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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Sericultural and Entomological Science
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 45, Issue 2 - Dec 2003
Volume 45, Issue 1 - Jun 2003
Selecting the target year
Quantification of Cyanidin-3-glucoside(C3G) in Mulberry Fruits and Grapes
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 1~5
This study was carried out to investigate the anthocyanin pigments in the fruits of mulberry and grape. The anthocyanin pigments in the fruits of mulberry and grape were extracted with l% methanolic hydrochloric acid. The cyanidin-3-glu-coside (C3G) was separated and quantified by HPLC system using a Nova-Pack C
18/ column. The cyanidin-3-glucoside (C3G) contents of mulberry fruits were higher than that of grapes. Especially, anthocyanin pigments of mulberry fruits showed only C3G peak, but anthocyanin pigments of grapes showed some species peaks.
The Developmental Characterization of the Sericin jam(Bombyx mori), a Mutant in the Synthesis and Secretion of Fibroin
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 6~9
This experiment was done to know developmental characteristics of Sericin jam. Incubation periods were 10 day 2 hr, and 10 day 1 hr. 11 day 1 hr for Nd-s jam, N
jam, and Baegok jam, respectively. Hatching rates were 83.9, 83.3 and 96.0% for Nd-s jam, N
jam, and Baegok jam. Larval periods were, 20 days 1 hr for Nd-s jam, 20 days 5 hrs for N
jam, and 22 days 12 hrs for Baegok jam. Death rate of larvae was highest in N
iam, followed by Nd-s jam and Baegok jam. Pupation rate was highest in Baegok Jam followed by Nd-s jam and that of N
jam was the lowest among the three. Cocoon weight was 1.39, 1.08, and 2.01 g for Nd-s jam, N
jam, and Baegok jam, respectively. Shell weight were 13, 3, and 48 cg for Nd-s jam, N
jam, and Baegok jam. Cocoon shell ratios were 9.0% for Nd-s jam, 2.8% for N
jam and 23.9% for Baegok jam. Cocoon sizes were 30.6
15.8 mm for Nd-s jam, 24.7
14.9 mm for N
jam and 35.8
w) for Baegok jam.w) for Baegok jam.
Physicochemical Characteristic of the Silkworm Sericin Cocoon
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 10~17
This study was carried out to analyze physicochemical characteristics of sericin cocoon from silkworm, Bombyx mori. The degumming loss increased with increasing treatment time up to 2 hr, and temperature up to 130
. At 130
, degumming loss of Nd-s jam and Nd
H/ jam were 100% while that of Baegok jam was 24%. Nd-s jam and Baegok jam ha high glycine content of 29.1∼46.3 mol% where as Nd
H/ jam had high serine content of 32.6 mol%. Thermal denaturation temperatures were found at 218
for Nd-s jam, 216
H/ jam, and 218
for Baegok jam. Before degumming, crystallinities obtained by FT-IR analysis were 44.3, 43.7, and 59.9% for Nd-s jam, Nd
H/ jam, and Baegok jam respectively. After degumming, crystallinity increased to 61.8% for Baegok jam. Before degumming, crystallinitics obtained from XRD were 35.9, 33.5, and 47.2%, for Nd-s jam, Nd
H/ jam, and Baegok jam. After degumming, crystallinity increased to 49.8% for Baegok jam. The molecular weight of Nd
H/ jam were 9,417 in 1 hr, 3,744 in 2 hr, 4,944 in hr, and 3,910 in 6 hr.
Phylogeny of Bombyx mandarina inhabiting Korea analysing the isozyme and hemolymph protein polymorphism
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 18~24
B. mandarina of Korean population apparently differs B. mori in isozyme analysis. Fourteen polymorphism occurred B. mandarina not in B. mori at 6 isozymes, Bph, Bes, Amy-hc, Ies, Ict-D, Ict-E. Korean population has shared with the Korean native strain of B. mori in B genotype of Bes, F of Amy-hc, n of Ict-E, M and S of Ict-H. These 5 genotype were known that detection only Korean native strains of B. mori. Nei's genetic distance based on the genotype of isozyme and hemolymph protein using 4 populations of B. mandarina varied from 0.0350 to 0.0624. The distances of 0.0350 is between Jinju and Chilgok population and between Jinju and Kosung population has the largest distances, 0.0624. In genus of Bombyx, B. mandarina and B. mori, genetic distance varied from 0.3822 to 0.5074. Phylogenetic tree obtained using the subprogram UPGMA of NTSYS represented that Bombyx devided two group, B. mandarina and B. mori. B. mandarina has genetic differences according to the population within the Korean peninsula, but that was not recognized genetic variation or divergence considering low values of genetic distance.
A useful material isolation from the Bombycis corpus (Beauveria bassiana) growing of the silkworm, Bombyx mori L.
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 25~30
This study was carried out to investigate active constituents of Bombysis corpus on the neurite outgrowth from PC 12 cells led to isolate three new and a known sphingolipids from the n-hexane soluble portion and five amines from the butanol soluble portion of its methanol extract. On the basis of spectroscopic data, their structures have been elucidated as (4E,6E,2S,3R)-2-N-eicosanoyl-4,6-tetradecasphingadienine, (4E,2S,3R)-2-N-eicosanoyl-4-tetradecasphingenine,(4E,6E,2S,3R)-2-N-docosanoyl-4,6-tetradecasphingadienine,(4E,6E,2S,3R)-2-N-docosanoyl-4,6-tetradecasphingadienine,(4E,2S,3R)-2-N-octadecanoyl-4-tetradecasphingenine, 1,7-dimethyl-xanthine, uracil, urea, betaine and tyrosine, respectively. The neurite outgrowth activities of these compounds were examined in PC12 cells by measuring the length of neurites. These compounds promoted neurite outgrowth in PC12 cells significantly.
Study of optimal pathogenicity condition on cordyceps
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 31~33
This study was carried out to investigate pathogenicity of liquid spawns on dongchunghacho growing using silkworm, optimum infective condition for mass production and infection rate on various conservation periods and temperatures. We compared with the infection rates after conservation the liquid spawns (Paecilomyces tenuipes) at 4
for 10 days and 20 days. In these results, infection rate of conservated liquid spawns at 4
was 88.0 percent in the tenth day, and then again it was 5.3 percent at 25
. Infection rates at 4
were on the whole excellent out of long-term preservation methods.
Degumming of Silk by Calcium hydroxide
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 34~45
Silk was treated with calcium hydroxide for degumming at different treatment times, temperatures and Ca(OH)
concentration to optimize degumming conditions in this thesis. After degumming, soluble and insoluble sericin were seperated and then the soluble sericin was characterized by measuring the average degree of polymerization (D.P.), lysinoalanine (LAL) content, DSC, and by amino acid analysis. And degummed silk fibroin was characterized by measuring tenacity and SEM. Degumming loss was increased by increasing the treatment time and temperature until about 30 minutes. After then, a slight difference was found along with treatment times at the Ca(OH)
concentrations of 0.07% and 0.1% solutions. After degumming, insoluble sericin ratio on degumming solution was increased by increasing treatment temperature at Ca(OH)
0.04% solution. At the concentration Ca(OH)
of 0.07%, a soluble ratio was almost 100% regardless of treatment time and temperature. At the beginning of treatment, insoluble ratio was high at Ca(OH)
0.1% solution but it was decreased by increasing treatment time. At the Ca(OH)
concentration of 0.04%, D.P. of soluble sericin was maintained as a constant value of 10 at 100
although treatment time was increased. However, at 80
, it was hard to prepare a soluble sericin having a constant D.P. by increasing treatment time. At the Ca(OH)
concentration of 0.07%, D.P. was almost 10 irrespective of treatment temperature and time. Soluble sericins with high D.P. of 20∼30 were obtained at 0.1% and 100
. LAL was not detected in soluble sericin. As the results of amino acid analysis, it showed that Ca(OH)
degumming reduced the contents of hydroxy amino acids like Ser., Thr. and Tyr. In DSC analysis of soluble sericin, endothermic peak by thermal deformation and pyrolysis showed at 189
and at 299
, respectively. The tenacities of degummed silk were 15∼30% lower than that of raw silk. And it was decreased with increasing treatment time. From the morphological study, the thickness of degummed silk fibroin became thinner by increasing degumming loss. The roughness of a silk fibroin surface was appeared as treatment concentration was increased.
Hydrolysis characteristics and applications of silk sericin I. Control of molecular weight of sericin
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 46~57
This study was carried out to investigate the characteristics of the soluble sericins after degumming and after hydrolysis of insoluble sericin with various enzymes. Especially, the hydrolysis characteristics were examined in terms of molecular weight of the soluble sericin. Amino acid composition and molecular weight characteristics of the soluble sericins were also studied. When the insoluble sericin was hydrolyzed with kojizyme and flavourzyme, the solubility was highest at pH 7 and 50
. On the other hand, in the cases of protamex and alcalase, the highest solubility was obtained at 60
. In these cases, solubility increased with pH. In enzymatic hydrolysis, the solubility was increased with concentration of enzymes until 4 hours. After then, a slight difference was found along with treatment times. In enzymatic hydrolysis, the absorbance of the soluble sericin was increased with concentration of enzymes and treatment times. Average degree of polymerization was decreased with treatment time and concentration. The amino acid compositions were similar in low(low molecular weight by degumming) and high (high molecular weight by degumming). Those of PK (soluble sericin hydrolyzed with kojizyme), PP (soluble sericin hydrolyzed with protamex), and PA(soluble sericin hydrolyzed with alcalase) were similar to each other. Serine and tyrosine compositions were higher in low and high than those of PK, PP, and PA. However proline was absent in low and high. Molecular weights of the various sericins became higher as KP>high>PP>low>PA and those of KP and PA were 9,800 and 905 respectively.
Hydrolysis characteristics and applications of silk sericin II. Characteristics of soluble sericin and its application on human skin
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 58~65
This study was carried out to investigate the characteristics of the soluble sericins after degumming and after hydrolysis of insoluble sericin with various enzymes. Physical and chemical characteristics of the soluble sericins were also studied and pack test was conducted. Electro donating ability of the various sericins became higher as high＞low＞KP＞PP＞PA. In FT-IR analysis, the band of insoluble sericin at 3,285cm
-1/ was shifted to the lower wavelengths in soluble sericins. The intensity of the amide II band was reduced and shifted to lower wavelengths as a result of hydrolysis. In DSC analysis, all sericins showed endothermic peaks around 200
and insoluble sericin showed another peak at 250
. The endothermic peak of the insoluble sericin was found at higher temperature compared with those of the soluble sericins which showed at 300
. Whiteness of the various sericins became higher as low＞high＞PP＞PA＞PK＞IN and yellowness were PK＞PA＞PP＞low＞high. In pack test, the skin packed with the non-woven fabric treated with the various sericins was more smooth and less oily.
Dyeing of Silk Fabric with Persimmon Extract
Journal of Sericultural and Entomological Science, volume 45, issue 1, 2003, Pages 66~70
Silk fabrics were dyed with persimmon dyeing solution. The persimmon extracts were obtained with boiling water from persimmon fruits and leaves. The changes of K/S values were investigated according to the concentration of solution, the ripeness of persimmons and the method of mordanting. The colours of the fabrics differed according to the kinds of mordants. The mordant, FeSO
, was more effective than other mordants. The physical properties were not changed hardly after the natural dyeing with persimmon extracts.