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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 49, Issue 2 - Dec 2007
Volume 49, Issue 1 - Jun 2007
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Germ Line Transformation of the Silkworm, Bombyx mori L. with a piggyBac Vector Harboring the Human Lactoferrin Gene
Kim, Yong-Soon ; Sohn, Bong-Hee ; Kim, Kee-Young ; Jung, I-Yeon ; Kim, Mi-Ja ; Kang, Pil-Don ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 37~42
Lactoferrin, an ion-binding 80-kDa glycoprotein, has been suggested to have many biologic activities, such as facilitating ion absorption and having antimicrobial and anti-inflammatory effects. Several of these activities are likely to only be facilitated by human lactoferrin because they depend on the binding of human lactoferrin to specific receptor. To produce recombinant human lactoferrin to animal foods using transgenic silkworm, Bombyx mori L, we have cloned and sequenced the cDNA encoding for a human lactoferrin (HLf) from the mRNA in mammary tumor line (GI-101). As a result, the 2.5-kb fragment of HLf gene was cloned with pGEM-T vector and then this fragment was sequenced. In the nucleotide sequence analysis, single open reading frame of the 2,136-bp encoding for a polypeptide of 712 amino acid residues was detected. On the other hand, we constructed a recombinant plasmid(pPT-HLf), containing human lactoferrin gene for germ line transformation of the silkworm using a piggyBac transposon-derived vector. A nonautonomous helper plasmid encodes the piggyBac transposase. Approximately 6.7% of individuals in the G0 silkworms expressed green fluorescent protein (GFP). PCR analyses of GFP-positive silkworms (G0 and G1) revealed that independent insertions occurred frequently. Furthermore, Western blot analysis showed that the recombinant HLf expressed in hemolymph has the same molecular weight (80 kDa) as a native protein. On the basis of these experiments, expression of HLf in next generation of transgenic silkworm is now in process.
Analysis of General Ingredient and Amino Acid Contents in Silkworm Feces with Different Extracting Methods and Collecting Time
Sohn, Bong-Hee ; Chung, Wan-Tae ; Kang, Pil-Don ; Ryu, Kang-Sun ; Jung, I-Yeon ; Kim, Yong-Soon ; Kim, Kee-Young ; Kim, Mi-Ja ; Chung, In-Sick ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 43~46
Analysis of general ingredients and amino acid content in dried silkworm feces which were extracted with hot water, EtOH, MeOH for developing new income source of rearing farm, seeking high additive value material and industrialization of silkworm feces. In the analysis of general ingredient content by extracting solution, raw protein and ash contents were increased with MeOH, EtOH, hot water. And raw fat showed higher contents by the order of EtOH > MeOH > hot water. The inorganic matter content showed a little difference among collection times of silkworm feces. Hot water was most effective extracting solution for total amino acid, and no significant difference between EtOH and MeOH. According to the time, there were no significant difference in amino acid contents. When extracted with hot water, Glu content was highest content and order of others is Asp > Gly > Ala > Leu > Thr > Ser among 18 amino acids.
Selection of Mutant Silkworm with Oxidation-deficient Haemolymph for Insect Cell Culture
Choi, Ji-Young ; Kim, Jong-Gill ; Choi, Young-Cheol ; Yoon, Hyung-Joo ; Ahn, Mi-Young ; Kim, Sam-Eun ; Hwang, Seok-Jo ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 47~50
Insect cell culture system has been demonstrated the effective means of producing medical and agricultural products. Furthermore, Fetal bovine serum (FBS) is in wide use in insect cell culture. Silkworm hemolymph was tested to develop as a substitute for FBS and was effective in insect cell growth. Hemolymph is oxidized and darkens visibly during the collection from silkworms due to the activity of tyrosinase in it. Toxic quinones are produced by the oxidation and consequently inhibit the cell growth. Heat treatment can be used to prevent the oxidation; however, the oxidation may occur during the collection of hemolymph before it is heat-treated. Hemolymphs collected from 257 different strains of silkworms were examined to select the slowly oxidized hemolymphs. Hemolymphs collected from mutant strains such as
, TBO and
showed relatively slow color changes. Oxidation rates of the hemolymphs were measured by the absorbance change using a spectrophotometer. The absorbance of mutant hemolymph reached the saturation value at
in each 330 min (
), 360 min (TBO) and 450 min (
) min, whereas the total oxidation time of the wild-type (Baekokjam) hemolymph at the same temperature was 120 min. The cell growth in the medium supplemented with mutant species hemolmph was more effective that in the medium supplemented with Baekokjam species hemolymph.
Effects of Atmospheric factors on Local Adaption Rearing Test Results of Superior Breeding Combination of Silkworms
Sohn, Bong-Hee ; Kang, Pil-Don ; Ryu, Kang-Sun ; Jung, I-Yeon ; Kim, Yong-Soon ; Kim, Kee-Young ; Kim, Mi-Ja ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 51~55
Investigation of atmospheric data and rearing results was conducted to analyze the effects of atmospheric factors such as temperature and precipitation on silkworm in 8 rearing places in which local adaptation test was being conducted with different mulberry growth condition, soil and atmosphere during spring and autumn rearing season of 2006. The atmospheric characteristics of spring rearing time are as follows. The average temperatures of young silkworm, old silkworm, mounting were
higher than normal year. The precipitation of young silkworm, old silkworm, mounting were 15.1 mm, 6.9 mm, 7.0 mm, respectively, and 22.9 mm lower than normal year in old silkworm and mounting. The daylight hours in larval stage was 1.7 hour shorter than normal year, but no difference in mounting. Thus precipitation was lower and temperature was higher than normal year in 2006. The rearing results of 2006 were 1 kg lower than normal year in cocoon yields per 10,000 3 rd molted larvae, single cocoon weight and cocoon shell percentage were a little higher. The atmospheric characteristics of spring rearing season are as follows. The average temperatures of young silkworm, old silkworm, mounting were
respectively, temperature in young silkworm was
higher than normal year, and temperature in old silkworm was
lower than normal year. The precipitation of young silkworm, old silkworm, mounting were 110.2 mm, 4.6 mm, 3.7 mm, respectively and there were little differences compared to normal year. The atmospheric condition of 2006 which was similar to normal year did not affect the autumn rearing results of 2006. Namely, the single cocoon weight and cocoon shell weight were not different from normal year, and the same was cocoon shell percentage.
Characteristics of Newly Bred Mulberry Cultivar "Daesungppong" (Morus Lhou(Ser.) Koidz.) for Mulberry Fruit Production
Sung, Gyoo-Byung ; Kim, Hyun-Bok ; Hong, In-Pyo ; Nam, Sung-Hee ; Chung, In-Mo ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 56~59
We bred new mulberry cultivar through local adaptability test, which was registered as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Konju and Cheongju) for three years since 2002. This is tetraploidy variety belonging to (Morus Lhou(Ser.) Koidz.) made by colchicine treatment on growing point of winter buds. Daeseongppong was high yielding in productivity by 48% compared to control cultivar "Chungilppong" for two years. Although Daesungppong was a little lower in sugar content of mulberry fruits, it contains more bioactive materials like C3G, rutin and amino acids than Chungilppong. It is adaptable to every where except the places where cold damage and sclerotial disease happen frequently.
Agronomic Characteristics and Anti-oxidant Capacity of Mulberry Genetic Resources conserved by Jeollabuk-Do
Kim, Hyun-Bok ; Koh, Seong-Hyouk ; Oh, Nam-Ki ; Jeong, Jong-Seong ; Sung, Gyoo-Byung ; Hong, In-Pyo ; Chung, In-Mo ; Lee, Kwang-Gill ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 60~66
This study was carried out to develop the utilization of mulberry resources conserved by Jeollabuk-Do Agricultural Research & Extension Services. Mulberry accessions were tested for agronomic characteristics and antioxidant capacity according to varieties. From that result, three suitable varieties such as 'Sinilppong', 'Suwonsang 2' and 'Ilbongeum' were selected for the production of mulberry leaves. They have strong agronomic characteristics like size, yield and resistance against damages by blight and harmful insects. Whereas, 'Gumunyoung' showed the lowest freezing resistance. In the antioxidant capacity analysis of mulberry leaves, autumn's mulberry leaves showed higher antioxidant capacity (ascorbic acid equivalent) than that of spring's it. According to collection time, antioxidant capacity were 2,109.8 nmol (August), 2,617.8 nmol (September) and 3,311.5 nmol (October), respectively.
Dietary Effect of Silk Protein on Colon Cancer of Animal
Yeo, Joo-Hong ; Lee, Kwang-Gill ; Woo, Soon-Ok ; Kweon, Hae-Yong ; Han, Sang-Mi ; Park, Kyung-Ho ; Kim, Sung-Su ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 67~70
This study was done for the dietary effect of silk protein, molecular controlled silk sericin and silk fibroin, on colon cancer of animal. The ICR mice were given weekly injections of 1,2-dimethylhydrazine (DMZ) for the initial 10 mg/kg with four weeks, and fed a diets supplemented with 3% (v/v) molecular controlled sericin and fibroin for 6 weeks, respectively. Then, the body weight, efficiency of dietary, typical histologic appearance of animal colon tissue and COX-2 effect were studied, too. Molecular controlled sericin protein are shown more higher dietary effect on animal colon cancer than fibroin supplemental case. The results suggest a potential usefulness of sericin as a chemopreventive for colon carcinogenesis and the development functional food.
Production of Colored cocoons from Silkworm by Feeding Artificial Diet mixed Dye
Chung, In-Mo ; Sung, Gyou-Byung ; Kang, Pil-Don ; Kim, Mi-Ja ; Nam, Sung-Hee ; Hong, In-Pyo ; Shon, Bong-Hee ; Kim, Hyun-Bok ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 71~73
In order to produce color cocoon, 3 rd day 5 th instar silkworm was cultivated with dye mixed artificial diet. Silkworm fed by artificial diet mixed with gardenia pigment spun blue colored cocoon, by Orange II orange cocoon, by methyl orange yellowish cocoon, and by Acid blue R bluish cocoon. Cocoon weight and cocoon shell weight ratio of silkworm fed by colored artificial diet were lower than those of normal silkworm.
Biological Activities of Antheraea yamamai Silk Fibroin Hydrolysates
Kim, Jong-Ho ; Shin, Bong-Seob ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 74~76
Antheraea yamamai silk fibroin powder was prepared by treatment with HCl. Amino acid contents of the prepared Antheraea yamamai fibroin hydrolysates were composed of Gly, Ala, Glu, and so on. Biological activities of Antheraea yamamai silk fiborin powder were examined. It is showed about 75% of antioxidation activity compared to the reference ascorbic acid. And it lowered blood glucose level down from 550 mg/dl to 300 mg/dl by serving with wild silk protein.
Differential Scanning Calorimetric and Thermogravimetric Analysis of Silk Fibroin / poly (Vinyl pyrrolidone)
Kweon, Hae-Yong ; Lee, Kwang-Gill ; Yeo, Joo-Hong ; Woo, Soon-Ok ; Han, Sang-Mi ;
Journal of Sericultural and Entomological Science, volume 49, issue 2, 2007, Pages 77~80
Silk fibroin/poly (vinyl pyrrolidone) conjugates were prepared and characterized through differential thermal calorimeter and thermogravimetry. The glass transition temperature (Tg) of poly (vinyl pyrrolidone) was not changed by reaction with silk fibroin. However, abnormal exothermic peak was observed at the silk fibroin/poly (vinyl pyrrolidone) conjugates. Thermogravimetric analysis showed that thermal stability of silk fibroin was relatively increased by reaction with PVP.