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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 22, Issue 6 - Dec 2009
Volume 22, Issue 5 - Oct 2009
Volume 22, Issue 4 - Aug 2009
Volume 22, Issue 3 - Jun 2009
Volume 22, Issue 2 - Apr 2009
Volume 22, Issue 1 - Feb 2009
Selecting the target year
In vitro culture of adventitious root from Rhodiola sachalinensis
Bae, Kee-Hwa ; Yoon, Eui-Soo ; Choi, Yong-Eui ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 281~286
Rhodiola sachalinensis is one of the most popular oriental medicines in East Asia. It is a perennial herb, belonging to the family Crassulaceae, which is mainly distributed in mountains at the altitudes of 1700-2500 m in Baek-Du mountain. Cultivation of this species for the production of medicine materials is not easy in nature, because of restrict habitats. In vitro production of roots can be applicable for the production of medicinal materials. Here, we investigated the optimal conditions for induction and proliferation of adventitious roots in in vitro culture system. Leaf, stem and root segments from R. sachalinensis were cultured on Murashige and Skoog(MS) medium supplemented with the various concentrations of IBA(Indole-3-butyric acid)(0.5, 1.0, 3.0, and 5.0 mg/L) and sucrose(10, 30, 50, and 100 g/L). The optimal explant for adventitious root induction was leaf segment. Induction of adventitious roots was highest on MS medium supplemented with 5.0 mg/L IBA and sucrose 30 g/L. In liquid culture, fresh weight of adventitious roots was highest(15.65 g) on 1/3 strength MS liquid medium supplemented with 5.0 mg/L IBA with 30 g/L sucrose. These results revealed the first attempt for the production of adventitious roots in R. sachalinensis.
Anatomical Characteristics of Korean Mistletoe [Viscum album var. coloratum(Kom.) Ohwi] Stem
Lee, Bo-Duk ; Park, Beyung-Su ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 287~292
Recently, the consumption of mistletoe[Viscum album var. coloratum(Kom.) Ohwi] is increasing because of its good medical effectiveness with the increased concern on the natural medicines and foods. The result obtained from the investigation on the stem tissues of the mistletoe and the oriental chestnut oak, a host plant species, are as follows. Haustorium from the seeds of the mistletoe after their sticking to the branches of the host plant penetrates into the bark where it forms the endophyte system through the active cell division. The endophyte grown in the cambium of the host plant makes the stems and leaves as the outer tissues in a certain time. Even through lignification of the host wood in the branches the oriental chestnut oak was not progressive, its tylosis coas developed partially assembly due to the formation of the endophyte. The stems of the mistletoe consisted of vascular tracheid, selereid, and ray and axial parenchyma, classified as a hardwood without vessels. The vascular tracheids seemed to take a role instead of the vessels in the mistletoe plant from the result that the pits of the vessels in the host branches are linked to the vessel-form tracheid in the mistletoe stems. The constituent ratio of the sclereid cells in the mistletoe stems increased with aging. Furthermore their ratio of the parenchyma cells was higher, which contained the more cell content, compared with the cells of the general woody plant species.
Densified Pellet Fuel Using Woody Core of Industrial Hemp (Cannabis sativa L.) as an Agricultural waste
Han, Gyu-Seong ; Lee, Soo-Min ; Shin, Soo-Jeong ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 293~298
We prepared densified wood pellet by agricultural waste. The hemp woody core was used as replacing wood resource. Hemp was separated into the bast fiber and the woody core by hot steaming treatment. The hemp woody core had a similar lignin content(19.4%) and carbohydrate composition with hardwood(20-25% lignin in hardwood), respectively. Also, the hemp had a low ash content(0.5%), which resulted in a low ash formation in pellet burning. Heating value of the hemp pellet(18.40 MJ/kg) had a very similar to the pellet made by hardwoods. The hemp woody core could be replaced the hardwood for densified wood pellet.
Effect of Ridge Height on Growth and Tuber Yield in Cynanchum auriculatum Royle ex Wight
Nam, Sang-Young ; Kim, In-Jae ; Kim, Min-Ja ; Rho, Chang-Woo ; Lee, Jung-Gwan ; Yun, Tae ; Min, Kyeong-Beom ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 299~303
This study was conducted to increase the productivity and quality of C. auriculatum Royle ex Wight according to the various ridge height. The higher ridge height increased the vine length, leaf length, leaf width, leaf number, chlorophyll content, and leaf dry weight; however, the lower ridge height increased the stem diameter and branch numbers. The tuber number and length was increased at less than 20cm of ridge height, but the overall growth was retarded in the treatment of over 20cm ridge height. The tuber diameter was also thicker in the lower ridge. The rootlet ratio among the non-commercial tuber was increased in the 20cm and 30 cm ridge, and the decayed tuber ratio was increased in the lower ridge. The yield of tuber has increased with 3% and 11% in the 20cm and 30cm ridge height compared to 10cm ridge(515kg/10a).
Development of Optimal Cultivation Conditions and Analysis of Antioxidant Activities of Arctium lappa Sprout Vegetables
Lee, Moo-Yeul ; Shin, So-Lim ; Park, Seon-Hee ; Kim, Na-Rae ; Chang, Young-Deug ; Lee, Cheol-Hee ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 304~311
This study was conducted to develop functional sprout vegetables with antioxidant effects using seeds of Arctium lappa. The seeds germinated vigorously under light at
, reaching germination rate of 82% within 4 days. Germinated seeds were placed under darkness at various temperatures to force growth in length, and it was demonatrated that
was optimum temperature. Greening treatment reduced growth in length, but promoted growth of cotyledons. Harvested A. lappa sprout vegetables maintained freshness longer at
, rather than
. Ventilation holes in storage containers had no effects on storage periods. Antioxidant activity of vegetable that received greening treatment for 1-3 days was investigated, and it was shown that free radical scavenging effects and ferrous ion chelating effects was higher than those of commercially available brocoli, cauliflower, pea and bean sprout. Contents of total polyphenol and flavonoid were also higher, especially by 3 day greening. The longer the treatment, the more the inhibition on peroxidation of linoleic acid. Sprout vegetable of A. lappa had higher antioxidant activity compared with adult plant. In conclusion, sprout vegetable of A. lappa has great potentiality for use as one of sprout vegetables.
Anti-cancer Activities of Extract from the Bark of Melia azedarach L. var. japonica Makino
Kim, Hyun-Woo ; Kang, Se-Chan ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 312~316
In the present study, the anti-cancer activity of 80% ethanol extracts from 120 kinds of medicinal herbs and native plants were investigated. Among them, the barks of Melia azedarach L. var. japonica Makino showed the highest cytotoxicity in HCT-15 human colon cancer cell. With this result, we carried out hollow fiber (HF) assay and anti-metastasis study to confirm the anti-cancer effects of M. azedarach var. japonica. In MTT assay, M. azedarach var. japonica.inhibited the proliferation of HCT-15 cells in dose-dependent manner. HF assay was carried out using A549 human adenocarcinoma cell, HCT-15 and SK-Hep1 human liver cancer cell via intraperitoneal (IP) and subcutaneous (SC) site. As a results, SK-Hep1 implanted in IP site showed the highest cytotoxicity. The result from metastatic model using B16/BL6 mouse corresponded to that of HF assay. These results suggest that the ethanol extract from M. azedarach var. japonica. might have a potent anti-cancer activity and advanced study is needed for the development of novel natural anti-cancer drug.
Melanogenesis Inhibition Effect of Rosa multiflora Extracts in B16 Melanoma Cells
Ha, Se-Eun ; Kim, Hyoung-Do ; Park, Jong-Kun ; Chung, Yeon-Ok ; Kim, Hyun-Jong ; Park, Nou-Bog ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 317~322
In the present study, the effect of ethanol extracts of leaf and root of Rosa multiflora on the proliferation of B16 cells, tyrosinase activity and melanin synthesis were investigated. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay demonstrated that the cell viability upon treatment with Rosa multiflora extract(0-200
/ml) was similar to that of untreated control. Treatment with leaf or root extracts(200
/ml) decreased the in vitro tyrosinase activity to about 65% of that in untreated control. Similarly, the intracellular tyrosinase activity of B16 cells decreased in a concentration-dependent manner. Furthermore, the melanin synthesis of B16 cells decreased by the two extracts in a concentration dependent manner. However, the extracts did not change the level of tyrosinase mRNA, as determined by RT-PCR. These results demonstrated that the Rosa multiflora extracts inhibit the tyrosinase dependent melanin biosynthesis, and therefore, are candidates for skin-whitening agents.
Antioxidant Activity and Functional Components of Corn Silk(Zea mays L.)
Ku, Kang-Mo ; Kim, Soon-Kwon ; Kang, Young-Hwa ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 323~329
In order to find out superior corn line which has a strong antioxidant activity in the corn silk, antioxidant compounds and antioxidant activity were measured from various inbred and F1 lines. As a result, the contents of phenolic and flavonoid compounds range from 532 mg/100 g to 3,274 mg/100 g and from 980 mg/100 g to 2,420 mg/100 g respectively. Absorbance at 517 nm for contents of anthocyanins ranges from 0.05 to 0.76. Correlation coefficients between antioxidant compounds and various antioxidant assays such as DPPH, ABTS and FRAP were analyzed. Correlation coefficients between antioxidant activity and phenolic compounds and anthocyanin were significantly high in ABTS and FRAP assays. Considering acidic extraction condition and intervention between anthocyanins and DPPH solution, ABTS assay and FRAP assay are more suitable methods to evaluate antioxidant activity of corn silks. Especially, ABTS assay is thought to be the best method among three assays because the antioxidant activity in ABTS assay showed high correlation with phenol, flavonoid and anthocyanin compounds respectively. Among the samples, S15 which showed the highest contents of total phenolic compounds and the most potent antioxidant activity in ABTS and FRAP assay will be a good source for functional material.
Effects of Short-Term Ingestion of Wood Vinegar on Antioxidative Defense System in Trained Rats
Lee, Soo-Chun ; Kim, Dong-Ki ; Kim, Jae-Woo ; Seo, Hyo-Bin ; Lee, Yoon-Kyung ; Jeon, Byung-Duk ; Lee, Dong-Woon ; Ryu, Sung-Pil ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 330~336
The purpose of this study is to know the effect of wood vinegar ingestion on antioxidative defense system in trained rats. Twenty-eight male Sprague-Dawley male rats were used as the subject. Experimental groups were divided into CON-SED(control + sedentary), CON-EX(control + exercise), WV-SED(wood vinegar ingestion + sedentary), and WV-EX(wood vinegar ingestion + exercise), respectively. Body weight and stored fat weight(mesentery, retroperitoneal and epididymal) were not significantly differed in each group. Concentration of TG and FFA was not differed by wood vinegar ingestion. Concentration of glucose was revealed significant difference in exercise group rather than sedentary group, but there was no difference by wood vinegar ingestion between the groups. Contents of SOD in WV-EX group was significantly higher than other three groups. Contents of MDA in CON-EX group was significantly lower than CON-SED group, while the difference between CON group and WV group was not observed. In conclusion, wood vinegar ingestion with exercise seems to have a positive beneficial effects on reducing oxidative stress.
Effect of Medium Components and Culture Methods on Prothallus Propagation of Pteridium aquilinum var. latiusculum (Desv.) Underw. ex Hell.
Shin, So-Lim ; Lee, Moo-Yeul ; Choi, Jae-Sun ; Lee, Cheol-Hee ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 337~342
Present studies were conducted to evaluate the effects of medium strength(MS and Hyponex), carbon sources and their concentrations, agar concentrations, and inoculation amounts on prothallus propagation of Pterdium aquilinum var. latiusculum(Desv.) Underw. ex Hell in vitro. The optimum MS medium strength for prothallus propagation was 2MS concentration. Phosphate source was most effective for prothallus growth of P. aquilinum var. latisculum. The addition of 1% sucrose or glucose to MS medium promoted prothallus multiplication. Growth of prothallus was not affected by agar concentration. Propagation of homogenized prothallus was vigorous even in liquid medium. Chopped gametophytes(100 and 200 mg) were inoculated on 250 ml
flask with 100 mL of 2MS concentration medium and suspension culture was done at 100 rpm for 22 days. After 20 days, prothallus multiplication slowed down, so 100 mg of chopped prothalli is recommended for initial inoculation, since initial amount of inoculum did not affect subsequent prothallus multiplication. Consequently after 20 days of suspension culture, prothallus should be subcultured or transplanted outside of growing vessels.
Polyphenol and Anti-oxidant Effects of Kalopanax septemlobus Koidz. Leaf Extracts
Jun, Dong-Ha ; Lee, Jin-Tae ; Cheon, Soon-Ju ; Lee, Chang-Eon ; Kim, Tae-Hoon ; Lee, Do-Hyung ; Han, Jin-Gyu ; Kim, Sea-Hyun ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 343~348
The aim of the study was to assess the cosmeceutical activity of Kalopanax septemlobus leaf and it is possible that can be used as a cosmetic ingredient for applicaiton of cosmetic industries. The concentration of total phenolic compound of hot water and 70% EtOH extracts of K. septemlobus leaf showed 104 mg/L and 125 mg/L respectively. In the result of DPPH(1,1- diphenyl-2-picryl -hydrazyl) scavenging radical activity, 70% EtOH extracts of K. septemlobus leaf showed 93.1% and it was similar to BHA(butylated hydroxyanisole) effect at 1,000ppm concentration. Xanthine oxidase inhibition of hot water extracts and 70% EtOH extracts of K. septemlobus leaf were 46.6% and 60.4% at 1,000ppm, respectively. In these results, K. septemlobus leaf has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.
Changes of Antioxidant Effects According to Greening Period of Astragalus membranaceus var. membranaceus, Senna occidentalis, Dianthus longicalyx, and Plantago asiatica Sprout Vegetables
Lee, Cheol-Hee ; Shin, So-Lim ; Kim, Na-Rae ; Yoon, Sung-Eun ; Kim, Su-In ; Baek, Seol-Hee ; Hwang, Ju-Kwang ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 349~358
The potential use of 4 plant species, Astragalus membranaceus var. membranaceus, Senna occidentalis, Dianthus longicalyx and Plantago asiatica, as new sprout vegetables with high antioxidant function was examined in the present experiments. Seeds of above plants were allowed to germinate under light condition, and seedlings were maintained under dark condition for shoot growth in length for contain period of time. Then the seedlings were put under light for photosynthesis (greening treatment) for the period of 0
3 days. Samples were collected to analyze the changes in antioxidant levels and activity, and it was observed that antioxidant substances were affected by greening treatments, depending on plant species. In A. membranaceus, the contents of total polyphenol was highest with no greening, total flavonoids with 3 days greening, DPPH radical scavenging effects with no greening, ABTS scavenging with 1 day greening,
chelating effects with no greening, and inhibitory activity against linoleic acid peroxidation with 3 day greening. In S. occidentalis, highest levels of antioxidant activity and radical scavenging effects were obtained by 2 day greening,
chelating effects by no greening and inhibitory activity against linoleic acid peroxidation by 1 day greening. In D. longicalyx, highest levels of antioxidant activity and
chelating effects were obtained by 2 day greening,
chelating effects by no greening and inhibitory activity against linoleic acid peroxidation by 1 day greening. In D. longicalyx, highest levels antioxidant activity and
chelating effects were observed with 3 day greening, and highest radical scavenging effects and inhibitory activity against linoleic acid peroxidation with no greening treatment. In P. asiatica, antioxidant activity and radicals scavenging effects were highest with 2 day greening, whereas highest chelating effects was obtained with no greening and highset inhibitory activity against linoleic acid peroxidation with 3 day greening. As the length of greening treatments influenced the antioxidant levels and function in plant species tested in this experiments, different culture methods are recommended for different plant species to get maximum health benefits out of sprout vegetables.
Labor-saving practices in Tartary buckwheat(Fagopyrum tataricum) production
Lim, Yong-Sup ; Park, Byoung-Jae ; Park, Cheol-Ho ; Park, Jong-In ; Kim, Yang-Sik ; Park, Kwang-Ho ; Kang, Yun-Kyu ; Chang, Kwang-Jin ;
Korean Journal of Plant Resources, volume 22, issue 4, 2009, Pages 359~363
In order to establish labor-saving culture technology in Tartary buckwheat, three cultural practices: hand planting, drill sowing machine and soil cover direct seeding machine, were compared.The highest grain yield was found in soil cover direct seeding plot with a value of 3.4 g per plant. As a result, grain yield may be estimated to be 113kg in soil cover direct seeding and 80kg in hand scattering. In addition, for the weed control, three herbicide treatments: single use of Alachlor, mixture and combination of Alachlor and Paraquat dichloride were conducted. the mixture showed over 90% weed control value, and the highest grain yield was found in the combination treatment. Combine machine was effective to reduce the ratio of grain loss and working hour by enhancing the working efficiency to 15