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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 51, Issue 2 - Nov 2013
Volume 51, Issue 1 - Apr 2013
Selecting the target year
Effects of rearing density on food consumption, adult mortality and mean number of hatchlings of Gryllus bimaculatus (Orthoptera: Gryllidae)
Park, Young-Kyu ; Lee, Hyung-Gun ; Choi, Young-Cheol ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 89~94
DOI : 10.7852/jses.2013.51.2.89
The aim of the present study is to investigate food consumption, hatching periods, adult mortality, the mean number of hatchlings and ovipositional trend of Gryllus bimaculatus De Geer. The rearing and experiments were conducted in the rearing room (
RH). As the adult rearing density increased from 50 to 400, it did not affect the average daily food consumption of adult crickets which was
g a day. However, the daily radish consumption decreased from 0.128 to 0.068 g per day. 95.8% of eggs hatched by the sixth day after the beginning of hatching. The average mortality of the adults gradually increased, and reached 93.9% by the 8th week. Total number of hatchlings per female was
) in 200 females rearing cages, which was noticeably less than
) in 100 females rearing cages. Sex ratio of the crickets given in the rearing cages did not affect both the number of hatchlings and ovipositon rate. Oviposition rate periodically fluctuated as progressed, and it reached 81.5% of the total oviposition by the
week since the beginning of ovipoisiton.
Nutritional value of black soldier fly, Hermetia illucens (Diptera: Stratiomyidae) as a feed supplement for fish
Park, Kwanho ; Choi, Youngcheol ; Nam, Sunghee ; Kim, Sunghyun ; Kim, Sinyoung ; Ma, Youngjoo ; No, Sikab ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 95~98
DOI : 10.7852/jses.2013.51.2.95
The experiment was conducted to determine the value of black soldier fly (Hermetia illucens) larvae and pupae as feed supplements for fresh-water fish (Carassius auratus). Black soldier fly larvae grown on food waste were fed to fresh-water fish, live larvae alone, dried larvae, dried pupae and combination with commercial fish diet. A 16-week feed trial was conducted to determine whether black soldier fly larvae could be used as a feed supplement in fresh-water fish. The growth performance indicated that fish fed the live larvae ranked consistently higher, and in combination with commercial fish diet and dried larvae and pupae were next higher. The finding of this study suggest that black soldier fly larvae and pupae may be valuable feedstuff in commercial fish production.
The effects of the mulberry and silkworm intake on androgen receptor mRNA and myogenic regulatory factors expression of rats muscle for resistance exercise
Yang, Sung Jun ; Kim, Chang Yong ; Lee, Jo Byoung ; Kang, Sung Sun ; Lee, Jong Jin ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 99~106
DOI : 10.7852/jses.2013.51.2.99
The purpose of this study is to investigate the effects of supplementation of mulberry powder, mulberry extract and silkworm powder during the 8 weeks of resistance exercise on Androgen receptor(AR) mRNA and Myogenic regulatory factors(MRFs) expression of rats muscle. Fifty males, Sprague-Dawley rat, were randomly divided into 5 groups: CON(control group, n = 10), REG(resistance exercise group, n = 10), MP REG(mulberry powder intake and resistance exercise group, n = 10), ME REG(mulberry extract intake and resistance exercise group, n = 10) and SP REG(silkworm powder intake and resistance exercise group, n = 10). After climbing the ladder without weights during the 1 week of adaptation period, the rats in the resistance exercise group were trained to climb a 0.98-m vertical(80 degree incline) ladder with weights in their tail during 7 weeks(10 times each day, 2 days per week). After exercise, the skeletal muscle was extracted from the flexor hallucis longus. After separating the total ribonucleic acid (RNA) of each group, quantitative polymerase chain reaction was used to analyze RNA quantitatively. AR mRNA and MRFs expression revealed that all of the treated groups had significantly difference. AR mRNA expression increased in ME REG
and SP REG
fold compared to CON. Myod mRNA expression increased in MP REG
, ME REG
and SP REG
fold compared to CON. And myogenin mRNA expression increased in MP REG
, ME REG
and SP REG
fold compared to CON. In conclusion, during the resistance exercise, providing mulberry and silkworm gives positive effect on AR mRNA and MRFs expression increase.
Effect Analysis of Compost Derived by Black Soldier Fly(Hermetia illucens L.) Using Plant Growth Analysis Method
Choi, Young-Cheol ; Park, Kwan-Ho ; Lee, Yonggu ; Moon, Sung-Kyoung ; Choi, Hansu ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 107~113
DOI : 10.7852/jses.2013.51.2.107
The black soldier fly larvae are able to decompose various organic wastes such as livestock manures and food wastes. We tested whether the quality of the insect derived compost, i.e. larval feces, was comparable to that of a commercial fertilizer. Chemical analysis of Hermetia. illucens compost was suitable as a fertilizer. When the kidney bean (Phaseolus vulgaris L.) was raised on the culture soil treated with the H. illucens compost, the growth of leaf area, total dry weight and leaf dry weight increased significantly. The H. illucens compost is thought to act as an increasing factor of RGR (relative growth rate) from the beginning of growth and also had a great effect on the relative growth rate throughout the late stage of growth. There is high statistical significance between NAR (net assimilation rate) and RGR (relative growth rate), but no significance between RGR and LAR (leaf assimilation rate) of the treatments. In addition, the treatment of H. illucens compost promoted the thickness of leaves from the beginning of growth and the tendency of thickening leaves from the beginning of growth and the effect continued throughout the late period of growth. When mixed with 50% of H. illucens compost rather than 25%, it showed the greater effect on the plant growth.
Effects of experience-based learning program using singing insects
Kim, So Yun ; Kim, Seong Hyun ; Jung, Jong Cheol ; Lee, Kwang Pum ; Kim, Nam Jung ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 114~118
DOI : 10.7852/jses.2013.51.2.114
This study examines how an experimental-based learning program using singing insects improves the academic achievement of elementary school children. Study subjects were a total of 123 elementary students at their
grade who voluntarily participated in an experience-based learning program organized by Seodaemun Museum of Natural History, Seoul. Students were given the same survey questionnaires before and after taking the learning program, and this procedure was repeated in 7 independent replication trials. Result from a paired t-test indicated that the learning program had a positive effect on academic performance, with students gaining significantly higher mean scores in the survey test after taking the program than before. This result suggests that the experience-based learning program using singing insects is effective at improving student's academic achievement. Our study provides a critical impetus for developing a variety of other experience-based learning programs using insects like ours, leading us to anticipate that these programs will be practiced more systematically and actively in the classroom in future.
The morphological characteristics and fatty acids composition of pollens in acorn and darae(Actinidia arguta)
Hong, In-Pyo ; Lee, Man-Young ; Woo, Soon-Ok ; Sim, Ha-Sik ; Choi, Yong-Soo ; Han, Sang-Mi ; Kim, Hye-Kyung ; Byeon, Kyu-Ho ; Lee, Myeong-Lyeol ; Kim, Jung-Bong ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 119~122
DOI : 10.7852/jses.2013.51.2.119
Pollens have been known to possess various biological properties. Therefore, pollens have been extensively used in functional food, folk medicine, and beverage industry to improve human health. This study was conducted to establish the optimized protocol for cytoplasm isolation of bee pollen. Data of biochemical parameters and fatty acid profiles were obtained from pollens of Acorn and Darae(Actinidia arguta). Contents of crude protein and crude fat were 24.1% and 11.8% in Acorn pollen, and those of Darae pollen showed 35.8% and 8.7% in crude protein and crude fat respectively. Also after lyophilizing of Acorn pollen, content of crude protein was increased to 26.5%. Main fatty acids were palmitic acid(C16 : 0), oleic acid(C18 : 1), linoleic acid(C18 : 2) and linolenic acid(C18 : 3) in bee pollen. Linoleic acid(37.3%) was dominant fatty acid in Acorn pollen that is one of essential fatty acids. Linolenic acid(48.3%) was dominant fatty acid in Darae pollen that is a polyunsaturated fatty acid. The proportion of unsaturated fatty acids to total acid content was 73.2% in Acorn and 63.2% in Darae pollen, and especially that of polyunsaturated fatty acids was higher than 55%.
The effects of the mulberry and silkworm intake on muscle increase of rats for resistance exercise
Yang, Sung Jun ; Kim, Chang Yong ; Lee, Jo Byoung ; Kang, Sung Sun ; Lee, Jong Jin ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 123~129
DOI : 10.7852/jses.2013.51.2.123
The purpose of this study is to investigate the effects of supplementation of mulberry powder, mulberry extract and silkworm powder during the 8 weeks of resistance exercise on muscle increase of rats. Fifty males, Sprague-Dawley rat, were randomly divided into 5 groups: CON(control group, n = 10), REG(resistance exercise group, n = 10), MP REG(mulberry powder intake and resistance exercise group, n = 10), ME REG(mulberry extract intake and resistance exercise group, n = 10) and SP REG(silkworm powder intake and resistance exercise group, n = 10). After climbing the ladder without weights during the 1 week of adaptation period, the rats in the resistance exercise group were trained to climb a 0.98-m vertical(80 degree incline) ladder with weights in their tail during 7 weeks(10 times each day, 2 days per week). After exercise, the skeletal muscle was extracted from the flexor hallucis longus. The absolute weight of skeletal muscle was measured by the electronic balance and the relative weight of skeletal muscle about the weight was calculated. The strength and density of legs muscle were analyzed by the computed tomography scan. After 8 weeks of resistance exercise, the absolute weight of skeletal muscle wasn't significant, but it increased in MP REG 8%, ME REG 8% and SP REG 10% compared to REG. The relative weight of skeletal muscle increased significantly in SP REG compared to REG (p < 0.05). Additionally, there was a significant increase of density and strength of the forelegs in SP REG compared to CON (p < 0.05). However, there was no significant increase of density and strength of the hind legs. In conclusion, regular resistance exercise made rats muscle increase and there was more muscle increase effected by providing silkworm.
Characteristics of mulberry cultivar Shimheung (Morus alba L.) for mulberry fruit production
Sung, Gyoo Byung ; Kim, Hyun-Bok ; Kang, Pil Don ; Kim, Kee Young ; Ji, Sang Duk ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 130~136
DOI : 10.7852/jses.2013.51.2.130
We bred a mulberry cultivar named Shimheungppong, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at three places(Suwon, Wanju, and Jinju) for six years from 2004. This is tetraploidy variety belonging to (Morus alba L.) selected among mulberry genetic resources preserved in Suwon. Shimheungppong was high yielding cultivar in fruit productivity by 5% compared to control cultivar 'Chungilppong(Morus alba L.)' for four years. Although Shimheungppong was a little lower in sugar content of mulberry fruits, it was bigger than that of Chungilppong. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.
Comparison of scanning electron microscopic structures and nucleotide sequences variation of ITS1, 5.8S ribosomal RNA gene and ITS2 region in three Peruvian entomopathogenic fungal isolates
Han, Sang-Hoon ; Nam, Sunghee ; Lee, Heui-Sam ; Yeo, Joo-Hong ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 137~141
DOI : 10.7852/jses.2013.51.2.137
In this study, nucleotide sequence structures of intergenic transcribed spacer (ITS) 1, complete 5.8S ribosomal RNA gene and ITS 2 region were analyzed to identify three Peruvian entomopathogenic fungal isolates. The isolates had highly conserved sequence region in 5.8S rRNA gene and unique sequences in ITS 1 and 2 region among them. 5.8S rRNA gene regions were highly conserved and showed high homoloies among tested isolates. In contrast, ITS region showed species-specific sequence region, resulting in inter-genus differencies. Scanning electron microscopic images of these isolates supported the result of ITS-based identification. From these result, Peruvian entomopathogenic fungal isolate J270, J278, were identified as Beauveria bassiana and J271 was identified as Lecanicillium attenuatum.
Reeling of recombinant flourescence cocoons through low temperature decompressed cooking
Park, Jong-Hwa ; Kim, Sung-Wan ; Jeong, Young-Hun ; Lee, Jong-Kil ; Go, Young-Mi ; Lee, Sang-Chan ; Choi, Kwang-Ho ; Kim, Seong-Ryul ; Goo, Tae-Won ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 142~146
DOI : 10.7852/jses.2013.51.2.142
The fluorescent proteins are generally denatured by heat treatment and thus lose their color. The normal reeling method includes processing by drying and cooking the cocoons near
before reeling. Therefore, the usual processing method cannot be used for making colored fluorescent silks. To develop a method that is applicable to producing transgenic silk without color loss, we develop reeling methods adequate for a recombinant fluorescence cocoons. It was found that the fluorescence cocoons keep their native color when dried at temperatures lower than
for 15 h. Also, a new cooking method to soften the fluorescent cocoons was developed: the cocoons were soaked in a solution of 0.2% sodium carbonate (
)/0.1% nonionic surfactant (Triton X100) at
and then placed under vacuum. The repeated vacuum treatments enabled complete penetration of the solution into the cocoons, and the cocoons were thus homogenously softened and ready for reeling. In this state, the cooked cocoons can be reeled by an automated reeling machine. Our results suggest that drying and cooking of the cocoons at low temperature enables the subsequent reeling of the colored fluorescent silks by an automatic reeling machine without color loss and can produce silks that can be used for making higher value-added silk materials.
Comparison of fibrinolytic activity from Korean indigenous insects
Kim, Hyunae ; Lee, Sang Han ; Choi, Youngcheol ; Park, Kwanho ; Hwang, Jaesam ; Kim, Namjung ; Nam, Sunghee ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 147~152
DOI : 10.7852/jses.2013.51.2.147
The fibrinolytic activity of aqueous extracts from Korean indigenous insects were studied. Fibrinolytic activity of aqueous extract from 5 insects (larva and adult parts of Meloimorpha japonica, Allomyrina dichotoma, Cetonia pilifera, Apis mellifera) showed 2.7-fold potent than that of plasmin used as a positive control. In addition, the fibrinolytic activity was observed through the degradation products by SDS-PAGE. The extracts efficiently hydrolyzed
chains of human fibrinogen. This study suggested that aqueous extracts from Korean indigenous insects have potential in developing a useful source of antithrombosis agent(s).
Production of fluorescent green silk using fibroin H-chain expression system
Kim, Seong Wan ; Yun, Eun Young ; Choi, Kwang-Ho ; Kim, Seong Ryul ; Park, Seung Won ; Kang, Seok Woo ; Goo, Tae Won ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 153~158
DOI : 10.7852/jses.2013.51.2.153
To express green fluorescent protein in the cocoon of silkworm, we constructed the fibroin H-chain expression system to produce enhanced green fluorescent protein (EGFP) in the cocoon of transgenic silkworms. The EGFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EGFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 1,200 eggs of bivoltin silkworms, Baegokjam. We obtained 8 broods. The cocoon displayed strong green fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and immunoblotting. Accordingly, we suggest that the EGFP fluorescence silk will enable the production of the novel biomaterial based on the transgenic silk.
Processing method of mulberry fruit juice improved C3G stability
Kim, Hyun-Bok ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 159~163
DOI : 10.7852/jses.2013.51.2.159
As mulberry fruit's functionalities are known to the media, the interest in promoting of consumption and processed products is increasing. But there is no C3G(Cyanidin-3-glucoside) data based on the stability of the pigment during processing. To solve this problems, and to expand the use of mulberry fruit, processing methods was developed for mulberry fruit juice improved cyanidin-3-glucoside(C3G) stability. The results obtained are summarized as follows. The food additive citric acid with 0.3 % improved C3G content and antioxidant ability in the treatment of mulberry fruit and sucrose the ratio of 50 % : 50 %(w/w). In the case of the addition of oligosaccharides, citric acid decreased antioxidant ability. Xylitol treatment showed up the lowest of C3 content, but by the addition of citric acid improved the pigment content and antioxidant activity of the mulberry fruit juice. Addition of citric acid was more affected C3G stability than containers (clear glass bottles, brown glass bottles, aluminum foil, green glass bottle, translucent glass bottles). In the processing of mulberry juice, 3 minutes blanching treatment using microwave dropped C3G content somewhat. Therefore, using this method is not recommended. However in the antioxidant ability, microwave blanching showed a stabilizing effect compared to the other treatments.
Functional quality characteristics of extracts by sugar-leaching and lactic acid fermentation of mulberry leaves (Morus alba L.)
Ryu, Il-Hwan ; Kwon, Tae-Oh ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 164~172
DOI : 10.7852/jses.2013.51.2.164
This study was carried out to investigate functional quality characteristics of extract obtained after sugar-leaching for 12 weeks (SLE) and extract obtained after lactic acid fermentation for 8 weeks (LFE) of mulberry leaves. The yield, sugar content, pH, and total acidity of SLE were 27%, 43
, 4.6, and 0.45%. The yield, sugar content, pH, and total acidity of LFE were 166%, 33
, 3.6, and 1.17% respectively. The lactic acid bacteria viable numbers (
CFU/ml) of LFE were more than those of SLE (
CFU/ml). The LFE expressed activities of hydrolytic enzymes (amylase, cellulase, pectinase, protease), but SLE did not express. The contents of acetic acid, citric acid, and malic acid of SLE were higher than those of LFE, but lactic acid content of LFE was higher than that of SLE. The main free sugars of SLE were glucose (200.93 mg/g), fructose (236.32 mg/g), and sucrose (18.41 mg/g), but LFE did not detect all free sugars. The contents of polyphenol, anthocyanin, and piperidine alkaloid of LFE were higher than those of SLE.
-Glycosidase activities were inhibited 3.4% and 16.2% by SLE and LFE. These results suggest that lactic acid fermentation extraction is an effective method to increase the yield and contents of functional quality of mulberry leaves extract.
Studies on bionomical characteristics of Papilio xuthus
Lee, Sang-Hyun ; Kim, Se-Gwon ; Kim, Nam-Ee ; Bae, Kyoung-Sin ; Choi, Young-Cheol ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 173~179
DOI : 10.7852/jses.2013.51.2.173
This study was investigation for primary bionomical characteristics, emergence rate of wintering type pupa and oviposition preference, of Papilio xuthus. The emergence rate of wintering type pupa stored at cold chamber(
) was 85%, after 10 months. The average emergence rate of wintering type pupa during storage was 89.6%, and the emergence periods were
in room temperature. Mating ratio in large oviposition room,
in small room,
). But the number of eggs in the large room were
in the small room. As a result of oviposition preference experiment on three different host plants, 10 mated female laid
on the leaves of Phellodendron amurensew more than
on Zanthoxylum schinifolium and
on Citrus unshiu. The egg periods were
days after oviposition, and the hatchability was 92.2% in
room condition. The larval period were
days in the high temperature, long day condition(
, 14L : 10D). The headwidth of each developmental larval stage were
(5th). The pupal ratio was 91.6%. The pupal period were
days, and the emergence rate was 92.2%.
Manufacturing and Characterization evaluation of mulberry concentrate for food additive
Kim, Hyun-Bok ; Seok, Young-Seek ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 180~184
DOI : 10.7852/jses.2013.51.2.180
Study on extraction and concentration of mulberry leaf were performed to increase utilization as new source of food additives. We analyzed extraction method in EtOH, sugar and hot water solution. The desirable method was 70% alcoholic extraction. Color of concentrate was comparatively stable in 70% alcoholic extraction solution and sugar solution. But hot water extraction was showed color change with brown. By filtering of concentrate in 70% alcoholic extraction and sugar extraction, we removed a deposits. Also, we investigated characteristics of the concentrate from mulberry leaf.
Taxonomy of introduced commercial insect, Zophobas atratus (Coleoptera: Tenebrionidae) and a comparison of DNA barcoding with similar tenebrionids, Promethis valgipes and Tenebrio molitor in Korea
Park, Hae Chul ; Jung, Boo Hee ; Han, Taeman ; Lee, Young Bo ; Kim, Seong-Hyun ; Kim, Nam Jeong ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 185~190
DOI : 10.7852/jses.2013.51.2.185
The superworm, as known the larva of Zophobas morio, has been officially imported from 2011 and bred commercially in Korea. But it is named as the corrected scientific name, Zophobas atratus by junior synonym throughout traditional taxonomy in this study and newly designated Korean name as 'a-me-ri-ca-wang-geo-jeo-ri' in terms of resource management. Z. atratus was compared with wild native tenebrionids, Promethis valgipes and a commercial reared Tenebrio molitor on the basis of DNA barcode analysis. As the results, the average genetic divergence was 21.4% between Z. atratus and P. valgipes, and 20.9% between Z. atratus and T. molitor. These large divergences imply these tenebrionids species can be easily identified by DNA barcodes. The results of genetic divergences within species also suggest that Korean populations of Z. atratus, having the same haplotype, might be introduced from the same area of foreign country. On the other hand, a population of T. molitor was separated into two distinct intra-specific groups with DNA barcoding gaps ranged from 1.17- 2.19%. We suppose that domestic breeding entities of T. molitor might be introduced and mixed from two different local groups. Through this study, we expect that classification for two tenebrionid introduced from foreign countries can be used for the management of insect resources in Korea.
Gene expression profile of the early embryonic gene of the silkworm, Bombyx mori
Choi, Kwang-Ho ; Goo, Tae-Won ; Kim, Seong-Ryul ; Kim, Sung-Wan ; Chun, Jae-Buhm ; Park, Seoung-Won ; Kang, Seok-Woo ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 191~196
DOI : 10.7852/jses.2013.51.2.191
This study was aimed for development of a useful genes that has a transcript expressional specificity in the early embryonic stage of the silkworm, Bombyx mori. We constructed and analyzed a full-length cDNA library from silkworm's eggs which after a lapse of 2 ~ 6 hours post oviposit. A total 960 clones were randomly selected, and the 5' ends of the inserts were sequenced to generate 652 expressed sequence tags(EST). 334 unique ESTs were generated after the assembly of 652 ESTs. The annotation of 334 unique ESTs by BLAST search revealed that 156(47%) of the sequences represented known genes, whereas 178(53%) of the sequences has no matches in the database. Of the 156 known genes, the most abundant genes were heat shock protein hsp20.8 gene(12 times) and ubiqutin-like protein gene(11 times). The functional groups of these ESTs with matches in the database were constructed according to their putative molecular functions. Among thirteen functional categories, the largest groups were protein synthesis(9.6%) and cellular organization( 8.1%). Further defined studies on molecular functions and biological roles of their promoters will give us wellfined information and its application.
Transparency of various silk fibroin membranes
Jo, You-Young ; Kweon, HaeYong ; Yeo, Joo-Hong ; Lee, Kwang-Gill ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 197~200
DOI : 10.7852/jses.2013.51.2.197
Silk fibroin is a natural biomaterial that has the biocompatibility and other many advantages. But as a silk fibroin membrane thickness increases, the transparency becomes more opaque. Because the transparency of membranes tissue such as the cornea and dura mater are necessary, transparent membrane is required to replace these transparent membranes. In this study, we fabricated blending silk fibroin membranes that made by mixing the various inorganic salts or polymer in an aqueous solution of silk fibroin. The transparency of the membranes were analyzed. the transparency of these membranes is very different, depending on the mixed materials. Inorganic salts mixed silk membrane was more transparent than the polymer mixed one. Especially, the silk fibroin membrane with calcium chloride was very transparent. We showed the possibility of blending silk fibroin membrane, which can be used in perfect transparent membrane such as the cornea. In the future, we expect that the transparent blending silk fibroin membrane can be used in various medical applications.
DNA barcode analysis for conservation of an endangered species, Aporia crataegi (Lepidoptera, Pieridae) in Korea
Park, Hae Chul ; Han, Taeman ; Kang, Tae Wha ; Yi, Dae-Am ; Kim, Sung-Soo ; Lee, Young Bo ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 201~206
DOI : 10.7852/jses.2013.51.2.201
Aporia crataegi, an Korean endangered species, was first analyzed for DNA barcode sequences based on 28-year-old dried specimens and compared barcode characters with 36 individuals of ten geographical populations of Eurasia. They were revealed to consist of five different haplotypes. Among them, haplotype I was mostly extensive and high frequency with 75%. The south Korean individuals were confirmed to be belonging to haplotype I and have no genetic isolation on COI gene. By these results, we consider that selection of the identical haplotype from other geographical populations may be a requirement prior to performing for conservation and restoration of the Korean population. We also propose to analyse the additional genetic markers in order to understand a more accurate genetic structures between haplotypes of this species.
Effects of matured silkworm hemolymph on suppressing melanin synthesis
Mun, Ji-Young ; Lee, Heui-Sam ; Lee, Kwang-Gill ; Kweon, HaeYong ; Jo, You-Young ; Yeo, Joo-Hong ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 207~210
DOI : 10.7852/jses.2013.51.2.207
In this study, matured silkworm hemolymph was studied for inhibitory effect on melanin formation, tyrosinase activities. A matured silkworm hemolymph mass purification method was developed and some of suppressing melanin formation effects were investigated, too. As a result, the advanced purification method was higher in yield rate than existing one by 53%. A free radical scavenging activity of hemolymph was higher than sericin on the market(
) and lower than vitamin C(
. An inhibitory effect on melanin synthesis was higher in hemolymph compared to Morus bark and arbutin by 9.15% and 11.56%, respectively. An inhibitory effect on tyrosinase activity was higher in hemolymph than Morus bark and lower than arbutin. Effect of hemolymph
on suppressing of tyrosinase was almost 90%. Thus, these results suggest that hemolymph of matured silkworm may have beneficial properties as a material for cosmetics.
Investigation of lifespan related genes of the silkmoth, Bombyx mori L
Choi, Kwang-Ho ; Goo, Tae-Won ; Kim, Seong-Ryul ; Kim, Sung-Wan ; Kang, Seok-Woo ; Kang, Pil-Don ;
Journal of Sericultural and Entomological Science, volume 51, issue 2, 2013, Pages 211~217
DOI : 10.7852/jses.2013.51.2.211
In general, the mean silkmoth lifespan is around 8 days for female and 5 days for male. But, the duration of J037 strain's lifespan is remarkably long in both sexes. On the contrary, the Daizo(sdi) strain has a remarkably short lifetime. The differences in adult lifetime among various silkworm strains has been suggested that the adult lifetime may be genetically controlled. In this experiment, using J037 and Daizo strains we investigated genetic factors related to the adult lifetime of silkworm. We constructed the full-length cDNA library from the adult male of the J037 strain. A total of 2,688 clones were randomly selected, and we performed a differential display hybridization with cDNA probes generated from J037 and Daizo adult males. In conclusion, 193 clones were identified as differential expressed genes, and 154 unique genes were generated after the assembly of 193 clones. Of the 154 unique genes, the most abundant genes were cytochrome oxidase subunit-1 gene(9 times) and unknown(clone ID; 1-50) gene(5 times). The functional groups of these unique genes with matches in the AmiGo database were constructed according to their putative molecular functions. Among thirteen functional categories, the largest group was unclassified protein(24%). In addition, we analyzed the nucleotide and deduced amino acid sequences of the most highly occurred gene(1-50, EF434397), which consisted of 240 amino acids. However, it is confirmed yet that these genes really have an affected on the silkworms longevity. Further studies on these molecules biological roles will give us well-fined information about mechanisms of insect aging and/or scenesence.