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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 15, Issue 2 - Dec 1973
Volume 15, Issue 1 - Jun 1973
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Effect of the Dietary Composition (quantitative ratio between carbohydrate and protein) and the Rearing Temperature during the 4th Moulting Period on Amylase activity of Digestive Juice in the Silkworm, Bombyx mori L.
Journal of Sericultural and Entomological Science, volume 15, issue 1, 1973, Pages 1~7
his study has been carried out to investigate amylase activity of digestive juice in the 5th day of the 5th instar influenced by the dietary composition (quantitative ratio between carbohydrate and protein) and the rearing temperature during the 4th moulting period. The larvae grew on three kinds of semi-synthetic diets. The A-diet has more carbohydrate than the others, the B-diet has carbohydrate in 1 : 2 with protein, and the C-diet has more protein than the others. All the diets were kept at 16
during the 4th moulting period. Amylase activity of digestive juice at the 5th day of the 5th instar was analyzed by Fuwa's method. The results were as follows. 1. Both A and C-diets were worse than B-diet in the larval growth and development. 2. The dietary composition influencing amylase activity of digestive juice was not related to the rearing temperature during the 4th moulting period. Amylase activity was stronger in C-diet, B-diet and A-diet order. 3. It was found that amylase activity at 32
was stronger than that at 16
in all kinds of diets. 4. There was an inter-action in amylase activity of male larval digestive juice between the dietary composition and the rearing temperature during 4th the moulting period.
Effect of the Dietary Composition (quantitative ratio between carbohydrate and protein) and the Rearing Temperature during the 4th Moulting Period on Proteinase Activity of Digestive Juice and Sucrase Activity of Midgut Tissue in the Silkworm, Bombyx mori L.
Journal of Sericultural and Entomological Science, volume 15, issue 1, 1973, Pages 9~14
This study has been carried out to investigate proteinase activity of digestive juice and sucrase activity of midgust tissue in the 5th day of the 5th instar influenced by the dietary composition (quantitative ratio between carbohydrate and protein) and tile rearing temperature during the 4th moulting period. The larvae grew on three kinds of semi-synthetic diet. The A-diet has more carbohydrate than the others, the B-diet has carbohydrate in 1 : 2 with protein, and the C-diet has more protein than the others. All the diets were kept at 16
during the 4th moulting period. Proteinase activity of digestive juice at the 5th day of the 5th instar was analyzed by Anson's hemoglobin method. Sucrase activity of midgut tissue at the 5th day of 5th instar was analyzed by Somogyi-Nelson's method. The results were as follows. 1. The dietary composition influencing contents of blood sugar was not related to the rearing temperature during the 4th moulting period. The contents of blood sugar appeared to increase in A-diet, B-diet and C-diet order, while proteinase and sucrase activity were stronger in C-diet, B-diet and A-diet order. 2. All kinds of diets showed almost the same fact that proteinase activity at 16
was stronger than that at 32
. 3. It was found that sucrase activity became gradualy stronger at 32
in order in all kinds of diets. 4. There was an interaction in proteinase activity between the dietary composition and the rearing temperature in male larval digestive juice during the 4th mouiting period. On the other hand, there was an inter-acion in sucrase activity between the dietary composition and the rearing temperature in both female and male larval midgut tissue during the 4th moulting period.
Studies on the Applicability of Furazolidone to the Silkworm Rearing Industry as a Useful Remedy for Certain Silkworm Diseases (Report I)
Journal of Sericultural and Entomological Science, volume 15, issue 1, 1973, Pages 15~25
The author studied the applicability of Furazolidone to the silkworm rearing industry as a useful remedy for certain silkworm diseases, at the silkworm rearing house of the college of agriculture, Seoul national university, during both the spring and the autumn silkworm rearing season of 1972. Discovering the fact that Furazolidone, when put on the mulberry leaves in a powdered form, is eaten along with the leaves by silkworms and thus the systematic administration of Furazolidone to silkworms is possible, the experimenter carried on a series of experiments (1. determining the in vitro antibacterial activity of Furazolidone to four pathogens of silkworm diseases-Bacillus thuringiensis, Aspergillus oryzae, Aspergillus flavus, and Isaria farinosa, 2. observing the prophylactic and therapeutic effect of Furazolidone against the experimental flacherie caused by inoculation of B. thuringiensis, and 3. examining the toxicity of Furazolidone to silkworm larvae). As the results of the experiments the investigator found out the fundamental fact that Furazolidone exerts a good prophylactic and therapeutic effect against flacherie which is the most common and important silkworm disease: Furazolidone, in in vitro test, inhibited completely the growth of B. thuringiensis, the pathogen of bacterial flacherie, at the concentration of 1
g/mι. with the tube method and at the concentration of 5
g/mι. with the plate method, and the drug showed an excellent prophylactic effect and a considerably good therapeutic effect, depending on the time of administration, on the 5th instar silkworms inoculated B. thuringiensis, at the tentative dose of 150mg. per 10 silkworms administered once a day for 2 days. For the practical administration of Furazolidone against flacherie, the dose, the time and duration of administration, and the form of preparation, will be investigated more closely.
A Study on the Estimation of Convenient Sample Size for Cocoon Reeling Test
Journal of Sericultural and Entomological Science, volume 15, issue 1, 1973, Pages 27~30
The present research has been carried out in an attempt to obtain fundamental data which contribute to enhansing the accuracy of cocoon reeling test and improving the method of it more effectively. The results obtained are as follows: 1. The standard deviation and coefficient of variation of percentage of reelability and of percentage of raw silk yield to the sample size tended to show a gradual decrease according to the increasing of the sample size. However, the significant differences in the standard deviation and coefficient of variation of percentage of reelability and of percentage of raw silk yield between the different sizes of the samples having more than 300 cocoons have not been observed according to their sizes. 2. The significant difference in the standard errors from percentage of reelability between the samples of 300 cocoons and 400 cocoons have not been observed. 3. The significant difference in the standad errors from percentage of raw silk yield between the samples of 300 cocoons, 500 cocoons and 600 cocoons have not been observed. 4. The significant difference in the standard deviation, the standard errors and coefficient of variation to the length of a bave between the different sizes of the samples have not been shown. 5. From the above result, it is estimated that the optimum sire of the sample for the cocoon reeling test is the 300 cocoons.
Reological Studies on Cocoon Filament III. Stretching Strength and Elasticity Index of Cocoon Filament in Stretching Transformation
Journal of Sericultural and Entomological Science, volume 15, issue 1, 1973, Pages 31~35
Strength, elongation and elasticity are essential properties with regard to the tensile properties of raw silk thread. The reasonable harmony of the above three physical characters appears to be the specific nature of the raw silk thread and the dynamical functions are determined by the cocoon filament forming the raw silk. From such viewpoint, the author has studied the tensile property of the thread when the cocoon filament is stretched and transformed. The results obtained are as follows: 1) The stretching strength of the inner layer filament is stronger than that of the outer and medium layer ones. 2) The stretching strength of the filaments is influenced by the moisture-heat denaturalization of cocoon layer sericin in the drying process. 3) The more transformed functions by the stretching become, the lower the elasticity index of the cocoon filament was shown. 4) When cocoon layer sericin is denatured by moisture and heat, elasticity index of the filament is decreased.
Studies on Raw Silk Cohesion for Promotion
Journal of Sericultural and Entomological Science, volume 15, issue 1, 1973, Pages 37~48
The purpose of this studies is to improve the cohesion of raw silk through various analyses on cocoon drying, cooking, reeling, re-reeling, and on the properties of water. Also we investigated the correlation between silk testing items which we have reached to the following results. 1. Drying of cocoon When cocoons were slowly dried with 100
, the results of cohesion became much better. On the other hand, the results were considerably decreased in case the temperature with 115
. 2. Cooking of cocoon In case of the cooking of cocoon, we found that the result of cohesion was best with incomplete cooking, that of the control was next, while in over cooking, the results were very low. Also the results of cohesion were much better when using the method of over cooking with sericin arrestive agent than that of incomplete cooking with sericin agglutinating agent. 3. Reeling of cocoon A) When the temperature of reeling bath was 25-45
, the results of cohesion test were much better, but at the temperature below 25
or above 45
, the results became worse. B) With out the process of croissieur, the results of cohesion were too bad, but in case of croissieur more than 1cm, cohesion became better rapidly. Further more, we understood that the results of cohesion were improving slightly with longer length of croissieur. C) When the velocity of reeling was increased, the results of cohesion also improved. The best results were shown when reeling velocity was 180-220 r.p.m. But when the velocity was increased more than 220 r.p.m., the results of cohesion got worse more or less. D) When the temperature of the drying pipe in reeling machine was raised, the results of cohesion also showed a tendency to improve. 4. Re-reeling A) We could net reach a conclusion as to have correlation between the number of dipping repeat in vacuum tank and the results of cohesion before re-reeling process. B) When we used Seracol 500 as an agglutination protective agent with l/1000 to l/2000 of water, the results of cohesion test were better. C) When we used Pearl-lite as an agglutination protective agent with 1/1000 to 1/2000 of water, the results of cohesion were considerably better. D) We gained tile best results when used Cohesion Improving Chemical, A-80, with 500-1500 times diluted. 1) Results of cohesion was improved when humidity was low or temperature was high in the rereeling machine. 5. Filature water A) The water pH near the isoelectric point of protein showed the best cohesion, but the farther water pH, the worser results were obtained. B) With the increasing of M-alkalinity in filature water, the results of cohesion were worse. Above all, we understood the tendency of the results of cohesion get worse when the M-alkalinity is increased above 200 ppm. C) By increasing the total hardness of the filature water, it improved the results of cohesion. Especially, when the total hardness was above 300ppm, the results were extremely high. 6. Effects combination of each results A) The result of effects combination in filature processes with the obtained best conditions was distinctively improved. But the results could not reach in mathematicaly double effect. When reelied under worse conditions, the results of cohesion test were too bad. There was "effect limit" for the promotion. B) Generally the results of cohesion were bad when the filature conditions(the temperature, pressure and the properties of water, etc) are processed as sericin loss to be high. On the other hand, the results were very good when lower sericin loss was controlled in filature conditions. C) When filature conditions such as reeling velocity and croissieur length provide pysical cohesion ability and when raw silk dry fast during reeling and re-reeling, we found the result of cohesion was better. 7. Correlation of silk testing items. A) A negative correlation exists between the results of cohesion test and cleanness defect. Another word, the result of cohesion test was found to be worse as cleanness defect increased. B) In cleanness, cohesion has negative correlation against the number of slugs, but we could not find any correlation against long loops, loose ends. C) Cohesion has negative correlation against average neatness and low neatness defect. The better the results of neatness respectively, the better the results of cohesion found. D) There is no correlation between tenacity and the results of cohesion test, but there was high positive correlation between the results of elongation and those of cohesion test. The more elongation, the better the results of cohesion was found.