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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 37, Issue 2 - Dec 1995
Volume 37, Issue 1 - Jun 1995
Selecting the target year
The Fertility beween Morus nigra L. from Turkey and Indigenous or Cultivated Mulberry in Korea
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 1~5
The fertilities of indigenous and cultivated mulberry species(varieties) which were polli nated with Morus nigra L. brought from Turkey were investigated. The percentage of ripening sorosis was very high in indigenous species, Morus tiliaefolia Makino and cultivated variety, Keomseolppong and it was around 50% in both Daeryukppong and Cheongilppong However, the flowers of Morus bombycis Koidz. (BOM5-8) and Yongcheonppong fell after pollination. Looking at the sizes of seeds, they are also various. Generally, it was extremely small in Morus tiliaefolia, but it was medium in Keomseolppong, Cheongilppong and Daeryukppong. The fertilization percentage was generally low with 32-42% in the varieties, but it was comparatively high in Keomseolppong and Morus tiliaefolia. The seed germination percentages of Daeryukppong and Morus tiliaefolia are 88.0% and 23.6%, respectively, but it was below 2% in Cheongilppong and Keomseolppong. The cross success percentages are 14.2% and 8.0% in Daeryukppong and Morus tiliaefolia, respectively, but it was below 0.3% in Cheongilppong and Keomseolppong.
Effect of Medium Conditions on Adventious Bud Formation of Mulberry Winter Bud Leaflet
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 6~9
To establish a regeneration system of mulberry through the adventitious bud formation of winter bud leaflet, medium conditions were examined. Adventitious bud formation rate of mulberry winter bud leaflet on the medium containing different concentrations of BA and TDZ as the growth regulators was high on 5mM BA and 0.5mM TDZ. The effect of preculture on the medium containing BA or TDZ was affected not only by the concentration of growth regulators but the duration of preculture. Fructose as carbon source was effective on adventitious bud formation.
Genetic Structure of the Mulberry Silkworm Population in Sri Lanka: I. Estimation of Combining Ability and Heritability
Lea, Ho-Zoo ; Alwis, Siriani-M.de ;
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 10~15
Genetic characterization of Sri Lankan silkworm bivoltine population has not been at-tempted so far, since its sporadic introduction of bivoltine strains into the island, starting from the 1950's. Genetic structure of Sri Lankan population of mulberry silkworm Bombyx mori was investigated through estimation of general (GCA) and specific combining ability(SCA) and heritability(
), on the economic quantitative characters from leading 8 inbreds and their 28 F1's in a half diallel cross, in an attempt to utilize the estimates in determination of future breeding methods and to predict the breeding value over the phenotypic value. It was found that the breeding population of the bivoltine silkworm in Sri Lanka has still maintained considerable amounts of additive gene action as well as nonadditive. For some time in the future, both breeding strategies of "selection without inbreeding" and also "inbreeding followed by crossing" should therefore be effective in genetic improvement of economic characters investigated. In addition, superior combiners in general and in specific F1′s were identified for each of 6 economic characters, to be immediately utilized in selection and also in cross breeding programs in Sri Lanka.
RFLP Analysis of Silkworms for DNA Polymorphism
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 16~26
DNA restriction fragment length polymorphisms(RFLPs) were used for the classification of 22 leading silkworm races and wild silkworm, Bombyx mandarina. A genomic DNA library from silkworm was partially constructed and was prescreened to evaluate the selected DNA probes. Three DNA probes (SP1-13, SP1-28, 10-42) were selected to determine the polymorphism between silkworm races. As a result, high polymorphism with the probe SP1-28, moderate polymorphism with SP1-13 and monomorphism with 10-42 were obse-rbed.
Studies on Food Habit Mutation in the Silkworm (I). The Origin and Characteristics of Polyphagous Strain Fb in Bombyx mori.
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 27~32
Studies were carried out to report breeding procedure and characteristics of Fb, a strain of silkworm with abnormal food habit polyphagous. Selection was continued up to the 14th generation toward higher incidence of cabbage leaves feeding variants. In the autumn of 1992(7th generation), selected strain were subjected to severe selection by feeding them on artificial diet that did not contain mulberry leaf powder. The results were clearly positive and reached to higher than 95% of cabbage feeding in the 14th generation. This strain was first discovered by feeding cabbage leaves, but later they were observed to eat some kind of plants like lettuce, Chinese cabbage, radish, beet, apple, persimmon, pear, etc. As described above the procedure, cabbage feeding trait was clearly heritable. The strain responsible for this traits was named Fb.
Structure and Mechanical Properties of Silk Fiber Stretched in Aqueous Solution of LiBr
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 33~38
The mechanical properties and structure of degummed silk fiber stretched after being treated with 8M LiBr aqueous solution were investigated. The mechanical properties of degummed silk fiber were depended upon the treatment temperature of 8M LiBr aqueous solution. That is, the strength and elongation had a tendancy to increase with the temperature, whereas the Young's modulus tended to decrease. The X-ray diffraction pattern showed that the crystalline orientation was disordered by 8
treatment neverthless destruction of the crystallites did not occur. rho silk fiber could be successfully stretched up 160% under the condition of 8M LiBr aqueous solution at 80 * after immersed for 1hr. For the highly stretched sample(160%), the tensile strength and Young's modulus were significantly improved in spite of lower elongation at break. According to the examination of X-ray diffraction pattern and SEM, the orientation tends to be occured more and more with stretching ratio. From the DSC thermograms, it shows that the thermal decomposition behavior was different between untreated and treated specimen with 8M LiBr aqueous solution. The maximun endothermic peak of thermal decomposition tends to increase to high temperature with the increase of stretching ratio.
Practical Performances of Methacrylamide-treated Silk Fibers
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 39~45
In this study the effects of methacrylamide-treatment on the physical properties and practical performances of silk were investigated for 38% MAA added silk fibers. The main results obtained were as follows; The values of tenacity, tearing strength, elongation, modulus and toughness decreased by the treatments of MAA on silk fibers. The moisture regain was increased by the MAA treatment, which improved the antistatic performance of silk fabrics. The stiffness values of MAA-treated silk fabrics increased and the performances of crease recovery were improved.
Construction of New Transfer Vector of Nuclear Polyhedrosis Virus of the Silkworm, Bombyx mori
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 46~51
In order to develope baculovirus expression vector system, we constructed new transfer vector of nuclear polyhedrosis virus of the silkworm, Bombyx mori. The promoter region containing only adenine of translation start codon of polyhedrin gene was cloned by polymerase chain reaction technique. And the 5' and 3' leader regions of polyhedrin gene was sequentially cloned. The polyhedrin coding gene was deleted from the ＋2 to the ＋597 position. As the result, we constructed new transfer vector which has EcoRI, SacI and KpnI sites for the cloning sites of foreign gene. New transfer vector was named as pBmKSKl. Escherichia coli
-galactosidase gene as foreign gene was inserted into pBmKSKl, under the control of the polyhedrin promoter and expressed in B. mori cells. The result showed that the new transfer vector pBmKSK1 is functional.
Effect of Hemolymph of Silkworm Larvae on the Multiplication of Bombyx mori Nuclear Polyhedrosis Virus in BmN-4 Cells
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 52~56
To investigate the effect of hemolymph of silkworm larvae on the multiplication of Bombyx mori nuclear polyhedrosis virus (BmNPV) in BmN-4 cells, BmN-4 cells were infected with BmNPV, which were sequentially Heated with the hemolymph exracted from B. mori larvae. When the culture media TC-100 containing 3% fetal bovine serum was mixed with 10% hemolymph heated at 65
for 30 minutes, the released polyhedra by multiplication of BmNPV in BmN-4 cells were increased more than those of non-treated. However, multiplication of BmNPV in BmN-4 cells treated with non-heated hemolymph was not effective, since non-heated hemolymph was toxic for the cell growth. The result of plaque assay showed that plaque forming units in BmN-4 cells treated with heated hemolymph are significantly increased, suggesting that efficiency of multiplication of BmNPV in BmN-4 cells is due to increase not of cell growth but of infectivity of BmNPV.
Distribution and Characterization of Bacillus thuringiensis Isolated from Soils of Sericultural Farms in Korea.
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 57~61
In order to isolate naturally occuring novel B. thuringensis strains, we identified the distribution of B. thuringiensis from soils of sericultural farms(spring and autumn season, respectively) of various regions in Korea. A total of 82 strains of B. thuringiensis producing spore and crystal were isolated. We examined oral toxicity against lepidopterous larvae, Bombyx mori, dipterous larvae, Culex pipiens and coleopterous, Sitophilus oryzae. The results showed that 82 B. thuringiensis isolates from sericultural farms were isolated and most isolates were toxic to lepidopterous larvae. Particularly, B. thuringiensis strains which is lepidopterous-active, lepidopterous-and dipterous-active were widely distributed. Also, non-toxic B. thuringiensis isolate was common.
Studies on the Toxicity of
-endotoxin of Bacillus thuringiensis to the Several Tissues of Hyphantria cunea Drury
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 62~67
Ultrastructural changes of tissues caused by Bacillus thuringiensis var. kurstaki
-endotoxin intoxication of Hyphantria cunea were observed by transmission electron microscopy. Bt
-endotoxin crystals induced the disruption of microvilli, vacuolation of cytoplasm, changes in the cisternae of the endoplasmic reticulum, disappearance of basal striations, loss of ribosomes, and changes in the configurations of mitochondria in the columnar cell of midgut. The fat body cells also showed spherical endoplasmic reticulum and distorted mitochondria, and then the cells were destroyed.
The Effect of
-endotoxin of Bacillus thuringiensis on the Protein Synthesis of the Several Tissues of Hyphantria cunea Drury
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 68~73
The toxic effect of Bacillus thuringiensis var, kurstaki
-endotoxin was determined in the midgut, fat body and Malpighian lobules from larvae of Hyphantria cunea using the au-toradiography and Western blot methods. Bt
-endotoxin crystals inhibited protein synthesis and elongation factor-2 activity of all tissues tested.
Some Ecological Characteristics of the Mulberry Scale, Pseudaulacaspis pentagona T., and Its Control with Insecticides
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 74~85
A study was conducted to provide some basic data for controlling the Mulberry scale insect(Pseudaulacaspis pentagona T.). Experiments were carried out with checking their field-life, and a few selected insecticides were tested when mulberry leaves were not in use. About 57% of female were survived from the overwintering attached to the bark. Over 80% of the scale were distributed within the range of 30cm from the base of each stem. One hundred percent and 96.7% of eggs were hatched under 25
respectively. Eighty percent of pupae emerged both under 25 and 27
. The Mulberry scale require about 33 days under 25
and 30 days under 27
. This species had 2 generations each year in mulberry field in Suwon. Overwintered as a mated female only. Oviposited about 170 eggs under their scale starting from the late April (in 1994) through the mid May (in 1993). Phenthoate EC sprayed in the mid June against the larvae was very effect with over 96% of control value.
Studies on Mulberry Shoot Rot caused by Fusarium spp.
Journal of Sericultural and Entomological Science, volume 37, issue 1, 1995, Pages 86~91
Isolation and pathogenicity of Fusarium spp. from mulberry shoot rot and severity of diseases which were known as bacterial blight were examined on four mulberry varieties in Suwon, Kongju and Chuncheon, A symptom of mulberry shoot rot was initiated long brown spot on young leaves and shoots. It was developed into dark brown spot and produced white mycelia and spores on the diseased symptoms. A symptom of bacterial blight showed leaf rolling and water soaking spot and produced bacterial ooze on leaf and shoot However later stage of upper two types of symptom was hardly distinguished. Severities of shoot rot and bacterial blight were 7.5% and 4.4% in Suwon, respectively. Isolation of Fusarium spp. on shoot rot symptoms was highter than that on bacterial blight symptoms, but isolation of Pseudomonas spp. was higher on bacterial blight symptoms. Trends of pathogenicity of Fusarium spp. and Pseudomonas spp. were similar to inoculation works, and isolations of pathogenic Fusarium spp. from center of symptom was higher than that from 30cm of symptom of all samples in three cultivation areas. Disease severities of shoot rot on variety of Kaeryangppong were 13.9%, 15.9% and 17.2% in Suwon, Kongju and Chuncheon, respectively. However variety of Cheongolppong was highly resistant to shoot rot disease in three cultivation areas.