Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 34, Issue 2 - Dec 1992
Volume 34, Issue 1 - Jun 1992
Selecting the target year
Long-term Preservation of Bombyx mori Stocks by Frozen Gonad Storage
Kim, Sam-Eun ; Seong, Su-Il ; Lee, Sang-Mong ;
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 1~7
For a long-term preservation of silkworm stocks by frozen gonad storage, fundamental topics such as freezing rate and transplanting stage of the gonad, proper cryoprotectant, and super-cooling temperature and freezing point of the freezing medium were examined and following results were obtained. Proper method to anesthetize the ovary-recipient silkworm was to dip the animal to cold water for 10 minutes, and the ovary taken from the 4th instar larvae was more suitable for freezing-preservation than that from the 5th. Concerning the cryoprotectant, glycerol and DMSO were effective to prevent cryoinjury of the ovary, but sorbitol was not. The supercooling temperature and freezing point of the medium to freeze the ovary and testes were checked, and consulting with the results desirable cooling rate was confirmed. On the desirable conditions of transplanting methods, freezing rate and cryoprotectant concentration ect., the next generation was obtained when the females implanted frozen-thawed ovaries mated with normal males, but none of the normal females mated with the males implanted frozen-thawed testes laid fertilized eggs. Now it is needed to improve the connecting ration of the ducts associated with the transplanted testis to those of the hosts.
Effects of Application of Animal Feces to the Mulberry Field on the Chemical Properties and Mulberry Yield
Chu, Jae-Won ; Kim, Nak-Sang ; Yu, Geun-Seop ;
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 8~14
To investigate the effects of applying animal feces to the mulberry field on the chemical properties and mulberry leaf yield, poultry, pig and cattle feces were applied to the mulberry field making-up the sand loam from 1988 to 1990. The chemical properties of the mulberry field have been improved by the application of the poultry, the pig, and the cattle feces, increasing pH level, organic matter, P2O5 and exchangeable cation, especially Mg and K. Applying the pig feces and poultry feces to the mulberry field increased leaf yield during autumn rearing season, but not increased during spring rearing season. Applying animal feces to the mulberry field increased the content of total-cargbohydrate in the leaf in autumn.
Regulation of Haemolymph Juvenile Hormone Esterase Activity in Larvae of the Silkworm, Bombyx mori
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 15~20
Effects of starvation, ligation, refeeding and methoprene treatment in the feeding phase of the fifth instar larvae of Bombyx mori on the regulation of juvenile hormone esterase(JHE) activity were investigated. Starvation and ligation contributed to the reduction of JHE activity, however, JHE levels in starved larvae were slightly higher than in legated larvae. Haemolymph JEH activity of starved larvae was increased by refeeding, and duration of increasing time of JHE activity after starvation was related to duration of starvation. When starved larvae were applied methoprene topically, JHE activity were not changed at day 0 and 1, but were increased by 1.3-1.4 times between day 2 and 5. When ligated larvae were applied methoprene topically, JHE activity was not changed at day 0, but were increased by 1.9-2.3 times between day 1 and 5. These results suggest that head factor, juvenile hormone(JH) and nutrient are major factors in the regulation of JHE in the feeding phase of the fifth instar larvae of Bombyx mori. Especially JHE might be regulated by the co-operative action of head factor and JH. However, head factor plays important role in the early stage, while JH plays important role thereafter.
Analysis of Gene for Major Quantitative Characters in Silkworm, Bombyx mori
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 21~29
In the experiment, gene actions were analyzed for seven silkworm varieties in order to obtain basic information on their genetic improvement by diallel crosses. The results obtained were summarized as follows : in analysis of variance, significantly maternal and reciprocal effects were observed for all character except female cocoon shell percentage and female pupal weight. Over-dominance was shown by Vr-Wr graphic analysis in three characters such as female cocoon weight, female and male pupal weight, and partial dominance in five characters such as male cocoon weight, cocoon shell percentage in both female and male. Component of genetic varience analyzed for five characters such as male cocoon weight, cocoon shell weight, cocoon shell percentage in both female and male showed that additive effects were higher than dominant effects, and female cocoon weight showed dominant effects higher than additive effects. Heritability of narrow sence was higher more than 0.51 and heritability of broad sence was higher more than 0.77 in all characters except for female pupal weight. The directions of dominance were negative for female cocoon shell weight and somewhat higher for female and male cocoon shell percentage. Meanwhile cocoon weight, pupal weight in both female and male showed positive in the direction of dominance.
Physiological and Biochemical Studies on the Adult Heamolymph Proteins in Lepidoptera II. Purification and Molecular Properties of Adult Specific Protein in the Silkworm, Bombyx mori
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 30~34
Isolation, purification and molecular properties of adult specific protein (ASP) were investigated in the silkworm, Bombyx mori. ASP was purified from adult haemolymph by gel filtration on Sephadex G-100 and ion exchange chromatography on CM52. The preparation was shown to be homogeneous by native-PAGE and immunoelectrophoresis. The purified protein was consisted of two different subunits with molecular weights of 19.5kDa and 17.5kDa, and contained no sugars and no lipids.
Enzyme-Linked Immunosorbent Assay(ELISA) for the Rapid Detection of the Flacherie Virus Disease
Gang, Seok-U ; Kim, Gwon-Yeong ; Gang, Seok-Gwon ;
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 35~40
An enzyme-linked immunosorbent assay (ELISA) was studied for the rapid diagnosis of the flacherie virus (FV) of the silkworm, Bombyx mori. The optimised concentration of rabbit anti-FV IgG and enzyme conjugate for the this technique were 15
and 1:100 dilution, respectively. In ELISA, the detectable concentation of purified FV was 15ng/
, and the flacherie viral antigens in the larval extracts were detected as early as 24 hours after the experimental infection. The results indicated that ELISA technique proved to be applicable for the rapid diagnosis of flacherie virus disease.
Degradation of the Parasporal Crystal Proteins of Bacillus thuringiensis in Gut Juice of Some Insects
Kim, Yeong-Hun ; Kim, Sang-Hyeon ; Gang, Seok-Gwon ;
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 41~48
Dissolution and degradation of the parasporal crystal proteins produced from B. thuringiensis var. kurstaki, B. thuringiensis var. dendrolimus and B. thuringiensis var. aizawai were investigated. SDS-polyacrylamide gel electrophoresis analysis showed that the crystals contained major protein with molecular weight of approximately 134 kDa for B. thuringiensis var. aizawai 143 kDa for B. thuringiensis var. kurstaki and 149 kDa for B. thuringiensis var. dendrolimus, respectively. Crystals of three other strains were incubated alkali solutions at various pH or gut juice of Silkwarm Bombyx mori, Fall webworm Hyphantria cunea, and Common Cabbage worm Pieris rapae. When crystals of these strains were solubilized by alkali solutions, no major differences among strains B. thuringiensis could be detected. Among the strains studied, crystal protins (130-66 kDa) consist of protease resistant polypeptides in the 45-66 kDa size range when treated with gut juice of three insect species.
Effects of Methacrylamide Treatment on Silk Fibers II. Thermal Behavior of Methacrylamide-treated Silk Fibers
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 49~56
Treatment of vinyl monomers onto silk fiber modifies the properties of the original silk fiber considerably. This field has been the subject of investigation by many workers using chemical and radiation initiation. Many studies on the reaction conditions, polymerization mechanism, physical properties and practical performances of methacrylamide-treated silk fiber have been continued. However, the polymerization mechanism has not been clearly revealed yet and this remains ambiguously whether the grafting is formed on fiber or not. In general, it has been accepted that free radicals were formed and vinyl monomers were polymerized in silk fibroin by graft polymerization mechanism, while active sties were varied by the types of monomer and initiator as well as by the reaction conditions. On the other hand, there is another argument on polymerization mechanism, in which monomers are polymerized and impregnated in the internal side of the fiber by homopolymerization. Though a large number of analytical methods are used to examine the polymerization mechanism of methacrylamide-treated silk fiber, the results on the basis of thermal analysis are merely reported in this paper. In differential scanning calorimetry (DSC) analysis, the thermal decomposition behaviors of the methacrylamie-treated silk fibers were determined and compared to those of the controlled silk fibers. DSC curves obtained from the methacrylamide-treated silk fibers showed double peaks at around 290
(A peak) and 320
(B peak) which are attributed to the thermal decomposition of the methacrylamide polymer and silk fibroin fiber, respectively. The temperature of A and B peak shifted to higher value with the increase of add-on. Also, the moisture regain of the treated silk fibers increased with add-on.
Comparison of the Electronic Methods of Raw Silk Testing and Grading with Those of the Current Methods I. Defference in Inspection Results and Grading to the Electronic Methods
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 57~71
These studies were carried out to establish Korean opinion about "The electronic method of raw silk testing and grading" which was taken shape along with the report by Silk Standard Committee(S.S.C.) at the meeting of International Silk Association(I.S.A) Directing Board session held in Zurich Swiss. This establishment was througly examine whether or not the above are the essential factors to be fulfilled in force as a international standard method of raw silk testing and grading.d grading.
Comparison of the Electronic Methods of Raw Silk Testing and Grading with Those of the Current Methods II. The Effectiveness and Convenience of Raw Silk Inspection Process by Uster Tester III
Journal of Sericultural and Entomological Science, volume 34, issue 1, 1992, Pages 72~80
These studies were brought about to know how much the elctronic methods of raw silk testing is correct, effective and convenient in the testing process. These effectiveness and convenience for the testing process under the emergency of the automatic system in raw silk inspecting process, the convenience of inspection in crease in it's capability decreasing the necessary working members, but as certain defect, the corresponding time span needs more than the former case in kind. Judging from the above mentioned carefully, it is necessary or even immidiate for participants to deeply study and search in earnest as well, before adopting the so called International Method of Raw Silk Test and Classification which was proposed by S.S.C in 1990.