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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 10, Issue 5 - Nov 1995
Volume 10, Issue 4 - Oct 1995
Volume 10, Issue 3 - Jul 1995
Volume 10, Issue 2 - May 1995
Volume 10, Issue 1 - Mar 1995
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Production of Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid by Pseudomonas sp. HJ
KSBB Journal, volume 10, issue 4, 1995, Pages 349~356
To produce PHA(polyhydroxyalkanoic acid) from microbr, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source (glucose) that was not generally considered as precursor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were
and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH4) 2SO4 0.2% as a nitrogen source, K2HPO4 0.3%, and KH2PO4 0.45%. TO investigate she optimal condition for PHA production two-step cultivation method was employed. PHA production was inducted by deficiency of NH4+, SO4-2, Mg+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH4+ stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to pyoduc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. The contents of PHA and she proportions of hydroxyvalerate monomer units varied depending on the carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by HNMR analysis.
Mathematical Model for Adsorption of Berberine on Encapsulated Adsorbent
KSBB Journal, volume 10, issue 4, 1995, Pages 358~369
A mathematical model using local thermodynamic equilibrium isotherms for adsorption in encapsulated adsorbent is proposed in order to optimize the design parameters in situ bioproduct separation process. The model accurately follows the experimental data on the adsorption of berberine, secondary metabolite produced in Thaictrum rugosum plant cell culture. The adsorption rate on encapsulated adsorbent is compared with that on alginate-entrapped adsorbent. The result shows that the higher loading capacity in encapsulated adsorbent is mainly due to the increase in the maximum solid phase concentration. Based on the adsorption rate and loading capacity, the encapsulated adsorbent would be more useful than the entrapped adsorbent when used in situ bioproduct separation process. Design parameters in situ bioproduct separation process, such as the size of the capsule, membrane thickness, the ratio of capsule volume to bulk volume, the ratio of single capsule volume to total capsule volume and the adsorbent content in the capsule, are evaluated by using the model. The ratio of single capsule volume to total capsule volume is the most effective parameter for adsorption of berberine on encapsulated adsorbent.
Improvement of Ethanol Yield by Addition of Acetic Acid and Acetatdehyde in Ethanol Fermentation
KSBB Journal, volume 10, issue 4, 1995, Pages 370~373
The major by-products in ethanol fermentation by Saccharomyces cerevisiae were glycerol, acetaldehyde, acetic acid, lactic acid, and formic acid. The effects of these by-products on the cell growth and ethanol production were studied. By adding acetaldehyde or acetic acid in the fermentation broth, the cell growth decreased while the ethanol production increased. But glycerol and lactic acid had nearly no effects on the cell growth and the ethanol production. Acetic acid and acetaldehyde inhibited the cell growth by diminishing the growth rate as well as by prolonging the lag phase. The ethanol yield increased with the elevation of concentrations of acetic acid and acetaldehyde in the fermentation broth. The maximum ethanol yield was obtained for
acetic acid and
A Study on the Optimum Conditions of Gelatin-Degrading Proteolytic Enzyme Production from Bacillus subtilis B0021
KSBB Journal, volume 10, issue 4, 1995, Pages 374~385
Nutritional requirements and cultural conditions for the production of extracellular gelatin-degrading proteolytic enzyme by Bacillus subtilis B0021 were investigated. Optimum carbon source for proteolytic enzyme production was salicin, but it was substituted by glucose for economical reason. The fermentation medium giving a maximum proteolytic enzyme activity was consisted of 1.5%(w/v) glucose, 2.5%(w/v) yeast extract, and 0.001%(w/v) manganese sulfate and 0.002%(w/v) ferrous sulfate. Proteolytic enzyme activity of B. subtilis B0021 was completely inhibited by 0.5%(w/v) tannic acid. Initial pH was optimal at 7.0 and the enzyme activity in the flask culture usually reached a maximal level after 36 hours of fermentation at
. In the
fermentor fermentation at
, enzyme activity was maximum at 36 hour of cultivation but after this enzyme activity was decreased rapidly. Initial viscosity of 45%(w/v) gelatin(2,800mPas) was decreased rapidly to 96%(mPas) after hydrolysis for 4hr at
by crude enzyme of B. subtilis B0021.
Solution Properties of
-Polyglutamic Acid Produced by Alkalophilic Alcaligenes sp.
KSBB Journal, volume 10, issue 4, 1995, Pages 386~392
The high viscous
-PGA) from alkalophilic Alcaligenes sp. was purified and its solution property was investigated. The intrinsic viscosities for Na+ form and H+ form of
-PGA were 31.1 and 0.38d
/g, respectively. The viscosity of H+-PGA was not influenced by pH or salts while that of Na+-PGA was influenced. The intrinsic viscosity of Na+-PGA solution decreased remarkably at the alkaline or acidic pH and showed the sharp decrease when salts were added.
-PGA exhibited the property of the polyelectrolyte showing the .sharp decrease of intrinsic viscosity by the addition of NaCl, and intrinsic viscosity of dilute solution with the low concentration of NaCl was exponentially dependent on temperature and its temperature dependency increased with increasing NaCl concentration. The chain stiffness, coil overlap parameter and critical concentration of Na+-PGA were 0.08, 5.25 and 0.07g/d
Studies on the Distribution of Dendropanax morbifere and Component Analysis of the Golden Lacquer
KSBB Journal, volume 10, issue 4, 1995, Pages 393~400
As a part of the .study on the multiple propagation and use of Dendropanax mcrbifera, distribution of this tree and component analysis of Golden Lacquer were investigated to reappear the Golden Lacquer, traditional paints. These results were summarized as follows. The natural growth habitat of Dendropanax morbifera was observed in 8 sites(Wando, Sanghwangbong, etc.), we could be found that Duryun nountain in Haenam for the first time. Dendropunax morbifera has grown from 100 to 450m above the sea level, appeared high frequency in the 200m. The soil pH of natural growth site was pH 4.9 to pH 5.8. Dendropanax morbifera grew in the area containing considerable moisture in that its contents was 16.5% to 27.4%. In community observation, dominance and community index of Bogildo and Chejudo was 3.3 in the forest tree layer and Wando was showed in the sub-forest tree layer mainly. In the growth conditions, Bogildo appeared the highest growth state that diameter of height of human chest was 1.0 to 20cm and tree height was 0.2 to 9m. As result of IR and GC-MS analysis, main component of Golden Lacquer was
-selinene and capnellane-8-one.
Kinetics of Cultivating Photosynthetic Microalga, Spirulina platensis in an Outdoor Photobioreactor
KSBB Journal, volume 10, issue 4, 1995, Pages 401~405
An open pond type photobioreactor for mass cultivation of S. platensis was designed and the growth parameters from different cultivation processes were compared. 0.30(1/day) of specific growth rate and 1.69(g/
) of maximum cell density were obtained from batch cultivation. In fed-batch cultivation, specific growth rate and maximum cell density were estimated as 0.22(1/day) and 1.75(g/
), respectively. Maximum biomass productively from continuous cultivation was obtained as 0.44 (g/
/day). It proves that an outdoors-mass cultivation of S. platensis considering optimal environmental condition is economically feasible. In addition, the biomass productivity was studied in two different mixing systems such as agitation and air sparging methods. The biomass productivity by an agitation method was better than that in an air sparging method.
Production and Application of Polyhydroxyalkanoates
KSBB Journal, volume 10, issue 4, 1995, Pages 406~420
Polyhydroxyalkanoates[PHAs] are the polyester of hydroxyalkanoates(HAs) synthesized by numerous bacteria as an intracellular carbon and energy storage compound and accumulated as granules in the cytoplasm of the cells under unbalanced growth condition in the presence of excess carbon source. Even though PHAs have been recognized as good candidates for biodegradable plastics, their high price compared with conventional plastics has limited their use in a wide mange of applications. To reduce the high production cost of PHAs, many group of scientists have devoted much efforts to improve productivity by employing various microorganisms and by developing efficient culture techniques. The strategies of producing PHAs to a high concentration with high productivity and their potential applications are reviewed.
Isolation of Microorganism Producing Flocculant and Its Culture Conditions
KSBB Journal, volume 10, issue 4, 1995, Pages 421~427
A fungal strain, designated Aspevgillus sp. JS-42, was isolated and shown to produce an extracellular polysaccharide used as a bioflocsulant. The optimal medium composition for the production of flocculant with Aspergillus sp. JS-42 was glucose 3.0%, yeast extract 0.2%,
in distilled water. The optimum culture temperature and optimum culture pH for the production of the flocculant were
and pH 7.0, respectively. The highest production of flocculant was observed after 90 hours of cultivation at the optimal condition. The flocculant could efficiently flocculaled the tested solids suspended in aqueous solution and was stable at high temperature(
) and to pH range of from 2 to 10. The flocculant seems to be a kind of high viscous polysaccharide.
Preparation and Destabilization of Target-Sensitive Liposomes
KSBB Journal, volume 10, issue 4, 1995, Pages 428~434
Target-sensitive(TG-S) liposomes, which have the antibodies coupled on the surface of liposome and can release their entrapped contents by the binding of antibodies with the specigic target cells, were prepared and employed to study the release of calcein and the selective delivery of an anticancer agent, doxorubicin(DOX). The monoclonal antibody, Y3, used for the preparation of the TG-S liposome was one against major histocompatibility complex class 1 of mouse(MHCI, H-2Kｂtype) and the target cells were EL-4 and RMA, which have the MHC1, H-2Kbtype on their membrane surfacem. The release of calcein from TG-S liposome occurred when the target cells were contacted with liposomes and it was proportionally increased with the rise of binding capacity of antibody coupled on the surface of liposome to the target cells. The experimental results of drug delivery were similar to the cases of calcein release. The viability of specific target cell, EL-4 with liposomal DOX was not so different from that with the free DOX, while for the non-specific target cell, Yacl(H-2Kf), the cell viability with Iiposomal DOX was much higher than that with free DOX. This shows the fact that the liposomal DOX can be efficiently delivered to the specific target cells, while it was not the case for the non-specific target cells. And the drug delivery was lnhibited when the free antibody of Y3 was added in the contact process between EL-4 and TG-S liposomes, which means the drug delivery occurred mainly by the destabilization of TG-S liposomes. From these results, we could conclude that the selective drug delivery to specific target cell using the TG-S liposome would be feasible.
Effect of Polyamine on Hairy Root Culture of Bupleurum falcatum L.
KSBB Journal, volume 10, issue 4, 1995, Pages 435~440
During culture the effect of polyamine on hairy root of Buplerum falcatum by infection of Agrobacterium rhizogenes was studied. The fresh and dry weight of hairy root which cultured for 3months in MS medium increased about 7-fold when spermine at 10
M was treated. After suspension culture of B. falcatum in MS medium containing putrescine(10
M) or spermine(10
M), the contents of endogenous polyamine (putresclne, spermidine, spermine) was higher than that of control. The
-glucan synthetase II activity by polyamine treatment was increased： especially spermidine (100
M) and spermine(100
M) stimulated it by about 180% and 220% respectively. These results suggest a possible role of polyamine as growth regulator in B. falcatum hairy root cultures.
Cultural Conditions of Exopolysaccharide KS-1 Produced by Bacillus polymyxa KS-1
KSBB Journal, volume 10, issue 4, 1995, Pages 441~448
Optimized fermentation medium and cultural conditions for the production or exopolysaccharide KS-1 with Bacillus polymyxa KS-1 was following as; 30g g1ucose, 2.59g yeast extract, $2.5g KH_2PO_4, 0.5g NaCl, 0.3g MgSO_4.7H_20, 0.1g CaC0_3 0.05g, FeSO_4.7H_2O, and 0.05g MnS0_4 . 4H_20in 1 liter distilled water. The exopolysaccharide production was influenced by the by the temperature and pH, the optimal conditions for the production of exopolysaccharide KS-1 seemed to be
and pH 7.0, respectively. About
of maximum exopolysaccharide was obtained al the initial pH 7.0,
, 2vvm of aeration rate and 400 rpm of impeller speed in a jar fermentor.
The Effects of Metathesis for Concentrating the Tocopherols from Soybean and Rice-bran Scum Oils
KSBB Journal, volume 10, issue 4, 1995, Pages 449~454
The effects of metathesis for concentrating the tocopherols from soybean and rice-bran scum oils were studied by using the batch reactor under helium atmosphere. The contents of tocopherols in the scum oils decreased consticuously when heated under air atmosphere or when kept in hexane solution above 5 days even at room temperature. The sterols in the scum oils were removed by the mixed solvent method. Metathesis of the sterol-removed scum oils in hexane was performed over Re2O7/Al2O3 and WO3/Al2O3 catalysts, and the concentrate was obtained by distillation in vacuum at
. The effect of metathesis was evaluated as relative ratio of
-tocopherol in the concentrate to that in scum oil. The maximum ratio for both scum oils was obtained on 12.8%(w/w)
catalyst which formed effectively the active sites for metathesis by the reaction between the added tetramethyltin and
on the surface of the catalyst. The optimum amount of the catalyst was 0.5g pre l0g scum oil, and the optimum reaction temperature was
for both scum oils. The metathesis was more effective in rice-bran scum oil than in soybean scum oil. These facts indicated that the tocopherols in the scum oils can be highly concentrated by applying metathesis.
Effect of Acetic Acid Formation and Specific Growth Rate on Productivity of Recombinant Escherichia coli Fed-Batch Fermentation
KSBB Journal, volume 10, issue 4, 1995, Pages 455~460
Specific growth rate was controlled for the repression of acetic acid formation in the fed-batch fermentation of recombinant Escherichia coli. With controlled specific growth rate, we studied the effect of the specific growth rate on cell growth, glucose consumption, acetic acid formation, and the expression of recombinant protein (
-lactamase). High specific growth rate caused the accumulation of glucose and acetic acid, and lowered the production of recombinant protein. However, the addition of methionine recovered the gene expression by alleviating the negative effect of acetic acid at high specific growth rate.
Drug Release by Poly(DL-lactide) Coated Chitosan Derivatives Matrices
KSBB Journal, volume 10, issue 4, 1995, Pages 461~467
In this study, the release experiments of drug were operated in the phosphate buffer solutions of pH 7.4 and pH 1.2 by using drug carriers(chitosan, chitosan hydrochloride, and sulfonated chitosan)coated by poly(DL-lactide) with prednisolone for delivery drug. The release time of drug was more delayed in pH 7.4 than in pH 1.2. The release time of according to the kinds of drug carrier was delayed in the order of chitosan, sulfonated chitosan, and chitosan hydrochloride. In short, the formulation allows biodegradable coated monolithic polymetic matrices to suppress the burst effect of the drug release mechanism, which led to the sustained release pattern.
Study of Enzyme Immobilization on Composite of CTA and PCL Membrane for Biosensor
KSBB Journal, volume 10, issue 4, 1995, Pages 468~474
The disposable glucose bio-sensor using composite of CTA and PCL membrane was developed for measurement of glucose. The most effective membrane was composed of CTA/PCL(80/20, w/w) and glutaraldehyde one-step immobilization method (
thickness) for glucose sensor gave the best result among various methods, considering oxygen permeability and electronic sensitivity. A scanning electron micrograph of the cross-section of a typical asymmetric CTA/PCL composite membrane showed that the membrane fused with a dense layer covered with a GOD-glutaraldehyde. Glucose oxidase immoblilized on the membrane showed the linearity between difference of absolute amperometric values and glucose concentrations within 7mM when the GOD immobilized electrode was used. About 35% of activity was remained after 8 days when the tyrosinase was immobilized on CTA/PCL (80/20) membrane.