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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 19, Issue 6 - Dec 2006
Volume 19, Issue 5 - Oct 2006
Volume 19, Issue 4 - Aug 2006
Volume 19, Issue 3 - Jun 2006
Volume 19, Issue 2 - Apr 2006
Volume 19, Issue 1 - Feb 2006
Selecting the target year
Antioxidant and Hepatoprotective Effects of Tomato Extracts
Rhim, Tae-Jin ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 649~654
The objective of present study was to investigate the anti oxidative and hepatoprotective effects of tomato extracts. Total antioxidant capacity and total antioxidant response were 5.5 and
Trolox equivalent per mg of tomato extract, respectively. DPPH radical scavenging activity of tomato extracts (
) was 70% as compared to 100% by pyrogallol solution as a reference. The effect of the tomato extracts on lipid peroxidation was examined using rat liver mitochondria induced by iron/ascorbate. Tomato extracts at the concentration of
significantly decreased TBARS concentration. Tomato extracts prevented lipid peroxidation in a dose-dependent manner. The effect of the tomato extracts on reactive oxygen species (ROS) generation was examined using cell-free system induced by
. Addition of
of tomato extracts significantly reduced dichlorofluorescein (DCF) fluorescence. Tomato extracts caused concentration-dependent attenuation of the increase in DCF fluorescence, indicating that tomato extracts significantly prevented ROS generation in vitro. The effect of tomato extracts on cell viability and proliferation was examined using hepatocyte culture. Primary cultures of rat hepatocytes were incubated with 1mM tert-butyl hydroperoxide (t-BHP) for 90 min in the presence or absence of tomato extracts. MTT values by addition of tomato extracts at the concentration of 2, 10, and
in the presence of t-BHP were 13, 33 and 48%, respectively, compared to 100% as control. Tomato extracts increased cell viability in a dose-dependent manner. These results demonstrate that tomato extracts suppressed lipid peroxidation and t-BHP-induced hepatotoxicity and scavenged ROS generation. Thus antioxidant and hepatoprotective effects of tomato extracts seem to be due to, at least in part, the prevention from free radicals-induced oxidation, followed by inhibition of lipid peroxidation.
Isolation of Dineolignans, Saucernetin-7 and -8, with Nitric Oxide Inhibitory Activity and NMR assignment from Saururus chinensis
Lee, Kyung-Tae ; Jung, Hyun-Ju ; Park, Hee-Juhn ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 655~659
Two dineolignans (1,2) with nitric oxide inhibitory activities were isolated from Saururus chinensis (Saururaceae) using silica gel column chromatography. Although the structures, saucernetin-7 (1) and -8 (2), have been already reported, NMR assignment of the two compounds was completed aided by 2D-NMR spectroscopy including
COSY, HMBC and NOESY NMR spectra. Compounds 1 and 2 exhibited significant nitride oxide inhibitory activity in LPS-induced RAW 264.7 cells with
Antinociceptive and Antiinflammatory Effect of a Diterpene Isolated from the Aerial Part of Siegesbeckia pubescens
Park, Hee-Juhn ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 660~664
The aerial part of Siegesbeckia pubescens (Compositae) has been used to treat rheumatoid arthritis and hypertension in the Oriental medicine. This crude drug has been used without process (SP-0) or with three times-process of steaming and drying (SP-3) or the nine times of that process (SP-9). To search for the antinociceptive anti-inflammatory components from this crude drug, activity-directed fractionation was performed on this crude drug. Since the
extract was shown to have a more potent effect than other extracts, it was subjected to silica gel & ODS column chromatography to yield two diterpene compounds (1). Compound 1 was structurally identified as ent-16 (H, 17-hydroxykauran-19-oic acid, which were tentatively named siegeskaurolic acid A. A main diterpene, siegeskaurolic acid A was tested for the antiiflammatory antinociceptive effects using both hot plate- and writhing anti-nociceptive assays and carrageenan-induced anti-inflammatory assays in mice and rats. Pretreatment with siegeskaurolic acid A (20 and 30mg/kg) significantly reduced the stretching episodes, action time of mice and carrageenan-induced edema. These results support that siegeskaurolic acid is a main diterpene responsible for antinociceptive and antiiflammatory action of S. pubescens. In addition, the assays on SP-0, SP-3 and SP-9 produced the experimental results that SP-9 had more significant effects than other two crude drugs. These results suggest that the processing on the original plant may lead to the higher pharmacological effect.
Cryopreservation of Somatic Embryos of Soapbeny (Sapindus mukorossi Gaertn.) by Vitrification
Kim, Hyun-Tae ; Yang, Byeong-Hoon ; Park, Young-Goo ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 665~669
Somatic embryos do not survive at exposure to liquid nitrogen temperatures without cryoprotective treatments. A simplified technique which simultaneously induces and cryoprotects embryogenic calli using plant vitrification solution 2 (PVS2) followed by dehydration was developed for the cryopreservation of Soap berry genetic resources. Vitrification is a way of removing the moisture in vegetation through PVS2. The PVS2 vitrification solution consisted of 30% glycerol (w/v), 15% ethylene glycol (w/v), 15% Dimethylsulfoxide (w/v) in B5 medium containing 0.4M sucrose. Two tests were done. The one was to eliminate moisture at
and the other at
. In both cases the best results came out at a vitrification time of
minutes. It was also found that the survival rate was higher at
. In particular, the survival rate reached more than 80%. Water-damaged embryos turned brown and stoped growth, but energetic embryos took on a milky hue and show a very vigorous growth rate. Successful cryopreservation of somatic embryos of soapberry can be used to establish in vitro genebanks for long-term conservation of Soapberry genetic resources to complement field genebanks and other in vitro methods already being used.
Antioxidant and Hypoglycemic Activity of Polysaccharide from Tea
Yu, Zhi ; Zhang, Yun ; Ni, De-Jiang ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 670~676
Tea polysaccharide had high antioxidant activity and it could be used to cure diabetes. Antioxidant activity of tea poly-saccharide (TPS) from three kinds of tea (green tea, oolong tea and black tea) were compared, the result indicated that oolong tea polysaccharide (OTPS) had the highest antioxidant activity. In order to explicate the mechanism of antioxidant and hypoglycemic activity, the streptozotocin (STZ)-induced diabetes mice model (DM) was established. The influence of OTPS on blood-glucose, content of MDA and NO, and activities of GSH-PX, SOD, NOS in serum, kidney and liver were investigated. The result showed that after four weeks injection of OTPS to DM mice, the blood-glucose of three treatment group reduced by 14.5%,21.5% and 33.3%, respectively, comparing to the model control. The reduction effect of OTPS increased with the rise of dose. The activity of SOD and GSH-PX elevated significantly, while the activity of NOS decreased. The content of MDA and NO reduced significantly. The above results imply that antioxidant activity was enhanced. Comparing to XKW treatment, the effect of a dose of 300mg/(kg. bw) OTPS was much better. The research showed that the OTPS had a significant effect on reducing blood glucose, and could enhance the antioxidant activity of DM mice.
Heritability and Genetic Gains for Height Growth in 20-year-Old Korean White Pine in Korea
Shin, Man-Yong ; Park, Hyung-Soon ; Cho, Yoon-Jin ; Chung, Dong-Jun ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 677~679
The objectives of this study were to examine the genetic variation of 20-year-old tree height and to estimate heritabilities and genetic gains of Korean white pine. Analysis of variance showed that families and family x block interaction had the significant (p=0.01) effects on tree height. However, family variation appears to be much greater than the variation due to family x block interaction. Individual tree heritability was higher (
) than family heritability, (
) therefore, combined selection showed the largest genetic gain (17.76%) in a given equal intensity of selection.
Pre-Sowing Seed Treatment: Effect of Deep Sea Water Priming on Germination of Wild Vegetables; Gondalbi (Cirsium setidens), Jandae (Adenophora triphylla var. Joponica Hara) and Deoduck (Codonopsis lanceolata Trautv.)
Yoon Byeong-Sung ; Shrestha Surendra Lal ; Kang Won-Hee ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 680~686
This experiment was conducted on wild vegetables; Gondalbi (Cirsium setidens), Deoduck (Codonopsis lanceolata Trautv.), and Jandae (Adenophora triphylla var. Joponica Hara) seed to study whether priming with deep sea water results in enhancement of seed germination and identify the optimum concentration of the priming solution, and duration of priming. Seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) of deep sea water (DSW) in 12 hours, 24 hours, and 48 hours at
. Since Jandae had seed dormancy, it was kept for four weeks in refrigerator at
after priming treatment. In Deoduck, 5 percentage DSW priming significantly improved the early germination percentage, radicle length, and plumule emergence percentage. Among the priming period of treatments, 24 hours priming showed better performance in this treatment whereas, in Jandae, 12 hours priming with 10 percentages DSW significantly improved the germination percentage and germination rate. This treatment had increased the final germination percentage by 54%, 15% and 40% compared with control, plain water and
priming respectively. But in Gondalbi, priming did not improve the germination of seed. However, among the priming treatments, 12 hours priming with 3%
and 20% DSW gave better performance. In both the wild vegetables; Deoduck and Jandae, priming in deep sea water had improved the germination percentage and germination rate as compare to plain water,
, and without priming treatment. Hence the best seed priming treatment on Deoduck and Jandae are 24 hours with 5% DSW and 12 hours with 10% DSW respectively.
Effect of Deep Sea Water Seed Priming on the Growth of Rice (Oryza sativa L.) Seedlings
Yoon Byeong-Sung ; Shrestha Surendra Lal ; Kang Won-Hee ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 687~691
This experiment was conducted on rice (cv. 2005 Thaoi) seeds to study whether priming with deep sea water (DSW) results in enhancement of seed emergence and seedling growth and to identify the optimum concentration of Deep Sea Water (DSW) for priming. Two experiments were conducted subsequently. In experiment 1, four concentrations of the DSW (10%, 20%, 30% and 40%), and in experiment 2, five concentrations of DSW (10%, 15%, 20%, 25% and 30%) were prepared and seeds were primed for 24 hours duration at
. Beside this, hydro priming with plain water was also included as a control. Experiments were laid out in Completely Randomized Design (CRD) with three replications. Result showed that 20% DSW seed priming treatment had improved the emergence, seedling height, number of roots and root length as compare to other with DSW or without DSW treatments. Beyond 20% DSW priming (i.e. 25%, 30% and 40%) were not suitable for priming the seed. On the basis of seedlings growth parameters; emergence, seedling height, root number and length, and shoot root ratio, 20% DSW priming was the best priming treatment.
Screening of Seed Disinfectant for Controlling Brown Leaf Blight in Alisma plantago Double Cropping after Early Rice
Shin, Dong-Young ; Kwon, Byung-Sun ; Lim, June-Taeg ; Hyun, Kyu-Hwan ; Lim, Jung-Mook ; Seo, Young-Nam ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 692~696
This study was conducted to evaluate the effect of seed disinfectant, in control of brown leaf blight, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. Experimental plot was laid out in split plots design with three replications. The major seed disinfectants were benomyl Wp, 20%, Captan Wp, 50%, Triferine Ec, 17%, Etridia zole Ec, 25%, and Thioplant-mythyl Wp, 50%. Even though seed disinfectant treated had no effect on the growth and flowering date of Alisma plantago, dry root yield was increased largely with benomyl Wp, 20%, in seed disinfectant than in the other seed disinfectants and contorl. All seed disinfectants had no injury with standard dosage. But all seed disinfectants had slight injury in the double dosage level for the Alisma plantago. On the basis of yield, vegetative and disease paramerer, benomyl Wp (20%) (
) had shown superior performance, however, all the seed disinfectants are effective as compare to without treatment.
Effect of Complex Fertilizer on Yield and Yield Components of Rapeseed
Shin, Dong-Young ; Kwon, Byung-Sun ; Lim, June-Taeg ; Jung, Dong-Soo ; Hyun, Kyu-Hwan ; Lim, Jung-Mook ; Seo, Young-Nam ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 697~699
To find out the best complex fertilizer for high yielding of rapeseed crop, experiment was conducted on complex fertilizers at the experiment field in upland of rapeseed in Mokpo Experiment Station, Nat'l Institute of Corp Science, RDA, Korea. Experiment was laid out in randomized complete block (RCBD) design. The effects of complex fertilizer (22-22-11) on the number of branches, pod length, percentage of seed set and seed yield were highest but on the plant height, ear length, and number of pods per ear were negligible. On th basis of the results reported above, for getting higher yield of rapeseed crop, among the tested fertilizers complex fertilizer (22-22-11) gave the superior performance and is recommended for application.
Effect of Temperature on Seed Germination of Korean Native Viola Species
Lee, Cheol-Hee ; Hwang, Ju-Kwang ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 700~705
Present studies were performed to determine the physiology of seed germination in Viola species native to Korea. Twelve species, 1 variety and 1 form were collected, classified and used as materials: V. collina, V. blandaefomis, V. rosii, V. chaerophylloides, V. phalacrocarpa, V. patrinii, V. mandshurica, V. mandshurica for. albescence, V. seoulensis, V. yedoensis, V. keiskei, V. variegata, V. variegata var. chinensis, and V. verecunda. V. tricolor 'Helen Mount' was also used to compare wild with cultivated species. In order to investigate the effect of temperature on seed germination, seeds stored at
for 10 months or 4 years were incubated at 10, 15, 20,
under 16h illumination with 4 replicates per treatment. Seeds which had not germinated at
were transferred to
to assess the effect of temperature change in germination. Germination percent and the days of first, 40% and 80% germination were assessed. Capability of seed germination varied with taxon; Species belonging to subsection Patellares had high ability of germination, compared to species in the other subsections, and series Chinensis was the best among subsection Patellares. Species capable of high germination germinated in all temperatures with reasonably high germination rate, but the other species responded sensitively to temperature with different germination patterns. Higher the temperature, shorter the incubation time required for first, 40% and 80% of germination. Therefore, high temperature was effective in almost all species, not only for inducing high rate of germination but also the uniformity of germination. Temperature change from
had a positive effect on seed germination.
Production and Characterization of Nitrate Reductase Deficient Mutants in Petunia parviflora
Lee, Cheol-Hee ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 706~715
Nitrate reductase deficient (NR) mutant lines were selected indirectly by their resistance to 100mM chlorate in cell cultures of P. parviflora. A total of 585 chlorate resistant lines were confirmed by a second passage on a high concentration of chlorate. Frequency of spontaneous mutation was
in 3 month old suspension-cultured cells, and in non-selective media containing amino acids as sole nitrogen source. The frequency of mutation could be increased up to 11-fold by culture for 12 months. Out of 40 randomly selected calli, 22 were fully deficient in NR. The rest of the clones contained a decreased level of NR activity. Further characterization was carried out in 13 mutant lines which were fully deficient in NR and in 5 mutant lines containing residual (0-7.0%) NR activity, as compared to wild-type cells cultured on the same medium. The
mutants were tentatively classified as defective in the NR apoenzyme (nia-type; 11 mutant lines including the 5 with residual NR activity) or in the molybdenum cofactor (cnx-type; 7 mutant lines) by the XDH activity. The cnx-type could be further classified into two groups. In one group (5 mutant lines) of these, the NR activity could be partially restored by nonphysiologically high (1.0mM) molybdate in the culture medium. Both types of
mutants were unable to grow on minimal medium containing nitrate as sole nitrogen source, but grew well on amino acids. They also proved to be extremely sensitive to the standard medium (
) containing nitrate and ammonium. Shoot regeneration was obtained only in the
mutants, which contained residual NR activity, but they so far have failed to grow into plants.
Evaluation of Genetic Diversity among the Genus Viola by RAPD Markers
Oh, Boung-Jun ; Ko, Moon-Kyung ; Lee, Cheol-Hee ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 716~720
The genetic diversity among the genus Viola was evaluated using the random amplified polymorphic DNA (RAPD) method. A total of 142 distinct amplification fragments by 18 random primers were scored to perform the cluster analysis with UPGMA. Viola species from the subsection Patellares were clustered into group I to IV. The groups from I to IV were consistent with its morphological taxonomy, series Pinnatae, Chinensis, Variegatae, and Patellares in the subsection Patellares, respectively. Even though V. albida and V. albida var. takahasii were classified in Chinensis, they were assigned into group I. The cluster analysis separated other subsections from Patellares in the section Nomimium. Interestingly, V. verecunda and V. grypoceras in subsections Biobatae and Trigonocarpae, respectively, were clustered into group C with a high similarity coefficient. Therefore, RAPD analysis can be used for providing an alternative classification system to identify genotypes and morphological characters of Viola species.
Eco-frendly Control of Culex pipiens (mosquito) Larvae by Acorus calamus (sweet flag) and Acorus gramineus (Grassy-leaved sweet flag) Extracts
Choi, Jeong-Keun ; Lee, Ji-Yeon ; Lee, Ja-Hyun ; Lee, Seong-Gene ; Han, Yeon-Soo ; Han, Tae-Ho ;
Korean Journal of Plant Resources, volume 19, issue 6, 2006, Pages 721~726
Mosquitoes are carriers of malaria and encephalitis. This study performed for eco-friendly control of mosquitos by using genus Acorus. Several solvents were used for the extraction of genus Acorus; water, ethanol, and methanol. Grinded leaves and roots were also included. Acorus extracts killed mosquito larvae and the ethanol extract showed the best result. Autoclaved Acorus water needed long time to kill mosquito larvae.
of 1 % Acorus calamus decoction was 13.6 hrs and 1 % autoclaved Acorus water was 53.6 hrs.
of 0.05% Acorus calamus rhizome powder was 28.5 hrs.
of 0.5% Acorus calamus leaf powder was 10.8 hrs.
of 0.1 % Acorus calamus decoction was 63.4 hrs and 0.1 % Acorus calamus ethanol extracts was 48.6 hrs and 0.1% Acorus calamus methanol extracts was 53.9 hrs.
of 0.4% Acorus gramineus decoction was 45.5 hrs, 0.4% ethanol extracts was 10.9 hrs, 0.4% methanol extracts was 10.2 hrs.
of ethanol extracts was shorter than other extracts. Acorus calamus rhizome powder could be used for the eco-friendly control of the mosquito larvae.