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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 16, Issue 6 - Dec 2001
Volume 16, Issue 5 - Oct 2001
Volume 16, Issue 4 - Aug 2001
Volume 16, Issue 3 - Jun 2001
Volume 16, Issue 2 - Apr 2001
Volume 16, Issue 1 - Feb 2001
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Molecular Weight Distribution Inside and Outside Capsules Using Coencapsulating Technology
KSBB Journal, volume 16, issue 4, 2001, Pages 321~326
The change of molecular weight inside and outside a capsule produced using coencapsulating technology was investigated. Chitosan and chitosanase were enveloped in this membrane and product released was a loaded the medium by the principle of size exclusion. The leakage of substrate corresponding to the agitation speed was controlled by adjusting the alginate and CaCO
concentrations. The optimal condition of alginate concentration and agitation speed were 0.5% and 40rpm, respectively. Membrane thickness and capsules diameter were 10
m and approx. 3.0 - 1.5 mm, respectively. Molecular weight difference by concentration and alginate viscosity were of little significance. In accordance with the molecular weight distribution versus enzyme concentration relationship, low concentration of enzyme produced high molecular weight oligosaccharides. At a 1.5 mm capsule size the product diffusion rate to outer surface highest. The molecular weight distribution of the released oligosaccharides was ranged from 1000 to 6000 Da. More than 80% of the initial activity of encapsulated enzyme retained after 8hrs of reaction.
Supercritical Fluid Extraction of Catecnin Compounds from Green Tea
KSBB Journal, volume 16, issue 4, 2001, Pages 327~331
Catechin compounds from the Green tea cultivated in Bosung (Chollanamdo) were extracted using supercritical fluid and various additives, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The chromatographic column was packed with LiChrospher 100RP-18(15
m), and water was used as the mobile phase with 0.05% phosphoric acid and acetonitrile. Gradient election was applied to separate EGCG by changing the mobile phase compositions. Comparing the extraction yield of three different types of supercritical fluids, pure CO
, with additives of water and ethanol (5 wt,%), the extraction amount of EGCG was most abundant in the suprcritical CO
with ethanol. However, more was extracted and pure higher purity was achieved by solvent extraction using ethanol.
Removal of NOM Using Biological Rope Media Sedimentation Tank
KSBB Journal, volume 16, issue 4, 2001, Pages 332~336
This paper is intended as an application of the biological rope media sedimentation tank using biodegradability of BAC(Biological activated carbon) to the drinking water treatment system for the removal of NOM. The removal of DOC(Dissolved organic carbon), UV absorbance(UV
254/), and turbidity were evaluated under various operation condition of a biological rope media sedimentation tank such as raw water-media process (Media 1), ozonation-media process (Media 2), and ozonation-coagulation/sedimentation-media process (Media 3). The raw water had DOC concentration of 1.3∼3.4 mg/L, UV
254/ of 0.027∼0.039 cm
-1/, and turbidity of 0.3∼4.5 NTU, respectively. The average DOC concentration were 2.2 mg/L in media 1, 1.8 mg/L in media 2, and 1.3 mg/l in media 3 from raw water, respectively. On the other hand, the DOC concentration in conventional sedimentation tank was 1.5 mg/l. Higher removal of the DOC was noted in media 3 than media 1 and media 2. The UV
254/ of the treated water were 0.037 cm
-1/ in media 1, 0.027 cm
-1/ in media 2, and 0.014 cm
-1/ in media 3 from raw water, respectively The UV
254/ in conventional sedimentation tank was 0.014 cm
-1/ which is similar to that of media 3. Average turbidity of the treated water was 1.1 NTU in media 1, 0.9 NTU in media 2, and 0.5 NTU in media 3, respectively. It is expected that the biological rope media sedimentation tank is a good alternative over the conventional sedimentation process from these results.
Denitrification of Wastewater in a Fluidized Bed Biofilm Reactor
KSBB Journal, volume 16, issue 4, 2001, Pages 337~343
Activated carbon particles of 1.274 mm diameter and sand particles of 0.455 mm diameter were employed as the support of the biofilm formed in fluidized bed biofilm reactors(FBBRs) for the wastewater denitrification. Ethanol was used as the electron donor in the anoxic respiration. The steady-state biofilm thickness increased as the nitrate loading rate increased, and the activated carbon particles induced thicker biofilm than the sand particles. The FBBRs with sand support showed higher efficiency and rate of the nitrate removal than those with activated carbon support, and exhibited the biomass concentration of 37 kg/㎥ and the nitrate removal rate of 21 kg N/㎥d.
Induction of Anthocyanin and Betaine by Salinity Stress in Germinating Seeds
KSBB Journal, volume 16, issue 4, 2001, Pages 344~350
The effect of salinity stress of Brassica olearacea and Capsicum annuum were studied at various levels of salinity conditions(Na-gluconate, K-gluconate, NaCl, KCl). The effects of salinity stress were measured by seedling growth rates and secondary metabolites contents of the stressed plants. Each seedling studied on the response of different salinity stress. Seedling growth of Capsicum annuum was inhibited up to 200 mM salt tolerance and Brassica olearacea was inhibited up to 400 mM salt tolerance. The produced anthocyanin was separated to high value from 200 mM NaCl in case of Brassica olearana and 50 mM K-gluconate in case of Capsicum annuum. The BADH activity was very high in Brassica olearacea seedlings treated with 200 mM NaCl and in Capsicum annuum seedlings treated with 100 mM K-gluconate. The BADH activities were increased during the early culture days, it induced betaine synthesis. The salinity stress promoted BADH activiy, subsequently endogenous betaine contents were increased, and it seemed to be secure seedling from salinity stress. The salinity concentration of 200 mM was effective on the inhibition of seed germination and on the increase of proline accumulation in tissue. The inhibition of seedling growth and accumulation of secondary metabolites in seedling were caused osmotic hypersensitivity against salinity stress.
Measurement of the Organic and Biomass Fractions in Sewage by Respirometry
KSBB Journal, volume 16, issue 4, 2001, Pages 351~355
The information about organic and biomass fractions in sewage is essential for the optimal operation and model calibration of biological treatment processes. In the respect of that oxygen is directly associated with respiration and growth of biomass as well as substrate reduction, the respirometry is well known as a suitable method for the analysis of wastewater composition and active biomass. Thus, the organic and biomass fractions in sewage were measured using respirometry. The fraction of readily biodegradable substrate, slowly biodegradable substrate, inert soluble substrate and inert particular substrate are about 10-16%, 1-8%, 32-50% and 2-47%, respectively. The active heterotrophic biomass fraction is about 10-24%, but the autotrophic biomass was not detected in influent sewage.
Toluene Degradation by Pseudomonas putida in Closed Vial
KSBB Journal, volume 16, issue 4, 2001, Pages 356~359
The ability of Pseudomonas putida to degrade toluene was studied in toluene-containing vials. The strain grows anaerobically in toluene as a sole source of carbon. When the initial toluene concentrations injected in the vial are varied, the changes of headspace toluene concentration and cell density are observed. We set a model for this vial and simulated the vial reactor using Matlab. With a variation of model parameters, simulated results were compared with the experiment.
On the Study of Useful Secondary Metabolites Using Plant Hairy Root Cultures -Effects of Antimicrobial and Disinfectant Activity of Allylisothiocyanate-
KSBB Journal, volume 16, issue 4, 2001, Pages 360~364
It is known as the allylisothiocyanate which is extracted from Wasabia koreana\\\\\\\\`s root. It contains 80% of the oil refining material. The allylisothiocyanate as secondary metabolites of Wasabia koreana plant is a possibility of having the high value which is useful. The research observed the allylisothiocyanate material as the basic study for industrializing to make its mass product. Finally, it shows that the antimicrobial and disinfectant effect against the microbe incubated adding 50 ppm allylisothiocyanate for 15 hours.
Optimization of Culture Conditions for toe Production of Chitinase
KSBB Journal, volume 16, issue 4, 2001, Pages 365~369
Chitinase producing microorganism, Serratia marcescens KY, was isolated from seashore mud around Beobseongpo in Chunnam province by selective enrichment culture. As the colloidal chitin concentration increased, chitinase production was increased. But chitinase production with addition of other carbon sources (glucose, fructose, galactose, maltose, sucrose, starch) was decreased. The effect of nitrogen sources on the chitinase production with serratia marcescens KY was as fellows. The opitimum mineral concentration for chitinase production was K
0.2 g/L and MgSO
0.20 ∼ 0.25 g/L, respectively. The effect of nitrogen sources on chitinase production by Serratia marcescens KY was increased as follows, tryptone > yeast extract > beef extract > asparagine.
Isolation of the Exopolysaccharide Producing Enterobacter sp. and Pnysicochemical Properties of the Polysaccharide Produced by This Strain
KSBB Journal, volume 16, issue 4, 2001, Pages 370~375
For the production of new exo-biopolymers from microorganisms, an exe-biopolymer producing bacterial strain was isolated from the composter used in composting of organic wastes. Bacteriological properties of this strain and physicochemical properties of producing exo-biopolymer were investigated. The isolated strain was identified as Enterobacter sp. through its morphological, cultural and physiological characteristics. The results of color reactions, CPC (cetyl pyridinium chloride) precipitation and infra red absorption spectral analysis indicated that this exo-biopolymer was presumed as an acidic polysaccharide with uronic acid. This polysaccharide was identified as hetero-polysaccharide consisting of galactose, mannose and galacturonic acid by gas chromatography, and the molecular weight of exopolysaccharide purified by gel chromatography were about 370,000 daltons. The polysaccharide solutions(0.50-2.0%, w/v) exhibited non-Newtonian flow behavior with pseudoplastic property and showed the ability of gel formation at above 1.5% (w/v) of polysaccharide concentration.
The Effects of Sucrose and Inoculum Size on the Production of hGM-CSF from Plant Cell Culture
KSBB Journal, volume 16, issue 4, 2001, Pages 376~380
The human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was produced from cell suspension culture of transgenic tobacco which was transformed by using Agrobacterium harboring the hGM-CSF gene. To improve the production of hGM-CSF in batch culture system, the effects of initial sucrose concentration and inoculum size were investigated. The results show that the hGM-CSF production was not affected by small inoculum size in medium containing either low or high concentration of sucrose. However, the production of hGM-CSF was increased under increasing of the inoculum sizes and sucrose concentration. Under the combination of inoculum and sucrose concentration, the maximum hGM-CSF production of 720
g/L was obtained at 90 g/L of initial sucrose concentration and 110 g/L of inoculum size.
Program Development of Integrated Expression Profile Analysis System for DNA Chip Data Analysis
KSBB Journal, volume 16, issue 4, 2001, Pages 381~388
A program for integrated gene expression profile analysis such as hierarchical clustering, K-means, fuzzy c-means, self-organizing map(SOM), principal component analysis(PCA), and singular value decomposition(SVD) was made for DNA chip data anlysis by using Matlab. It also contained the normalization method of gene expression input data. The integrated data anlysis program could be effectively used in DNA chip data analysis and help researchers to get more comprehensive analysis view on gene expression data of their own.
Effect of Sludge Digestion on Removal Efficiency of Heavy Metals from Sewage Sludge Using Thiobacillus thiooxidans MET
KSBB Journal, volume 16, issue 4, 2001, Pages 389~397
The effect of sludge digestion on the leaching efficiency of heavy metals from sludge by bioleaching with Thiobacillus thiooxidans MET was investigated. The used sludges were a non- and anaerobically digested. The leaching efficiency of heavy metals was strongly dependent on the pH of the sludge solution rather than the sludge condition and stolid concentration. The lower the pH the more heavy metal was leached from 3.0 of pH. The sequent orders of leaching heavy metals were Zn, Cu, and Cr. Although the buffering capacity of non-digested sludge was similar to anaerobically digested sludge, the pH decrease rate of the anaerobically digested sludge solution was faster than that of the non-digested sludge solution due to the higher sulfur oxidation rate of T. thiooxidans MET in the anaerobically digested sludge. The amount of leached heavy metals from the anaerobically digested sludge showed higher than that of non-digested sludge at the same pH value. This result might be caused by the difference of the insoluble metal types, which were contained in the sludge. An increase in sludge solids concentration decreased the leaching efficiency of heavy metals in the range of solids concentration 10 g/L to 70 g/L. The optimum ratio of S° to sludge stolid was 0.1 in both the sludge. The bioleaching process of heavy metals with T. thiooxidans MET showed the disinfecting effect over 90% as well as the reduction effect in sludge weight of 20%.
Continuous Production of Agarooligosaccharides Using Packed-Bed Reactor
KSBB Journal, volume 16, issue 4, 2001, Pages 398~402
Enzymatic hydrolysis of agar was carried out continuously to produce agarooligosaccharides by immobilized agarase in Packed-Bed Reactor. The reactor was constructed using a acryl tube with an internal diameter of 10 mm and a useful height of 140 mm. The Packed-Bed Reactor was 11 mL reactor volume as its length : diameter ratio was 14 : 1. The operation condition of reaction was performed with an 1 g/L agar concentration at 40
, 10 mM MOPS buffer(pH 7.0) and with the flow rate 3 mL∼48 mL/h at a dilution rate of 1.09∼5.45 h
-1/. The hydrolysis products was identified DP6, DP4 and DP2 by HPLC. The conversion rate of agar was about 80% and amount of total agarooligosaccharide was 0.88 mg/mL at Packed-Bed Reactor.
A Pilot Study for Microfiltration of Alcohol Stillage Condensate and Permeate Recycle to Fermentation Broth
KSBB Journal, volume 16, issue 4, 2001, Pages 403~408
Distillation condensate generated from downstream processing of microbial alcohol fermentation imposes a serious burden to biological wastewater treatment or anaerobic digestion due to its high contents of SS (suspended solids) and TN (total nitrogen), A pilot scale microfiltration of the stillage condensate with a stainless steel SCEPTER membrane of 0.1
m pore size was carried out to remove SS which was mostly composed of microbial cell residue. A stable permeate flux was achieved when the decanter effluent containing 0.7% of SS was filtered under the conditions of X10 VCR (volume concentration ratio), 2.5 bar of TMP (transmembrane pressure), and 60
. When stillage condensate with 2.6% SS was treated directly with microfiltration, VCR below X3 was recommended for a long duration of filtration. The permeate and retentate obtained from microfiltration were recycled to make-up medium of fermentation. Adding permeate or retentate up to 30% of fermentation volume showed no distinguished undesirable influence during the course of alcohol fermentation. Although only slight improvements in the final amount of CO
evolution and alcohol content were observed, fermentation rate increased so that the required time to reach 450 L/ton of CO
evolution was shortened to 72% of that with normal media.
The Structure Analysis and Biosynthesis of
-glucan by Alcaligenes faecalis
KSBB Journal, volume 16, issue 4, 2001, Pages 409~414
Biosynthesis of curdlan(
-1,3 glucan) was shown by fluorscence on cellufluor medium. The highest production of curdlan was produced when glucose was used as a carbon source and (
was used as a nitrogen source.
-form of curdlan was detected in the fingerprint region (890
) by FT-IR spectrum and shown homogeneous
-1,3 glucan by
NMR spectrum (
-61.2 ppm). Transition of structure from triple helix coil form to random coil form was appeared at 0.1 ∼0.25 M NaOH concentration. It was shown that natural curdlan is a triple helix form in neutral but becomes weak in alkaline condition.
Media Optimization and Comparison of Fermentation Type for Overproduction of Staphylodinase in Bacillus subtilis WB700
KSBB Journal, volume 16, issue 4, 2001, Pages 415~419
To produce staphylokinase (SAK) in B. subtilis WB700, media optimization was carried out and the operation of batch and fed-batch fermentation were compared. Tryptone is a good nitrogen source and its optimum concentration in modified super rich(MSR) media is 15 g/L. When glucose is used as a limiting carbon source in the MSR media, 5 g/L of an optimum glucose concentration was identified for the SAK production under the control of P43 promoter. As the expression of P43 promoter is controlled by the limitation of oxygen, the SAK production was controlled at the 30% DO level in the fed-batch fermentation. Unexpectedly, batch fermentation using MSR media showed 1.5 times higher yield of SAK than that of the fed-batch fermentation. The main cause of the results comes from not achieving higher cell concentration in the fed-batch fermentation and the optimum expression level of P43 promoter under oxygen or nutrient limitations. We could not achieve the increase in cell concentration by any means in batch culture as well as fed-batch culture. The highest yield in the batch culture was 2880 units of SAK activity and 455 mg/L of secreted SAK.
Effects of the Dietary Mixture of Isoflavone on Osteoporosis
KSBB Journal, volume 16, issue 4, 2001, Pages 420~425
Inhibitory effects of isoflavone against osteoporosis were studied with ovaryectomized rats. The diet mixture A of calcium, vitamin D
, and vitamin K
and an additional mixture B with isoflavone were fed for 7 months to ovaryectomized rats. The contents of deoxypyridinoline in the urine of rats fed with the mixture A and B decreased 9.0% and 29.5%, respectively. The trabecular bone areas of proximal tibias of ovaryectomized rats fed with the mixture A and B were measured and compared to the control. They increased 9.9% and 15.8%, respectively, that proved the inhibitory effect of isoflavone against osteoporosis.
Study on the Preparation and Utilization of Sardine Protein
KSBB Journal, volume 16, issue 4, 2001, Pages 426~429
To utilize sardine protein more effectively, fish meat paste products mixing sardine protein concentrate with pollack frozen meat paste at the ratio 0%, 15%, 20% and 25% were produced, and the change of firmness, sensory evaluation and the properties of amino and fatty acid were investigated. The quantity of sardine protein and it was almost gushed out around one hour at 100
. The firmness of the meat paste product was found as 0.54% and was better when the concentrated sardine protein was added at the ratio 15% and it was much higher than just that of pollack meat paste. In that case, total amino acid was the highest as 90.701 mg/g from the point of view of the amino acid composition. In terms of the fatty acid composition, unsaturated fatty acid of raw and boiled sardine was 61,8634% and 61.9384% each. We could find out that the high value of C
20:5/ and C
22:6/ of raw sardine was 7.2931% and 27.7843%, respectively.
Initial Study on Behavior of Microorganisms in livestock Wastewater
KSBB Journal, volume 16, issue 4, 2001, Pages 430~434
Microorganisms of livestock wastewater were microscopically examined to identify bacillus types with optimal absorbance at 630 nm. Growth cuties of microorganisms at various levels of livestock wastewater (5, 10, 20, 30, 40, 50%) and different temperatures (20
) were prepared to determine dilution at which microorganisms did not grow within 1 hour of inoculation, regardless of levels and temperatures. Heat treatment of livestock wastewater at 10 0
for 2 minutes was good enough to inhibit the growth of microorganisms at 20
. A good linear relationship was obtained between levels of livestock wastewater and absorbance at 630 nm (R=98.88%) and dry cell weight (98.98%). The dry cell weight of microorganisms in livestock wastewater was 0.375 g/L.