Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 11, Issue 6 - Dec 1996
Volume 11, Issue 5 - Oct 1996
Volume 11, Issue 4 - Aug 1996
Volume 11, Issue 3 - Jun 1996
Volume 11, Issue 2 - Apr 1996
Volume 11, Issue 1 - Feb 1996
Selecting the target year
A Study on Alkyl Glucoside Synthesis by Amphiphilic Phase Enzyme Reaction
KSBB Journal, volume 11, issue 5, 1996, Pages 511~517
An amphiphilic phase enzyme reaction was used to synthesize alkyl glucosides from glucose and alkyl alcohol with immobilized
-glucosidase using four glycol ether cosolvents(monoglyme, diglyme, 2-methoxyethanol, and 1,4-dioxane). Monoglyme was shown to be the best cosolvent for the amphiphilic phase medium composed of water/cosolvent/alkyl alcohol admixture. To obtain high yield of alkyl glucoside by amphiphilic phase enzyme reaction, hydrophilicity-hydrophobicity of amphiphilic media and enzyme microenvironment was optimized from the viewpoints of substrate solubility, enzyme activity, water activity, and dynamic equilibrium between glucose alcoholysis and glucoside hydrolysis. Under optimum reaction conditions, the highest concentrations of hexyl, octyl, decyl, and dodecyl glucosides were 18.2, 9.68, 7.27, and 6.03g/L, respectively.
ATPase activities in Fish Muscle Protein by ATPase Biosensor
KSBB Journal, volume 11, issue 5, 1996, Pages 518~523
The sensor to determine ATPase activities was consisted of an immobilized enzyme membrane(purine nucloside phosphoryrase and xanthine oxidase) and an oxygen electrode. The proposed sensor was used for the determination of
ATPase activities in several fish muscle proteins such as Thunnus albacares(Yellowfin tuna), Tetrapturus audax(Striped marlin), Prognichthys agoo(Japanese flyingfish), and Cypvinus carpio(Carp).
ATPase activities measured by the proposed sensor system were in good agreement with the results obtained by a conventional colorimetric assay. One cycle of assay could be completed within 3mlnutes.
Degradation of a Pesticide, 4-Chloro-2-methylphenoxyacetic Acid by Immobilized Biofilm in Bench-scale Column Reactors
KSBB Journal, volume 11, issue 5, 1996, Pages 524~528
Bacterial degradation of 4-chloro-2-methylphenoxyacetic acid (MCPA) was studied in column reactors under conditions approximating a fluidized bed system, with granular activated carbon (GAC) as a support matrix. A mixed bacterial culture of MCPA-degrading bacteria was used as an inoculum to develop a biofilm on GAC. Initially, adsorption of MCPA by GAC and blofilm formation on GAC were examined. MCPA degradation was evaluated with a batch and continuous mode of operation of the GAC fixed-film column reactors. In the batch operations, complete degradation of MCPA was achieved during the incubation period. Partial degradation of MCPA occurred in the continuous operations and MCPA degradation was dependent on the feeding rate of MCPA solution.
A Study on the Purification Process of Methyl Fructoside by Liquid Chromatography
KSBB Journal, volume 11, issue 5, 1996, Pages 529~535
Methyl frucloside was purified from the aqueous sugar/methyl fructoside solution by liquid chromatography using Amberlite IRA-900, strong anion-exchange resin. The optimum operating conditions, resolution and productivity of methyl fructoside were discussed to evaluate the practical feasibility of the proposed chromatographic separation process of methyl fructoside which is useful as a new starting material for sugar ester synthesis. The linear chromatography model with HETP was well applied to the chromatographic separation process of methyl fructoside and the theoretical solution successfully predicted the elution chromatogram of methyl fructoside and sucrose at different superficial linear velocity of eluent for rectangular feed with different loading volume of packed bed. The optimum operating conditions were found to be 75% with the loading volume of packed bed at 1.13 cm/min of the superficial linear velocity at
, and gave the productivity of methyl fructoside of 7 mg/g-resin/h with the resolution of 1.1.
Purification and Structure Determination of the GTase Inhibitor from Cacao Bean Husk Extract
KSBB Journal, volume 11, issue 5, 1996, Pages 536~542
The isolation of active compounds showing the inhibitory effect on glucosyltransferase(GTase) from cacao bean husk(CBH) extract was carried out for screening of anti-plaque agents. These active compounds were purified by additional column chromatography of MCI-gel CHP-20 and Sephadex LH-20 and their chemical structures were determined by NMR and mass spectroscopy. Two compounds showing the inhibitory effect on GTase from CBH extract were obtained. These compounds showed positive reactions with anisaldehyde-H2SO4 solution and FeCl3, and were identified as dimeric flavan-3-ols on TLC. By NMR and MS data analyses, the structures of two different flavan-3-ols were identified as procyanidin B-1 and procyanidin B-3, respectively.
Growth and Cadmium Removal in Recombinant Saccharomyces cerevisiae Harboring A Metallothionein Gene
KSBB Journal, volume 11, issue 5, 1996, Pages 543~549
Recombinant Saccharomyces cerevisiae BZ-pJ containing the gene coding for metallothionein, a metalbinding protein was grown in the medium with high cadmium concentrations to study the characteristics of growth and cadmium uptake. High concentrations of cadmium reduced cell growth and final cell density and increased the lag phase periods of the recombinant yeast. Addition of 10 mg
/L to the growth medium remarkably decreased a lag period and enhanced the specific cadmium uptake to 52.6 mg
/g dry cell. The effect of copper addition was further investigated in the medium of 680 mg Cd2+/L. An increase in copper concentration from 11.0 to 33.3 mg/L enhanced the specific cadmium uptake from 17.0 to 42.0 mg Cd2+/g dry cell.
The Optimal Medium Composition for the Production of Microbial Cellulose by Acetobacter xylinum
KSBB Journal, volume 11, issue 5, 1996, Pages 550~556
A complex medium was developed for the production of microbial cellulose by Acetobacter xylinum ATCC 23769. The optimum concentration of each nutrient for the production of microbial cellulose was determined to be 10g peptone, 20g yeast extract, 5g glucose, 1.56g Na2HPO4, 1.8g KH2PO4, 0.05g MgSO4, 0.002g FeCl3, 5g citric acid and 10 mL ethanol per liter. With synergistic effects of citric acid and ethanol, cellulose productivity achieved in developed medium was 0.446 gram of cellulose per gram glucose for static culture, which is much higher than reported values. Cell growth and the cellulose production in the developed medium under static culture was also investigated.
FT-IR and Raman Spectroscopy for the Interaction between Poly(2-hydroxyethylmethacrylate) and Amino Acids
KSBB Journal, volume 11, issue 5, 1996, Pages 557~564
The interaction between poly(2-hydroxyethylmethacrylate)(poly(HEMA)) which is a material of contact lens containing approximately 45%o water and water soluble amino acids (alanine, arginine, glycine, lysine, methionine, proline, and serine) was investigated by using FT-IR and Raman spectroscopy. The results revealed that arginine and lysine had the strongest interaction with poly(HEMA) among amino acids. The interaction depended on the quantity of charges on ammo acids. They interacted predominately with hydroxyl groups in poly(HEMA).
Optimization of Medium for
-Mannanase Production by Aspergillus oryzae
KSBB Journal, volume 11, issue 5, 1996, Pages 565~571
Medium optimization for
-mannanase production by Aspergillus oryzae ATCC 2114 was performed. Effect of carbon source (locust bean gum) concentration on
-mannanase production was investigated. Above 20 g/L locust bean gum, a lag time for
-mannanase production was appeared because high concentration of locust bean gum caused high viscosity which made the mixing of medium poor. As the locust bean gum concentration in the medium increased,
-mannanase activity and cell growth increased proportionally. Effect of various nitrogen sources on
-mannanase production was also studied. (NH4)2SO4 and malt extract were the most effective for
-mannanase production among the inorganic nitrogenous compounds and organic nitrogen nutrients. Inorganic compounds such as KH2SO4, NaCl, Na2CO3, and MgSO4, on
-mannanase production were optimized for
-mannanase production. Locust bean gum of 10 g/L, malt extract of 3 g/L, (NH4)2SO4 of 2 g/L, KH2SO4, of 10 g/L were selected as the optimal medium. Culture in a fermentor by using the optimal medium was carried out. Lag time of
-mannanase production was shorter due to the better mixing of the fermentor. The maximum
- mannanase activity of 9.7 unit/mL and specific
-mannanase activity of 1.9 unit/mg-cell could be obtained at 27 hours and the productivity of
-mannanase was 0.36 unit/mL
Production of Pleurotus spp. Mycelium Using Rancid Frying Oils
KSBB Journal, volume 11, issue 5, 1996, Pages 572~576
Conditions for the culture of Pleurotus spp. mycelium using rancid frying oils were investigated. Among the six strains tested, Pleurotus ostreatus CBS 03 showed the greatest mycelial growth on fish paste and ramyon frying oil, and was used in this study. The optimum temperature and pH for mycelial growth were from 25 to
and pH 5.5 to 6.0, respectively. Tryptone for mycelial growth was better than any other nitrogen sources. The addition of
enhanced mycelial growth at 0.2 and 0.01% on fish frying oil, and at 0.1 and 0.03% on ramyon frying oil. Among the vitamins used, thiamine and nicotinic acid were the most effective ones.
Degradation of Phenol by Activated Sludge Immobilized with Photo-crosslinked Resin
KSBB Journal, volume 11, issue 5, 1996, Pages 577~585
Effects of various factors on the phenol degradation by activated sludge immobilized with the photo-crosslinked resin were investigated. The optimum pH on the degradation of phenol in both free and immobilized activated sludge was 7. When the pH of the reaction was varied from 5 to 10, the relative activity of the phenol degradation by the immobilized activated sludge was higher than that by the free activated sludge. A higher rate of phenol degradation was observed when a bead size was smaller. The phenol degradation in the free activated sludge was inhibited at the 3000 mg/L of phenol, while that in the immobilized activated sludge was maintained at the same concentration for 28 hrs without an inhibition. The degradation rates of phenol were not directly proportional to the increasing amount of immobilized beads dosage, but the phenol degradation was made in a rather short time than that for a free sludge system. The relative activities of the immobilized activated sludge after 7 runs of repeated reactions increased about 8 times as that of the first reaction. The activities for the phenol degradation remained stable for at least 80 days when the immobilized activated sludge was stored at an aerobic condition in the wastewater containing phenol. The loading rate as high as 5.59 kg-pheno1/㎥.d could have been achieved during the continuous treatment of phenol by the immobilized activated sludge.
Effect of Cosubstrate on tile Production of Poly(3-Hydroxybutyric-Co-3-Hydroxyvaleric) Acid from Glucose by Pseudomonas sp, HJ
KSBB Journal, volume 11, issue 5, 1996, Pages 586~592
Poly(3-hydroxybutyric-co-3-hydroxyvaleric) acid(PHB/HV) copolymer synthesis by Pseudomonas sp. HJ from glucose and cosubstrate was investigated. Taxonomic analysis suggested that Pseudomonas sp. HJ was best marched to Pseudomonas picketti having 78.8% similarity. Pseudomonas sp. HJ produced PHB/HV copolymer containing 60.8 mol% HV and 44.9 mol% HV when supplied with hexadecane and propionic acid as a cosubstrate, respectively. The HV composition in PHB/HV copolymer was controlled by varying the concentration of hexadecane and propionic acid. Propionic acid added after 24 hours of incubation was incorporated as the HV monomer in the PHB/HV copolymer up to 49.6 mol%.
Isolation of Agar Degrading Bacteria, Cytophaga sp. ACLJ-18 and Optimization of Enzyme Production
KSBB Journal, volume 11, issue 5, 1996, Pages 593~599
The strain which produces agar degrading enzyme was isolated from chiton(Liolophura japonica). The strain was identified as Cytophaga sp. through its morphological, physiological, and biological characteristics. For the production of agar degrading enzyme, 0.3% nutrient broth, 0.2% yeast extract and 0.5% agar was used as nitrogen and carbon source, respectively. The optimal initial pH, NaCl and temperature for the agar degrading activity of Cytophaga sp. were 7.0, 2.0% and
, respectively. Agar degrading activity of enzyme obtained from Cytophaga sp. was increased until the incubation of 96hrs, but after 96hrs, the activity was decreased.
Effects of Inoculum Density and Basal Media on Cell Growth and Taxol Production in Taxus Cell Suspension Cultures
KSBB Journal, volume 11, issue 5, 1996, Pages 600~605
Optimum inoculum concentration for the production of taxol was determined in Taxus brevifolia and Taxus cuspidata cell suspension cultures. By fresh weight, 2.5, 5, 7.5, 10 g/flask of cells were inoculated and cell growth as well as taxol production were examined. In both Taxus cell cultures, the higher the inoculum concentration, the shorter the length of the lag period. The optimum inoculum concentration for taxol production was found to be 5 g/flask. To produce taxol in large quantity, utilization of proper medium was thought to be important. In case of using a production medium with 6% sucrose, taxol production was noticed. Its level reached the maximum at the 9th day of culture and decreased afterwards. However, taxol was not detected from cell cultures in growth medium.
Ion Exchange of Glutamic Acid Coupled with Crystallization
KSBB Journal, volume 11, issue 5, 1996, Pages 606~612
A specific ammino auid in a mixture can be crystallized inside an ion exchange column when displacer concentration is high enough to concentrate the amino acid in a pure band beyond its solubility limit. Glutamic acid formpd a discrete crystal layer in a cation exchanger column by operating displacement development mode and using a high concentration of displacer NaOH. The glutamic acid crystal formed was eluded from the column with the effluent stream and collected in a fraction collector. When 1.0 M of NaOH was used as a displacer, more than 60% of the loaded glutamic acid was recovered as crystal. The continuous crystallization and dissolution of crystal occurred, resulting in apparent movement of the crystal along the column without clogging or pressure increase. NaOH was proved a better displacer than NaCl because hydroxide ions neutralized hydrogen ions released from the resin and thus reduced the number of hydrogen ion competing with sodium ion for re-adsorption. The displacement development process coupled with crystallization provided higher concentration and recovery of glutamic acrid than conventional chromatography.
Effect of Medium Components on the Production of Cyclosporin A by Immobilized Fungal Cell, Tolypocladium inflatum
KSBB Journal, volume 11, issue 5, 1996, Pages 613~621
The effects of important medium components such as carbon, nitrogen sources and amino acids on the production of cyclosporin A(CyA) were investigated in an immobilized fungal cell fermentation using Tolypocladium inflatum. As carbon sources in the synthetic medium, fructose and maltose stimulated CyA production remarkably compared to glucose when ammonium sulfate was supplemented as a nitrogen source. In the absence of ammonium sulfate in the medium, however, CyA biosynthesis was reduced considerably without regard to C-sources tested. Ammonium sulfate was found to be the best N-source, and also ammonium phosphate and ammonium citrate showed some positive effects on CyA production. Optimum concentration of ammonium sulfate was 10g/L, and supplementation of ammonium sulfate at the start of fermentation was found to be the most efficacious for maximal production of CyA. Among the constituent amino acids of cyclic peptide, CyA, L-valine had the most significant effect on the biosynthesis of CyA, and maximum CyA production was observed when 10 g/L of L-valine was initially added.