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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 31, Issue 2 - Dec 1989
Volume 31, Issue 1 - Mar 1989
Selecting the target year
Studies on the Mulberry Graftages IV. Cold Hardiness in December
Lee, Won-Ju ; Lee, Ju-Won ; Park, Gwang-Jun ;
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 65~68
Cold hardiness of mulberry graftages was examined with two varieties, Kaeryangppong and Keomseolppong in early December. Exposure temperatures were three levels (-5, -10 and -15
) at the 4 exposure times (6, 12, 24 and 48 hrs). No damage showed at -5
for 48 hrs. Survival rate decreased sharply from -10
, regardless exposure times. There was no survivals from -10
for 12 hrs in Kaeryangppmong and from -15
for 6 hrs in Keomseolppong. This suggested Keomseolppong was stronger in cold than kaeryangppong. Regrowth decreased drastically from -10
Topping Effect on the Passed Rate of Mulberry Graftages and Regrowth after Planting
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 69~71
Experiments were conducted to investigate the effect of topping from mulberry graftages on the their size. Topping season was examined at 10 days intervals from July 15 to August 5 1986 and 1987 on the paddy and upland, respectively. The results were : 1. Topping increased the passed rate of graftages 3.0-15.9%, compared with the control. 2. Optimum season of topping fell around July 15. 3. Topping showed no difference to branch length, survival rate and shoot length in the next year. 4. There was no difference between paddy and upland nursery garden in the passed rate.
Studies on the Purification and Biochemical Properties of Vitellin in the Antheraea yamamai Guerin-Meneville I. Isolation and Purification of Vitellin and its Change to Embryonic Development
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 72~81
Antheraea yamamai vitellin was purified from matured eggs by polyacryamide gel electrophoresis, also stage dependent appearance, immunological comparison and relative content of the protein were investigated. 1. Vitellogenin, the precursor of vitellin, was first detected in the larval hemolymph at the late spinning stage by polyacrylamide gel electrophoresis and immunoelectrophoresis. 2. The electrophoretic mobility of the vitellin was identical with that of Bombyx mori and of Bombyx mandarina. However, the specific antiserum against A. uamamai vitellin did not react with either that of Bombyx mori or Bombyx mandarina in immumo-diffusion test. 3. Relative content of A. yamamai vitellin to the total soluble egg protein was 46.0 percent and did not change till eight days after oviposition. But the content started to decline from ten days after oviposition and was negligible in the five or serventeen month old eggs.
Studies on the Purification and Biochemical Properties of Vitellin in the Antheraea yamamai Guerin-Meneville II. Biochemical Properties of Vitellin
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 82~90
Antheraea yamamai vitellin was purified from matured eggs by polyacrylamide gel electrophoresis for characterization of its biochemical properties : molecular weight, sugar and lipid composition, amino acid composition and electron microscopic morphology, etc. 1. A yamamai vitellin was composed of two subunits, large and small, showing different mobility in SDS-polyacrylamide gel electrophoresis. 2. The molecular weight of the vitellin was estimated to be approximately 450,000 dalton and the large and small subunits were 174,000 dalton and 44,000 dalton, respectively. 3. The vitellin seemed to be a glycolipoprotein since it showed a positive reaction to coomassie brilliant blue, sudan black B and PAS staining. Both subunits were similiar in this aspect. 4. Lipid of the witellin reveraled several different types including saturated lipids. 5. When the vitellin was incubated at 7
for 60 minites its apoprotein still cross-reacted to the specific antiserum to the native vitellin. Its sugar components were also detected by PAS staining, but its lipid portion was not detected by sudan black B staining. 6. Its amino acid composition was similar to that of other insects, but its glycine content was peculiarly very high. 7. The vitellin molecule was spherical in shape with a diameter of 14
0.8nm by negatively.
Heritability, Genetic Correlation and Path Coefficient Analysis of Economic Characters in the Silkworm, Bombyx mori
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 91~101
This experiment was carried out to analyze genetic parameters for the selection of silkworm by diallel-crossing with three Japanese and four Chiness silkworm races. Heritability, genetic correlation and path coefficient were calculated as the statistical parameters to evaluate direct or indirect effects of various gentic characters on bave weight. The heritabilities of fifth instar period, cocoon weight, cocoon layer weight, cocoon layer ration, fibroin contents, boiling off ratio and bave weight were over 76.65% in both F1 and F2 generations. There were positive coefficients in both phenotypic and genotypic correlations between male and female of both F1 and F2 generations. The latter showed greater values in general. The correlation between bave weight and the characters of cocoon weight, cocoon layer weight, cocoon layer ratio, fibroin contents were over 0.727 in both sexes of F1 and F2. Therefore, these results suggest that above characters would be useful for genetic selection of silkworms. As result of path coefficient analysis, there was a direct effect with path coefficient value of 0.991 between bave weight and F1 female cocoon layer weight, and with the value of 0.803 between bave weight and F1 male cocoon weight. In F2 the effect showed 0.938 between bave weight and male cocoon weight and cocoon layer weight had greater influence on direct or indirect effects (in path coefficient) than the others.
Classification and Selection of the Breeding materials in the Silkworm, Bombyx mori, by Multivariate Analysis 1. Classification of the Silkworm Genetic Stocks by Principal Component Analysis and Cluster Analysis
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 102~112
Principal component analysis and cluster analysis were performed on the nine quantitative characters of the one hundred and forty eight silkworm genetic stocks. The six major quantitative characters such as cocoon yield, cocoon weight, cocoon shell weight, cocoon shell percentage, larval period of the 5th instar silkworm, and total larval period showed significantly positive correlation between them. The first three principal components extracted form the initial nine variables by principal component analysis accounted for about eighty percent of original information. The first and second principal components were characterized as factors related to silk productivity, and cocoon productivity, respectively. On the basis of multivariate analysis using city block distance determined from the first three principal components to measure the phenotypic diversity, the one hundred and forty eight silkworm genetic stocks could be clustered into seven varietal groups, and the phenotypic diversity between the varietal groups was partly related to their geographical origins. Among 7 varietal group, group II and IV revealed higher silk and cocoon productivity.
Characterization and Taxonomic Classification of S85, a new Microsporidia, isolated from the Silkworm, Bombyx mori L.
Jo, Se-Yun ; Son, Hae-Ryong ; Im, Jong-Seong ;
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 113~120
A new microsporidia (S85) was isolated from the silkworm, Bombyx mori L. in Suwon, Korea in 1985. The electron microscopic observation of the fine structure of the spore was studied for the characterization of the new microsporidia and its taxonomic position. The sporewall was composed of three different electron dense layers and the spore surface was rouph and wrinkled. The polaroplast has two parts which differed in the arrangement of the lamellae. The polarfilament was isofilar with thirteen coils and it was consisted of eight concentric layers of different electron density. The spore contained a single nucleus and the pansporoblast appeared as a thin ballon-like structure surrounding the spore. The membrane was generally smooth and shown occationally wrinkled.
Effects of pH and Hardness Resulted from Total Carbonate Concentration on Sericin Solubilities
Nam, Yeong-Rak ; Chae, Dae-Seok ; Seong, Jae-Cheon ;
Journal of Sericultural and Entomological Science, volume 31, issue 2, 1989, Pages 121~126
Two kinds of solution for the measurement of solubilities of Sericin are prepared as followings at temperature 90 deg. C. One has the total carbonate concentration as 0, 50, 100mg CO2/l prepared with non-carbonate distilled water, sodium hydrogen carbonate and 0.1N HCI and NaOH, the other has total hardness, that is, calcium hardness or magnesium hardness as 0, 20, 50, 100mg CaCO2/l respectively prepared with non-carbonate distilled water, calcium carbonate and magnesium oxide. Solubilities of Cocoon layer Sericin at above solution gives following results ; 1. pH shows little effect on the solubility of Sericin at the non-carbonate solution but at the carbonate solution pH shows a sensitive effect on the solubility of Sericin. These means that pH controls the concentration of H2CO3, HCO3-and CO32- which prevent and promote the solution of Sericin. 2. After the cocoon layer treatment at the solution, the initial pH of 4.0, 7.0, 9.0 of the solution changed to 6.0-6.5 at the lower total carbonate solution. However in the higher total carbonate solution pH did not changed very much. This may be explained by the buffer action of carbonate. 3. The effect of the hardness on the solubility of Sericin was not found in the non-carbonate solution with the standard hardness after treatment of cocoon layer.