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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 43, Issue 2 - Dec 2001
Volume 43, Issue 1 - Jun 2001
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Phylogenetic Relationships and Characterization of Korean Native Silkworm Strains Based on RAPDs and Isozyme Analysis, Bombyx mori
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 59~66
This study was conducted to elucidate phylogenetic relationships and genetic characterization of silkworms that might be recognized as the Korean native strains. Genetic characterization in isozymes and the proteins of larval hemolymph of 17 silkworms were observed by acrylamide gel eletrophoresis, on 12 genes; Bph, Bes, les, Amy-hc, Ict-A, -B, -D,-E,-H, Pfl, Pst, Lp. Gene frequencies in each locus were compared other geographic strains. Korean native strains were remarkably different from others considered as the genetic characterization of Korean native strains. Phylogenetic relationships in Korean native strains were analysed using RAPD-PCR markers. A total of 40 primers were used and 346 bands of amplified DNA were generated from geographic strains. Genetic similarity based on the RAPD bands was used to construct phylogenetic dendrogram based on analysis of bard sharing data of amplified markers. Genetic similarity ranged from 0.595 to 0.860. In the genetic relationship based on dendrogram, they were classified into Bombyx mori group (including 16 domesticated silkworm strains) and B. mandarina group. The Bombyx mori group was separated into three sub-groups at the genetic similarity of 0.6930, including Korean, Japanese and Chinese groups. According to this result, the Korean native variety can be considered as a clearly different variety from other geographic strains. It may be concluded that the Korean native strains are also one of original geographic variety such as Japanese, Chinese, etc.
Genetic analysis and characteristics in the crescent-egg mutant, cre, of Bombyx mori.
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 67~76
The "crescent-egg" a new spontaneous mutant was detected in a white egg strain k37. Studies were carried out the linkage analysis, to investigate phenotypic characteristics and biochemical analysis of haemolymph and ovarian protein. The mutant, ore was independent from 20 linkage groups P(2), Ze(3),L(4), oc(5), sn8), Ia(9), w-1(10), K(11), ch(13), U(14), bl(15), cts(16), bts(17), mln(18), nb(19), oh(20),Lan(21), or(22), tub(23) and Xan(27). The fertilization, hatchability and larval growth were not different from the those of normal eggs. The content and composition of yolk protein were similared to normal eggs. Scanning electron microscopy revealed the areal specific structure in dorsal region of egg-shell of cre mutant. Analysis of chorion protein by isoelectrofocusing(IEF), was resolved no difference in the composite of the chorion protein. We conclude that the egg mutant ere is expressed only in the egg-shape formation and region specific determination.
Effect of Diflubenzuron on Silkgland Development of Silkworm, Bombyx mori (Lepidoptera: Bombycidae)
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 77~82
This study was investigated to know the effects of diflubenzuron(DFB) on the larval silkgland development of the silk-worm, Bombyx mori (L.). It has been known as a prohibitor of chitin synthesis mostly on the species of the Order Lepidoptera. In this work, the effects of the DFB concentrations (2.5
-2/, and 2.5
l on the various larval stages, were investigated in terms on the silkgland. The macro- and microstructure of cell membrane of silkgland, and the differences of silkgland weight and water contents treated by DFB are also surveyed. As the tesults, the silkgland weight depended sensitively on not the DFB concentration but the DFB treatment period. The longer DFB treatment period, the lighter dried silkgland weight and the heavier water content of the silkgland. White opaque(WO) emerged in the middle silkgland of DFB treated larva. From the scanning election microscope observation, the cell membrane of silkgland of DFB treated larva was distinctly more compact compared to that of control. The WO was evidently resulted from the obstacles of normal transformation of silk protein through the cell membrane of middle silkgland.
The Medium Development for Entomopathogenic Fungi by Using Silkworm Powder
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 83~87
This study was carried out to investigate the optimal medium and optimal dextrose concentration of 12 entomopathogenic fungi. The colonies of Cordyceps gracilioides, C. sp.(J23), Paecilomyces farinosus(J5), P tenuipes, Paecilomyces sp.(J4), Beauveria bassiana and B. brongniartii on SLA(silkworm larva agar) medium are similar to those on PDA(Potato dextrose agar) medium. On the contrary, the colonies of 5 fungi are very various according to its medium. The optimal medium for 1 1 fungi was SLA-A medium, which was better than PDA medium with regard to the mycelial growth. The optimal concentration on mycelial growth was superior to 6% for 6 fungi. When silkworm larvae was injected with P. tenuipes on SLA medium for fruiting body production. Infection rate, fruiting body formation rate, synnemata number and synnemata length were 91.3
3.2 and 33.4
2.88 ㎜ respectively. In economical analysis, the prototype medium can reduce 61.9% of production cost per 1 liter compared with PDA medium.
Interspecific crossabilities among wild mulberry species and cultivars growing in the Korean Peninsula
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 88~92
The crossabilities among 3 wild mulberry species and 3 cultivars growing in the Korean Peninsula had been studied. The result of the crossabilities of Moms tiliaefolia with Moms bombycis and 3 cultivars were possible, but with Morus mongolica was impossible. Those of Moms mongolica with Moms bombycis, Morus tiliaefolia and 2 cultivars except Daeryukppong were possible too. And those of Morus bombycis with Moms titiaefolia, Moms mongolica were impossible, but with 3 cultivars were possible moderately.
Fertility and Characteristics of Mulberry fruits on Daejappong and Garmbacppong (Morus Spp.)
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 93~98
Major characteristics have been studied on Daejappong which is autotetraploid brooded for mulberry fruit and Garmbacppong selected from the wild mulberry of Turkey. The number of chromosomes of Daejappong was 2n=56 recornized tetraploid, and that of Gambacppong was 2n=42 as a triploid. The percentage of seed formation, sank seed and germination of Daejappong were 48.2%,32.7%,90.0% respectively, on the other hand these of Carmbacppong were very low level as 41.0%, 1.9%,7.0% respectively. On the single fruit (sorosis) weight, Daejappong was large fruit as 4.39 g with light purple color, and Garmbacppong was medium fruit as 3.26 g with milk white color. Sugar contents (Brix %) and total acidities of Daejappong and Garmbacppong were 14.2%,0.45%, and 19.3%,0.15% respectively. Maturities of 2 cultivars were late and mulberry fruit yield of Daejappong was high and that of Garmbacppong was medium yielding.
Chracteristics of Mulberry Fruits on Daeseongppong, Daebungppong, Daeokppong and Shingwangppong(Morus Spp.)
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 99~103
Daeseongppong, Daebungppong, Daeokppong were bred, and Shingwangppong was selected among the recommended mulberry varieties of the grown silkworm, in order to meet the increasing the interest about the mulberry fruit, The major characteristics of the mulberry fruits were also studies. The chromosome number of Daeseongppong, Daebungppong and Daeokppong are 2n=56 recognized autotetraploid, and that of Shingwangppong is 2n=42 as a triploid. Seed forming rate of Daeseongppong, Daebungppong, and Daeokppong are more than 58%, but that of the Shingwangppong is very low with 6.7%. Concerning the single fruit weight, Daeseongppong is 4.05g, Daeokppong 3.38g, Daebungppong 2.99g which belonged to big fruit group. However Shingwangppong is medium as 2.28g. The sugar contents of Shingwangppong, Daeokppong, Daebungppong, Daeseongppong are 14.8%,13.7%,13.1% and 12.7% respectively. The sugar/acidity rate of them are above 21. The fruit maturity of Shingwangppong is early, those of Daebungppong, Daeokppong are medium and that of Daeseongppong is late. Yielding Potentiality of Daebungppong is high, those of Daeseongppong, Shingwangppong are comparatively high, and that of Daeokppong is moderate. In the case of cold hardiness, those of Shingwangppong, Daeokppong, Daebungppong are comparatively good, that of Daeseongppong is weak slightly.
Effect of Methanol Extract from Mulberry Fruit on the Lipid Metabolism and Liver Function in Cholesterol-Induced Hyperlipidemia Rats
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 104~108
We conducted this study to investigate the effect of methanol extract from Mulberry Fruit on the lipid metabolism and liver function in cholesterol-induced hyperlipidemia rats. We used 5 Mulberry Fruit varieties, including Daeryukppong, Kuksang 20, Subongppong, Wild variety and Cataneo. The yield of methanol extract from freezing dried Mulberry Fruits was 43.5-64.6%. Animal treated with Wild variety from Mulberry Fruit showed the decrease in body weight, food efficiency ratios, serum triglyceride (85%), and GOT value (86%). Our results suggest the hypolipidemic action of Wild variety from Mulberry Fruit.
Bioactive and Skin-compatible Properties of Silk Sericin
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 109~115
Silk sericin protein was extracted by treatment with enzyme or NaOH solution from raw silk and culled-cocoon shell. The extracted sericin was characterized and examined the functional properties as well as subjective properties for its use as a soap and a body cleaner. The optimum extraction conditions on specimen are NaOH (0.02 wt.%) or enzyme, Flavourzyme 3% under N2 gas. Molecular weights of sericin was decreased by treatment with enzyme, Actinase, from 10,000-30,000 to 2,700-4,200. Sericin showed important bioactive properties, for instance, lowering effect on blood glucose and alcohol. Subjective test of sericin soap and body cleaner showed superior in washability, foamability, and skin hydration, etc., to commercial soap and body cleaner. Therefore, it is thought that silk sericin can be expected as the source of bioactive and skin-compatible materials.
Phosphorylation of silk fibroin and its properties
Journal of Sericultural and Entomological Science, volume 43, issue 2, 2001, Pages 116~124
To improve the functional properties as a food, silk fibroin was phosphorylated with STMP In the phosphorylation reaction of silk fibroin, the degree of phosphorylation was increased with high alkali index and treatment temperature. Depending on treatment time and concentration of STMP it was rapidly increased up to 1hr. and 50%, but slowly above that time and 100%. It was indicated in the results of FT-IR analysis and
31/p NMR spectroscopy of phosphorylated fibroin that it had a close ∝-helix and poly-phosphate structure. The more phosphorylation of fibroin made more turbidity, foam expansion and foam stability, but less solubility. Emulsifying activity was increased up to P100, but slightly decreased above Pl00 and emulsifying stability was constantly increased on the progressing of phosphorylation.