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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 14, Issue 6 - Dec 1999
Volume 14, Issue 5 - Oct 1999
Volume 14, Issue 4 - Aug 1999
Volume 14, Issue 3 - Jun 1999
Volume 14, Issue 2 - Apr 1999
Volume 14, Issue 1 - Feb 1999
Volume 14, Issue 5 - 00 1999
Volume 14, Issue 4 - 00 1999
Selecting the target year
Stepwise Addition Technology for the High Yield Production of Chitosanoligosaccharide
Lee, Ki-Sun ; Kim, Seung-Mo ; Lim, Hyun-Soo ;
KSBB Journal, volume 14, issue 6, 1999, Pages 639~642
Optimization of the chitosanoligosaccharide production was studied with chitosanase. The optimum conditions for the enzymic reaction have been determined. Enzyme stability was maintained above 90% after 6 days at pH 5.0. The optimum initial reaction rate was appeared in 1.0% of chitosan solution. The production yield of chitosanoligosaccharides was over at 0.4%~2.0% of chitosan. At 4.0% of chitosan solution, however, the production yield was decreased to 64.6%. To increase the yield, stepwise addition of substrate into the reactor was investigated. In this case, the yield was increased from 64.6% to 83.2% and the final concentrations of chitosanoligosaccharide was 12.26 mg/mL. By TLC analysis, most of the chitosanoligosaccharides produced were 3-5 mers.
Bacteriorhodopsin/Flavin Complex LB Films-Based Artificial Photoreceptor for Color Recognition
Choi, Hyun-Goo ; Jung, Woo-Chul ; Min, Jun-Hong ; Lee, Won-Hong ; Choi, Jeong-Woo ;
KSBB Journal, volume 14, issue 6, 1999, Pages 643~650
An artificial photoreceptor composed of bacteriorhodopsin(bR)/flavin complex Langmuir-Blodgett(LB) films was developed by mimicking the human visual system. bR and flavin molecules were deposited onto solid substrate by LB technique, and the deposition of two molecules was proved by UV/VIS absorption spectroscopy and atomic force microscopy(AFM). Based on AFM images and photocurrent generation from the LB films, the optimal conditions for device fabrication were determined. With a series of light illuminations, the generated photocurrent could be detected, and the response characteristics of two molecules could be clearly distinguished from each other. According to the obtained signal shapes, three distinctive regions could be found in the obtained action spectrum. Using a correlation between the photocurrent generation and the wavelength of the input light, it was possible to organize the basic rules to interpret the wavelength of the input light. It is concluded that the proposed artificial photoreceptor would e applicable to the bioelectronic device for color recognition.
Monitoring of Structural Changes during in vitro Unfolding and Refolding of Recombinant Human Growth Hormone
Cho, Tae-Hoon ; Chai, Young-Kyu ; Ahn, Sang-Jeom ; Lee, Eun-Kyu ;
KSBB Journal, volume 14, issue 6, 1999, Pages 651~654
Using recombinant human growth hormone as a model protein, we carried out unfolding by adding a denaturant such as urea, guanidine HCl, or SDS followed by refolding by dilution and dialysis. The objectives were to monitor the structural changes during in vitro refolding process and, based on the results, to develop a quantitative method of refolding progress assessment. The changes in surface hydrophobicity were measured by fluorescence tagging of 1-anilinonaphthalene-8-sulfonate(1,8-ANS) to the hydrophobic portions, and those in the secondary structure were monitored by using far UV-CD(circular dichroism) spectroscopy. Also, we used RP-HPLC to separate and quantify the folded and unfolded proteins to correlate the result with the structure analysis. Our results indicate the surface hydrophobicity are well correlated with the formations of the secondary structure, primarily
-helices, as well as the disulfide bridges. We expect this monitoring technique can be applied in industrial fields as a means to quantitatively assess the progress of in-vitro refolding of recombinant proteins.
Fractional Extraction of DHA and EPA by Supercritical Fluid
Hur, Byung-Ki ; Noh, Duck-Woo ;
KSBB Journal, volume 14, issue 6, 1999, Pages 655~660
Supercritical fractional extraction of DHA and EPA from the mixture of fatty acids composing the fish oil was performed. The compositions, extracted quantities, and cumulative extracted quantities of fatty acids in the extract and the raffinate were investigated according to the fractional steps. The temperature and pressure for the miximum concentration of DHA and EPA in the extract or the raffinate were 60
and 101 bar respectively among the extraction conditions considered in this study. In this case, the weight percent of EPA in the extract was 50% and that of DHA in the raffinate was 40%. These values were two times higher than those in fish oil. THe same temperature and pressure were used to extract DHA and EPA from the mixture of fatty acids whose the initial weight percent of DHA was 34%. The weight percent of DHA in the raffinate after the fourth fractionation was 7-%. And the remaining weight of DHA in the raffinate was 80% of DHA initially loaded in the extraction vessel.
Optimum Conditions of Lysozyme Extraction Using Reversed Micelles
Chun, Byung-Soo ; Kim, Suk-Kyoo ; Yoon, Sung-Ok ; Song, Seung-Koo ;
KSBB Journal, volume 14, issue 6, 1999, Pages 661~664
Proteins were extracted from an aqueous phase with reversed micelles. The effect of pH, and salt concentration on the solubilization of lysozyme in AOT/isooctane solution was studied to explore the potential for employing this solvent system in the large-scale recovery and concentration of proteins using liquid extraction. For pH values below the isoelectric point, pl of the protein, solubilization was high, probably owing to strong electrostatic interactions between the positively charged proteins and the anionic surfactant heads forming the inner micelle wall. At low ionic strength complete solubilization of the protein was observed. A pH higher than the pl of lysozyme and a salt concentration lower than that of the water pool were required for the recovery aqueous phase to ensure the back extraction of lysozyme from the AOT reversed micelles.
Production of 10-deacetylbaccatin III in Taxus cuspidata Suspension Cell Culture
Lee, Gue-Wha ; Kim, Dong-Il ;
KSBB Journal, volume 14, issue 6, 1999, Pages 665~671
In this study, enhanced production of 10-deacetylbaccatin III(10-DAB), a precursor of taxol in semisynthesis, was investigated in Taxus cuspidata cell suspension cultures. The effects of initial inoculum size and sugar concentration were examined to prove the relationship between the production of 10-DAB and cell growth. The cell growth was found to be stimulated in Schenk and Hildebrandt(SH) medium. The lower the inoculum size as well as initial sugar concentration, the faster the cell growth rate. When the initial sugar concentration was dept low, the production of 10-DAB into medium was increased. By using perfusion culture, continuous cell growth was possible until the end of culture and more than 34.67 g/L of cell concentration could by obtained. This is about 2.5 times higher level than that of control batch culture.
The Effect of Oxygen Supply on the Production of Citric Acid from Encapsulated Aspergillus niger
Park, Joong-Kon ; Jeong, Geung-Sik ;
KSBB Journal, volume 14, issue 6, 1999, Pages 672~676
Encapsulated Aspergillus niger was prepared in order to inspect the effect of oxygen supply on the production of citric acid. A. niger cells which had been immobilized in the calcium alginate capsule grew and mycellia penetrated through the capsule membrane after two days of cultivation and covered over all of the capsule after eight days. The mycellia became loose when the nitrogen source was sufficient of oxygen was deficient. The larger amount of encapsulated cells were put into a given growth medium, the smaller quantity of citric acid was produced. The increase of volumetric oxygen transfer coefficient from 1.8
in the flask culture accelerated cell growth rate but did not influence the production of citric acid. The high oxygen supply rate(
) in the concentric air lift reactor hastened the growth of cells and hindered the production of the citric acid. The reduction of nitrogen source level in the growth medium in the concentric air lift reactor increased citric acid production by 40 percent of that of flask cultivation and the culture period was shortened by 3 days. The variation of the geometry of the concentric air lift reactor did not influence the growth rate of encapsulated cells and production rate of citric acid.
Purification of Egg Immunoglobulin IgY
Kim, In-Ho ; Lee, Yong-Tak ; Lee, Chung-Hee ; Chung, Bong-Hyun ;
KSBB Journal, volume 14, issue 6, 1999, Pages 677~681
Purificationi of egg yolk immunoglobulin(IgY) was performed to understand the property of egg immunoglobulin. IgY differs from mammalian IgY in the molecular size(larger), isoelectric point(more acidic), and binding ability with mammalian complement and protein A(nonbinding ability). IgY is also known as
-livetin and exists in egg yolk together with other two water-solubel proteins,
-livetin(chicken serum albumin) and
-glycoprotein) and various lipoproteins(Low density lipoprotein, LDL and High density lipoprotein, HDL) which are the major components of egg yolk. The first step of isolation of IgY is to separate the water-solube proteins from lipoproteins. We report a simple method for separation of water soluble proteins using k-carrageenan and sedimentation. k-carrageenan was found to be effective for removal of yolk lipoprotein as a precipitate. IgY remained supernatant, and was isolated by chromatography on columns of DEAE-Sephacel and G 75 gel filtration chromatography.
Induction of Erythromycin by Virginiamycin Inducing Factor, Virginiae Butanolide C
Kim, Hyun-Soo ; Seong, Lim-Shik ;
KSBB Journal, volume 14, issue 6, 1999, Pages 682~687
Virginiae butanolide C(VB-C) is one of the butyrolactone autoregulators, which triggers the production of virginiamycin in Streptomyces virginiae. In order to investigate the function of VB-C as inducer in other strains, Streptomyces erythraeus was used as a test strain(parent). VB-C binding receptor gene was introduced into S. erythraeus(transformant) and the production of VBs and specific VB-C binding protein were analysed in parent and transformant. When 300ng/ml of the synthetic VB-C was added at 0, 20, 44 h cultivation of the parent and at 44 h cultivation of the transformant, the initial production times a antibiotics were shortened by more than 8 and 6 h, respectively. The transformant showed strong antibiotic activity against B. subtilis. These results suggest that the VB-C might have an ability to induce the production of secondary metabolites in S. erythraeus.
Biodegradation of a Reactive Dye, Remazol Black B in a UASB Reactor
Oh, You-Kwan ; Lee, Sung-Ho ; Kim, Hyo-Seob ; Kim, Yu-Jin ; Lee, Sang-Joon ; Park, Sung-Hoon ;
KSBB Journal, volume 14, issue 6, 1999, Pages 688~695
Biodegradation of the reactive dye, Remazol Black B was investigated in an upflow anaerobic sludge blanket(UASB) reactor. Important parameters studied include dye concentration(20-60 mg/L), glucose concentration as a co-substrate(1,000-3,000 mg/L), hydraulic retention time(3-24 hr), and influent pH(6.0-8.0). Under most conditions tested, the molecules of Black B were degraded readily and completely according to HPLC chromatograms. However, the color removal efficiency based on spectroscopic measurement was always approximately 75%. This suggests that the degradation products have some color intensity corresponding to 25% of the original dye molecules. The maximum influent dye concentration which satisfies the legal discharge limit of color intensity of 400 ADMI was 13 mg/L. and the highest removal rate at this dye concentration was 104 mg/L
Hydrolysis of Castor Oil with Lipases and Organic Solvents
Jeon, Gyu-Jong ; Hur, Byung-Ki ; Yang, Ji-Won ;
KSBB Journal, volume 14, issue 6, 1999, Pages 696~701
The enzymatic hydrolysis of Castor oil for the mass production of ricinoleic acid was studied to find out the optimum conditions such as solvents and the weight ratio of substrate to enzyme. Three different lipases were tested for the hydrolysis of castor oil: lipase from Porcine Pancrease(lipsase PP), lipase from Candida cylindracea(lipase CC), lipase from Candida Rugosa(lipase CR). The poor mass transfer in water caused a low degree of hydrolysis of castor oil. To overcome this problem, organic solvents were used. Among organic solvents tested, hydrophobic solvents gave better results of hydrolysis than hydrophilic solvents. Organic solvents also lowered or changed the effect of pH. Isopropyl ether made complete hydrolysis of castor oil. The ratio of water to isopropyl ether and the ratio of weight ratio of lipase to castor oil were important for the hydrolysis of castor oil. At 30
castor oil was completely hydrolyzed by 4 wt% of lipase in the mixture of isopropyl ether and water(1:1 in volume).
Isolation of an Acid-Labile Gene from the Seaweed Porphyra yezoensis Tissue
Jin, Hyung-Joo ; Park, Sun-Mee ; Kim, Long-Guo ; Jin, Deuk-Hee ; Kong, In-Soo ; Hong, Yong-Ki ;
KSBB Journal, volume 14, issue 6, 1999, Pages 702~706
The genetic responses of aquaculturable seaweed Prophyra yezoensis tissue by acid shock have been compared using differential display technique. The tissue has been challenged in seawater containing 0.05% hydrogen chloride(pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs, respectively. Total RNA was extracted by LiCl-guanidinium method. The cDNA was synthesized by reverse transcription with random hexamers and amplified by PCR with arbitrary primers. The genetic fragment disappeared by acid shock was selectively isolated from agarose gel and sequenced with a DNA auto sequencer. One of the acid-labile gene(605 bp) was identified as a dethiobiotin synthetase gene according to sequence alignment analysis by the NCBI BLAST search program.
Production and Characterization of New Structured-oligosaccharides from Mixed-enzyme of Dextransucrase and
Lee, In-Su ; Kim, Do-Man ; Chang, Pahn-Shick ;
KSBB Journal, volume 14, issue 6, 1999, Pages 707~712
We have produced new-structured oligosaccharides using mixed-enzyme reactor of dextransucrase from Leuconostoc mesenterides B-512FMCM and
-amylase. When the concentrations of sucrose and starch were 10%(w/v) and 5%(w/v), respectively, the maximum yield of oligosaccharides with both dextransucrase(100U) and
-amylase(1000U) was 66.4%. The activity of dextransucrase in mixed-enzyme reactor was increased about 2.5 times by acceptor reaction with starch hydrolyzates. As the activities of dextransucrase:
-amylase were increased from 20U:200U to 500U:5000U, the amount of polymer was increased and the yield of oligosaccharides was decreased. By the addition of sucrose into mixed-enzyme reactor following the prehydrolysis of starch with
-amylase, the yield was increased up to 12% compared with that of mixed-enzyme reactor without the addition of starch hydrolyzate. New structured-oligosaccharides showed heat resistance up to 140
and was stable in acidic condition at pH 3~6.
Properties of Carbohydrase Prepared from Lipomyces starkeyi JLC26
Jun, Sun-Mee ; Kim, Do-Man ; Kim, Do-Won ;
KSBB Journal, volume 14, issue 6, 1999, Pages 713~717
We have isolated a dextranase and amylase constitutive and hyper-producing mutant, Lipomyces starkeyi JLC26, from Lipomyces starkeyi ATCC74054 after mutation using UV irradiation. After partial purification of dextranase and amylase (together DXAMase;both activities were always co-purified) by ammonium sulfate precipitation, CM-Sepharose column chromatography, the specific activities of amylase and dextranase were 5367 and 3045 unit/mg, respectively. The pH effects for activity and stabiligy of both enzymes were similar to each other: Optimum pH and temperature for activity sere at 5.5 and 37
and optimum ranges for stability were at pH 2.5-5.5 and 4-55
, respectively. The reaction end products of dextranase and amylase activities were found to the typical for those of endo-dextranase and endo-amylase. When the carbohydrase and maltotriose were reacted, glucose, maltose, isomaltose, maltotriose, panose and
glucosylmaltotriose were produced by disproportionation reaction.
Isolation and Optimization of Cultivating Conditions of Alkalophilic Strains for Biodegradation of Azo Dye
Kim, Jeong-Mog ; Chung, Hyun-Chae ; Kwon, Oh-Jin ;
KSBB Journal, volume 14, issue 6, 1999, Pages 718~723
In order to treat of alkaline dye-processing wastewater, alkalophilic strains biodegrading azo dye, Acid red 1, is isolated from natural system, and optimal culture conditions are examined using response surface analysis, statistical analysis system program. 15 different species which grow in alkaline culture media are isolated from the effluent and river soil discharged from wastewater treatment plant in dye industrial complex. One strain which has the best decolorization efficiency is chosen, and named as AR-1. The result of the examination of carbon, nitrogen and phosphorus sources which have influence on growth and decolorization reveals that optimum carbon, nitrogen and phosphorus sources are 1.0% fructose, 1.0% polypeptone, 1.0% yeast extract and 0.5%
, respectively. In order to optimize of biodegradation conditions of dye by response surface analysis, the characteristics of decolorization and cell growth according to culture temperature and time are monitered. The result shows that the one is optimum 34.77
for 12.97 hours; the other at 34.73
for 12.96 hours. While, optimal conditions of culture that satisfy both cell growth and decolorization are the temperatures from 32.86
and the period of 10.96 to 15.75 hours, respectively.
Purification of Urokinase and Pro-urokinase and Comparison of their Characteristics
Lee, Seung-Jin ; Byun, Sang-Yo ;
KSBB Journal, volume 14, issue 6, 1999, Pages 724~730
Characteristics and enzyme activity comparison was made between urokinase isolated from urine and pro-urokinase separated from CHO(Chinese Hamster Ovary) cell culture broth. Both of purified urokinase and pro-urokinase resulted 54Kd single band in electrophoresis. Urokinase which was proved as a single molecule by gel filtration showed two separated 33Kd and 21Kd bands by 2-mercaptoethanol reduction. Isoelectric forcusing resulted same pl value of 8.6 for both of them. N-terminal amino acid sequence of urokinase after 159th Ile was Ile-Gly-Gly-Glu-Phe-Thr-Thr-Ile-Glu which was different from another N-terminal sequence of Ser-Asn-Glu-Leu-His-Gln-Val-Pro-Ser-Asn. Thrombolytic activities of both of them were propotional to the enzyme concentration. Urokinase showed thrombolytic activities in an short period of reaction time. Pro-urokinase, however, showed high thrombolytic activity for 2 hours or longer period of reaction time.
Isolation of Dye-degrading Microbes for the Treatment of Dyeing Wastewater and Dye Decoloring
Choi, Kwang-Keun ; Lee, Young-Rak ; Kim, Eui-Yong ; Yoo, Young-Je ; Kim, Sang-Yong ; Lee, Jin-Won ;
KSBB Journal, volume 14, issue 6, 1999, Pages 731~736
87 microbes were isolated from dyeing wastewater collected at Dongducheon and Banweol industrial complex. Five microbes showed excellent ability of color removal and were identified as Shewanella putrefaciens, Aeromonas salmonicida(3 different strains), and Pseudomonas vesicularis. Five identified strains had optimal pH and optimal temperature as 7.0 and 30
for cultivation, and showed morphological characteristics of Gram negative, oxidase negative, rod shape, and non-motility, but their biochemical characteristics were distinguishable. Each single strain of five microbes were tested in the 500 mL flask to treat dyeing wastewater, and achieved about 35% color removal efficiency in average. When two strains were selected and applied to the treatment at same time, color removal efficiency was increased up to 65%. While three or more associations of each strain did not show the improvement of color removal. Inhibition effects by
on the dye degradation were tested and resulted in no effect under 70 ppm concentration.
Biosurfactant Production from Phenanthrene Degrading Bacteria
Han, Chang-Sung ; Yun, Hyun-Shik ; Seo, Hyung-Joon ; Kim, Eun-Ki ;
KSBB Journal, volume 14, issue 6, 1999, Pages 737~741
Phenanthrene degrading bacteria were isolated from the petroleum contaminated soil near an oil tank. Four of 15 strains decreased surface tension of culture broth of phenanthrene-containing minimal media. H6, one of the isolated bacteria decreased surface tension of culture broth below 33 dyne/cm during growth on glucose. H6 was identified as Bacillus subtilis and biosurfactant produced by H6 was lipopeptide. The biosurfactant was produced at 0.13 g/L in the mineral medium containing 2% glucose. Critical micelle concentration(CMC) of the biosurfactant was 52 mg/L. Foaming power was similar to Tween 80 and dispersing power was superior to Tween 80m SDS and Brij30. High thermal stability and emulsion index were also observed.
The Characteristics of Carbon Dioxide Fixation by Chlorella sp. HA-1 in Semi-continuous Operation
Lee, Jae-Young ; Kang, Hyun-Ah ; Yang, Ji-Won ;
KSBB Journal, volume 14, issue 6, 1999, Pages 742~746
The microalgal, Chlorella sp. HA-1, had good
fixation efficiency compared to other algal strains at the same operating condition. In this study, Chorella sp. HA-1 showed similar tolerance both 10% and 20%
concentration. By optimization of the major operation variables such as pH, initial cell concentration, light intensity, the
fixation rate could be raised to a reasonably high value, 372
in a 3 L internally illuminated photobioreactor. In order to maintain the
fixation rate for a long time, the method of semi-continuous operation was employed, in which dilution ratio was the controlling parameter. Starting with the dilution ratio of 0.5 with the increased increment of 0.1, the constant
fixation rate was obtained.
Production and Application of Sophorolipid, A Microbial Surfactant
Cho, Kwi-Joon ; Kim, Young-Bum ; Kim, Eun-Ki ;
KSBB Journal, volume 14, issue 6, 1999, Pages 747~753
Microbial surfactants are more effective and environmentally friendly than many synthetic surfactants. Sophorolipid, a glycolipid type microbial surfactant, is produced from C. bombiocola. Cultivation techniques to increase the productivity have been developed using various carbon sources and reactor setup, reaching its concentration upto 100-300 g/L. Due to its high productivity and non-toxicity, sophorolipid became one of the most promising alternative to synthetic surfactants. Fermentative production of sophorolipid depends primarily on the carbon sources, such as glucose and vegetable oils, and nitrogen sources. Chemical modification of the sophorolipid produces various derivative with different physical properties including hydrophile-liphophilie balance(HLB), emulsion formation, surface tension and dispersing ability. Commercial potentials of sophorolipid in the cosmetic, health care and environment clean-up industries have been discussed.