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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 44, Issue 2 - Dec 2002
Volume 44, Issue 1 - Mar 2002
Volume 4, Issue 2 - 00 2002
Selecting the target year
Breeding of Cold Resistant Mulberry Cultivar “Hansungppong” (Morus alba L.)
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 45~50
New mulberry cultivar, Hansungppong, was bred among selected lines from F1 seedlings obtained by crossing the female, Yongcheonppong, and the male, Kugsang 21. Local adaptability test has been carried out at two places (Suwon and Chunchun) for four years since 1995. Leaf quality was tested by silkworm rearing for two silkworm rearing seasons (spring and autumn) at Suwon in 1998. Hansungppong was recognized to be superior in productivity and cold resistance to control cultivar, Yongcheonppong. The new cultivar, Hansungppong, has following agronomic characteristics as compared with Yongcheonppong, which was strongest leading cultivar against cold injury in Korea. It is belonging to Morus alba L. with the ovate leaf shape, sprouting in spring nearly the same to the Yongcheonppong (Morus alba L.), high leaf yielding of 10% compared to Yongcheonppong (Morus alba L.) and the high degree of cold resistance. It is adaptable to everywhere in Korea. Hansungppong is recommended to rear silkworms not only in spring but also in autumn besides for young silkworms.
Natural Distribution of Morus tiliaefolia Makino and Morus mongolica C.K.Schneider Growing Wild in the Korean Peninsula
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 51~55
The natural distribution of Morus tiliaefolia Makino and Morus mongolica C.K. Schneider growing wild in the Korean peninsula are as follows. The Morus tiliaefolia Makino is distributed in mountain areas in land and islands with along seaside northernmost at 30°8'North latitude, Hwacheon, Kangwon-do and it also unusually exists at the mountain Chilbo in Myeongcheon-gun, Hamgyeongbuk-do. On the other hand, the Morus mongolica C.K. Schneider is distributed westside the great mountainrange of Baeckdoo further-north to Jangsoo-mieon, Jaeryeong-gun, Hwanghae-do (38°15'N.), but it also unusually survives at the area of Samcheok, Kangwon-do (37°24')
Breeding of an Autotetraploid, Sawonppong 28, Derived from a Thick Leaf Mulberry Variety, Huyeoprok (Morus sup.)
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 56~58
An autotetraploid, Sawonppong 28 was derived from a thick-leaf and highly productive mulberry variety, Huyeoprok, by colchicine solution treatment on growing point and its characteristics were as follows. The number of somatic chromosomes of the derived variety was 2n=56 of an autotetraploid and green tip sprouting stage was medium budding variety with triangular oval shape and thick leaves contained rich chlorophyll. The length of branches was comparatively short and the number of branches was also small. However the branches were thick and non-budding length percentage at basal part of branch was high and cold hardiness was weak.
Phylogenetic Relationships of Morus Species on the Basis of RAPD
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 59~63
Phylogenetic relationships among mulberry varieties (Morus species) were analyzed on the basis of RAPD in order to identify the possibility of classification for the species. Polymorphisms under RAPD method were compared among 41 mulberry varieties. From the results of RAPD for 41 mulberry varieties by use of 30 different primers, 151 polymorphic bands were formed out of 201 ones. Under the dendrogram based on cluster analysis with the polymorphic bands, the varieties were classified into 7 groups including two large and five small ones on 0.747 value of genetic similarity coefficient. In the large groups 19 and 16 varieties were belong to group I and III, respectively. On the other hand, relatively high genetic similarity was shown among the varieties belonging to the group I, II and III. While, relatively low similarity were done between them in the group IV-Ⅵ and the other groups, and Mohusang in the group Ⅶ showed the lowest phylogenetic relationship with the other varieties.
Characterization and Expression of Antibacterial Protein Gene, Nuecin
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 64~68
The antibacterial protein gene, nuecin was expressed in Sf9 cells using baculovirus expression vector system (BEVS). The antibactetial activity of mature nuecin against Pectobacterium carotovorum subsp. carotovorum, Ralstonia solanacearum and Pseudomonas tolaasii was significantly high, demonstrating that nuecin had a wider antibacterial spectrum on gram negative and positive bacteria. The result appears to be superior to other antibacterial peptide, attacin. The nuecin was purified by SP-sepharose and Mono Q HR ion-exchange chromatography, and then by Superdex 200 HR 10/30 column. The purified nuecin is quite stable at 80
for several hours of incubation and in a wide pH range (pH 2-12).
Expression of Antibacterial Protein, Nuecin, Using Baculorivus Expression Vector System in Bm5 Insect Cell and Bombyx mori
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 69~73
For the practical use of nuecin protein, we tried to overexpress nuecin using Bm5 insect cell and Bombyx mori. We inserted nuecin cDNA into pBm10po1-Xa vector derived from B. mori nuclear polyhedrosis virus (BmNPV), and expressed in Bm5 cells and B. mori respectively. SDS-PAGE and Northern blot analysis showed an expressed of the protein when baculovirus expression vector system (BEVS) was used. The amount of intracellular protein is abundant, but the amount of extracellular protein is poor. The results suggest that the biologically active nuecin protein produced by using BEVS is poor because incresed level of misfolded nuecin by the strong promoter, polyhedrin and p 10 of BEVS, decrease the level of free chaperons and foldases by binding with them.
Artificial cultivation of Medicinal Mushroom, Phellinus linteus using Mulberry logs
Hong, In-Pyo ; Sung, Gyoo-Byung ; Chung, In-Mo ; Lee, Won-Chu ;
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 74~81
The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30
and pH 6.0, respectively. The mycelial growth of P. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose, mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of P. linteus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich. The initial mycelial growth and the full mycelial colonization of P. linteus were the best on 20 cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35
and over 96% of relative humidity.
Characteristics of Silk Sericin Powder Prepared by Mechanical Treatment
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 82~86
Silk sericin powder was prepared by mechanical treatment from culled-cocoon extract of Bombyx mori silkworm. The physical and structural characteristics of sericin powder were investigated by instrumental analysis. The solubility of sericin powder was decreased nearly half of sericin lyophilized. Amino acid analysis showed that arginine content was decreased from 3.72% to 0.03∼0.07%. XRD showed that molecules of sericin were somewhat regularly arranged by mechanical treatment. On the other hand, FT-IR showed the molecular vibration of silk sericin was not induced by mechanical treatment. Thermal properties also did not changed.
Dissolution and Characteristics of Antheraea pernyi Silk Fibroin Regenerated from Zinc Chloride Solution
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 87~92
Dissolution of Antheraea pernyi silk fiber was carried out in a zinc chloride solution with various dissolving conditions. The solubility was significantly dependent on the concentration of zinc chloride, dissolving temperature and time. The proper conditions of dissolution were found as 8 M zinc chloride, 70
temperature and 30 min dissolving time. Regenerated A. pernyi silk fibroin powder was obtained through dialysis. FTIR and XRD showed that regenerated A. pernyi silk powder was composed of a
-sheet as well as an
Effect of Reducing Agent, Sodium Hydrosulfite on the Natural Indigo Dyeing of Silk Fabric
Journal of Sericultural and Entomological Science, volume 44, issue 2, 2002, Pages 93~98
Effect of reducing agent sodium hydrosulfite on the natural indigo on the silk fabrics were examined to improve traditional dyeing method. K/S values of dyed fabrics was monitored with various dyeing temperature time, amount of dye and reducing agent. The dyeability of silk fabrics was improved by using reducing agent, sodium hydrosulfite; K/S value (7.20) was higher then that (1.09) of traditional method at the frist dyeing. Natural indigo dye extracted from dyed silk fabrics are composed of isomer, indigo (67.3%) and indirubin (32.5%). However, silk fabrics showed excellent anti-microbial activity regardless of the dyeing methods.