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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Sericultural and Entomological Science
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 52, Issue 2 - Oct 2014
Volume 52, Issue 1 - Apr 2014
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Inhibitory effect of melanin synthesis using organic solvent extracts from Scolopendra subspinipes mutilans
Kim, In-Woo ; Lee, Joon Ha ; Kwon, Yong-Nam ; Kim, Sang-Hee ; Yun, Eun-Young ; Nam, Sung-Hee ; Ahn, Mi-Young ; Hwang, Jae-Sam ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 1~5
DOI : 10.7852/jses.2014.52.1.1
This study was performed to determine the whitening effect of organic solvent extracts from the centipede, Scolopendra subspinipes mutilans. We prepared different concentrations (50%, 70% and 100%) of ethanol, methanol, 100% ethyl acetate and water extracts. We tested melanin inhibitory effect and tyrosinase activity using B16/F10 melanoma cell. As a result, treatment of organic solvent extracts is decreased the biosynthesis of melanin and tyrosinase activity to 36 ~ 86%. Especially the 70% ethanol extracts was the most effective in B16/F10 melanoma cells. In the study on melanogenic protein expression, 70% ethanol extracts of Scolopendra subspinipes mutilans blocked glycosylation of tyrosinase. Therefore this result suggests that 70% ethanol extracts could be developed as skin whitening agents.
Stability of main components and physiological activities of bee venom treated with pH
Cho, Miran ; Han, Sangmi ; Kim, Jungmin ; Yeo, Joohong ; Hong, InPhyo ; Woo, Soonok ; Lee, Kwanggill ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 6~9
DOI : 10.7852/jses.2014.52.1.6
This study was for the investigation of the stability of purified bee venom (PBV) during the treatment in the pH range from pH2 to pH9 for 24 hours, respectively. Changes of components and physiological functionalities in PBV were by evaluated silver staining, and melittin contents were measured by liquid chromatography. The antimicrobial activity against bacteria by minimum inhibitory concentration (MIC) and effect of the cell regeneration were measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay using human dermal fibroblast (HDF) cell. The main proteins such as melittin and phospholipase
showed no characteristic changes. The antimicrobial activity and effect of cell regeneration showed no difference from pH2 to pH9. From this study, we suggest that components and physiological functionalities of PBV against treated pH were kept stability at from pH2 to pH9.
Conformational transition of regenerated Antheraea pernyi silk fibroin sponge treated with aqueous ethanol solution and in vitro wound healing effect of wild silk fibroin solution
Lee, Kwang-Gill ; Jo, You-Young ; Yeo, Joo-Hong ; Lee, Heui-Sam ; Kim, Kee-Young ; Kim, Hyun-Bok ; Kim, An-Sook ; Kim, Seong-Gon ; Kweon, HaeYong ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 10~15
DOI : 10.7852/jses.2014.52.1.10
Regenerated Antheraea pernyi silk sponge was prepared using calcium nitrate 4 hydrate melt and examined the conformational changes treated with aqueous ethanol solution. The conformation of silk sponges was changed from random coil structure to
-helix conformation with low ethanol concentration (50 ~ 70%). On the other hand, that of silk sponges with 80% ethanol treatment showed
), and random coil (
) specific peaks. Wound healing effect in vitro was observed by cytoslective wound healing kit. Therefore, regenerated Antheraea pernyi silk sponges might be used as promising wound dressing materials.
A ecological survey of Taraka hamada (Druce) (Lepidoptera, Lycaenidae) in Korea
Lee, YoungBo ; Park, Hae-Chul ; Han, Tae-Man ; Kim, Seong-Hyun ; Kim, Nam-Jung ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 16~24
DOI : 10.7852/jses.2014.52.1.16
Taraka hamada is known as the sole aphidophagous lycaenid butterfly in Korea. The species specifically preys upon Ceratovacuna japonica which lives in bamboo forests. During several years, the species has been received attention from the field of industrial insects in order to commercialize as a pet. However, an ecological character of the species have been unclear in Korea. We carried out a survey for basic ecology and distribution of the two species for 2010 to 2012. As the results, both species only cohabit at Pseudosasa japonica in our investigation. C. japonica is widely distributed from the host plant. Population density of T. hamada is synchronized with the density changes of C. japonica. Their densities are gradually decreased from the point to the investigation and significantly declined in mid-june. The maximum population density of both species shows at the basal part of a leaf. The last-larval instar of T. hamada may seem to be reaching at
stage. The adults of T. hamada are killed within two minutes when they were exposed to the sun.
Expression of the blue fluorescent protein in fibroin H-chain of transgenic silkworm
Kim, Seong Wan ; Yun, Eun Young ; Choi, Kwang-Ho ; Kim, Seong Ryul ; Park, Seung Won ; Kang, Seok Woo ; Goo, Tae Won ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 25~32
DOI : 10.7852/jses.2014.52.1.25
We produced the transgenic silkworm that expressed the enhanced blue fluorescent protein (EBFP) in the cocoon of silkworms. The EBFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EBFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the
-driven DsRed2 cDNA as a marker allowed us to rapidly distinguish transgenic silkworm. A mixture of the donor and helper vector was micro-injected into 300 eggs of silkworms, Baegokjam. We obtained 5 broods. The cocoon displayed blue fluorescence, proving that the fusion protein was present in the cocoon. Also, the presence of fusion proteins in cocoons was demonstrated by SDS-PAGE and western blot analysis. Accordingly, we suggest that the EBFP fluorescence silk will enable the production of the silk-based biomaterials.
Biological control of Frankliniella occidentalis Pergande by Orius laevigatus (Fieber) and new banker plants on chrysanthomum greenhouse
Ham, Eun Hye ; Park, Jong Kyun ; Choi, Yong Seok ; Lee, Jun Seok ; Choi, Young Chul ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 33~38
DOI : 10.7852/jses.2014.52.1.33
This studies by treatment of 25 individual adults of O. laevigatus and Banker plant with different number of eggs of O. laevigatus were conducted to develop new biological control of the western flower thrips (WFT) in chrysanthemum house. In this work, a treatment of 25 individual adults of O. laevigatus, Banker plant with different number of eggs of O. laevigatus and mixed treatment were applied. Initial WFT density per plant were 5 individuals. WFT density was measured every 7days after treatment for 7 weeks. Biological control of WFT by 25 individual adults of O. laevigatus and Banker plant with 25 eggs of O. laevigatus were applied 1 time and resulted in the high control value of thrips 91.8%. The population of WFT was kept under the economic threshold level (1 individual per plant). For the last three years we developed new banker plant system. This system is being developed to improve biological control of WFT on chrysanthemums.
Characterization of the RNA binding protein-1 gene promoter of the silkworm silk grands
Choi, Kwang-Ho ; Kim, Seong-Ryul ; Kim, Sung-Wan ; Goo, Tae-Won ; Kang, Seok-Woo ; Park, Seoung-Won ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 39~44
DOI : 10.7852/jses.2014.52.1.39
We isolated highly-expressed genes in the posterior silk glands of silkworm on a previously study, which one of these was identified as RNA binding protein-1 homologue (RBP-1) gene. In this study, we investigated gene expressional characteristics of the RBP-1 depending on silkworm development stages and several tissues of the larvae, respectively. Northern blot hybridization analysis showed that the RBP-1 gene was expressed high in larval and pupal periods, and highly expressed than endogenous internal control gene (BmA3) on all tested larval tissues. In addition, we isolated and analyzed a phage DNA having 1,660 bp-long promoter region of the RBP-1 gene from a genomic DNA library. To study the RBP-1 gene promoter activity, RBP-1 (-740/+ 30) was amplified by PCR and subcloned into a pGL3 basic vector to generate pGL-RBP1. A luciferase report vector carrying RBP-1 gene promoter (770 bp) was tested by luciferase assay in Sf9 cells. In the result, the RBP-1 gene promoter was more efficient than constitutive promoter (BmA3) by approximately ten percent.
Cloning and functional expression of a cecropin-A gene from the Japanese oak silkworm, Antheraea yamamai
Kim, Seong-Ryul ; Choi, Kwang-Ho ; Kim, Sung-Wan ; Goo, Tae-Won ; Hwang, Jae-Sam ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 45~51
DOI : 10.7852/jses.2014.52.1.45
A cecropin-A gene was isolated from the immunized larvae of the Japanese oak silkworm, Antheraea yamamai and designed Ay-CecA. The complete Ay-CecA cDNA consists of 419 nucleotides with 195 bp open reading frame encoding a 64 amino acid precursor that contains a putative 22-residue signal peptide, a 4-residue propetide and a 37-residue mature peptide with a theoretical mass of 4046.81. The deduced amino acid sequence of the peptide evidenced a significant degree of identity (62 ~ 78% identity) with other lepidopteran cecropins. Like many insect cecropin, Ay-CecA also harbored a glycine residue for C-terminal amidation at the C-end, which suggests potential amidation. To understand this peptide better, we successfully expressed bioactive recombinant Ay-CecA in Escherichia coli that are highly sensitive to the mature peptide. For this, we fused mature Ay-CecA gene with insoluble protein ketosteroid isomerase (KSI) gene to avoid the cell death during induction. The fusion KSI-CecA protein was expressed as inclusion body. The expressed fusion protein was purified by Ni-NTA immobilized metal affinity chromatography (IMAC), and cleaved by cyanogen bromide (CNBr) to release recombinant Ay-CecA. The purified recombinant Ay-CecA showed considerably antibacterial activity against Gram-negative bacteria, E. cori ML 35, Klebsiella pneumonia and Pseudomonas aeruginosa. Our results proved that this peptide with a potent antibacterial activity may play a role in the immune response of Japanese oak silkworm.
Quantitative analysis of rutin with mulberry leaves (I)
Kim, Hyun-Bok ; Kim, Sun-Lim ; Seok, Young-Seek ; Lee, Sun-Ho ; Jo, You-Young ; Kweon, HaeYong ; Lee, Kwang-Gill ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 52~58
DOI : 10.7852/jses.2014.52.1.52
We analyzed rutin content using mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for rutin test. As a result, mean content of mulberry strains was
, and the coefficient of variation (CV) was 47.9%. The variation between the strains was greatly severe. Among the tested strains, `Buyoungsang` was showed the highest content of 1.37%, whereas `Cheongeunsipmunja` and `Mujeonsipmunja` were showed the lowest content of 0.06% respectively. The content of rutin of 16 mulberry varieties for silkworm rearing were compared. `Cheongolppong` was showed the highest content of 0.69%, whereas `Cheongilppong` was showed the lowest content of 0.14 %. Finally we selected rutin high-containing 9 strains. They are as follows. `Buyoungsang`, `Yeulbon`, `Dangsang 5`, `A8`, `Aja`, `Seongsu 5`, `Sawonppong 12`, `Hwanyoupjosaengnosang` and `Hwansipjosaeng` which are more than twice of the overall average content.
Effects of LED on the growth of P. tenuipes
Jo, You-Young ; Kweon, HaeYong ; Lee, Kwang-Gill ; Lee, Heui-Sam ; Yeo, Joo-Hong ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 59~63
DOI : 10.7852/jses.2014.52.1.59
The effects of LED irradiation on growth and functional components of crops are actively being studied. We investigated the effects of red LED on the cordyceps cultivation because Cordyceps is very difficult to find in the wild and to cultivation. The synnemata formation rate of P. tenuipes was 100% under red LED. Also weight, length, and number of the synnemata were significantly increased. We expect that the red LED treatment will be of help to improve farm income.
Breeding of early maturing mulberry cultivar `Suhyang` (Morus alba L.) for mulberry fruit production
Sung, Gyoo Byung ; Kim, Hyun-Bok ; Kang, Pil Don ; Kim, Kee Young ; Ji, Sang Duk ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 64~72
DOI : 10.7852/jses.2014.52.1.64
We bred a mulberry cultivar named Suhyang through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test was carried out at four places (Suwon, Kongju, Wanju and Jinju) for six years from 2007 to 2012. This is belonging to (Morus alba L.) crossed between Josaenghongpisang (Morus alba L.) and Kaeryang (Morus alba L.) among mulberry genetic resources preserved in Suwon. Suhyang was high yielding cultivar in fruit productivity by 12% compared to control cultivar `Chungil (Morus alba L.)` for four years. Although Suhyang was higher in sugar content of mulberry fruits, it was bigger than that of `Chungil`. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.
Establishment of self-specification and shelf-life by standardization of manufacturing process for lyophilized Tenebrio molitor larvae
Chung, Mi Yeon ; Lee, Jeong-Yong ; Lee, Jin-Chae ; Park, Kil-Su ; Jeong, Jun-Pyo ; Hwang, Jae-Sam ; Goo, Tae-Won ; Yun, Eun-Young ;
Journal of Sericultural and Entomological Science, volume 52, issue 1, 2014, Pages 73~78
DOI : 10.7852/jses.2014.52.1.73
This study was carried out to establish the self-specification and shelf-life by standardization of manufacturing process for Tenebrio molitor larvae. First, standardization of manufacturing process for T. molitor was set up. Sterilization for larvae placed on a multistage shelf with intervals of about 10 cm was carried out at
for 10 min. After sterilization, T. molitor larvae were frozen at less than
for more than 12 hrs. And then, they were dried under
, 0.5 torr vacuum for more than 30 hrs. Second, we decided self-specification for T. molitor larvae. Their moisture, acidity, peroxide, crude protein and crude fat level should be 5% or less, 3 mg/g or less, 30 meq/g or less, 45% or more, and 25% or more, respectively. Also, oleic acid, representative material, level was set up 11 ~ 16%. Third, we decided shelf-life by analysis of the physicochemical characteristic, sensory evaluation and microbial indicators. The final expiry date for lyophilized T. molitor larvae in PET bottle was calculated as 12 months from date of manufacture. We expect that optimal manufacturing process system, self-specification, and shelf-life proposed in this study can be used in industrial production of T. molitor as a novel food.