Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 40, Issue 2 - Dec 1998
Volume 40, Issue 1 - Jun 1998
Selecting the target year
Ultrastructural Changes in the Cortical Cell Mulberry Trees(Morus)during Wintering Period
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 91~96
In relation to cold acclimation, this experiment was carried out to understand the changes of the cortical cells in the living barks of the mulberry during wintering period. The living barks of three mulberry varieties(Kaeryangppong, Shinilppong and Yongcheonppong) were sampled from December, 1995 to March, 1996. The result of this experiment was summarized as follows. The cortical cells in the living barks of the mulberry in December were filled with small vacuoles. Plastids and mitochondrias were located near the nucleus. At this time, almost all starch granules disappeared from the plastids. In January and February, mitochondria, palstids and microbodys of the cortical cell were observed. As increasing temperature from March, dictysomes and polysomes were sparse. Again, starch granules disappeared were observed in the plastids. From the above result. starch granules in plastide of the cortical cell of the mulberry disappeared during cold acclimation stage. After late January, Proplastid was observed in the cortical cell and the ultrastructures of cortical cell were actively changed.
Breeding of Bivoltine Silkworm Hybrids DP0308 and DP0314 in Sri Lanka : A Simplified but Practical Approach
Lea, Ho-Zoo ;
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 97~104
Mainly due to limited genetic resources available and also urgent need for hybrids suitable to local conditions, conventional theories and regular methods for bivoltine silkworm breeding are not easily applicable in such a tropical country as Sri Lanka which is recently planning to scale-up the silk industry. A simplified but practical methodology was introduced to overcome such constraints. Through application of such modified informal breeding methods, two hybrids named DP0308 and DP0314 were selected for cocoon production. Details of the altered method of germplasm improvement in the silkworm are presented, along with combining ability and heritability estimates for six parental pure lines in a half-diallel cross.
Effect of the Ecdysteroid UDP-Glucosyltransferase Gene of the Bombyx mori Nucleopolyhedrovirus on the Development of the Silkworm, Bombyx mori
;;Shizuo George Kamita;;
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 105~110
The baculovirus egt gene encodes an ecdysteroid UDP-glucosyltransferase(EGT) which catalyzes the transfer of glucose from UDP-glucose to the insect moltion hormone ecdysteroid resulting in a functionally inactive ecdysteroid. In baculovirus-infected insect larvae, EGT has been shown block molting and pupation. In this study, we compared the development of 4th and 5th instar silkworm, Bombyx mori, larvae injected with either wild-type bombyx mori nucleopolyhedrovirus (BmNPV) or a mutant BmNPV(BmEGTZ) in which the egt gene was disrupted by the insertion of a lacZ gene cassette. Larvae injected with BmEGTZ died roughly 12 h more rapidly compared to indentical larvae infected with BmNPV. In addition, BmEGTZ- infected larvae prematurely stopped feeding and gain less weight compared to BmNPV-infected larvae. In order to investigate why BmEGTZ-infected larvae died more rapidly than BmNPV-infected larvae, the array of hemolymph proteins in BmEGTZ-or BmNPV-infected larvae were analyzed by SDS-PAGE. The hemolymph of BmEGTZ-infected larvae showed virus-specific proteins, including polyhedrin, about 12 h earlier than the hemolymph of BmNPV-infected larvae
Construction of Stably Transformed Bm5 Cells by Using Autographa californica Nuclear Polyhedrosis Virus IE1 Gene
Cho, Eun-Sook ; Jin, Byung-Rae ; Sohn, Hung-Dae ; Chol, Kwang-Ho ; Kim, Soung-Ryul ; Kang, Seok-Woo ; Yun, Eun-Young ; Kim, Sang-Hyun ; Kim, Keun-Young ;
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 111~116
To construct transformed Bm5 cells, Autographa californica nuclear polyhedrosis virus (AcNPV)IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% uncleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/
G418 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistnt cells was isolated and characterized by PCR using AcNPV IE1 gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. moridrived Bm5 cells as well as Spodaptera frugiperda-derived Sf9 cells to produce stably-transformed insect cells.
Causal Pathogenesis on the Silkworm, Bombyx mori, Associated with Entomopathogenic Nematoda
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 117~125
Entomopathogenic nematodes, Heterorhabditidae and Steinernematidae, were isolated from the soil of mulberry field, and the high infectivity and invesiveness were confirmed in the silkworm, Bombyx mori. The cause of non-microbial and acute flacherie was found as an disease by infection with soil-born nematodes through the mulberry leaves contaminated with soil and rainwater. The causal nematodes were isolated by silkworm trap from all of the 5 soil samples collected on the 5 mulberry fields, and identified as 3 strains of Heterorhabditis sp. and 2 of Steinernema sp. Rainwater itself, however, wasn't engaged in the silkworm disease, mulberry leaves with rainwater was rather profitable for cocoon production when the leaf quality was too hard to feed silkworm. Feeding of wet mulberry leaves with rain might not so harm to silkworm when the condition of rearing room to be kept at suitable temperature and ventilated well. Nematode infection of silkworm could be occurred by harvesting and feeding of contaminated mulberry leaves on the weather condition of rainy and wind. For the prevention of nematode infection, silkworms should be fed the leaves harvested from the higher portion of the mulberry tree in rainy days. For an oppositional application of this susceptibility of silkworms to nematode, might be useful on the collection and amplification of nematode agents for biotic control of pest insects.
Comparison of Characteristics between Insecticidal and Noninsecticidal Basillus thuringiensis Strains belonging to Serotype H8a8b
Roh, Jong-Yul ; Je, Yeon-Ho ; Park, Hyun-Woo ; Chang, Jin-Hee ; Jin, Byung-Rae ; Lee, Dae-Weon ; Ziwen Yang ; Kang, Seok-Kwon ;
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 126~130
A noninsecticidal strain, Bacillus thuringiensis NTB-88, isolated from Korean soil, had a typical bipyramidal parasporal inclusion and its serotype is identical to B. thuringiensis subspmorrisoni (H8a8b). To elucidate differences between insecticidal and noninsecticidal strains, we compared strain NTB-88 to other toxic B. thuringiensis subsp. morrisoni strains (HD-12 and PG-14). Restriction endonucleases digested plasmid DNA patterns showed that strain NTB-88 was different from lepidopteran-toxic strain, HD-12, but it was similar to dipteran-toxic strain, PG-14. The gene type of strain NTB-88 was different from those of other insecticidal strains, Furthermore, the NH2-terminal amino acid sequence of crystal protein of strain NTB-88 had no relation to those of the previously known
-endotoxins in other toxic strains as well as HD-12 and PG-14 strains. Therefore, the noninsecticidal crystal protein in strain NTB-88 is novel and its property is different from insecticidal ones.
Changes of Silk Fibroin Molecular Weight by Plastein Reaction
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 131~135
Changes of silk fibroin molecular weight was studied by enzymatic proteolysis and reverse reaction of enzymatic proteolysis (plastein reaction) using chromatography, X-ray diffraction and thermal analysis methods. When the treatment of enzymatic proteolysis with
-chymotripsin to silk fibroin solution, a precipitate of Fcp fractions was formed. And, this was dissolved in LiBr aqueous solution, the precipitate of PIFcp fractions was obtained again. Fcp and PIFcp fractions showed silk IIand silk Itype structure, respectively. Fcp fractions was about 6,900 in molecular weight, PIFcp fractions obtained by plastein reaction on the precipitate of Fcp fractions increased molecular weight to abort 15,000. The molecular weight of Fcp fractions was increased by plastein reaction, but Fcp fractions almost transited to silk I type crystal. The structure of silk I type of PIFcp fractions was steady identified by X-ray diffraction and thermal analysis. As molecular weight of Fcp fractions was gradually low, PIFcp fractions was become to macromolecule little by little.
Effects of the Addition of Water-soluble Polymers on Silk Dyeing
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 136~142
The low-pollution dyeing condition and dyeing method suitable for the silk fiber were investigated. Instead of surfactants, water-soluble polymers and electrolytes were added as auxiliary agents in the new process of dyeing. By the new method, the level dyeing and sufficient exhaustion were achieved. The K/S values of silk fabrics were little different among electrolytes added in the dye-bath
The Characteristics of Silk Sericin/polyurethane Mixed Film
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 143~149
For the application of silk sericin, silk sericin powders were prepared by various spray dry conditions and the characteristics of silk sericin/polyurethane mixed films were investigated. When the sericin was dried from the solution at higher inlet and outlet temperature, larger sizes of the powder particles were obtained. It was also found that inlet and outlet temperatures were important factors affecting the shape and surface characteristics of sericin power particle. The many holes and empty spaces were observed at the surface and cross section of sericin/PU mixed film. With the increase of the amount of sericin powder in the mixed films, the size and number of holes and empty spaces were increased. The thickness of sericin/PU mixed film was increased with the content of sericin powder. As the portion of sericin powder increase, the tenacity is decreased while the elongation slightly increased.
Effect of Anti-Juvenile Hormone Analogue (AJH) Treatments on the Silk Filament Properties of the Silkworm, Bombyx mori L.
Moe ; Bae, Do-Gyu ; Sohn, Hae-Ryong ; Seol, Kwang-Youl ;
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 150~157
This study was carried out to define the effects of anti-juvenile hormone analogue(AJH) treatment at different silkworm larval stages on some silk filament properties. It was revealed that the treatments at the 1st and 2nd days of the 3rd instar as well as the 1st day of the 4th instar resulted to trimolters induction without lethal effect. The trimolters induced by treatment at the 1st day of the 4th instar showed the shortest larval duration and the lowest single cocoon weight, cocoon shell ratio, cocoon size, single filament length and denier of filament compared with those of the control, while the trimolters induced by the 3rd-instar treatment showed intermediate values. All treatments showed no significant differences from the aspect fo degumming ratio, elongation rate and crystallinity index of fibroin. However the silk filament obtained from the treatment at the 1st day of the 4th instar showed the highest tenacity which was revealed to be related to the better uniformity of filament by the means of microscopic photographing. FT-IR spectroscopy of the silk fibroin showed a sharp and strong peak at the position of 794.72 cm-1 in all AJH-treated samples regardless of trimolters induction, which was absent in control.
Structural Characteristics of Insect Chitin/Chistosan
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 158~162
The chitin was isolated from various kinds of insects such as exuvia of Psacothea hilaris Pascoe, silkworm pupa, Agrius convolvuli or from cuticle of cockroach by treatment with dilute HCI and NaOH. The chemical and crystalline structure was characterized by FT-IR and X-ray diffractometer. All of the chitins extracted from insects showed characteristic
-chitin peaks at the Bragg angle 2
, and 23.5
by X-ray diffraction analysis. The transition from chitin to chitosan was confirmed by IR spectra and the degree of deacetylation of the crab shell, silkworm pupa, cockroach, and Psacothea hilaris Pascoe was 70.9, 76.4, 75.5, and 74.1%, respectively. The double diffraction peaks of insect chitosan were observed at 2
, indication the characteristic of hydrated crystalline structure of chitosan.
Hydrolsis Behaviour of Antheraea pernyi Silk Fiber Treated with HCI
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 163~168
Hydrolysis rate and activation energy of Antheraea pernyi silk fiber treated with HCI were examined. Thermal decomposition temperature and surface morphology were also investigated by using differential scanning calorimeter and scanning electron microscope. As the concentration of hydrochloric acid and the treatment temperature increased, the hydrolysis occurred more rapidly. The activation energy of Antheraea pernyi, 74.0 kJ/mol, was higher than that of Bombyx mori, 58.1 kJ/mol. As the concentration of hydrochloric acid increases, the activation energy of Antheraea pernyi decreased from 74.0 kJ/mol to 62.0 kJ/mol. The shape of acid-resistance fraction of Antheraea pernyi became more distroyed and was transformed from fiber to powdered form with an increase of hydrolysis rate. The thermal decomposition temperature of Antheraea pernyi was 360.8
until the hydrolysis rate was 81.8 wt%, but ti decreased to 347.0
when the hydrolysis rate was 93.8 wt%
Morphology of Silk Fibroin/Poly(vinyl alcohol) Blend Film
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 169~175
The morphology of silk fibroin/poly(vinyl alcohol)(PVA)blend films was investigated using optical microscopy and confocal laser scanning microscopy. The effects of blend ratio and molecular weight of silk fibroin and PVA on phase separation were studied. Macro-phase separation occurred for the silk fibroin-rich/poor region whereas micro-phase separation took place for the dispersed/continuous phase, In spite of differences in molecular weight and blend ratio, it is observed that the dispersed phase and continuous one are composed of silk fibroin and PVA component, respectively. As the molecular weight of silk fibroin and silk fibroin content in blend ratio are decreased, the compatibility of blend is increased due to the reduction of micro-phase separation.
Production of Vegetable Wasp and Plant Worm by Injection of Paecilomyces japonica to Pupae in the Chinese Oak Silkworm, Antheraea pernyi
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 176~179
Injection method of Paecilomyces japonica to Antheraea pernyi pupal body was investigated on feasibility for production of the corresponding vegetable wasp and plant worm. Infection rate of P. japonica to the pupae was 100%, and also fruiting body formation rate was 97%. The initial pupal body weight of 9.38 g befor injection of P. japonica to the pupae was finally reduced to 7.87g after two weeks in vivo culture. From these results, the injection method to pupal body will be useful for the production of Antheraea vegetable wasp and plant worm in future.
뽕나무 관련연구의 최근동향과 발전방향
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 180~184
곤충기능이용기술개발에 의한 21세기 곤충생물산업화 연구개발 전망
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 185~190
병리분야의 학술동향에 관한 사상적 배경과 발전 방안
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 191~202
견피브로인을 이용한 최근 소재개발 동향
Journal of Sericultural and Entomological Science, volume 40, issue 2, 1998, Pages 203~212