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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 33, Issue 2 - Dec 1991
Volume 33, Issue 1 - Jun 1991
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Change in Soluble and Insoluble Proteins during Embryonic Development of the Silkworm, Bombyx mori
Journal of Sericultural and Entomological Science, volume 33, issue 1, 1991, Pages 1~5
PBS soluble- and insoluble-extracts form silkworm eggs were analysed by native-PAGE and SDS-PAGE. During the embroyonic development, soluble proteins which are mainly composed of ESP, vitellin and 30K proteins showed similar degradation pattern in both electrophoretic analyses. Several peptides which seemed to be intermediated forms of yolk proteins were detected in latter part of embryogenesis, while a protein band newly appeared in one day elapsed after acid treatment. When insoluble extracts from silkworm eggs were analysed with SDS-PAGE, several peptides were detected at the later stages of the embryonic development, and newly hatched larvae. These peptides are considered to be structure proteins for embryonic morphogenesis.
Studies on the Mulberry Graftages VI. Spray Effect of Ethrel (2-chloroethylphosophonic acid) on the Graftages Quality
Journal of Sericultural and Entomological Science, volume 33, issue 1, 1991, Pages 6~8
The study was carried out to investigate the effect of ethrel (2-chloroethylphosophonic acid, 39%) on the graftages quality. Ethrel was sprayed 5 days interval from October 10 to October 30 at 6 places (Suwon (I), (II), Chungju, Kongju, Wanju, Jangsong). Spraying conentration was 1, 000 ppm (400 times dilution). 1. Spray took 3.3 hrs and 500 m of ethrel on 10a eara. 2. The passed rate of graftages decreased 5-8% on the treatments earlier spray than on October 20. 3. New shoot length and number of shoot of the ethrel treatments were the same as the natural leaf falling.
A study on the dyeing-rate in single and mixture dyeing of silk with levelling and milling acid dyes
Journal of Sericultural and Entomological Science, volume 33, issue 1, 1991, Pages 9~12
A study on dyeing-rate for single and mixture dyeing of silk with acid dyes was carried out. Single and mixture dyeings were performed with levelling and milling dyes at 7
and at pH 3, pH 4 and pH 5, respectively. The results of this study were summerized as follows : 1. C. I. Acid Orange 7. A) At pH 3, dyeing-rate was more fast in the single dyeing than in mixture dyeing at the beginning of dyeing process. B) At pH 4 and 5, dyeing-rates tend to get very similar in single and mixture dyeing. 2. C. I. Acid Blue 138 A) At all pH value, dyeing-rates were more fast in the mixture dyeing than single dyeing at the beginning of dyeing. B) As the time increased, the difference of dyeing-rate and adsorption amounts between single and mixture dyeing was not shown.
Characterization of Flacherie Virus of the Silkworm, Bombyx mori, isolated in Korea
Journal of Sericultural and Entomological Science, volume 33, issue 1, 1991, Pages 13~20
The flacheric virus(FV) was isolated from the diseased larvae with flaccidity symptoms of the silkworm, Bombyx mori, which were collected in Hyangnam, Kyunggi Province and in Youcheon, Kyungpook Province. The properties of the virus were investigated and compared with the Japanese FV in morphology, size, nucleic acid and structural proteins. The Hyangnam isolate had a diameter of 27
1, 7nm with speherical shape and contained RNA of which the electrophoretic pattern was same as that of the Japanese FV. The virus had four sturctural proteins and their molecular weight were estimated as 35, 000, 33, 000, 31, 000, and 11, 400 daltons, respectively. The Youcheon isolate had two different sizeds in diameter of 27
1.7nm and 21
0.8nm. The antigenicity of the Hyangnam isolate was proved to be identical to that of Japanese FV, whereas the antisera against the Youcheon isolate (mixed with two different sizes) reacted with Japanese FV and densonucleosis virus type 1, respectively. From these characteristic of the isolated viruses, it was concluded that the Hyangnam isolate was identical to the Japanese FV and the Youncheon isolate contained the same viruses as the Japanese FV and DNVI.
Polyhedral Protein Synthesis and DNA Replication of Bombyx mori, Nuclear Polyhedrosis Virus in a B. mori Cell Line
Journal of Sericultural and Entomological Science, volume 33, issue 1, 1991, Pages 21~26
Bombyx mori nuclear polyhedrosis virus (BmNPV) was successfully multiplied in the nuclear of BmN4 cells cultured with insect Grace's medium. By electron microscopic observation, the virons had a single nucleocapsid in an envelope. Polyhedral protein synthesis of BmNPV in BmN4 cells was detected at 18 hr p.i. and polyhedral protein was a singlepolypeptide with a M.W of 30 kd. At 48 hr p.i. polyhedra formation was observed by inverted mociroscope and electron microscope. Genome analysis of BmNPV by restriction endonucleases was not revealed the difference between virus produced in vivo and that in vitro.
Morphological Characteristics and Infectivity of Microsporidian Isolates Derived from Yellow-hind Winged Arctiid, Eilema griseola (Hubner) and Cutworm, Agrotis tokionis Butler, to Silkworm Larvae
Journal of Sericultural and Entomological Science, volume 33, issue 1, 1991, Pages 27~31
Microsporidian spores were isolated from yellow-hind winged arctiid, Eilema griseola (Hubner), and cutworm, Agrotis tokionis Butler, and their morphological characteristics and their indectivity to silkworms were investigated. The shape of the microsporidian isolates was oval and the sige of the spore isolated from yellow-hind winged was measured in 2.6
and that of the isolated from cutworm was measured in 3.7
. Both the isolated microsporidians showed the infectivity to silkworms, but the isolates did transovarian transmission in silkworms and the silkworm moth infected with the isolate from yellow-hind winged laid eggs irregularly.
Purtification of Parasporal Protein Crystals of Bacillus thuringiensis
Kim, Yeong-Hun ; Kim, Sang-Hyeon ; Gang, Seok-Gwon ;
Journal of Sericultural and Entomological Science, volume 33, issue 1, 1991, Pages 32~36
The study has been carried out to acquire some basic informations about Bacillus thuringiensis for developing the microbial pesticide. Three strains of Bacillus thuringiensis var. Kurstaki, dendrolimus and aizawai, were used in the experiments as follows. Growth characteristics of each strain were examined and parasporal protein crystals were isolated from the mixtures of spores and protein crystals by the new method of centrifugation in two-layer cushion of Renograffin using a fixed angle rotor. The results are as follows. 1. No difference was shown in growth characterestics among three strains of B. thuringiensis. In growth curves, all strains reached to exponential phase by 2 hr and stationary phase by 7-8 hr after inoculation. 2. The pH of the culture media during exponential growth stage decreased about 1.4 of a pH unit at the beginning of sporulation, but recovered during the early stage of sporulation and then remained nearly constant during the later stage. 3. As 10
sample was applied to two-layer cushion of Renograffin and then centrifuged for 1hr at 27,000g a fixed angle rotor, the purity and recovery ratio was 99.9% and 5.8%, respectively. It has been shown that the new method for the isolation of parasporal protein crystals was more efficient than any from the estabilished methods.