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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 20, Issue 6 - Dec 2007
Volume 20, Issue 5 - Oct 2007
Volume 20, Issue 4 - Aug 2007
Volume 20, Issue 3 - Jun 2007
Volume 20, Issue 2 - Apr 2007
Volume 20, Issue 1 - Feb 2007
Selecting the target year
Comparison of Gene Coding Clones Content in In vivo and In vitro Methyl-Filtration Libraries of Maize(Zea may)
Lee, Myung-Chul ; Wing, Rod A ; Suh, Seok-Cheol ; Eun, Moo-Young ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 491~498
It has been hypothesized that efficient exclusion of methylated retrotransposons and repeated DNA region is one of the rapid and cost-effective approaches for comprehensive gene discovery in large genome size of maize. Three kinds of methylation-sensitive restriction enzymes, HapII, MspI and McrBC, were used to identify the restriction frequency of cytosine methylation sites in maize genome. Roughly 60% of total maize genomic DNA was restricted less than 500bp by McrBC, and the most of restricted small size fraction was composed retrotransposon. In order to validate the efficient construction of gene-rich shotgun library, we compare two gene-rich methyl-filtration shotgun libraries using in vivo and in vitro methyl-filtration system. The size selected DNA fraction by Sau3A-McrBC enzyme treated was very stable and has not appeared modification in E. coli, but most insert DNA size of partially digested with Sau3A were decrease less than 500bp by bacterial methylation-modification system. In compare of retroelements portion, A 44.6% of the sequences were retroelement in unmethyl-filtered library, and the most of them was Copia type, such as Prem, Opie and Ji. The portion of retroelement was drastically decreased to 25% and 20% by in vivo and in vitro filtration system, respectively.
In Vitro Assay on Antioxidant Activity and Cytotoxicity of Methanol Extracts from Young Sprouts of Several Korean Salad Plants
Chon, Sang-Uk ; Ahn, Chan-Young ; Lee, Sook-Young ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 499~503
Antioxidant activity, total phenolics level and cytotoxicity of the methanol extracts from the young sprouts of 5 Korean woody salad plants were determined. Methanol extracts of Kalopanax pictus had the highest DPPH radical scavenging activity, with an
value of 23.5mg
, and followed by Valeriana fauriei(43.1mg
), and Morus alba(>100mg
). Total phenolic content showed the highest amount in methanol extracts from Kalopanax pictus(23.7mg
), and followed by Valeriana fauriei(22.7mg
), Aralia elata(16.8mg
) and Morus alba(14.2mg
). In a MTT assay, methanol extracts of Aralia elata with
values of 151.0 and
showed the most potent cytotoxicity on Calu-6 and MCF-7, respectively. On the other hand, methanol extracts of Kalopanax pictus(
) showed the highest activity against HCT-116, and followed by those of Aralia elata(
), and Actinidia arguta(
). Total phenolic content of the tested plant extracts was correlated with the DPPH radical scavenging activity, suggesting the phenolic compounds may contribute to the antioxidant properties of Korean salad plants.
Genotype-Environment Interaction and Stability Analysis for Yield and Yield Contributing Characters in Soybean(Glycine max L.)
Islam, Mohammad Saiful ; Newaz, Muhammad Ali ; Islam, Md. Jahidul ; Heo, Seong-Il ; Wang, Myeong-Hyeon ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 504~510
GE interaction is the expression of differential genotypic adaptation across environments. GE interactions through different stability parameters and performance of the traits of genotypes were studied. The traits were days to maturity, pod length, number of pods/ plant, 100-seed weight and seed yield/plant in ten soybean genotypes across five environments. Significant differences were observed for genotypes, environments and GE interactions. Stability analysis after Eberhart and Russell's model suggested that the genotypes used in this study were all more or less responsive to environmental changes. Most of the genotypes perform better in Env.3. Based on phenotypic indices(Pi), regression (
) genotype Garurab was found fairly stable for days to maturity. BS-23 and G-2120 may be considered as stable genotype for pod length. All the genotypes except G-2120 showed that the genotypes were relatively unstable under environmental fluctuation for the number of pod/plant. Genotype BS-23 was found most stable among all the genotypes for 100-seed weight. BS-3 and Gaurab was the most stable and desirable genotypes for seed yield in soybean.
Strain Distinction and Their Distribution of Barley Yellow Mosaic Virus Base on RAPD Analysis in Korea
Park, Jong-Chul ; Rho, Tae-Whan ; Kim, Jung-Gon ; Kim, Hyung-Moo ; So, In-Young ; Lee, Kui-Jae ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 511~517
A stable method for strain distinction using viral RNA 1 structures analyses was developed and compared with the combined RT-PCR and RAPD methods. Seven out of 61 random primers were found to be polymorphic based on RAPD analysis resulting on the differentiation of the 33 BaYMV isolates into four distinct groups according to geographical districts. The first and largest group includes 13 isolate and consists mainly of two-rowed malting barley in Haenam area. The second group had ten collections from inland in west southern. The third group had seven isolates from west southern coastal region, where mainly six-rowed naked barley is cultivated. The last fourth group included three isolates from Gyungnam region in east southern area. Conclusively, RNA 1 analysis proved to be stable and efficient method for strain distinction for Korean BaYMV isolates. Further, results of pathogenicity and RNA 1 structure analyses revealed four groups BaYMV strains and were distributed all over Korea, represented by Naju, Haenam-okcheon, Iksan and Milyang.
The Anticancer Properties of Lunasin Peptide from Aged Callus Induced by the Soybean Tissue Culture
Park, Jae-Ho ; Jeong, Jin-Boo ; De Lumen, Ben O. ; Jeong, Hyung-Jin ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 518~523
Lunasin is small subunit peptide of coded from Gm2S-1 gene in soybean. It has been previously demonstrated that lunasin is a novel and promising cancer preventive peptide. Lunasin peptide is found only in the seed and not other tissues. And lunasin peptide starts to appear at 5 weeks after flowering and remains in the mature seed. We report here firstly lunasin peptide identified from soybean callus induced by the tissue culture and demonstrate its anticancer properties. The lunasin was identified and purified from soybean callus aged for 6 months. The callus lunasin(
) inhibited the acetylation of histone H3 and H4 by 58.8% and 56.5%, respectively. And it fully inhibited foci formation compared to the values of the positive control(no lunasin) and negative control(no MCA). Purified lunasin was able to internalize into the cell and localized in the nucleus.
The Function of ArgE Gene in Transgenic Rice Plants
Guo, Jia ; Seong, Eun-Soo ; Cho, Joon-Hyeong ; Wang, Myeong-Hyeon ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 524~529
We carried out to study the function of ArgE in transgenic rice plants, which were confirmed by PCR analysis and hygromycin selection. Transgenic rice plants were with selectable marker gene(HPT) inserted in genome of the rice. Southern analysis with hpt probe confirmed by two restriction enzymes that copy numbers of the selectable gene was introduced into the plant genome. We displayed that the relationship between drought stress and ArgE gene with the overexpressing rice plants. From this result, we observed that the degree of leaves damage has no difference in control and transgenic lines. The total RNAs were extracted from 6 weeks-seedling in normal condition in order to examine their expression levels with ArgE-overexpressed transgenic rice. In particular, expression patterns of genes encoding enzymes involved in abiotic stress, including drought and salt stresses. OsGF14a and OsSalt were investigated by reverse transcription-PCR(RT-PCR). Expression levels of the OsSalt gene decreased significantly in transgenic rice plants compared to control plant. However, ion leakage measurement did not demonstrate any leaves damage change between control and ArgE transgenic plants exposure to mannitol treatment. These results suggest that expression of the ArgE is not involved in tolerance for drought stress in rice but may playa role of signaling networks for salt-induced genes.
Genetic Diversity and Spatial Structure of Symplocarpus renifolius on Mt. Cheonma, Korea
Jeong, Ji-Hee ; Park, Yu-Jin ; Kim, Zin-Suh ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 530~539
Genetic variation and structure of 9 subpopulations of Symplocarpus renifolius Schott ex Tzvelev on Mt. Cheonma, in Korea, were determined via starch-gel electrophoresis. The genetic diversity at 10 loci for 8 isozymes (
) was found to be considerably higher than that seen in other long-lived perennial plants. On the whole, the genotype frequencies were in accordance with Hardy-Weinberg expectations. Approximately 5%(
) of the total variability was among subpopulations. The high levels of observed genetic diversity in S. renifolius were attributed to a universal outcrossing system and other specific factors like differences in age classes and widely scattered individuals around the main distribution. Heterozygosity was highest at a mid-range of elevation(
). The lowest heterozygosity at lower elevation was attributed to the possible origin of seeds transported by water from upstream regions during the monsoon season. Spatial structure in a subpopulation evidenced a strong autocorrelation between closer individuals within
of distance. This was assumed to be attributable to the restricted seed dispersal characteristics of S. renifolius. In accordance with the findings generated in this study, some implications regarding the conservation of S. renifolius at the Mt. Cheonma were also presented.
Surveys of Vegetation in the Peninsular Geography of Youngweol
Yun, Sei-Young ; Shin, Young-Seob ; Yun, Min-Young ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 540~543
We have investigated the vegetation in the peninsula located in Ongjeong-Li Seomyeon in Youngweol-gun. Since this is a limestone area, boxtrees, Abelia mosanensis T. H. Chung ex Nakai, and Selaginella stauntoniana Spring were abundant, as these are types of vegetation characteristic of limestone areas. Pinus densiflora S. et Z. was the most dominant woody plant, but vegetational changes, such as the dominance of Quercus variabilis Blume, Quercus dentata Thunb, and Quercus mongolica Fischer, were observed in spots. In particular, the growth of nursery plants of pinus densiflora S. et Z. was not observed, and young trees like Quercus mongolica Fischer grow as low vegetation of Pinus densiflora S. et Z.. Thus, the ecological succession of Quercus mongolica Fischer is predicted. Outside the investigation area, as herbaceous plants, various vegetations were observed, including Selaginella stauntoniana Spring, Thalictrum coreanum H. Lev., Mukdenia rossii(Oliv.) Koidz., Platanthera freynii Kraenzl., Cephalanthera longibracteata Blume, Potentilla dickinsii Franch., Patrinia rupestris(Pall.) Juss., Swertia pseudochinensis H. Hara, Vicia venosa(Willd.) Maxim., Pyrola japonica Klenze ex Alef., Disporum smilacinum A. Gray, Artemisia stolonifera(Maxim.) Kom. for. Stolonifera, Smilax nipponica Miq., Adenophora triphylla var. japonica(Regel) H. Hara, Isodon inflexus(Thunb.) Kudo, Gentiana scabra Bunge for. Scabra, Polygonatum odoratum var. pluriflorum(Miq.) Ohwi, Dioscorea quinqueloba Thunb., Syneilesis palmata(Thunb.) Maxim., Asparagus schoberioides Kunth, Eupatorium japonicum Thunb. ex Murray, Galium kinuta Nakai & Hara, Saxifraga fortunei var. incisolobata, Lilium amabile Palib., Siberian iris, Dictamnus dasycarpus Turcz., Atractylodes ovata(Thunb.) DC., and Lysimachia clethroides Duby.
Effects of Coptidis Rhizoma on Lowering Lipid and Oxidative Stress
Lee, Eun ; Cho, Eun-Joo ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 544~547
The effects of hwangryun(Coptidis Rhizoma) on lowering lipid and oxidative stress in the induced obesity rat was observed. The concentration of plasma triglyceride in hwangryun treatment groups showed the low values compared to the control group, and as the increased hwangryun, the concentration of triglyceride decreased. The concentration of plasma total cholesterol decreased in all hwangryun treatment groups. However the concentration of plasma HDL-cholesterol and LDL-cholesterol showed no significantly difference in all the treatment groups. Thiobarbituric acid reactive substance(TBARS) concentration in plasma and liver showed low values in all hwangryun treatment groups compared to the control group. Glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) activities showed no significantly difference in all the treatment groups. However catalase(CAT) activity showed a tendence to increase in hwangryun groups, and in 200mg/kg hwangryun treatment group showed significantly a high value than the control group. Summarizing the results above, hwangryun has the functional materials that lowering lipid and works with oxidative stress.
Anti-inflammatory Effect of Scutellariae Radix
Lee, Eun ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 548~552
This research is the basic research to develop new anti-inflammatory medicine by feeding Scutellariae Radix extract to lipopolysaccharide(LPS) exposed rats, and analyzed it's effect on inflammatory response by LPS derivation. As a result, Plasma interleukin-
and Plasma interleukin-6(IL-6) concentration showed the highest point at 5h after LPS injection, and in this time, the concentration of
and IL-6 in the Scutellariae Radix extract groups at 200mg/kg and 300mg/kg showed lower values than that of control group. Plasma tumor necrosis
concentration after LPS injection showed the highest point at 2h and showed similar level till at 5h.
concentration at 2h after LPS injection showed the low value only in the Scutellariae Radix extract 300mg/kg group compared to others, and in 5h, the all Scutellariae Radix extract groups showed lower value than that of the control group. Plasma interleukin-10(IL-10) concentration increased at 2h after LPS injection and reached the highest at 5h. After LPS injection the IL-10 concentration at 2h, the Scutellariae Radix extract injection group at 300mg/kg showed higher value than that of the others, and in 5h after LPS injection, Scutellariae Radix extract 200mg and 300mg groups showed higher value than the control group. Concluding from the above results, in inflammatory response by LPS derivation, the Scutellariae Radix gives positive effect.
Alpha-Tocopherol Contents of Peanut Seeds Depend on Varieties and Processing Methods
Doo, Hong-Soo ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 553~557
The purpose of this study is to establish an extraction and analysis method for
-tocopherol, and to then distinguish among varieties. The
-tocopherol contents of 22 varieties of peanut seeds were analyzed by HPLC. Peanut seeds of cv. Palkwang were processed in two ways, by boiling and roasting. The
-tocopherol contents of the two types of peanuts were compared with fresh seeds without any processing.
-Tocopherol was detected at a retention time of 2.95 minutes. Five standards of
-tocopherol covering a range of
were made up in 2% isopropyl alcohol/n-hexane and analyzed in duplicate. The
-tocopherol contents differed according to extraction temperature. The contents were
or less at
, but they were decreased at
or higher. The
-tocopherol contents in 22 peanut varieties were
according to variety. Fresh peanuts contained
-tocopherol, while boiled peanuts contained
-tocopherol, and roasted peanuts contained
Trans-Resveratrol Contents of Peanut Seeds Depend on Varieties and Processing Methods
Doo, Hong-Soo ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 558~562
The high-performance liquid chromatography(HPLC) method for the determination of trans-resveratrol in 34 germplasms and processing methods of peanut seeds has been modified. Peanut germplasms contained trans-resveratrol contents of
, but findings for the testa color were not significant. However, two germplasms, 'KIGAN' and 'CS1', contained more trans-resveratrol contents than the other germplasms. The contents of their were
. The tested processing methods caused no significant changes in trans-resveratrol contents. The contents of fresh, boiled, and roasted peanuts were 0.36, 0.32, and
, respectively in cv. Palkwang, and 0.22, 0.22, and
, respectively, in cv. Jakwang. Differences were not significant among fresh, boiled, and roasted peanuts. The grains of 'Palkwang' and 'Jakwang' contained trans-resveratrol contents of
, and testa contained
, respectively. However, when comparing absolute quantity, the trans-resveratrol contents appears to be approximately
times higher in the testa than in the grain of the peanut, although the total contents were not different because the ratio of testa was low in peanut seeds.
Analysis of Genetic Relationship Among Native Pears Grown in Korea and Several Commercially Developed Cultivars from Two Pyrus Species Based on RAPD Analysis
Cho, Dong-Wook ; Oh, Jin-Pyo ; Chung, Kyu-Hwan ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 563~569
RAPD analysis showed that all the OTUs of 'Sandolbae' were the same species because amplified band patterns of all samples generated by each of 5 random primers were identical. Even though there were different environmental conditions, all the "Chuiangne" trees from three different places were the same species, and also all the "Cheongshilli" trees were the same species too. No genetic variations were detected between native Korean pears grown in the habitats and in the research field. Because 212 polymorphic bands were generated by 9 primers selected through primer screening, they were possible to analyze genetic relationship among naturally growing three native Korean pears and nine cultivars of Pyrus pyrifolia and P. communis. Based on the RAPD analysis, three main groups were formed. The first group represented the Six P. pyrifoia cultivars, the second group was the three native Korean pears, and the last group was the three P. communis cultivars. Genetic distance between 'Wonwhang' and 'Chojuro' was closer than other cultivars in group 1 since dissimilarity index value between these two cultivars was 50.82. However, genetic distance between 'Niitaka' and 'Chojuro' was the most distant compared to the others in group 1. In group 2, 'Sandlobae' was genetically closer to 'Chuiangne' than 'Cheongshilli' because dissimilarity index value between 'Sandlobae' and 'Chuiangne' was smaller, 50.82, than the value between 'Sandlobae' and 'Cheongshilli', 63.636. In group 3, 'Old Home' was genetically closer to 'Bartlett' than 'Kaiser Alexander(or Bosc)'. Group 3 composed of P. communis cultivars was genetically further than other two groups, P. pyrifolia cultivars and native Korean pears.
Ecological Characteristics of Sorbus commixta Hedl. Natural Populations in Mt. Chiri
Kim, Sea-Hyun ; Han, Jin-Gyu ; Chung, Dong-Jun ;
Korean Journal of Plant Resources, volume 20, issue 6, 2007, Pages 570~578
In order to investigate the basic vegetation information for the efficient management of the Sorbus commixta habitat in Korea, 31 plots in Mt. Chiri area were selected of which vegetation types were classified in phytosociological method and ecological characteristics were identified. As a result, the habitat was classified as S. commixta community group which was then subdivided to Prunus padus group and Picea jezoensis group. Prunus padus group was classified in Ainsliaea acerifolia subgroup and Magnolia sieboldii subgroup, while the Picea jezoensis group was classified in Rhododendron mucronulatum subgroup and Echinopanax horridum subgroup. Thus, the forest vegetation of S. commixta was classified in 1 community, 2 groups and 4 subgroups, and found to have 4 vegetation units in total.