volume 17, Issue 2 - Jun 2004
Volume 17, Issue 1 - Feb 2004
Selecting the target year
Effects of Cryopreservation on the Seed Germination and Growth Properties of Seedlings of Maackia amurensis
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 75~81
This study was conducted to investigate effects of cryopreservation by vitrification on the seed germination rate and growth and physiological properties of seedlings of Maackia amurensis. Cryopreservation significantly decreased the germination rate of seeds of M. amurensis, but the reduction of germination rate was mitigated by the treatment of cryoprotectant (plant vitrification solution, PVS2) before plugging into liquid nitrogen and fast thawing rate after cryopreservation. Long-term PVS2 exposure decreased seed germination rate, whereas cryopreservation time didn't have influence on seed germination rate. In addition, growth and physiological properties of seedlings were not affected by PVS2 exposing time and cryopreservation time. Therefore cryopreservation could be widely used as a technique of long-term ex situ conservation without any damage and deterioration of cells or tissues of the forest seeds. However, in order to increase the effect of cryopreservation, we have to develope the lower toxic cryoprotectant and suitable techniques to the structural or chemical properties of a variety of seeds.
A Phylogenetic Relationships of Araliaceae Based on PCR-RAPD and ITS Sequences
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 82~93
Phylogenetic relationships among species in Araliaceae were analyzed using PCR-RAPD and sequence of ITS region of nuclear ribosomal DNA based on samples collected in Korea. RAPD analysis showed various polymorphic bands which were able to differentiate species and genus, and specific bands showing variations among individuals within species. Cluster analysis using gel images revealed high molecular variability within species of Aralia eleta. No significant variation was found among cultivated species of Panax ginseng, but they showed high genetic differences with wild type of the species. In ITS analysis, specific sequences for each genus and species were observed and these were allowed to differentiate species and genus. Phylogenetic analysis using ITS sequences showed that Acanthopanax and Kalopanax had a close relationship, and Aralia and Panax are monophyletic, but genus Hedera is different species from other species in family Araliaceae in this study. The results showing close relationship between genera Aralia and Panax were also observed in RAPD analysis. Contrary to the results of RAPD analysis of Panax ginseng, sequence analysis of ITS showed no significant difference between wild mountain ginseng and cultivated species of P. ginseng. Also, both RAPD and ITS analysis of P. ginseng showed no significant genetic variability among cultivation sites. Results indicate that P. ginseng cultivating in Korea is monophyletic. The molecular analysis used in this study agreed on classification using morphological feature. These results suggest that molecular techniques used in this study could be useful for phylogenetic analysis of Araliaceae.
Effect of Benzyladenopurine Concentration on Growth and Morphology of Soybean Sprouts and Comparison with Selling Products
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 94~101
Commercial value of soybean sprouts should be affected by their morphology including the lateral roots. This study was carried out to determine the effect of benzyladenopurine (BA) concentration on growth and morphology of soybean sprouts in order to compare them with the selling products collected from the markets. Four cultivars (cv. Eunhakong, Pungsannamulkong, Sowonkong, and Junjery) were cultured for 6 days after imbibed for 5 hours into different BA solutions (0, 1,2,4, and 8 ppm). On the 6th day, hypocotyl length was measured to calculate the composition rate of ＞ 7 cm (A), 4 to 7 cm (B), ＜ 4 cm (C) and non-germination (D), and the ratio of hypocotyl to root length (H/R ratio) on the base of hypocotyl length as well as lateral root, hypocotyl diameter, and fraction fresh and dry weights. The composition rates classified with hypocotyl length showed big differences between 4 cultivars in class A and C; Class A, in which cv. Sowonkong and Junjery had higher rates than cv. Eunhakong and Pungsannamulkong, was decreased with BA concentration of higher than 4 ppm, but class C showed the reverse result to class A. Any lateral root was not formed in higher than 4 ppm BA solutions although fewer in cv. Pungsannamulkong and Junjery than in the two other cultivars. Hypocotyl and root lengths were decreased with increased BA concentrations, and in all BA concentration, hypocotyl length of cv. Junjery was the longest. Hypocotyl diameter, hypocotyl and total fresh weights were thicker and heavier in 2 to 4 ppm BA concentrations than in the other ones, although in case of BA application, cv, Eunhakong and Sowonkong were heavier. The H/R ratios were increased with increased BA concentration. The ratios of nearly all the selling products ranged from 1 to 2 ppm when compared to them from our experiment.
Effects of Planting Density of Wanggol (Cyperus iwasaki Makino) on Dry Cortex and Medulla Yield at Rice Field
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 102~106
To determine the optimal planting density of wanggol in southern areas of Korea, Gangsan early local, the highest yielding variety was grown under four different plant density. Yield components such as stem length, number of tiller, stem diameter were highest at the plots with 18 cm row spacing and 18 cm plant spacing. Dry cortex and medulla yield were highest at the plots of 18 cm row spacing and 18 cm plant spacing. Judging from the results reported above, at optimum planting density of wanggol seemed to be 18 cm row spacing and 18 cm plant spacing.
Development of Universal Primers for Phylogenetic Analysis and Species-specific Band Identification in the Genus Actinidia
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 107~115
To develop universal primers for phylogenetic analysis and species-specific marker for breeding program of kiwifruit, eighteens primers were designed from kiwifruit genome-specific repeat sequences. Seven species including twenty two varieties collected from native eastern Asia were examined using 18 to 22 mer kiwifruit target(KT) primers. among eighteen primers, we selected seven primers for phylogenetic relationship. The genus Actinidia was divided into two large groups; group I,A. arguta, A. melanandra, A. kolomikta, and A. marcrosperma, characterized by the non-hair in fruits and loaves or a few pubescences only in young stage, which belongs to the section Leiocarpae, and group II, A. chinensis, A. deliciosa, and A. eriantha, characterized by a lot of hairs only in young fruit stage and with a lot of hairs or fuzzes in leaves and branches, which belongs to the section Stellatae. Group II especially belongs to the series Perfectae of the section Stellatae and was divided into two subgroups; subgroup I containing A. chinensis and A. deliciosa, and subgroup II containing A. eriantha. In contrast, the two species, A. chinensis and A. deliciosa, which are known to have common parents, were divided into two independent subgroups with 80％ of a similarity value. On the other hand, we selected KT6F for variety specific bands, KT12E primers for 'Hayward' and 'Tomuri'. KT7F or KT12F primers were useful for analysis of inheritance pattern in kiwifruit cross-breeding. We suggest that these primers will be a powerful tool for elucidating phylogenetic relationship and selection of novelty kiwifruit in a breeding program.
Gene Analysis Related to Red-skin Disease of Ginseng by Molecular Marker
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 116~121
Panax ginseng discarded and lower than 4th grade is caused by red skin disease showing red color skin in ginseng. This kind of red skin ginseng is found a lot in Panax ginseng rather than Panax quinquefolium, and it is considered that red skin disease might be caused by gene. Therefore, this study was carried out to detect genes resistant to red skin disease using RT-PCR. RNA was extracted from three years old ginseng root of both red skin and normal portion in the same root. After RNA extraction, PCR amplification was performed from cDNA using many random primers. As a result, specific band for red skin was found. It is considered that the gene forming band has possibility to be related with red skin disease, and this gene should be decided if it's related with red skin disease. If that gene is related with red skin disease, it will be used for transformation to foster for resistance to red skin disease as well as for selection marker. Bowever, if it's not related with red skin disease, more primers should be used to find gene related with red skin disease.
Resource Plants of Mt. Midong in Chungcheongbuk-do, Korea
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 122~134
This study was carried out to investigate the resource plants of Mt. Midong. The resource plant of Mt. Midong was surveyed from March, 2002 to October, 2003. In the result of survey, the vascular plant species in Mt. Midong were summarized as 400 taxa; 85 families, 266 genera, 349 species, 48 varieties and 3 forms. And in the results of survey on resource plants among 400 taxa, we confirmed 162 taxa of ornamental plants(40.5％), 250 taxa of edible plants(62.5 ％), 257 taxa of medicinal plants(64.3％) and 167 taxa of the others(41.8 ％). The six taxa were recorded in the surveyed sites as the Korean endemic plants; Hytomecon hylomeconoides, Filipendula palmata var. glabra, Ajuga spectabilis, Weigela subsessilis, Cirsium setidens and Saussurea seoulensis. The rare and endangered plants designated by Korea Forestry Service were two taxa; Hylomecon hylomeconoides and Polygonatum stenophyllum.
Effects of Transplanting Time of Wanggol (cyperus iwassaki Makino) on Dry Cortex and Medulla Yield at Rice Field
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 135~138
To determine the optimal transplanting time of wanggol in southern areas of Korea, wanggol c.v. Gangsan the highest yielding variety was grown under three different tansplanting times. Yield components such as stem length, number of tillers, number of heading stem and stems diameters were the highest at the transplanting time of May 20. Plants sown of Mar 30 showed the highest dry cortex and medulla yield. Judging from the results reported above, at optimum transplanting time combined with sowing time toy yield of wanggol (cyperus iwassaki Makino) seemed to be the time of May 20 and Mar. 30.
Growth, Yield and Photosynthesis of Introduced Kenaf Cultivars in Korea
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 139~146
Kenaf (Hibiscus cannabinus L.), an annual plant of the family Malvaceae, is considered to be the most promising for alternative plants with potential use as a non-wood fiber source. The objectives of this study were to select the optimum seeding date and adaptable cultivar among newly introduced kenaf cultivars (Everglades-41, Tainung-2 and Chingpi-3) in Jeju island, and to clarify the photosynthetic characteristics of those cultivars. Among the three cultivars, the fresh shoot weight per unit area of Chingpi-3 at harvest season showed highest through all seeding dates, that of while Everglades-41 was the lowest. The difference of shoot yield at harvest mainly due to seedling stand rate and plant number per unit area among the cultivars. The Chingpi-3 showed the highest shoot fresh weight in the seeding date of May 11 and decreased trend in the late seeding dates. Net photosynthesis rate was higher on Everglades-41 with entire type leaf than other two cultivars with palmate type leaf. The activity of ribulose-1,5-bisphosphate carboxylase/foxygenase (rubisco) estimated from the A-Ci curve showed highest in Chingpi-3 among three cultivars. These results suggest that Chingpi-3 might be adaptable cultivar with seeding date of around May 10 for kenaf production in Jeiu island.
Studies on the Cropping System of Sesame(Sesamum indicum L.) of Korea
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 147~152
To increase the utilization of farming lard and the income of farmers four cropping systems by introducing two crops in the cultivation of sesame and perilla were tested from May, 2001 to June, 2002. Weed occurrence was observed abunantly at the in barley＋sesame cropping system. Among cropping systems, weed in barley+sesame was greater in post-crop than in pre-crop, but other cropping systems were in pre-crop. Pre- or post-crops at the barley＋sesame, sesame+chinese cabbage showed good growth. The income of cropping system containing sesame was higher 21％ and 61％ in barley＋sesame and sesame＋chinese cabbage, than in sesame and perilla mono-crop systems respectively. The sesame＋chinese cabbage was labor-saying and cost down cropping system, because polyethylene film of pre-crop was reused in post-crop. But when perilla was introduced as post-crop of sesame, the delayed seed time resulted in decreased growth and grain yield. Soil physical properties were improved at the sesame＋chinese cabbage and barley＋sesame cropping systems.
Comparative Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Glehnia littoralis Schmidt et Miquiel
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 153~160
Glehnia littoralis is known as an edible and medicinal plant using green loaves and mature roots of plant. In the present paper, the influence of plant growth regulators on callus induction and plant regeneration was investigated. Callus induction and regeneration occurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plant growth regulators for callus induction from leaf and petiole explants was MS basal medium supplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction was higher in petiole explant than leaf. When the callus was cultured on MS basal medium supplemented with 0∼1 mg/L IAA, 0∼1mg/L NAA and 0∼2mg/L BA for about 65 days, the most effective plant growth regulators on plant regeneration from callus were 1mg/L NAA and 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix.
In vitro Propagation of Transgenic Ginsengs Introduced with Ferritin Light Heavy Chain Gene through Single Embryo Culture
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 161~168
Optimal regeneration conditions of ginseng transformants were studied. It has been known that Ferritin Light Heavy Chain (FLHC) gene remove the several heavy metal by combination, store and transport. To obtain the ginseng tolerant to heavy metal, binary vector was introduced in Agrobacterium by tri-parental mating and then Agrobacterium tumefaciens MP90/FLHC was selected on the AB media and MS media containing kanamycin. Explants were co-cultured with Agrobacterium tumefaciens MP90/FLHC, which contained NPT II as a selectable marker, tadpole ferritin heavy chain (FLHC) gene and human ferritin light chain gene and then a number of embryos were induced. The induced embryo transferred to shooting media consisting of MS medium supplemented with GA 10 mg/L. As a result of examination that induced the normal growth of transfomants, transformants showed the equivalent growth in both root and shoot on the media containing the 1/3 MS.
The Flora of Mt. Bakdal in Chungcheongbuk-do, Korea
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 169~182
The purpose of this study is carried out accurately to grasp the vascular plants in Mt. Bakdal (825 m of sea level), Goesan-gun, Chungcheongbuk-do. In the results of survey from October, 2000 to August, 2001, the vascular plant species in Mt. Bakdal were summarized as 458 taxa; 91 families, 275 genera, 391 species, 62 varieties and 5 forms. The rare plants designated by Korea Forestry Service were six species; Lilium distichum, Iris uniflora var. carisina, Hylomecon hylomeconoides, Viola albida, Scopolia japonicus and Scrophularia koraiensis. And the endemic plants were seventeen species; Melandryum seoulense, Aconitum pseudo-laeve var. erectum, Hylomecon hylomeconoides, Spiraea prunifolia for. simpliciflora, Rubus hongnoensis, Gleditsia japonica var. koraiensis, Lespedeza
tomentella, Vicia venosa var. cuspidata, Vicia venosissima, Tilia taquetii, Ajuga spectabilis, Paulownia coreana, Scrophularia koraiensis, Weigela subsessilis, Lonicera subsessilis, Cirsium setidens and Saussurea seoulensis. The sixteen species were recorded in the surveyed sites as the naturalized plants; Rumex acetocella, Rumex crispus, Fallopia dumetora, Chenopodium glaucum, Amaranthus retroflexus, Robinia pseudo-acacia, Amorpha fruticosa, Trifolium repens, Abutilon avicennae, Hibiscus trionum, Oenothera odorata, Oenothera lamarckiana, Erigeron annuus, Erigeron canadensis, Bidens frondosa and Carduus crispus.
Growth and Yield by the Different Seeding Methods and Cultivating Root Weight in Atractylodes macrocephala Koidz
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 183~188
This study was conducted to investigate the effects on the growth and yield by the different seeding methods and cultivating root weight in Atractylodes macrocephata Koidz. Seeding distances were different such as 5
5 cm, 5
10 cm, 10
10 cm, 10
15 cm by the hand seeder and 15 cm seeding in drill. Emergence date, plant height were not significantly changed with seeding space, but the number of leaf, fresh weight of above-ground part and fresh weight of root were increased in the sparse seeding compared with the dense seeding. The highest fresh root yield was 1,012 kg/10 a at the 5
5 cm. Roots yield was increased in the sparse seeding compared with the dense seeding, but the highest number of roots above 16 g yield was observed at the 10
10 cm seeding distance. Emergence date was faster 1∼3 day root weight above 6 g than that root weight 5 g. The plant height, number of stem and fresh weight of above-ground part were more increased as the root weight was heavier. The growth of underground part were more increased as the root weight was heavier, yield was increased about 27％ to 112％ compared with root weight above 6 g than that 134.6 kg/10 a with root 5 g.
Interspecific Similarity of the Subgenus Diploxylon in Korea Based on Pollen Morphological Characters
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 189~201
The present study has measured eight pollen morphological parameters of Diploxylon species in Korea by light microscopy (LM). The results are as follows; 1. Diploxylon species in Korea showed significant (P＜0.01) interspecific difference in their pollen morphological parameters. 2. The discriminant analysis based on the pollen morphological parameters demonstrated that the classification ratio of Diploxylon was 49.9％. The maximum was at Pinus banksiana (72.8％) and the minimum was at P. sylvestris (62.2％). 3. The relationship among the Diploxylon species based on their pollen morphological parameters showed that P. densiflora and P. sylvestris were had the closest relationship while P. rigida and banksiana had the least relationship.
Interspecific Similarity of the Subgenus Haploxylon in Korea Based on Pollen Morphological Characters
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 202~212
The present study was conducted to compare of pollen morphological characteristics for five Haploxylon species in Korea using light microscopy(LM). The results are as follows; 1. Highly significant (P＜0.01) interspecific difference was observed in five Haploxylon species for their pollen morphological parameters. 2. The discreminant analysis based on the pollen morphological parameters demonstrated that the classification ratio of Haploxylon was 68.8 ％ ranging from 72.8 ％ of Pinus pumila to 62.2 ％ P. koraiensis. 3. The relationship among the species based on their pollen morphological parameters showed that P. koraiensis and P. pumila in Haploxylon were most closely related while P. pumila and P. bungeana were least related.
Characteristics of Local Perilla Collected in Kangwon province of Korea
Korean Journal of Plant Resources, volume 17, issue 2, 2004, Pages 213~218
This study was conducted to examine the variations in 42 local perilla collected in Kangwon province of Korea and to provide basic informations which can be utilize in perilla breeding programs. The results obtained were summarized as the follows. Maturing period was widely distributed from 109 to 140 days. The maturing periods of most collections were 116 to 130 days(95.2％). and that 4.8％ of collections shown maturing period of below 115 days and above 131 days. Three varieties 109 day of maturing period were selected among local perilla collected from Yanggu gun. Stem length was ranged from 80 to 140 cm. The stem length of 111 to 130 cm, below 111 cm, and above 131 cm were 54.8％, 38.6％, and 16.7％, respectively. The number of cluster per plant was distributed 23 to 120. The distribution of cluster numbers were 40 to 70, under 40, and above 100 was 57.2％, 16.7％, and 11.9％, respectively. The number of seed capsules was distributed 20 to 40 and the collections ranged from at of 30 to 35 was 38.1 ％. Seed cluster length was distributed from 5.1 to 9.0 cm and that ranged from 6.1 to 8.0 cm was 64.3％, 1,000 grain weight ranged from 1.7 to 3.8 g. The distribution of 1,000 grain weight is 2.4 to 2.6 g, 3.0 to 3.2 g, and below 2.9 g was 35.7％, 19.1％, respectively. The 1,000 grain most weight 3.8 g was selected among local perilla collected in Hwacheon gun.