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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 41, Issue 3 - Dec 1999
Volume 41, Issue S2 - Oct 1999
Volume 41, Issue 2 - Sep 1999
Volume 41, Issue 1 - Jun 1999
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Electrophoretic Patterns of Hemolymph Proteins of Varieties with Long and Short Life Span in the Silkworm Bombyx mori L.
Kang, Pil-Don ; Yoon, Hyung-Joo ; Ryu, Kang-Sun ; Sohn, Bong-Hee ; Sohn, Hung-Dae ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 1~8
Hemolymph protein patterns of silkworms in terms of short and long life span were analyzed by native- and SDS-polyacrylamide gel electrophoresis (PAGE) with the developmental stages. From the native-PAGE patterns of silkworm major hemolymph proteins there were varietal differences on the first day of the pupal stage and were classified into there groups MHP-a, b and c SA 10, JAM109 and J037 were grouped into MHP-Ⅰ, Hangang, Chungmun and Daizo(sdi) into MHP-Ⅱ and NTZN, Sulak, Qoichuk and PR varieties into MHP-Ⅲ group. It was found that the MHP in each group revealed similar patterns and changes with development of pupal stage. In the first day of adult, MHP-c was clearly detected in both female and male of the Daizo(sdi), but not in the J037, indicating that there was significantly varietal differences in electrophoretical protein pattern. In addition, the results of protein pattern of hemolymph by SDS-PAGE showed also varietal differences in the concentration of hemolymph protein.
Pharmacodynamic Study of Silkworm Powder in Mice Administered to Maltose, Sucrose and Lactose
Ryu, Kang-Sun ; Lee, Heui-Sam ; Kim, Sun-Yeou ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 9~13
The experiment was carried out to investigate the absorption profile of blood glucose in mice administered to silkworm powder MeOH extract. The mice was injected to oral load of maltose, sucrose and lactose(2 g/kg) and silkworm powder MeOH extract at the same time. After injecting the sample, blood glucose concentration was measured at 0, 30, 60, 120, 180 and 240 minutes. Blood glucose lowering effect of silkworm powder for loading maltose was 69% in postprandial 30 minutes. After the postprandial 60 minutes, the glucose was absorbed slowly. Total amount of blood glucose absorption in mice administered to maltose were 560.7 mg/dl during 240 minutes. That of silkworm powder MeOH extract marked 534.7 mg/dl. Total amount of blood glucose from oral loading sucrose reached to 508.9 mg/dl. That of loading silkworm powder MeOH extract were 468.8. But, silkworm powder was not inhibited lactose absorption. As a above results, silkworm powder inhibits the transient rising of blood glucose after postprandial 30 minuts through inhibition of
-glucosidases. In case of starvation silkworm powder don't promote the hypoglycemia. In addition, silkworm powder induces the delay absorption of glucose without loss of it.
Utilization of Grass Powder as an Ingredient of Artificial Diet fro Silkworm, Bombyx mori L.
Seol, Kwang-Youl ; Hong, Seong-Jin ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 14~19
The cost of artificial diets for silkworm is very expensive, so that it is essential to lower it in order to extend the period of silkworm rearing on artificial diets. In the present paper, the utilization of several kinds of grass powders was tried to use as an ingredient of artificial diet to reduce its production cost. The value of grass powder as an ingredient of artificial diet was assessed on the base of ingestability and growth of silkworms. As a result it was found it was found that they varied with the kinds and harvesting season of grasses. But it is appeared to be possible to use a grass powder as an ingredient for silkworm diet, when it contains 10~20% of grasses. Moreover, to cut the cost of diet, it is recommanded that the diet containing grass powder could be a applied to the polyphagous silkworm variety.
Genetic Expression of Color Approved by Color Qualities of Munsell System on the Cocoon of Silkworm, Bombyx mori
Han, Myung-Sae ; Park, Hyun-Ro ; Han, Sang-Mi ; Nam, Ki-Soo ; Kwon, Soon-Ha ; Lim, Jong-Sung ; Nguyen, Mau Tuan ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 20~28
Color qualities investigated on the basis of Munsell code and Korean standard color code for the cocoons from various strain of mulberry silkworm, Bombyx mori. 16 of different color qualities were identified from 21 of original silworm strains, and determinated international name with the revision of Korean color name for cocoon. The various cocoon color confirmed on the sphere from 567 to 593 nm wavelength, 78% of those located at the region about 580 nm (575~584) of sensitive "color difference limen". Y gene engaged broad ranges of wavelength (575~593 nm) in the color expression, by contrast with other genes of Pk (593 nm), F (584~593), Grc and relative G group (567~570 nm), on the transmission of carotenoid or flavonoid color substance. YC gene expression by original silk worm strain was also distinguished by those variation of specific yellow than other colors from Grc, GaGb, Gc, and YPkF. Appearance of chrome yellow cocoon was dominant than other yellow in the cross among vivid yellow group. F1 of pin
green produced the cocoon of yellow such as "additive mixture" as color light, however, most of the hybrid between yellow cocoon showed the color similar to "subtractive mixture" as a mixture of dyestuff. Hybrid cocoons among yellow or green colors were decreased their hue, value, and chroma, than those of parent. Diallel cross among the strain of various green cocoon suggest the existence of Grc, Ga, Gb, Gc genes. Cream colored cocoon of B. mandarina was differed from other yellow cocoon of Bobyx mori B. mori. Y
with Ymc showed the similar role of Y with C, therefore, segregated yellow cocoon from the B. mori
B. mandarina (+
). YC expression of
mc genes might be suppressed by deficiency of outer layer sericin on the middle division of silk giand in the B. mandarina.
Effects of Silkworm Powder, Mulberry Leaves and Mulberry Root Bark Adminstered to Rat on Gastrointestinal Function
Lee, Heui-Sam ; Kim, Sun-Yeou ; Lee, Yong-Ki ; Lee, Won-Chu ; Lee, Sang-Duck ; Moon, Jae-Yu ; Ryu, Kang-Sun ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 29~35
This experiment was carried out to investigate the effect of silkworm powder, mulberry leaves and mulberry root bark administered to S.D. rat on gastrointestinal function. Plot containing ten percent sericultural products(silkworm powder, mulberry leaves and mulberry root bark) was supplied with S.D. rat. The food efficiency ratio(FER) of S.D. rat fed with sericultural products were 0.1486~0.1573. The transit time also were 512.5~574.0 minutes, and transit speed were 25.05~29.11 mm/min. The pH of S.D. rat's feces fed with sericultural products were 5.95~6.72. The daily amount of S.D. rat's feces were 4.12~5.42g. As above results, sericultural products was evaluated to improve the function of S.D. rat's gastrointestine.
Cultural Condition and Morphological Charateristics of Paecilomyces Japonica for the Artificial Cultivation
Nam, Sung-Hee ; Jung, I-Yeon ; Ji, Sang-Duk ; Cho, Sae-Yun ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 36~40
The optimal condition for the production of the artificial fruiting bodies of Paecilomyces japonica in bulk was determined from the observation of the conidial, hyphal, and cultural condition and the medium. The colony was grown 32 mm diameter in 14 days on potato dextrose agar(PDA). Conidia was irregularly long oval-shaped and measured 4.07
m in average. The hyaline hyphae formed transparent bundles which branched out. The fruiting body on the insect surface was measured 30 to 50 mm in length and formed up to 55 branches. Potato dextrose(PD) both was the most effective for the growth of Paecilomyces japonica among the liquid type media. About 3.1 mg mrcelia in dry weight were produced in 50 ml/PD broth. The best condition for the production of conidia under which condition 4.3
conidia/ml were harvested was pH 5.0 in acidity and 20
following preculture at 24
in temperature for 7 days.
Structural Charateristics of Silk Fibroin Gel on The Preparation Conditions
Lee, Kwang-Gill ; Lee, Young-Woo ; Yeo, Joo-Hong ; Nam, Jin ; Kweon, Hae-Young ; Park, Young-Hwan ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 41~47
Silk fibroin dissolved in highly concentrated calcium chloride and ethanol mixture aqueous solution turned into gel under suitable conditions. Preparation conditions and properties of gel were investigated as a function of parameters such as pH of solution, fibroin concentration, glycerol concentration and molecular weight. When pH of silk fibroin aqueous solution was near the isoelectronic point(pH 3.9~4.0), gelation occurred rapidly and strength of gel was stonger than that of pH-unadjusted due to electrostatic repulsion decrease between silk fibroin macromolecules. As concentration of silk fibroin and glycerol was higher, gelation occurred more rapid. FT Infra-red spectra of freeze-dried fibroin gel showed that gelation was derived by intermolecular anti-parallel
-sheet structure formation. In addition to, it was found that white-precipitate occurred instead of gelation when aqueous silk fibroin was treated by enzyme(flavouzyme), however, after flavouzyme-treated silk fibroin aqueous solution was centrifugated gelation occurred instantly. The results of differential scanning thermal analysis and infra-red spectroscopy showed that thermal stability and crystallinity of enzyme-hydrolyzed fibroin are superior to those of unhydrolyzed fibroin.
Acid Hydrolysis of Insoluble Sericin
Kim, Jeong-Ho ; Bae, Do-Gyu ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 48~53
The study was carried out to investigate the effects of hydrolysis conditions such as treatment times and concentrations on the solubility of insoluble sericin using the hydrochloric acid solution. When insoluble sericin was hydrolyzed by HCl solution, the solubility was increased with the higher treatment concentration. As the results of electrophoresis of sericin powder obtained by the HCl treatment, a distinguishable band was not confirmed. Average degree of polymerizations(A.D.P.) of sericin hydrolyzed by HCl solution were about 4.2~5.9 and average molecular weights(M.W.) were about 470~670. The longer hydrolysis time reduced the whiteness of sericin powder. As the results of amino acid analysis, the amino acid compositions of the sericin powder from HCl treatment were sililar to that of insoluble sericin, but Tyr. and Arg. were not detected in the powder obtained by HCl treatment. In DSC analysis, thermal deformation and pyrolysis peak located at near 220
Characterization of Tussah (Antheraea pernyi) Silk Fibroin Powder Prepared by HCI and NaOH
Kweon, Hae-Yong ; Lee, Kwang-Gill ; Lee, Yong-Woo ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 54~60
Antheraea pernyi silk powder was prepared by treatment with HCl and NaOH. The degree of hydrolysis of Antheraea pernyi silk fiber was examined. The morphology and structural characteristics of Antheraea pernyi silk powder were investigated by using SEM, FTIR and X-ray diffractometer. As the concentration of HCl and NaOH and tratment temperature increased, in general, the degree of hydrolysis of Antheraea pernyi silk fiber increased. On the other hand, the degree of hydrolysis of Antheraea pernyi treated with 3 N NaOH at 120
for 24 hr was 70 wt%, which was lower than that of 90
(83 wt%). The morphology of acid/alkali resistance fraction of Antheraea pernyi silk fibroin was transformed from fiber form to powered one with an increase of hydrolysis. The conformation of Antheraea pernyi silk powder characterized by FT-IR spectrometer and X-ray diffractometer
Dyeing of Treditional Fabrics with Natural Dyeing
Chung, In-Mo ; Lee, Yong-Woo ; Woo, Soon-Ok ;
Journal of Sericultural and Entomological Science, volume 41, issue 1, 1999, Pages 61~69
The dyeing experiments were done by using tannin pigments and herbs for natural fabrics such as ramie, hemp and silk. The anti-bacterial ability and color fastness were examined for dyed fabrics. Dye materials containing tannin pigment such as oak tree leaf showed rich dyeing on ramie, hemp, and silk fabrics when the pH of dye solution is lower as well as the dyeing temperature is higher. In crimson dyeing, the colour of fabric showed red-purple when the crimson pigment was extracted at 40
, while yellow-red when extracted above 70
. The colour fastness of dye materials containing tannin pigment was excellent showing 3~4 grade or above, while most of those derived from chinese medicine showed poor colour fastness of below 3 grade. The anti-bacterial ability of dye materials derived from chinese medicine was high, since the sappan wood fabric showed low bacteria reduction rate.