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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 38, Issue 2 - Dec 1996
Volume 38, Issue 1 - Jun 1996
Selecting the target year
Effects of Mulberry Leaf on Physical Properties and Chemical Contents of Mulberry Leaf Noodle
Kim, Hyeon-Bok ; Yang, Seong-Yeol ; Lee, Yong-Gi ;
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 1~6
The physical properties of mulberry leaf noodle were compared with 3 level of rates(5, 10, and 20%) of mulberry leaf powder to wheat flour and 6 times of leaf picking seasons. The contents of rutin,
-aminobutyric acid (GABA) and inorganic compounds in mulberry leaf were analyzed with seasons and pretreatment of mulberry leaf. Dry matter content of mulberry leaf powder was about 20%. The leaf powder passed through 100 mesh sieve was 6% from fresh leaf weight. Through the taste test, 10% mulberry powder rate to flour was the best. Rutin content decreased with season, 319mg/100g DW on May 13, but 111mg/100g DW on May 26 and 43mg/100g DW on June 9. Rutin in dry leaf lost 37% by steaming and 83% by in boiling water. CAGA content on leaf decreased with season, 27.7mg/100g FW on May 13 and about 60% of on June 9.
Fundamental Study for RAPD-PR Analysis in the Silkworm, Bombyx mori
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 7~12
Reproducible the random amplified polymorphic DNAs(RAPDs) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200
M), MgCl2(1.5mM) and template DNA(30ng). In addition, anealing temperature ranging 35
by the adjusted condition was investigated and fixed at 35
in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorhpisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorhpims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDs detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori
Construction of the cDNA Library from Bombyx mori Larvae and Analysis of the Partial cDNA Sequences
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 13~18
To secure the genetic resources of silkworm, Bomyx mori, the cDNA library was constructed with mRNA isolated from fifth instar larvae. Titer of the cDNA library was about 1.3 X 106 plaques in total. We presumed that the titer covered all transcripts existed in Bombyx mori. Meanwhile, it is knowen that partial cDNA sequences, Expressed Sequence Tags(ESTs), have a good value for the discovery of novel genes and the elucidation of their structures. For this purpose, partial cDNA sequencing was carried out from randomly selected cDNA clones in the library. Partial cDNA sequences of 37 clones were determined and an average of 212 nucleotides of sequence can be read from the clone. The ESTs were searched in GenBAnk database and fifteen ESTs showed significant similarities to enlisted sequences. They included the genes of storage protein, heat shock protein, actin, catalase and so forth. We presumed that the 22 unmatched ESTs were novel genes.
Isolation and Characterization of Inducible Genes from Bombyx mori Injected with E. coli by Differential Screening
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 19~24
To investigate the genes which is related to immune reaction of Bombyx mori, differential screening was carried out using naive and induced B. mori mRNA probe. To begin with, we constructed the cDNA library with mRNA isolated from fifth instar larvae injected with E. coli(4 X 106 cells/larva) using Uni ZAP XR vector kit. Thirty-two inducible cDNAs showing higher intensity on the induced mRNA probing membranes were selected. Partial nucleotide sequences of 29 clones were determined and their expessed sequence tags (ESTs) were produced. Nineteen ESTs in 29 ESTs were matched in GenBank database and the rest of them were found to be unknown. These unmatched ESTs were presumed to be novel genes. The nineteen ESTs contained variable genes related to biological process in Bombyx mori and four classes immune genes. Four clones, BmInc 6, 8, 18 and 27 were similar to two antibacterial peptide genes, hemolin gene and transferrin gene, respectively.
Characterization of Chromium(VI) Adsorption onto Silk Fabrics
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 25~30
The adsorption mechanism of Chromium(VI) uptake in silk fibroin fibers was discussed. The adsorption equilbrium of Chromium(VI) is significantly influenced by the initial adsorption rate and it showed 52% of the equilibrium uptake. The Chromium(VI) uptake by silk fibroin in increased with the acidic range of pH, which react upon Chromium(VI) oxidations. The enthalpy change in the Chromium(VI) on the temperatures,
H, was found to be 39.7 KJ.mol-1, It means that the Chromium(VI) adsorption proceeds via a certain complex chemical reaction and the Chromium complex was found to be coordinated with carbonyl group of amides from the result of infrared spectra. The chroming of silk fibroin fibers in moderated in the conditions of 5
, pH 2.4, and 3 hours, which prevent from the loss of physical properties. The equilibrium adsorption is attained at 5 X 10-3M of Chromium(VI) solutions.
Development of Solid Culture Media for the Mass Production of Beauveria bassiana Conidia
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 31~35
To develope a solid culture media for the mass culture of Beauveria bassiana conida, wheat bran was selected as C source and N source. The pellet media were prepared without(P-I) and with(P-II) solidified ingredient. The moisture for the growth of B. bassiana was required over 4 : 2 of Media : D.W (W/V). The growth of Beauveria bassiana on the media type was more effective in media of pellet type than that of powder type. In addition, production of Beauveria bassiana conidia on the media size was more effective in media of S type(
3mm) than that of L type(
7mm). And the yield of Beauveria bassiana conidia at 2
cultivation on 3 weeks post inoculation was similar to that of
. Above-mentioned results showed that pellet medium (P-I) was effective to growth of Beauveria, suggesting that the pellet media may be useful in the mass production of Beauveria bassiana conidia.
Characterization of Recombinant Baculovirus Ewpressing Polyhedrin Gene of Bombyx mori and Autographa californica Nuclear Polyhedrois Virus
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 36~41
Recombinant baculoviruses having expanded host range were selected by coinfection of Autographa california NPV and Bombyx mori NPV into Sf-9 and BmN-4 insect cell lines. In order to determine the polyhedra morhplogy of RecS-A6, one of a recombinant baculovirus, polyhedra of RecS-A6 produced in insect cells were observed by phase contrast microscope and scanning electron microscope. The results revealed that the recombinant baculovirus had a various polyhedra morphology which was different from its parental viruses, suggesting that the various morhpology of recombinant baculovirus with an expanded host range was due to the genetic recombination of viral genome. To analyze the genomic recombinantion of the recombinant baculoviruses, genomic DNAs of two parent viruses and RecS-A6 were digested with restriction endonuclease and subjected to agarose gel electrophoresis. Southern blot analysis revealed that RecS-A6 has two polyhedrin gene of AcNPV and BmNPV in a viral genome. Polyhedral protein of recombinant baculovirus was analysed by SDS-PAGE. The result showed that molecular weight of polyhedral protein of RecS-A6 containing two polyhedrin gene of AcNPV and BmNPV was as the 31 kDa band of AcNPV and 30 kDa band of BmNPV.
Molecular Biological Characterization of Recombinant Baculovirus with an Expanded Host Range
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 42~47
To investigate the host range determining factors of nuclear polyhedrois virus (NPV), Autographa california NPV and Bombyx mori NPV were coinfected into the two different cell lines, BmN-4 and Sf-9. The recombinant baculoviruses, RecS-A6 and RecB-727 which have an expanded host range, were isolated from Sf-9 and BmN-4 cell lines, respectively. The molecular biological characteristics of the recombinant baculoviruses were investigated. The pathogenicity of RecB-727 was similar to that of wild type BmNPV, while the pathogenicity of RecS-A6 was relatively lower than that of wild type BmNPV. The restriction enzyme digestion patterns of parental viruses and recombinant viruses showed that the recombinant virus has an expanded host range by genetic recombination. Southern blot analysis revealed that the p10 gene of RecB-727 was derived from AcNPV genomic DNA, while RecS-A6 has p10 gene of BmNPV in a viral genome. To investigate the host range expansion mechanism of recombinant baculovirus, HindIII-SacI 0.6 kb DNA fragments of RecS-A6 and RecB-727 were cloned and sequenced. The results showed that of wild type BmNPV helicase gene, suggesting that the expanded host range of recombinant baculoviruses was due to the insertion of BmNPV helicase gene into AcNPV viral genome.
Maternal-mediated Transmission of Nosema bombycis Via the Surface and Internal Site of Silkworm Eggs
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 48~52
Nosema bombycis was found on the both of surface and inside of silkworm eggs layed by infected female moth, maternal-mediated transmission of those pathogens via the surface or internal site of silkworm eggs were investigated. All of the meconia from infected female moths contained pathogenic spores, those concentration were 4.6(
0.24)X106 /ml. The pathogens on the surface of nondiapause eggs were transmitted to the progeny larvae at the rate of 54.5%, however, lost their activity before hatching for the case of overwintered eggs or acid treatment(16% HCI at 46.2
for 6 min). N. bombycis in the silkworm eggs localized at the serosa, yolk, embryo except chorion tissue in the silkworm eggs layed by infected female moths. Development of the pathognes in the eggs was synchronized with embryogenesis, which secured the safety of pathogens against environmental condition, resulting in the high transmissibility of 91.2
The Molecular Biological Marker in Bombyx mori and Spodoptera frugiperda Cells
Jin, Byeong-Rae ; Je, Yeon-Ho ; Gang, Seok-Gwon ;
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 53~56
To investigate the molecular biological marker in insect cells, BmN-4 and Sf-0 cells were analysed by SDS-PAGE and random amplification of polymorphic DNA. The results showed that the patterns of total cell protein and random amplification of polymorphic DNA were distinguished between BmN-4 and Sf-9 cells, suggesting that the unique major bands were useful as molecular biological marker in BmN-4 and Sf-9 cells.
가잠유전자자원 및 유전형질
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 57~72
Journal of Sericultural and Entomological Science, volume 38, issue 1, 1996, Pages 73~74