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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 35, Issue 2 - Dec 1993
Volume 35, Issue 1 - Jun 1993
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Activation Mechanism of Protease in the Germination of Mulberry Seeds
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 1~6
The activity change of mulberry seeds protease was compared during germination for 5 days at 28
in the dark place after daily hormone injection of different concentration. The protease from germinated mulberry seeds for 4 days was partially purified and the enzyme characteristics was investigated. The protease activity of mulberry seeds treated by hormone was highest with 10
m GA3 followed by 10
M zeatin and 10
M kinetin. The protease activity of mulberry seeds was increased by 14% with 10ml agar culture that control at 4th day of germination. The protease from mulberry seeds was purified 313 fold by DEAE-Toyo-pearl 650M, Butyl-Toyopearl, Hydrozylapatite and Toyopearl HW 55M. After purification, the specific activity of the enzyme was 175 units/mg. Optimum pH and temperature of protease from mulberry seeds was 5.0 and 37
, respectively. The protease was stable below 37
and the enzyme activity was decreased by 50%, when incubated at 52
for 10minutes. The protease activity of mulberry seeds was inhibited by metal ions such as mercury, iron, zinc, copper, but activited by magnesium, choromium, aluminium ions. The Km value of the protease was 0.89mM with azocasein as a subscribe.
Studies on the Mulberry Graftages VII. Earlier Grafting with Preservation of Grafts in Sawdust
Lee, Won-Ju ; Choe, Yeong-Cheol ; Lee, Yeong-Han ;
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 7~10
Mulberry grafting season in normally a one month period starting at the end of March. Such a short period creates a shortage of qualified grafters and inflates wages. Pine tree sawdust was tested as a medium in which to preserve graftages. Sawdust was moistened to two ratios of water to dry weight of sawdust (1.5 and 2.0). Scions were either grafted the same day they were collected or first incubated for 10days at 15
. Grafting period could be extended to the middle of February under 1.5 times moisture content and fresh scions. Preservation of scions increased water content, T-N, P2O5, CaO and inositol, whereas decreased total C, glucose and fructose. With no preservation total C decreased, inositol increased slightly early March followed sharp decrease, and sucrose increased with time.
Reduction of Phosphorus Fertilizer in Mulberry Fields, High in Phosphate
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 11~16
Field experiment and silkworm rearing were conducted for 4years in an attempt to reduce phosphorus fertilizer in mulberry field with high levels of phosphate. Experiments consisted of four treatments; normal recommended P application(13kg P2O5/10a), half, null and no fertilizer on each of 3 mulberry fields, two fields with 300ppm and one filed begining with 450ppm of aviable P2O5 content in soil. One of 300ppm fields was used for leaf quantity and one for leaf quality test by silkworm rearing. Leaf yield in the 300ppm field decreased after the 3rd spring in the no phosphorus treatment and in the 4th fall in the half phosphorus treatment. No yield decrease occurred in 450ppm field for 4 years. Yield decrease did occur in the next rearing season after soil phosphate content in the 10~20cm zone dropped below 150ppm. Phosphorus fertilizer should be applied at this time. Though P2O5 concentration in leaves of no phosphorus treatment was 0.18% lower that that of conventional treatment, there were no difference in cocoon quality.
Influence of Late Summer Fertilizer on the Yield and Cold Damage of Mulberry in Kangwon Province
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 17~20
Cold damage of mulberry in Kangweon Province is generally higher that of other provinces. Farmers suspected cold damage caused from late summer fertilizer. To understand the effect of late summer fertilizer. To understand the effect of late summer fertilizer, two additional summer fertilizer times, August 10 and 20, were compared with conventional time(June, 20). Mulberry yield increased by 10~30% in 1m middle cut, and 10~17% in leaf picking method in August 10 treatment, compared with conventional. Additional summer fertilizer did not increase cold damage rate of branches. Leaf yield in leaf picking was higher by 6~29% that that of 1m middle cut.
Studies on the Mulberry Variety "Yongchonppong"(Morus alba L.) II. Increasing Effect of Mulberry Leaf Value for Food by Cover Rearing with Vinylon Gauze
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 21~27
Yongchonppong is liable to withering of leaf and it is lowering leaf value for the feed. In this respect, covering of the vinylon gauze over the feed has been carried out in the branch rearing system to sustain leaf value as itself. The covering of the vintlon gauze over Yongchonppong marks 7% and 9% increment of cocoon yield in dry season, spring rearing season, as compared to the non-covering of the vinylon gauze over Kaeryangppong and Yongchonppong, respectively. There was no significant differences in cocoon shell percentages of them. In more humid environment than average relative humidity in Korea the rearing results of "Yongchonppong" was improved by covering with vinylon gauze after feeding compared with the non-covered rearing. However, the vinylon guage covering was not effective for improving the rearing results of "Kaeryangppong". No effect of the vinylon gauze covering was seen in the area where it frequently rains and raining season lasts. In case of the non-covering of the vinylon gauze, Yongchonppong reduces repidly in the first three hours of exposure to the air, as compared to Kaeryangppong. The water content reductions rate of Yongchonppong in 6 hours of exposure to the air marks 7% to so when the dry vinylon gauze cover and 17 to 26% when the wet vinylon gauze covers, as compared to 58.8% of it for the noncovering of the vinylon gauze.f the vinylon gauze.
Effect of Juvenile Hormone Analogue(JHA) on the Adult Development of the Silkworm, Bombyx mori
Seong, Ju-Il ; Im, Bong-Hak ; Gang, Hyeon-A ;
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 28~35
Treatment of juvenile hormone analogue(JHA) at the 5th instar larvae prolonged the duration of adult development one to one and half days as well as the elongation of feeding time with the increasing of larval body weight. Morphological observation and protein analysis in hemolymphs, integuements, alimentary canals, fat bodies and ovaries also revealed that the development of these tissues and organs for adultation are affected by the JHA treatment.
Expression of Fusion Products of Insecticidal Crystal Protein Genes from Two Different Bacillus thuringiensis Strains
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 36~42
Expression of insecticidal protein by fusion product of truncated HD-1[CryIA(a)] N-terminal and HD-73[CryIA(c)] C-Terminal fragment of Bacillus thruingiensis subsp. kurstaki was investigate. Immunological analysis of transformants by using polyclonal antisera raised against the whole-crystal protein of HD-1 revealed that SK4 and SK5 were observed cross-reaction with polypeptides of 77-kDa and 105-kDa, respectively. Bioassay of the transformant pSK5 to Plutella maculipennis and Heliothis assulta were 96% and 97%, respectively.
Studies on the Scouring Effects of Scouring Soap Made from Rapeseed Oil
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 43~47
Using the new scouring soap made from rapeseed oil, various scouring test was done souring effects of new scouring soap were estimated. In the effects of scouring soap on the degumming ratio, OS (the soap made from oleic acid) was the most excellent and the next was RS (the soap made from rapeseed oil). Both OS and RS were more excellent than MS (the soap on the market). In a buffer power of soap solution, OS and RS were good, but MS was poor. The uneven dyeing was not observed in the silk fabric degummed by OS and RS, but observed in silk fabric degummed by MS. The value of tenacity and elongation in the silk degummed by OS and RS was higher that degummed by MS. In the crease recovery, softness and whiteness, the order of value was OS, RS, MS
Studies of Low Temperature Dyeing on Silk Fiber II. Low Temperature Dyeing of Silk Fiber with the Complex Dye
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 48~51
Using the new type auxiliary(K-1), dyeing experiment was done at the various dyeing conditions and the effects of auxiliary on the dyeing properties of metal complex dye for the silk fiber were dicussed. The exhaust rate was higher in the auxiliary 3% o.w.f. addition than sodium sulfate 50% o.w.f. The decreasing ratio of exhaust rate according to dye concentration increasing was lower at auxiliary addition than auxiliary non-addition. The exhaust rate according to increasing dyeing temperature was higher in the auxiliary addition than non-addition.
Physicochemical Characteristics of Silk Fibroin Degummed by Protease in Bacillus licheniformis II. Effect of Heat Treatment onto Degummed Silk Fiber
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 52~59
Raw silk degumming, by which the sericin and other marterials are eliminated from fibroin, is very essential process to produce silk fabrics. Alkali chemicals and enzymes have been used for the silk-degumming process. In this paper, the effect of heat treatment was investigated on silk fibers degummed by two different methods, soap and enzymatic degumming method. The difference between these two degumming methods was analyzed on the basis of results of mechanical testing, thermal analysis and intrated spectroscopy. The tenacity and the elongation of silk fiber are decreased by the heat-treatment in wet state. This tendency is observed in both cases of two degumming methods. The peak temperature in DSC analysis, which is attributed to thermal decomposition of silk fiber, was shifted to higher value with the heat-treatment temperature for the soap degummed silk fiber, however, it was not for the enzymatic degummed one. The IR crystallinity of soap digummed silk fiber is increased with the heat-treatment temperature, while that of enzymatic degummed fiber is not.
Physicochemical Characteristics of Silk Fibroin Degummed by Protease in Bacillus licheniformis II. Behavior in Aqueous Solution of Silk fibroin
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 60~68
It has been known that the silk degumming treated by hot alkali solution is easy to handle but is liable to yield poor-quality silk due to the degree of degumming loss, incomplete-degumming or over-degumming. Therefore, many studies have been carried out on the silk degumming by enzyme in order to improve the quality of silk. However, no attention has been paid to the physicochemical analysis of enzymatic degummed silk. In this paper, two different degumming methods, soap and enzymatic, are compared in aqueous solution state of silk fibroin. The results can be summarized as follows: There was no significant difference between two solutions on the bases of polarizing microscopy, TEM observation and SDS-PAGE. Spherulite of silk fibroin was not observed in polarizing microscopy, however the leaf-shape fibril structure was developed upon solidification. The size of spherulites of silk fibroin in TEM observation were 30~120nm with a wide range of size distribution. The intrinsic viscosity of enzymatic degummed fibroin solution was lower than that of soap degummed solution. This can be explained that the silk fibroin was more degraded by enzymatic degumming method compared with the soap degumming method. SDS-polyacrylamide gel electrophoresis showed that the fibroin molecule was composed of large component of molecule weight above 50 kd and small component of molecule weight about 20 kd. There was no difference in crystallinity between two degumming methods on the bases of results of DSC thermograms and IR spectra.
Effect of Testosterone Propionate on the Economic Traits of the Silkworm, Bombyx mori L.
Magadum, V.B. ; Magadum, S.B. ;
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 69~72
Effects of topical application with 1, 5 and 10
g/ml testosterone on the precocooning and post cocooning traits of the polyvoltine. Pure Mysore breed of silkworm were studied. Each concentration was treated independently at 36th to III, IV and V instar larvae(Treatment-1), at 48th to IV and V instar larvae(Treatment-2_ and at 72th to V instar larvae(Treatment-3). The repeated application with each concentration of testosterone propionate in treatment, and 2 larvae or single application in treatment 3 variously affected the precocooning and post cocooning traits. Repeated applications of all doses of testosterone propionate(Treatment-1) and single application of 1 & 5
g of the chemical(Treatment-3) increase the fecundity and decrease the percentage of cocooning and moth emergence.
A Marking Technique for Exorista bombycis(Louis) (Diptera:Tachinidae) by Adding Dye to the Adult Diet
Swamy, K.C.Narayana ; Devaiah, M.C. ; Govindan, R. ;
Journal of Sericultural and Entomological Science, volume 35, issue 1, 1993, Pages 73~76
Adding a non-fluorescent dye, Rhodamine-B, to the adult diet of uzi fly, Exorista bombysis (Louis) has shown to be a useful method for marking the eggs for flight range experiments. The method is timesaving and the dye is safe to handle and the marked eggs are easy to detect. Files fed on the diet added with dye did not have much negative effect on adult mortality and fecundity, but egg hatchability was affected.