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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Plant Resources
Journal Basic Information
Journal DOI :
The Plant Resources Society of Korea
Editor in Chief :
Volume & Issues
Volume 27, Issue 6 - Dec 2014
Volume 27, Issue 5 - Oct 2014
Volume 27, Issue 4 - Aug 2014
Volume 27, Issue 3 - Jun 2014
Volume 27, Issue 2 - Apr 2014
Volume 27, Issue 1 - Feb 2014
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Anti-osteoporotic Effects of Unripe Fructus of Rubus coreanus Miquel in Osteoblastic and Osteoclastic Cells
Kim, Hyo Jin ; Sim, Dong-Soo ; Sohn, Eun-Hwa ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 593~600
DOI : 10.7732/kjpr.2014.27.6.593
Osteoporosis is a progressive bone disease characterized by low bone mass which is caused by disturbance in the balance between the activities of osteoblasts and osteoclasts. Postmenopausal osteoporosis is one of the most common disorders in women after menopause, which is linked to an estrogen deficiency and characterized by an excessive loss of trabecular bone. Rubus coreanus has been used for their various pharmacological properties in Asia as a traditional medicine. To investigate the effect of unripe fruits of R. coreanus 30% ethanol extract (RCE) on osteoblast-like cells (MG63) differentiation, we examined the effects of RCE on in vitro osteoblastic differentiation markers, alkaline phosphatase (ALP) activity and receptor activator of nuclear factor
-B ligand (RANKL) and osteoprotegerin (OPG) expression. The high concentration (50 and
) of RCE markedly increased ALP activity, whereas decreased the RANKL/OPG. We also investigated the effect of RCE on M-CSF plus RANKL-induced differentiation of pre-osteoclast cells (RAW 264.7). RCE treatment remarkably inhibited M-CSF/RANKL-induced formation of osteoclast-like multinuclear cells from RAW 264.7 cells. Moreover, the inhibitory effect of RCE was reduced by selective estrogen receptor-
antagonist. Our research suggests that suggested that unripe fruits of R. coreanus may act beneficial effects on bone mass by regulating both osteoblast and osteoclast.
Screening of Plants with Inhibitory Activity on Cellular Senescence
Lee, Seung-Eun ; Kim, Jae-Ryong ; Noh, Hyung-Jun ; Kim, Geum-Sook ; Lee, Jeong-Hoon ; Choi, Jehun ; Lee, Dae-Young ; Kim, Seung-Yu ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 601~609
DOI : 10.7732/kjpr.2014.27.6.601
In this study, the effect of plant extract on the senescence action and cell survival rate in two types of cells, in which aging was derived by adriamycin, was analyzed to find the materials for suppressing cell senescence from natural resources. The results are as follows. For human umbilical vein endothelial cells (HUVECs), the fruit of Physalis angulata L. and the aerial part of Synurus deltoides (Aiton) Nakai showed excellent cell-senescence inhibition activities in a treatment concentration-dependent manner, demonstrating the high possibility for utilization as a material for prevention and treatment for vascular diseases. The water extract from the root of Polygonatum odoratum var. pluriflorum for variegatum Y. N. Lee showed potent cell-senescence inhibitory effect for human dermal fibroblasts (HDFs). Thus it is considered that the additional study on the plant needs for elucidating the possible utilization as material for skin health improvement.
Analysis of the Phenolic Content and Antioxidant Activities of Soybean Extracts from Different Regions and Cultivars
Nam, Jung-Hwan ; Kang, Suk-Woo ; Hong, Su-Young ; Kim, Su-Jeong ; Jin, Young-Ik ; Kim, Hyun-Sam ; Yoon, Young-Ho ; Jeong, Jin-Cheol ; Pan, Cheol-Ho ; Um, Byung-Hun ; Nho, Chu-Won ; Ok, Hyun-Choong ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 610~621
DOI : 10.7732/kjpr.2014.27.6.610
Phenolic compounds such as isoflavones contribute to the antioxidant properties of soybean. This study investigated differences in the phenolic content and antioxidant activity of ten soybean cultivars grown in two different locations in Korea. We observed a wide range of variation in total isoflavone and phenolic content by location and cultivar. The total isoflavone content of cultivars grown at Pyeongchang (sub-highland) was significantly higher than that in cultivars grown at Gangneung (lowland). In contrast, the total phenolic content of cultivars grown at Gangneung was greater than that of cultivars grown at Pyeongchang. The radical scavenging activity of DPPH was similar to that of the total phenolic content rather than that of the total isoflavone content. These results suggest that antioxidant activity of soybean was associated with phenolic compounds rather than isoflavones. To identify the individual antioxidant components, we used an on-line HPLC-
-based assay system, ESI/MS, and NMR. The results showed that the strongest antioxidant activity was linked to epicatechin.
Hypoglycemic Activity of the Hexane Extract of Oriental Melon (Cucumis melo L. var. makuwa Makino) Seeds and Its Active Compounds
Chen, Lei ; Kim, Hye Suk ; Choi, Bo-Ram ; Yang, Shaonan ; Xu, Enning ; Suh, Jun Kyu ; Kang, Young-Hwa ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 622~628
DOI : 10.7732/kjpr.2014.27.6.622
The aim of this work is to evaluate the potential of oriental melon (Cucumis melo L. var. makuwa Makino) seeds for the management of type 2 diabetes by controlling glucose absorption. The
-amylase inhibitory effects of the hexane extracts from oriental melon seeds were investigated. A bioassay-guided fractionation technique was used to elucidate the principal active components. The results show that the hexane extract from oriental melon seeds exhibited high inhibitory activities against
-amylase. The hexane extract was further fractionated into four sub-fractions. Among them, the sub-fraction F-1 exhibited the most potent anti-diabetic effect. The active components were isolated and identified by gas chromatography/mass spectroscopy (GC-MS). Free fatty acids showed significant hypoglycemic activity (p < 0.001) and fatty acid composition influenced enzyme inhibitory activities. These results suggest that oriental melon seeds could be used to prevent type 2 diabetes.
Evaluation of Chlorophyll Content, Antioxidant Activity and Phenolic Compounds in the Seedlings of Rice-type Tartary Buckwheat
Sharma, Pankaja ; Lee, Kooyeon ; Park, Cheol Ho ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 629~634
DOI : 10.7732/kjpr.2014.27.6.629
Rice type tartary buckwheat is used as a substitute for rice in many Asian countries due to its easy dehulling character. The objective of the present study was to determine the chlorophyll, total polyphenol (TP), total flavonoid (TF), antioxidant activity and to quantify the bioactive compounds rutin, quercetin and chlorogenic acid in the seedlings of rice-type tartary buckwheat (RTTB). Young seedlings exhibited higher antioxidant (DPPH radical inhibition) activity in dose dependent manner. TP and TF content were highest (
TAE/100 mg dw and
QE/100 mg dw respectively) in 3 days after germination (DAG) seedlings compare to 6 and 9 DAG. The contents of rutin and quercetin increased with growing stage of seedlings. However, the chlorogenic acid decreased with increasing growth. Overall, RTTB seedlings can be regarded as a strong source of phenolics and have high possibility for food and nutraceutical application due to their efficient antioxidant properties, higher chlorophyll and phytochemical content.
The Effective Preparation of Flavonoids from Scutellaria baicalensis G
by Diaion HP-20 Resin
Yu, Young-Beob ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 635~641
DOI : 10.7732/kjpr.2014.27.6.635
(Scutellariae Radix) has been used to clear heat and to dry dampness in the stomach or intestines, which manifests as diarrhea or dysenteric disorder. In this study, we investigated the effective preparation of active components in Scutellariae Radix using the methods of solvent extraction and absorption fractionation for the development of new functional food or pharmaceuticals. The marker substances, baicalin, baicalein, wogonoside, and wogonin were directly isolated from the Scutellariae Radix. There chemical structures were elucidated by spectroscopic analysis. The Scutellariae Radix was extracted with hot water. To enhance yield of flavonoids in Scutellariae Radix, the hot water extract was dissolved in ethanol with concentration dependent manner. The precipitates were separated using centrifugal techniques at 10,000 rpm. Supernatant liquid was applied to the HPLC for quantification of major compounds. Separately, the hot water extract was absorbed on Diaion HP-20 resin. And then, the absorbed fraction was eluted with methanol for HPLC. The contents of baicalin, baicalein, wogonoside and wogonin in different treatment methods were analyzed by HPLC. Total amount of four major components were 16.9% in 50% ethanol extract, 21.7% in 70% ethanol extract, 20.5% in 90% ethanol extract, and 39.3% in absorbed fraction of Diaion HP-20 resin. In these results, we found that resin absorption method is suitable for the extraction of enriched flavonoids from Scutellariae Radix.
Antioxidant Activities of Agrimonia pilosa Ledeb: In Vitro Comparative Activities of Its Different Fractions
Chen, Lei ; Kang, Young-Hwa ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 642~649
DOI : 10.7732/kjpr.2014.27.6.642
The antioxidant potentials of the methanol extract and its various fractions from Agrimonia pilosa Ledeb were measured as 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging abilities as well as the reducing power decreasing NO in vitro. The methanol extract of Agrimonia pilosa Ledeb showed significantly strong scavenging effects on free radicals of DPPH, ABTS, and NO. Hexane fraction (HF), ethyl acetate fraction (EF) and n-butyl alcohol fraction (BF) were prepared by solvent fractionation. By comparison of polyphenol contents among BF and HF, EF with high polyphenol contents showed the highest DPPH, ABTS, and NO scavenging effects with the value of 50.2%, 80.4%, and 65.7%, respectively, at the highest tested dose. HPLC analysis revealed the presence of various phenolic compounds in EF of Agrimonia pilosa Ledeb. These results suggest that EF could be considered as natural antioxidant sources and dietary nutritional supplements to prevent oxidation-related diseases.
Identification and Quantification of Flavonoids in Korean Wild Grape (Meoru grape, Vitis coignetiae) and Its Pomace
Shim, Kwan-Seob ; Kang, Da-Rae ; Park, Seong-Bok ; Park, Jong Hyuk ; Chung, Yi Hyung ; Kang, Young-Hee ; Shin, Daekeun ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 650~659
DOI : 10.7732/kjpr.2014.27.6.650
Large quantities of Korean wild grape (Vitis coignetiae, KWG) pomace, a by-product of grape juice and wine manufacture, are generated annually, and disposal cost of KWG pomace is now increasing. Therefore, this study was conducted to determine total polyphenol, flavonoid, and anthocyanin content in whole KWG, KWG skin and KWG pomace and to identify and quantify flavonoids found in whole KWG and KWG pomace using LC/MS/MS. Spectral analysis showed high total polyphenol and flavonoid in KWG skin extracted with 75% ethanol (p < 0.05). KWG pomace had higher amount of total polyphenol, flavonoid and anthocyanin than whole grape (p < 0.05). Sixteen flavonoids were identified, but only 10 flavonoids were quantifiable from whole KWG and KWG pomace. Both epicatechin and rutin were the major flavonoids, and 521 or 147 ng/g of epicatechin (p < 0.05) and 305 or 110 ng/g of rutin (p > 0.05) were found in whole KWG and KWG pomace, respectively. The results show that KWG pomace is a very rich source of flavonoids, thus KWG pomace can be used as a functional food additive. Plans to include KWG pomace in food production are necessary.
Genetic Diversity and Population Genetic Structure of Cephalotaxus koreana in South Korea
Hong, Kyung Nak ; Kim, Young Mi ; Park, Yu Jin ; Lee, Jei Wan ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 660~670
DOI : 10.7732/kjpr.2014.27.6.660
The Korean plum yew (Cephalotaxus koreana Nakai) is a shade-tolerant, coniferous shrub. The seeds have been used as a folk medicine in Korea, and an alkaloid extract (HTT) is known to have anticancer properties. We estimated the genetic diversity of 429 trees in 16 populations in South Korea using 194 polymorphic amplicons from seven combinations of AFLP primer-restriction enzymes. The average number of effective alleles and the percentage of polymorphic loci were 1.37 and 79.4%, respectively. Shannon's diversity index and the expected heterozygosity were 0.344 and 0.244, respectively. We divided 16 populations into four groups on the UPGMA dendrogram and the PCA biplot. The first two principal components explained 84% of the total genetic variation. Genetic differentiation between populations explained 14% of total genetic variation, and the remaining 86% came from difference between individuals within populations, as determined by an analysis of molecular variance (AMOVA). However, the genetic differentiation did not correlate with the geographic distance between populations from the Mantel test. The Bayesian statistics, which are comparable to Wright's
, respectively. The population genetic diversity was slightly lower, and the strength of genetic differentiation was much weaker, than the average of those plants having similar life histories, as assessed using arbitrary marker systems. We discuss strategies for the genetic conservation of the plum yew in Korea.
Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences
Son, OGyeong ; Yoon, Chang Young ; Park, SeonJoo ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 671~679
DOI : 10.7732/kjpr.2014.27.6.671
Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.
Molecular Authentication of Acanthopanacis Cortex by Multiplex-PCR Analysis Tools
Kim, Min-Kyeoung ; Jang, Gyu-Hwan ; Yang, Deok-Chun ; Lee, Sanghun ; Lee, Hee-Nyeong ; Jin, Chi-Gyu ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 680~686
DOI : 10.7732/kjpr.2014.27.6.680
Acanthopanacis Cortex has been used for oriental medicinal purposes in Asian countries especially in Korea and China. In the Korean Pharmacopeia, the cortexes of the dried roots, stems and branches of all species in Eleutherococcus and Eleutherococcus sessiliflorus are known as 'Ogapi'. Mostly the cortexes of E. gracilistylus roots and E.senticosus roots were used as 'Ogapi' in China and Japan, respectively. Therefore, the purpose of this study was to determine and compare the molecular authentication of Korean 'Ogapi' by using the ribosomal internal transcribed spacer (ITS) region. The ITS region has the highest possibility of effective and successful identification for the widest variety of molecular authentication. The ITS region was targeted for molecular analysis with Single nucleotide polymorphisms (SNPs) specific for morphologically similar to E. gracilistylus, E. senticosus, E. sessiliflorus from their adulterant, moreover, E. sieboldianus were detected within sequence data. Thus, based on these SNP sites, specific primers were designed and multiplex PCR analysis were conducted for molecular authentication of four plants (E. gracilistylus, E. senticosus, E. sessiliflorus, and E. sieboldianus). The findings of results indicated that ITS region might be established multiplex-PCR analysis systems and hence were proved to be an effective tools for molecular evaluation and comparison of 'Ogapi' with other plants.
Blast Resistant Genes Distribution and Resistance Reaction to Blast in Korean Landraces of Rice (Oryza sativa L.)
Song, Jae Young ; Lee, Gi-An ; Choi, Yu-Mi ; Lee, Sukyeung ; Lee, Kwang Beom ; Bae, Chang-Hyu ; Jung, Yeonju ; Hyun, Do-Yoon ; Park, Hong-Jae ; Lee, Myung-Chul ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 687~700
DOI : 10.7732/kjpr.2014.27.6.687
Rice blast (Magnaporthe oryza B.) is one of the most important diseases in rice that causing great yield losses every year around the world. It is important to screen valuable genetic resources for improving blast resistance. This study was conducted to identify the blast resistance in 279 Korean rice landraces using blast nursery tests and isolate inoculum screening. The results showed that 11 landrace accessions found to be resistant to rice blast in blast nursery and inoculation screening tests and the degree of lesions in most accessions showed that they were susceptible to reactions. In order to find the distribution of blast resistant genes, a molecular survey was conducted to identify the presence of major blast resistance (R) gene in 279 Korean landraces. The results revealed that their frequency distribution was Pik-m (36.2%), Piz (25.4%), Pit (13.6%), and Pik (10%). Besides, the frequency distribution of Piz-t, Pii, Pik-m/Pik-p, Pi-39(t), Pib, Pi-d(t)2, Pita/Pita-2 and Pi-ta genes were identified as less than 10%. The results did not consist with the reactions against blast diseases between genotypes and phenotypic part of the nursery tests and isolate inoculation. For concluding these results, we used genome-wide SSR markers that have closely been located with resistance genes. The PCoA analysis showed that the landrace accessions formed largely two distinct groups according to their degree of blast resistance. By comparing genetic diversities using polymorphic information contents (PIC) value among the resistant, total and susceptible landraces, we found that PIC values decreased in four SSR markers and increased in six markers in the resistant accessions, which showed contrary to total and susceptible groups. These regions might be linked to resistance alleles. In this study, we evaluated the degree of blast resistance and the information about the distribution of rice blast resistant genes in Korean rice landraces. This study might be the basis for association analysis of blast resistance in rice.
Fruit Set and Fruit Characteristics of Highbush Blueberries (Vaccinium corymbosum cvs. Bluecrop, Coville, and Northland) in an Open Field and a Rain Shelter
Kim, Su Jin ; Kim, Jin Gook ; Ryou, Myung Sang ; Park, Kyo-Sun ; Kim, Hong-Lim ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 701~706
DOI : 10.7732/kjpr.2014.27.6.701
Three highbush blueberries (Vaccinium corymbosum cvs. Bluecrop, Coville, and Northland) grown in an open field and in a rain shelter were compared to evaluate the potential benefits of rain-sheltering systems on growth and fruit quality. Shrubs grown in rain shelter flowered 5-14 days earlier and were in full bloom earlier than those in the open field. All three cultivars set fruit by nearly 90% of flowers when they were grown in the open field, but was markedly reduced when they were grown in the rain shelter, to approximately 50% for 'Bluecrop' and 'Coville'. Fruit sets ratio of 'Northland' grown in the in the rain shelter was 14.5. Fruit from greenhouse-grown 'Northland' were larger, heavier, and had more seeds per berry. Soluble sugar content (SSC) of fruits varied both with the cultivars and growing conditions. SSC of fruits grown in the greenhouse was higher in 'Bluecrop' compared to that grown in the field, but this pattern was reversed in 'Northland'. Titratable acid was significantly higher in fruits from rain shelter-grown shrubs of both 'Bluecrop' and 'Northland' than in fruits from open field-grown shrubs (P < 0.05), although there was no difference in TA with respect to growing conditions for 'Coville'. The number of seed per fruit, fruit length and diameter, weight, SSC, and TA were similar in 'Coville' shrubs grown in the open field and the rain shelter. Fruit firmness of 'Bluecrop', 'Coville', and 'Northland' was higher in the rain shelter than in the open field. 'Coville' and 'Northland' fruits harvested in the open field had higher blue chroma than those harvested in the rain shelter. However, this pattern was reversed for 'Bluecrop'. On the whole, sheltering from rain affected most fruit characteristics of the three cultivars differently. Therefore, suitable blueberry cultivars for the rain shelter should be chosen by purpose.
Anti-inflammatory Effects of Allium victorialis Extract in Lipopolysaccharide Exposed Rats and Raw 264.7 Cells
Lee, Eun ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 707~713
DOI : 10.7732/kjpr.2014.27.6.707
This study examined the inflammatory reaction effects of Allium victorialis var. platyphyllum in vivo at the time of a lipopolysaccharide (LPS) shock in rats, and in vitro in cultured Raw 264.7 cells, with the aim of facilitating the development of a new anti-inflammatory medicine. Plasma concentrations of interleukin (IL)-
, IL-6, tumor necrosis factor
), and IL-10 in rats peaked 5 h after LPS treatment in all experimental groups, with those of IL-
, IL-6, and TNF-
being significantly lower in all animals treated with A. victorialis than in the control group at that time point. Conversely, the plasma concentration of IL-10 was higher in the rats treated with 300 mg/kg A. victorialis extract than in the control group at both 2 and 5 h after LPS treatment. Concentrations of IL-
and IL-6 in the liver of rats treated with A. victorialis extract were significantly lower than those of the saline-treated control group. However, the liver concentrations of TNF-
and IL-10 did not vary significantly between the four animal groups. Similarly, concentrations of IL-
, IL-6, and TNF-
obtained from cultured Raw 264.7 macrophages were lower in all of the A.-victorialis-extract-treated groups than in the control group. Although the concentration of IL-10 in the A.-victorialis-extract-treated groups tended to be greater than in the control group, the differences between groups were not statistically significant. Together the findings of this study suggest that A. victorialis var. platyphyllum contains functional substances that are involved in inflammatory reactions.
DNA Damage Protection and Anti-inflammatory Activity of Different Solvent Fractions from Aruncus dioicus var. kamtschaticus
Zhang, Qin ; Kim, Hye-Young ;
Korean Journal of Plant Resources, volume 27, issue 6, 2014, Pages 714~719
DOI : 10.7732/kjpr.2014.27.6.714
This study investigated DNA damage protection and anti-inflammatory activity of different solvent fractions from Aruncus dioicus var. kamtschaticus (A. dioicus) aerial parts water extract. As for DNA damage protection, distilled water (
) fraction displayed the most powerful protection for DNA damage at a concentration of 1 mg/ml. As for anti-inflammatory activity, dichloromethane (
) fraction exhibited the highest NO inhibition activity, ranging from 61% to 19% (
). Furthermore, the levels of pro-inflammatory cytokines mRNA expressions and intracellular reactive oxygen species (ROS) were employed to verify the anti-inflammatory activity of the
fraction on further researches. It could be concluded that A. dioicus had a significantly effect of DNA damage protection and anti-inflammatory activity which also as an essential edible vegetable and medicinal species.