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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 38, Issue 2 - Dec 1996
Volume 38, Issue 1 - Jun 1996
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Morphological and Biochemical Characteristics in the Korean Landraces of Mulberry Tree, Morus
Kim, Hyeon-Bok ; Kim, Nam-Su ; Park, Gwang-Jun ;
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 81~92
This study was conducted to characterize mulberry varieties by the analysis of morphological characters as well as biochemical and molecular biological markers. As for the budding stage Geumsang, Chosang i and Yeongbyeonchuwoo were early, but Dangsang 6, Hwangchuwoo were late. The lowest varietiy in rate of death atop was Dangsang 8(0.0%). Suncheonppong was the highest leaf yields in spring and autumn rearing season. In biochemical isozyme analysis, peroxidase gave good zymogram patterns in isoelectric focusing electrophoresis. There were high variations in RAPD analysis among the mulberry trees. From the obtained peroxidase and RAPD variations, cluster phylogenetic analysis was carried out using NT-SYS PC program. There were no clear grouping patterns between native varieties and leading varieties. The highest similarity was observed between Suwonsang 1 and Suwonsang 2 at about 90% similarity level.
Breeding of Artificial Autotetraploids from Cold Hardness Lines of Yongchonppong and Yeongbyonppong
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 93~99
By treatment(dropping) of 0.1~0.4% colchicine solution on the sprouts of winter buds of hard wood cutting slips for 4~5 days, two lines of artificial autotetraploids from Yongchonppong and one line from Yeongbyonppong were bred and the important cultivative characteristics of those new lines were as follows. The greentip sprouting stage of the new bred lines in spring season is later than the parental varieties by two days, but growth speed of the new lines after sprouting was faster than that of the parental varieties reaching the same level development with the parental varieties at the fifth leaf sprouting stage to be mid varieties same as the origins. The leaf shape of the new bred lines was wide round and the petioloes were long and thick. The thickness of leaf was thicker than the parental varieties by 17-33% and single leaf weight was heavy. The leaf area weight increased by 21-31% and the content of chlorophyll was also higher by 11-33%. With all the characteristics, the new breds produced good quality of leaves. The length and number of branches were shorter and less, respectively, than the parental varieties, but the internode length was either same or longer than the parents. Looking at the characteristics, the constitution of shoots was slightly inferior to the parental varieties. The cold hardness expressed by the death top rate of Sawonppong 23 and Sawonppong 24 was same level as that of Yongchonppong, but Sawonppong 25 was stronger than Yeongbyonppong in it with a high infection rate of dwarf disease. The productivity was lower than the parental varieties, but young shoot rate to shoot and branch and the ratio of leaf to young shoot were higher than the parental varieties. The fertility of Sawonppong 23 and Sawonppong 24 was comparatively high with 62% of cross success, but that of Sawonppong 25 was low with 23.9% of cross success.
A Study on the Antidiabetic Effect of Mulberry Fruits
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 100~107
This study was designed to evaluate the antidiabetic effect of mulberry fruits using insulin-dependent and/or non-insulin-dependent diabetes mellitus animal models. The administration of mulberry fruit did not affect either body wight or blood glucose level in the normal ICR mice and streptozotocin induced-type I diabetic mice group. In second experiment, prolonged mulberry fruits treatment did not significantly attenuate the blood glucose level in type I diabetes induced by streptozotocin. In third experiment, the antidiabetic effect of mulberry fruits have been investigated using type II diabetes animal model that was induced by administration of streptozotocin to 2-day-old rats. Significant decrease in blood glucose level was observed in prolonged mulberry fruits treated group. In these treated group, the weight of liver significantly decreased than that of control group. In fourth experiment using KK mice showing genetical type II diabetes mellitus, glucose tolerance has been significantly recovered in mulberry fruits treated group but not in control group. In conclusion, prolonged administration of mulberry fruits significantly reduced the blood glucose level in type II diabetic animals. However, the blood glucose level was not significantly reduced by prolonged mulberry treatment. These data suggest that mulberry fruits can be developed as functional food that has effect on the insulin-independent diabetus mellitus(type II daibetus mellitus).
Effect of Mulberry Leaf Sprayed with Rutin and GABA on Silkworm Growth
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 108~112
This experiment was carried out to investigate the feeding effect of rutin (quercetin-3-o-
-aminobutyric acid(GAB) sprayed on mulberry leaves on silkworm growth. Amount of ingest, digest and feces showed no difference statistically except rutin. Rutin made 6% increase of amount of digest, compared to the control. However, rutin or GABA decreased maximaly silkworm weight by 28% during the experimental period. GABA showed body weight loss by 15%, rutin by 7% respectively, compared to the control. Rutin and GABA extended three days larval period and extended one day mounting period and decreased 15.4% and 91% in number of mounted silkworm compared with the control, respectively.
Genetical Aspects of the Feeding Habit in Fb Strain, Bombyx mori.
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 113~117
To analyze the mode of inheritance of this gene in Fb strain, carried out cross experiment between Fb and several Japanese and Chinese origin strains. The results of crossing experiments, feeding ratio in F1 hybrids were nearly the mid of parents, F2 and backcrossed hybrids(BF1) were approximately intermediate between Fb and other strains. Hence, the feeding habit of cabbage leaves on Fb strain is assume to be incomplete dominance.
Effect of Folic Acid on Economic Traits and the Change of Some Metabolic Substances of the Silkworm, Bombyx mori L.
Nirwani, R.B. ; Kaliwal, B.B. ;
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 118~123
Dietary supplementation of folic acid to silkworm larvae in different concentration (100, 200 and 300
g/ml) to the 4th and the 5th instar resulted in a significant increase in economic parameters, such as female and male cocoon weights, shell weights, egg productivity and egg hatching percentage. However, larval duration was significantly decreased, as compared with that of carrier control. The increase in the glycogen and protein contents of the fat body was not significant, whereas the increase in trehalose content of haemolymph in all the treated groups and protein content of fat body and haemolymph in 100
g/ml treated groups were significant. However, the haemolymph protein was significantly decreased in 300
g/ml treated group.
Effect of Dimethyl Sulfoxide on Economic Traits and the Change of Some Metabolic Sustances of Bivoltine Silkworm, Bombyx Mori L.
Nirwani, R.B. ; Hugar, I.I. ; Kaliwal, B.B. ;
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 124~129
Oral application of dimethyl sulfoxide in different concentration (1, 3 and 5 percent) to silkworm, Bomyx mori at the fourth and the fifth instar has significantly increased the commercial characters such as larval and silkgland weights, female and male cocoon weights, their shell weights and egg production. However, larval duration, cocooning and hatching percentages did not showed any significant change, as compared with that of the corresponding parameters of the carrier control. Similarly the glycogen and protein contents of the fat body and trehalose and protein contents of the haemolymph were significantly increased in 1 and 3 percent, whereas the increase of them in 5 percent concentration did not take place significantly, except haemolymph trehalose where it was found to be significant, as compared with that of carrier control.
Influence of Thyroxine on the Economic Parameters of the Bivoltine Silkworm, Bombyx Mori L.
Hugar, I.I. ; Kaliwal, B.B. ;
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 130~137
Effect of topical application with 5, 10 and 15
g/ml thyroxine on the pre-cocoon, cocoon, postcocoon and silk improvement were studied in bivoltine silkworm NB18 breed of B. mori L. Thyroxine was applied independently on alternate days to silkworm of IV and V instar larvae. The treatment of all different concentrations resulted in a significant increase in commercial characters like larval weight, silkgland weight, cocooning percentage, female cocoon weight and its shell weight and male cocoon shell weight and its ratio, cocoon dimension, length of the filament and its weight and oviposition. However, there was no significant change in larvel weight in 5
g/ml treated group and larval duration was significantly decrease. But moth emergence percentage and hatching percentage did not show any significant change when compared with that of carrier control.
Effect of Tartaric Acid on Cooking of Tasar Silk Cocoon
Gulrajani, M.L. ; Das, Subrata ; Chattopadhyay, R. ; Sen, Kushal ;
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 138~143
Cooking of tasar silk cocoon of Deba variety (A. mylitta D.) with tartaric acid has been optimized by using an orthogonal central composite design. Shell weight loss and silk filament recovery are influenced by duration of treatment and acid concentration. Mechanical properties do not change with the process parameters. Hardness of water does not affect significantly the recovery of silk filament.
Characterization of Spodoptera exigua Nuclear Polyhedrosis Virus Polyhedrin Gene Structure
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 144~149
To develope the baculovirus expression vector system (BEVS) using Spodoptera exigua nuclear polyhedrosis virus (SeNPV), we characterized the polyhedrin of SeNPV. The SeNPV polyhedra was irregular and composed of the major protein molecular weight of 30 kDa determined by electronmicroscopy and SDS-AGE analysis, respectively. The nucleotid suquences of 876 bases including the coding region of polyhedrin gene was determined and it was revealed that the polyhedrin gene is located within Xho I 3.0Kb and Nco I 6.0 Kb by Southern blot analysis, respectively. Also, the Xho I 3.0 Kb and the Nco I 6.0 Kb fragments were cloned and restriction enzyme map of these clones were determined.
Growth Rate of Entomopathogenic Fungi in Mass Culture System
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 150~153
To develope a microbial pesticide for the control of agricultural and forestal pests in Korea, the mass culture system of entomopathogenic fungi was studied. Previously, we have developed the mass culture system which was adaptable for the culture of Beauveria bassiana. In this study, we determined the efficacy of this mass culture system for other entomopathogenic fungi, B. bassiana, Beauveria brongniartii, Metarhizium anisopliae, and Verticillium lecanni. To determine the efficacy of mass culture system, we examined the growth rate of entomopathogenic fungi in this system which was composed of 1st liquid media for growth of blastospore and 2nd pellet media for growth of conidia. As the result, we obtained that the blastopore numbers increased 103-104 times in liquid media at 72 hrs post inoculation. The results showed that this mass culture system for the growth of entomopathogenic fungi was effective.
Isolation and Characterization of Bacillus thuringiensis Toxic to Spodoptera Species in Kora
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 154~159
To isolate Bacillus thuringiensis toxic to Spodoptera species, we collected soil samples in Korea. In these samples, we characterized 7 B. thuringiensis isolates toxic to spodoptera exigua or S. litura from soil, granary and sericultural farm samples. The 7 isolates were named B. thuringiensis STB-1, STB-2, STB-3, STB-4, STB-5, STB-6 and STB-7, respectively. The bioassay of these isolates against S. exigua and S. litura showed highly insecticidal activity. The serotypes of them were determined by agglutination tests using 33 antisera ; STB-1 an STB-2 are identical to B. thuringiensis subsp. kurastaki, and STB-3, STB-4 and STB-5 are identical to subsp. kenyae. STB-6 and STB-7 did not react with 33 antisera. STB-1 and STB-3 which have different gene types from B. thuringiensis subsp. kurastaki and subsp. kenyae are identified new isolates. STB-6 and STB-7 which show no agglutination in serological tests havd cryIA(a), cryIA(b), cryIC, and cryII genes are also identified new isolates. Molecular weights of parasporal inclusions of all isolates were determined approximately 130 kDa by SDS-polyacrylamide gel elctrophoresis.
Changes in Properties of Silk Monofilament Caused by Drawing and Hydrolysis
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 160~167
The middle silk gland, that is a liquid silk thread gland consisting of silk protein, was taken out and a silk monofilament was made by drawing rapidly to approximately 3 times. In order to deteriorate the inter molecular hydrogen bonding force and to stretch in, the drawn silk filament was swoolen in boiling water. The results obtained are as follows ; The silk gland sample that just dried silk gland was occupied in crystalline region of silk-I type and random amorphous region. According to the examination of X-ray diffraction and thermal analysis, silk-II type crystal begins to appear partially in monofilament sample and spread to almost complet silk-II type crystal in 65.2% drawn sample. And, orientation of silk fibroin mlecule increased suddenly in early stage with a rise of drawing ratiofrom birefringence and density, and it was found that orientation of fibroin molecule was completed. As drawing ratio increases relation with time of hydrolysis, birefringence appeared almost fixed a tendency. Crystallization collapse by hydrolysis was not found in X-ray diffraction and thermal analysis. But, amorphous region began to flow by treated hydrolysis, that orientation of crystallization part was disturbed was supposed.
Dyeing on Silk with Vat Dye
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 168~174
Dyeing on silk with vat dyes which shows good color fastness at strong alkaline conditions were newly examined. The optimum conditions of dyeing bath to prevent the damage on the tensile strength and elongation were sodium hydroxide(NaOH) 10g/l, sodium hydrosulfite(Na2S2O4) 20~22 g/l, for 30 min at 5
. The color yield of silk fabrics was increase according to the concentration of NaOH and Na2S2O4, but decreased at higher concentration than that of NaOH 10g/l and Na2S2O4 22g/l. The vat dyeings showed excellent color fastness against washing and light, compared with the conventional dyeings.
Dyeing on Silk and Ramie Fabrics with Sappan Wood
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 175~179
The sappan wood dyeing conditions and mordanting methods suitable for ramie as well as silk were investigated. The HVC values of dyed silk ramie fabrics were varied by the amount of sappan wood in the dyeing bath, while the optimum weight of sappan wood for the extraction of dye was around 15g/l. The K/S value of dyed silk fabric showed no significant difference in the pH range of 3.5 to 6.5. However, the K/S values of dyed ramie fabric was increased proportion to the pH value of dye bath. The K/S value of dyed silk and ramie fabrics were most increased at the highest dyeing temperature in the range of 30 to 9
. By various mordantings, the HVC values of dyed silk and ramie fabrics were differentiated and it's color fastness could be improved.
Characterization of Fibroin Biosynthesis in the 5th Instar of Bombyx mori
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 180~185
Biosynthesis tracing of the silk fibroin in Bombyx mori silkworm was examined in vivo with isotopic [1-13C] Gly. labeling by nuclear magnetic resonance method. The [1-13C] Gly. labeled silk fibroin yielded very sharp 13C NMR signal in the posterior silk gland as well as in aqueous solution and the amound of [1-13C] Gly. labeled signal in the silkworm increased gradually and rapidly to 5-th day of fifth instar. However, the decomposition or decrease of the [1-13C] Gly. labeled signal occured from 5-th to 9-th day of fifth instar unexpectedly. These findings suggest that a relative amount of
-helical portion or amorphous silk II portion was formed without any further signal from 6-th day of fifth instar to pupation. Through peak separation of orientation spectrum, between the fiber axis and the molecular bond direction, N-H bond in Bombyx mori silk fiber as well as the orientation distribution around the silk fibroin axis were determined and two kinds of peaks were also obtained from this orientation spectrum.
곤충호르몬의 작용기작과 그 이용방안
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 186~188
뇌 및 휴면호르몬의 제어기구와 작용
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 189~196
유약호르몬(JH)의 작용기작과 그 이용
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 197~201
탈피호르몬의 작용기작과 이용
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 202~205
From Sericultural research to "Research on biological functions in insects"
Takeda, Satoshi ;
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 206~207
Journal of Sericultural and Entomological Science, volume 38, issue 2, 1996, Pages 208~209