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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 16, Issue 6 - Dec 2001
Volume 16, Issue 5 - Oct 2001
Volume 16, Issue 4 - Aug 2001
Volume 16, Issue 3 - Jun 2001
Volume 16, Issue 2 - Apr 2001
Volume 16, Issue 1 - Feb 2001
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Prefractionation and Enrichment for the Analysis of Low Aboundance Proteome
KSBB Journal, volume 16, issue 5, 2001, Pages 435~441
In spite of the powerfulness for the simultaneous study of proteome expression and post-translational modification, 2-D PAGE has inevitable limitation on detect low aboundant proteins. Since many of the low abundant proteins are likely to have very important regulatiory functions in cells, separation and analysis of low copy number proteins is an important issue in proteome studies and challenge for 2-D techniques. Among various methods developed to detect low abundant proteins, electrophoretic protein prefractionation, chromatographic protein prefractionation, and subcellular fractionation are explained in this paper. Their practical strengths and weaknesses are also explained with current research trends.
Recovery of Catechin Compound from Korean Green Tea by Solvent Extraction and Partition
KSBB Journal, volume 16, issue 5, 2001, Pages 442~445
Catechin compounds as anticancer and antioxidant were target materials from Korean Green Tea in this work. The methodologies of solvent extraction and partition were utilized to recover catechin compounds from green tea and the optimal experimental conditions were found by comparing the degree of recovery as slovent. extraction times and operating temperatures. The extract was partitioned with chloroform, which was best fit to remove caffeine after the extraction of green tea with 80
water for 40 min. Further, the resulting extract was partitioned in ethyl acetate layer to purify the catechin compounds of EGC, EC EGCG and ECG. This experimental result could be extended to preparative HPLC to obtain EGCG on a commercial scale.
Pretreatment of Used Newspaper to Increase Enzymatic Digestibility
KSBB Journal, volume 16, issue 5, 2001, Pages 446~451
A pretreatment method to increase enzymatic digestibility for waste paper such as newspaper was investigated. Ash content, substrate size and printed ink were considered to be factors that affect on enzymatic hydrolysis. The effect on enzymatic digestibility of varying these factor were measured. Printed ink had the highest effect of the three factors, so a method was developed to remove the ink during pretreatment. Fist, a pretreatment process using a percolation reactor was tried. The digestibility of the substrate pretreated at 170
, however, was less than that of the untreated substrate because only small portion of ink was removed. Therefore, a batch type process at less than 100
was devised. Of several schemes, a method using amonia-hydrogen peroxide mixture on a shaking bath proved most effective. The digestibility obtained from this method was about 85%--approximately 20% greater than the untreated substrate. This proves the pretreatment method was very effective in treating waste paper. The high digestibility obtained from this pretreatment is probably due to the effects of the hydrogen peroxide that can enhance ink removal and substrate swelling.
Control of Ammonium Concentration in Biological Processes Using a Flow Injection Analysis Technique
KSBB Journal, volume 16, issue 5, 2001, Pages 452~458
Concentrations of ammonia in biological processes were controlled by PID controllers and also neural network based controllers (NN controllers). A flow injection analysis system has been to on-line monitor the concentrations of ammonia in a bioreactor. The effect of the analysis error and the residence time of samples on the control performance were studied. The optimal neural network structure was investigated by using computer simulation and found to be a 3(input layer)-2(hidden layer)-1(output layer). The NN controller is often time consuming, but it has advantage over the PID controller in sensitivity. The 3-2-1 NN controller has been applied to control the ammonia concentrations in a simulated bioprocess and also a real cultivation process of yeast. The good control performance showed that the 3-2-1 NN controller based on the FIA system can be used to control the concentration of substrates in biological processes very well.
On-line Monitoring of Glucose and Starch by a Flow Injection Analysis Technique
KSBB Journal, volume 16, issue 5, 2001, Pages 459~465
The on-line monitoring technique for the concentrations fo glucose and starch by FIA(Flow Injection Analysis)system was studied. Glucose oxidase(GOD) and amyloglucosidase(AMG) were immboilized on VA-Fpoxy carrier and integrated into the FLA system. The pH, buffer flow rate and temperature were optimized and the effects of salts and metabolites dissolved in the sample on the activity of immobilized enzyme were investigated. GOD-FIA and AMG/GOD-FIA were applied for the on-line monitoring of the glucose and starch in a simulated bioprocess. The on-line measurements of glucose concentrations by GOD-FIA agreed with off-line data well and the AMG/GOD-FIA with single cartidge system took and advantage over the FIA system with two separated cartridges for the on-line monitoring of starch concentrations.
Characteristics of Sulfur oxidation and the Removal of Hydrogen sulfide by Burkholdera[Pseudomonas] cepacia
KSBB Journal, volume 16, issue 5, 2001, Pages 466~473
A bacterium was isolated from soils in Suwon, Korea for the purpose of H
S removal using a biofilter system. The isolate was gram-negative, rod-shaped, catalase-positive, motile, and the isolated bacterium showed a positve in utilizing energy sources including citrate, mannitol, sucrose, fructors, and trehalsoe. Based on its biochemical characteristics it was identified as Burkholderia(Pseudomonas) cepacia. The growth rate of the bacterium in thiosulfate medium with yeast extract was 0.15 hr
-1/ and generation time was 4.6 hr. The cell productivity was 8.05 mg/L
h and the isolate grew logarithmically up to 12 hr. The maximum rate of sulfur oxidation was 0.18 g-S/L
h. The optimum pH and temperature for the growth of the bacterium were 7.0 and 30
, respectively. The pH range for the growth of B. cepacia was 5.0-8.0. The oxidation rate of thiosulfate was lowered by a substrate thiosulfate when the concentration was higher than 0.12 M. both growth rate and sulfur oxidation rate of Burkholderia(Pseudomonas) cepacia was enhanced about 1.5 times with the addition of 0.2% yeast extract. The removal of hydrogen sulfide was investigated by immobilized B. cepacia with Ca-alginate. The maximum rate removal for H
S was 6.25 g
-1/ when 12 L/h of flow rate was supplied. From this study suggest the immobilized B. cepacia could have a potential for H
-Iysine by Continuous Culture of Corynebacterium glutamicum
KSBB Journal, volume 16, issue 5, 2001, Pages 474~479
Fed-batch culture, single stage and two stage continuous cultures of Corynebacterium glutamicum SH 35 for the production of
-Iysine were performed and compared. In the case of fed batch culture,
-Iysine yield and
-Iysine productivity was 129.2 g/L, 47.0% and 3.08 g/L/h, respectively. In a single-stage continuous culture, optimum dilution rate and pH was 0.1 h
and 6.9, respectively, and optimum concentration of sugar and ammonium sulfate in a medium reservoir was 108 g/L and 25 g/L, respectively. Under the optimized conditions, 67 of cell concentration(
), 44.2 g/L of lysine concentration, 41% of
-Iysine yield and 4.39 g
-Iysine productivity were obtained. In a two-stage continuous culture, optimum dilution rate was 0.075
. Under the conditions, 103 of cell concentration(
) 84.0g/L of
-Iysine concentration and 46% of
-Iysine yield were obtained.
Transformation of Taraxacum mongolicum Hand by Agrobacterium tumefaciens
KSBB Journal, volume 16, issue 5, 2001, Pages 480~485
Genetic transformation in dandelion(Taraxacum mongolicum Hand). was studied. We used for transformation by Agrobacterium tumefaciens strian LBA4404 harboring a binary vector pBI121 carrying the CaMV 35S promoter-GUS gene fusion used as a reporter gene and NOS promoter-NPTII gene as a positive selection marker. To obtain transformed plants, leaf explants of dandelion were cocultured with Agrobacterium tumefaciens LBA4404 for 10 mins, then transferred to MS medium containing 1
M IAA, 1
M BA, 100
g/ML carbenicillin and 50
g/ML kanarmycin sulfate. After two weeks of subculture of the explants, Kanamycin-resistant shoots were formed on explants survived. When subjected to GUS histochemical assay, all of the regenerants showed the GUS-positive responses. Plantlets were be be transformed to soil for further growth.
Antimutagenic Mechanism of Water Extract from Rehmannia glutinosa Liboshitz on 4-nitroquinoline 1-oxide Induced Mutagenesis n E. coli B.r
KSBB Journal, volume 16, issue 5, 2001, Pages 486~492
The antimutagenic mechanism of the fraction III(RG III)separated from the water extract of Rehmannia glutionosa was investigated by Escherichia. coli GW and B/r strains. RG-III treatment did not affect the
-galactosidase activity E. coli GW-1060, 1106, 1107 and 1105. These results indicated that RG-III did not induce RecA protein amplification and did not also prevent the proteolytic cleavage of LexA. The bio-antimutagenicity and survival effect of RG-III on 4-nitroquinoline 1-oxide(4NQO), N-methyl-N-nitor-N\`-nitrosoguanidine(MNING) were investigate by E. coli B/r strains with have different pathway of DNA repai. RG-III slightly increased the survival of 4NQO-treated WP2, WP2s, WP67, CM561, CM611 cells, but the reactivation of survival cannot ve explained by the repair mode. RG-III caused the decrease of mutagenicity and lethality treated with MNNG in ZA159 despite of the increase in WP2, WP2s, WP67, CW561, CM611. Compared with bio-antimutagenic effects of RG-III on 4NQO, greatly increased antimutagenic effects of RG-III were observed with all the E. coli B/r strains tested, but less active in ZA159. These results suggest that RG-III was identified as a blocking agent for preventing the 4NQO induced mutagenesis, and may act as chl-products.
Monitoring of Biological Processes by 2-dimensional Fluorescence Sensor
KSBB Journal, volume 16, issue 5, 2001, Pages 493~499
This work presented the monitoring technique of biological processes by a 2-dimensional fluorescence sensor. The 2-dimensional fluorescence sensor can be used to monitor some important variable during cultivation processes simultaneously. In this study we have monitored fermentation processes of a few microorganisms such as recombinant E.coli, A. terreus and T. vulgaris. and investigated the change of the fluorescence spectra in the fermentation processes qualitatively. The 2-dimensional fluorescence sensor can be also used to monitor biochemical reactions and separation processes and applied for the optimization of biological processes.
Scale-up of Covalently Immobilized Urokinase Column and Repeated Use of It by Solid-Phase Refolding
KSBB Journal, volume 16, issue 5, 2001, Pages 500~504
We scaled up a covalent immobilization system of urokinase to the activated Sepharose and used it repeatedly to cleava a fusion protein consisting of human growth hormone and GST fragment. After scale up from 6 ml to 250 ml. the column system still demonstrated basically the same performance in terms of urokinase immobilization and fusion protein cleavage. When the column was washed with 6 M guanidine HCI after the cleavage reaction, the immobilized urokinase showed no activity probably becasue it was fully unfoled. However, as the denaturant was gradually removed from the column the immobilized urokinase fully regained its bioactivity, which indicated it was properly refolded into is natie conformation as covalently attached to the solid matrix. After 20 cycles of this solid-phase unfolding/refolding. the immobilized urokinase maintained approx. 80% of the initial bioactivity. This method provides and efficient protocol to apply the solid-phase refolding technique to improve the longevity of immobilized enzyme columns.
Controlled Rrelease of Indomethacin using Biodegradable Polymer Microspheres
KSBB Journal, volume 16, issue 5, 2001, Pages 505~510
The preparation, characterization and drug release behaviour of drug(indomethacin) loaded Poly(L-lactic acid)(PLA), tarmarind acetate and levan acetate mircospheres were investigated. Hydrophobic tarmarind acetate and levan acetate were prepared by chemical modification of hydrophilic tarmaried gum and levan and microspheres were made by a solvent evaporation method. In the case of poly(L-lactic acid) microspheres, drug release rate was effected by polymer-drug ratios and drum release was sustained by increasing of polymer content. The yield of microspheres were effected by many factors and the mean size was below 1
m, The IND release profiles from tarmarind acetate and levan acetate micropheres were more slightly less than ploy(L-lactic acid) microspheres.
Development of Two-stage CSTG/TBF System for the Cometabolic Degradation of Gas-phase TCE by Burkholderia cepacia G4
KSBB Journal, volume 16, issue 5, 2001, Pages 511~515
In this paper, we development and operated a two-stage continuous stirred tank reactor (CSTR)/trickling biofilter(TBF)system for the long-term continuous treatment of trichloroethylene (TCE) using Burkholderia cepacia G4. In this reactor system. CDTR with cell recycle from TBF was coupled to the TBF for the reactivation of the cells deactivated during TCE degradation. The critical elimination capacity was determined to be 25.3 mg TCE/L day and the reactor has been stably operated for more than 1 months, which clearly represented that CSTR/TBF system can be used for long-term treatment of TCE.
Determination of Oxygen Transfer Coefficient in Fed-Batch Culture of Streptomyces avermitilis with Concentrated Medium Control
KSBB Journal, volume 16, issue 5, 2001, Pages 516~522
The large-scale production of antibiotics by filamentous mycelial organism requires and adequate supply of dissolved oxygen. In terms of productivity, it means that oxygen transfer is the rate-limiting step. Therefore, the oxygen transfer coefficients(K
L/A) were determined in a broth involving a filamentous mycelial organism such as Streptomyces avermitilis for use in fermentations. To determine (K
L/A) inn a stirred vessel, a great deal of effort is required to provide all the cells with a sufficient oxygen supply. To overcome the oxygen limitation in a batch culture, a fed-batch culture was applied to control the growth rate by an intermittent supply of nutrients. Thus, it was possible to maintain a suitable dissolved oxygen concentration at a low agitation rate. The optimal agitation speed was 350 rpm at low cell concentrations (below 7 g/L) by considering the efficiency of agitation and shear stress. The (K
L/A) was found to decrease from 64.26 to 29.21h.
-1/ when the biomass concentration was increased from 9.82 to 12.06 g/L. In addition, and increase in viscosity was also observed during the growth phase. By comparing the (K
L/A) values for the various agitation and aeration rates, it was found that the effect of an increase in (K
L/A) by aeration was reduced dramatically at high biomass concentrations. However, this effect was not observed when altering the agitation rate. This suggests that controlling the dissolved oxygen concentration by altering the agitation rate was more efficient than increase the aeration rate.
Development of Curdlan Separation Process with Density Gradient Centrfugation
KSBB Journal, volume 16, issue 5, 2001, Pages 523~525
Curdlan is one biopolymer composed of
1,3-glucan and dissolved in a alkali solution but formed salt under neutral or acid condition. It was produced by Agrobactrium species and the separation process is necessary to make pure curdlan from the culture broth. The pH swing separation method was as feasible separation process using solubility changes with the pH difference. however, this method requires a lot of acid and alkali solution also produces a lot of waste. Therefore, an efficient process which could save energy and minimize toxic waste was developed. A density gradient separation process was developed in this research. High density sucrose solution was used as a separation agent. Curdlan was separated from the culture broth when the density of the sucrose solution was 1.15 g/L. Since the curdlan was produced on the surface of cell wall. the pre-treatment of culture broth was necessary. Curdlan recovery yield was increased up to 83% with the homogenization of the culture broth and further increased up to 87% with the treatment of alkai-acid solution.