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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 42, Issue 2 - Dec 2000
Volume 42, Issue 1 - Jun 2000
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Fertility and Some Agronomic Characteristics of ′Sawonppong26′ Autotetraploid Artificially derived from Keomseolppong (Morus spp)
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 67~72
Autotetraploid 'Sawonppong 26'was induced by dropping clochicine solution in growing point of Keomseolppong which is of high rootability and some agronomic charactelistics are as follows. Sawonppong 26 is a tetraploid of Keomseolppong with 56 of the chromosome number. Its fertlization percent was 59.4% and the germination percent of sank seed was normal. Sugar degree of mulberry fruit was 13.3% and total acidity was 0.69%. Single fruit weight of Sawonppong 26 was increased by 79%, as compared to that of Keomseolppong. Even though percent of survival of hard wood cutting was 94%, number of rootings was less and diameter of root was slender, it is expectedly a promising variety to be used for a breeding parent from the aspect of rootability.
Sensory Characteristics of Mulberry Fruit Jam & Wine
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 73~77
Sencory characteristics of mulberry fruit jam and wine were investigated by according to mulberry fruit varieties, including Daeryukppong, Kuksang 20, Chongilppong and a wild mulberry variety. Palatability ranks of mulberry fruit jam by according to mulberry fruit varieties were Kuksang 20(34.5%), Chongilppong(32.8%), Daeryukppong(17.2%) and Wild variety(15.5%). In the sensory characteristics, we obtained 6.32 points as total mean point. In case of mulberry fruit wine, panels were favorits the Chongilppong best. But, in the sensory characteristics, we obtained low points(=5.47) as total mean point. From these results, it seems that alcohol content was very important factor. We selectd two desirable mulberry fruit varieties(=Chongilppong and Kuksang 20) for manufacturing materials.
Removal of Chlorine from Aqueous Solutions by Mulberry Leaf Powder
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 78~82
In this study, a comparative removal of chlorine from aqueous solutions of mulberry leaf powder(MLP) and activated carbon(AC) was investigated. The chlorine removal capacities of MLP and AC were shown as a function of contact time, pH and initial chlorine concentration. Optimum contact time and removal pH value of MLP were determined as 2 hr and pH 10, respectively. Chlorine removal increased with increasing initial chlorine concentration up to 1.3g/L. Both Langmuir and Freundlich adsorption models were suitable for describing the short-term removal of chlorine by MLP and AC. According to Freundlich adsorption isotherms, the maximum removal capacity of MLP(0.264 mg Cl
/mg) was nearly two times greater than that of AC(0.56 mg Cl
/mg). These results suggested that MLP might potentially be used as an alternative to traditional water treatment materials for removal of residual chlorine in drinking water or process wastewater.
Hair growth effects of Mori Cortex Radicis Mixture on the hair of rat
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 83~85
This study was carried out to investigate the hair growth effect of Mori cortex radicis mixture on the hair of rat. Cytarabine(50mg/kg) was injected to eight-day-old rats for 7 days. When the mixture of Mori cortex radicis was administered to the rat by route of skin, it promoted the growth of hair. These data suggest that Mori cortex radicis mixture have an effect on the hair growth in cytarabine-induced alopecia rat.
Phenotypic Characteristics of New Mutant, Non-molting f(nm-f) of Bombyx mori
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 86~92
Studies were carried out to investigate the phenotypic characteristics of the non-molting mutant (nm-f) which was mapped on the 2nd linkage group. The results obtained were as follows : The nm-f mutant was distinguishable in the 3rd day of hatching. About 80 percentage of the non-molting mutant larvae died at the first instar within 10 days of hatching. The remaining larvae survived to the 2nd ad the 3rd instar but did not live to the final instar. There was no difference in non-molting nutant manifestation between hibernating and artificial hatching eggs. As a result of hemolymph protein analysis, the protein content on nm-f mutant was less than the normal larvae's. Therefore, we conclude that the characterization of nm-f is similar to the already known strains of non-molting mutant and the shortage of hemolymph protein is closely related to the non-molting characteristic in nm-f.
Effect of Silkworm Powder on Oxygen radicals and Their Scavenger Enzymes in Brain membranes of SD Rats
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 93~98
This study was designed to investigate the effect of silkworm powder on oxygen radicals and their scavenger enzymes in brain membrances of SD rats. Hydroxyl radical (OH) levels resulted in a considerable decreases in brain mitochondria fraction. Superoxide radical (O
) levels were a slightly decreased in brain cytosol fraction. Lipid peroxide (LPO) and Oxidized protein (OP) levels were significantly decreased in brain mitochondria and microsomes fraction. Mn-superoxide dismutase (SOD) activity was remarkably increased in the mitochondria fraction. Cu and Zn-SOD activities were effectively increased in brain cytosol fraction. GSHPx activity was considerably increased in brain cytosol fraction. These results suggest that anti-aging effect of silkworm plays an effective role in attenuating an oxidative stress and increasing a scravenger enzyme activity in brain membranes.
Characteristics and pathogenicity of the Entomopathogenic Fungus Beauveria bassiana101AA on the silkworm (Bombyx mori)
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 99~103
This study was carried out to investigate incubating characteristics, LTSEM observation, nature of a disease of infected silkworms(bombyx mori), LD
50/, optimum infective condition for mass production and infection percentage(%) on conservation periods. On the V8 media, the colony was 0.5∼2.0cm/8 days in diameter and white or slighty colored with a white fluffy to powdery appearance. The conidia was subglobos in shape, zig-zag appearance, 1-celled, hyaline and 3.2
2.4 ㎛ in size on the average. The conidiophore was irregular grouped, hyaline, rounded or flask-shaped. The LD
50/ values of the 2nd and 4th silkworms were each other 6.6142(Log) in natural temperature and humidity (25
, 65%). On optimum infective condition for mass production, all of preservation time(hr.) postinoculation in 1.0
8/ conidia/㎖ was over 97% and only 20 hr in 1.0
7/ condia/㎖ was over 90%.
Enzyme Hydrolysis of Insoluble sericin
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 104~108
To hydrolyze insolule sericin the enzyme hydrolysis was used, and then obtained the results as given belows. When insoluble sericin was hydrolyzed by enzyme treatment, the solubility was best at pH 7, 60
and was slightly increased both above 2 hours treatment and above 10% of enzyme concentration. As the results of electrophoresis, the distribution of molecular weight of sericin powder obtained by enzyme hydrolysis was very weak and showed in the wide range having no distinguishable band. Average degree of polymerzations (A.D.P.) of sericin hydrolyzed by enzyme were about 4.1∼6.3, average molecular weight were about 470∼730. The whiteness of the sericin powder obtained by enzyme hydrolysis was high and increased slightly with higher treatment concentration of enzyme. As the results of amino acid analysis, the amino acid analysis, the amino acid composition of the sericin powder from the enzyme treatment were similar to which located at near 230
. The peak of near 230
could not been found in the sericin powder obtained by enzyme hydrolysis.
Preparation of Multi Skin Care Gauze by Blending of Silk Fiber and Separated Cellulose from Waste Milk Pack
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 109~113
The preparation of skin care gauze could be make to mixing separated cellulose from waste milk pack and degummed silk fibroin fiber. Also, its wound covering and anti-bacterial activity were investigated in order to find out the enhancement of their functionality. By the 30% silk fibroin fiber including skin care gauze, the anti-bacterial activity values of Staphylococcus strain are much 4 times higher than of 0∼10% silk fibroin fiber including skin care gauze. The average yield of cellulose from waste milk pack was obtained 50-60%, and their morphologies, physical properties, modulus and biodegradation ratios are studies, respectively.
Structural Characteristics of Regenerated Antheraea pernyi Silk Fibroin Film treated with ethanol
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 114~119
Effects of ethanlo treatment on the structural and thermal characteristics of regenerated Antheraea pernyi silk fibroin (RSF) were investigated. Infrared spectroscopy and X-ray diffractometry showed that the conformational transition of RSF might be affected by concentration of ethanol and its treatment time. The structure of RSF was rapidly changed from random coil to
-sheet conformation when RSF was treated with les than 75% ethanol concentration. However, RSF treated with ethanol(100%) did not show conformational change. Differential scanning calorimetry showed that exotherm at 232
disappeared and the intensity of endotherm at 228
decreased with treatment of 75% ethanol. Dynamic thermal analysis showed that loss modulus (E") and tan
E/ of RSF treated with aqueous ethanol was broaden and shifted to higher temperature in comparison with those of untreated RSF.
Effects of Silk Fibroin Powder on Lipofuscin, Acetylcholine and Its Related Enzyme Activities in Brain of SD Rats
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 120~125
This study was designed to investigate the effects of silk fibroin (Mw 500) powder (SFP) on lipofuscin, acetylcholine (ACh) and its related enzyme activities in brain of rats. Sprague-Dawley (SD) male rats (160
10 g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFp-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. In case of liver membranes, lipofuscin (LF) levels resulted in a considerable decreases (11.5% and 13.8%, respectively) in SFP-2.5 and SFP-5.0 groups compared with control group. But in case of brain as the most sensitive organ, LF levels were remarkably inhibited about 18.3% and 21.7% in SFP-2.5 and SFP-5.0 groups compared with control group. Acetylcholine (ACh) levels were considerable decrease (3.0% and 9.2%, respectively) in brain membranes of SFP-2.5 and SFP-5.0 groups compared with control group. choine acetyltranferase (ChAT) activities as a synthesis enzyme of ACh, and acetylcholinesterase (AChE) activities as a hydrolysis enzyme resulted in a slight increases (2.4% and 3.0%, 4.6% and 6.3%, respectively), but significance difference between ChAT and AChE activities by SFP administration could be not obtained. Monoamine oxidase-B (MAO-B) activities were significantly inhibited (9.5% and 12.6%, respectively) in brain of SEP-2.5 and SFP-5.0 groups compared with control group. These results suggest that inhibiting effects of LF accumulation and MAO-B activity of silk fibroin(SFP) may play a pivotal role in protecting learning memory impairments by attenuating a various age-related changes for improvement of brain function.
Trend and Technology of Gene and Genome Research
Journal of Sericultural and Entomological Science, volume 42, issue 2, 2000, Pages 126~141
A major step towards understanding of the genetic basis of an organism is the complete sequence determination of all genes in target genome. The nucleotide sequence encoded in the genome contains the information that specifies the amino acid sequence of every protein and functional RNA molecule. In principle, it will be possible to identify every protein resposible for the structure and function of the body of the target organism. The pattern of expression in different cell types will specify where and when each protein is used. The amino acid sequence of the proteins encoded by each gene will be derived from the conceptional translation of the nucleotide sequence. Comparison of these sequences with those of known proteins, whose sequences are sorted in database, will suggest an approximate function for many proteins. This mini review describes the development of new sequencing methods and the optimization of sequencing strategies for whole genome, various cDNA and genomic analysis.