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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 17, Issue 6 - Dec 2002
Volume 17, Issue 5 - Oct 2002
Volume 17, Issue 4 - Aug 2002
Volume 17, Issue 3 - Jun 2002
Volume 17, Issue 2 - Apr 2002
Volume 17, Issue 1 - Feb 2002
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Engineering Analysis of Biofilter
KSBB Journal, volume 17, issue 2, 2002, Pages 115~120
Biofiltration is a bioprocess treating volatile organic compounds (VOCs) in order to convert the VOCs to harmless products. This review on biofiltration is intended to provide an engineering concept such as removal efficiency, maximum load, elimination capacity and so on. Besides, modeling concept of biofilter is also supplied for designing biofilter system. Quantitative data generated in our research group is shown to explain the engineering concept as well as the modeling idea.
The Principles and Metrical Applications of Immunocsensors
KSBB Journal, volume 17, issue 2, 2002, Pages 121~136
Immunosensors are of great interest because of their potential utility as specific, simple, label-free, direct detection means and provision of reduction in size, cost and time of analysis comparing with conventional immunoassay. In the last two decades, many reports have been published on the use of immunosensors for a wide range of applications to clinical diagnostics, pharmaceutical chemistry, environmental monitoring, biotechnology and food industries. There are also numerous transduction techniques developed such as electrochemical techniques, piezoelectric crystal, and surface plasmon resonance receiving much attention for the direct monitoring of immune reactions at solid surfaces. In this article, the principles, characteristics, structures, fonctions and clinical applications of immunosensors were reviewed
Preparation Of levan Oligosaccharides by Acid Hydrolysis and It Application in Growth of lactic Acid-producing Bacteria
KSBB Journal, volume 17, issue 2, 2002, Pages 137~141
Levan oligosaccoharides and low molecular weight levin were produced from levin by acid hydrolysis and following column chromatography. Levan hydrolysis was progressed proportionally as increased incubation time. In terms of levan hydrolysis reaction, no differences were found from the sources of levan. Optimum hydrolysis conditions for the formation of levan oligosaccharides were, 0.38 M H
; and incubation at 95
for 4 min. The purified products were determined as the mixture of oligosaccharides (degree of polymerization (DP) of 3-6), Two of lactic acid-producing bacteria, Lactobacillus plantarum KCTC 3104 and Pediococcus pentosaceus KCTC 3507, were studied in vitro for their ability to metabolize levin oligosaccharides. Apparently, the growth of both cells were increased by levin oligosaccharide diet, compared with those of levan diets, suggesting that levan oligosaccharides may be beneficial in selectively growth of lactic acid-producing bacteria.
Inhanced Oxygen Supply of Xanthan Fermentations Using either Hydrogen Peroxide or Fluidized Particles in Tower Bioreators
KSBB Journal, volume 17, issue 2, 2002, Pages 142~147
The decomposition of hydrogen peroxide was used for supplementing the oxygen during batch xanthan fermentations in a bubble column bioreactor in order to escape the oxygen transfer limitation that occurred at the high viscosity of culture broths. The xanthan production, however, was inhibited reversibly by dosing hydrogen peroxide. On the other hand, fluidized particles of glass beads with 8 mm diameter led to high gas-liquid oxygen transfer rates in three-phase fluidized beds, which resulted in higher space-time yields of the xanthan production compared to in the bubble column bioreactors.
Extraction of Volatile Essential Oil from Citrus junos Peel by Supercritical Carton Dioxid
KSBB Journal, volume 17, issue 2, 2002, Pages 148~152
The extraction of volatile essential oil using supercritical carbon dioxide with 2%(v/v) ethanol and non-ethanol was performed in a semi-continuous flow extractor at the range of pressures and temperatures 6.9 to 17.2 MPa and 30 to 45
, respectively. When ethanol was added to the co-solvent, the solubility of volatile essential oils was increased up to 90% over the neat CO
value. The most efficiency of extraction of the voilatile essential oils was achieved at 13.8㎫ and 40
by supercritical carbon dioxide with entrainer from Citrus junos peel.
Solid-Phase Refolding of Poly-Lysine fusion Protein of hEGF and Angiogenin
KSBB Journal, volume 17, issue 2, 2002, Pages 153~157
A fusion protein, consisting of a human epidermal growth factor as the recognition domain and human angiogenin as the toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as an inclusion body in recombinant E. coli, yet when the conventional solution-phase refolding process was used the refolding yield was very low due to severe aggregation, probably because of the opposite surface charge resulting from the vastly different pl values of each domain. Accordingly the solid-phase refolding process, which exploits the ionic interactions between a solid matrix and the protein, was tried, however the ionic binding yield was also very low regardless of the resins and pH conditions used. Therefore, to provide a higher affinity toward the solid matrix, six Iysine residues were tagged to the N-terminus of the hEGF domain. When cation exchange resins, such as
Development of Prototype Biosensor for The Detection of Organophosporus Compounds
KSBB Journal, volume 17, issue 2, 2002, Pages 158~161
In this study, a prototype fiber-optic biosensor was fabricated using the inhibition of enzyme reaction by organophosphorus compounds to detect organophosphorus compounds, which is nervous toxic material an? is used as chemical weapon and pesticide. Enzyme, substrate, and inhibitor for enzyme reaction were acetylcholinesterase (key enzyme in nervous cell), acetylthiocholine iodide, and paraoxon (a kind of organophosphorus compounds), respectively. The detection principle of sensor is the detection of enzyme reaction inhibited by organophosphorus compounds by the quantitative measurement of acetic acid, which was achieved by absorbance measurement using litmus solution that maximum absorbance band is changed by pH. To fabricate prototype fiber-optic biosensor, high bright LED and photodiode was used as light source and light intensity detector, respectively. From the experimental results using a prototype biosensor, the linear change of sensor signal was obtained in a range of 0-2 ppm inhibitor concentrations. From these results, it was verified that the quantitative measurement of organophosphorus compounds could be achieved fast (within 2 minutes) and accurately by a prototype fiber-optic biosensor.
Heterologous Expression of Human Ferritin H-chain and L-chain Genes in Saccharomyces cerevisiae
KSBB Journal, volume 17, issue 2, 2002, Pages 162~168
Human ferritin H- and L-chain genes(hfH and hfL) were cloned into the yeast shuttle vector YEp352 with various promoters, and the vectors constructed were used to transform Saccharomyces cerevisiae 2805. Three different promoters fused to hfH and hfL were used: galactokinase 1 (GAL1) promoter, glyceraldehyde-3-phosphate dehydrogenase(GPD) promoter and alcohol dehydrogenase 1(ADH1 ) promoter. SDS-polyacrylamide gel electrophoresis and Western blotting analyses displayed expression of the introduced hfH and hfL. In the production of both ferritin H and L subunits GAL1 promoter was more effective than GPD promoter or ADH1 promoter. Ferritin H and L subunits produced in S. cerevisiae were spontaneously assembled into its holoproteins as proven on native polyacrylamide gels. Both recombinant H and L-chain ferritins were catalytically active in forming iron core. When the cells were cultured in the medium containing 10 mM ferric citrate, the cell-associated concentration of iron was 174.9
Per gram(dry cell weight) for the recombinant yeast YG-L and 148.8
Per gram(dry cell weight) for the recombinant yeast YG-L but was 49.4
Per gram(dry cell weight) in the wild type, indicating that the iron contents of yeast is improved by heterologous expression of human ferritin H-chain or L-chain genes.
Isolation Of Latobacillus Producing Exopolysaccharide and Optimization of its Production
KSBB Journal, volume 17, issue 2, 2002, Pages 169~175
A lactic acid bacterial isolate Lactobacillus ssp. SCU-M which produces exopolysaccharide was identified and its cultural Condition was investigated. The optimum Conditions for exopolysaccharide(EPS) Production Of Lactobacillus ssp. SCU-M were 37
, pH 6.5, using medium composed of 1.5% galactose, 1.0% yeast extract, 0.25% peptone, 0.15% MgSO
, 0.15% K
and 0.1% tween 80 in distilled water. The EPS concentration after 48 hours at the Initial pH 6.5, 37
in a flask culture was 1,680 mg/ℓ.
Solubillzation and Extraction Of Antioxidant Astaxanthin by Micelle Formation from Phaffia rhodozyma Cell Homogenate
KSBB Journal, volume 17, issue 2, 2002, Pages 176~181
-carotene-4-4＇-dione), a natural pigment of pink to red color, is widely distributed in nature particularly in the skin layer of salmonoids and the crust of shrimp, lobster, etc. Recently, it was produced from the yeast culture of Phaffia rhodozyma. Because of its high thermal stability and antioxidant functionality, its applications can be extended into food, cosmetics, and pharmaceutical ingredient beyond the traditional feed additive. Because of its very high lipophilicity, astaxanthin has been extracted traditionally by strong organic solvents such as chloroform, petroleum ether, acetone, etc. In this study, we developed a surfactant-based solubillization system for astaxanthin, and used it to extract astaxanthin from disrupted yeast cells. Among Tween 20, Triton X-100 and SDS, Tween 20 was identified as the most suitable surfactant in terms of extraction capacity and safety. The ethylene oxide group of Tween 20 was identified as the most significant factor to increase the HLB value that determined the extraction capacity. The effects of micelle formation condition, such as the molar ratio of astaxanthin and Tween 20, pH, and ionic strength were also investigated. pH and ionic strength showed no significant effects. The optimal molar ratio between astaxanthin and Tween 20 was 1 : 12. Antioxidant activity of astaxanthin was higher than
-tocopherol. Astaxanthin in the crude extract from the yeast cell was more resistant to air and/or light degradation than pure astaxanthin, probably because of the presence of other carotenoids and lipids.
실관 생물반응기대 고정화된 Enterococcus faecalis RKY1에 의한 숙신산 생산특성
KSBB Journal, volume 17, issue 2, 2002, Pages 182~188
Enterococcus faecalis RKY1 cells were immobilized in an asymmetric hollow fiber bioreactor for application to the continuous production of succinic acid. The media was fed into shell-side of the module using a peristaltic pump, and the products were collected through lumen-side outlet. The number of hollow fibers within the module did not affect the bioreactor efficiency in the transverse operated hollow fiber bioreactor. The steady state at the outlet of hollow fiber bioreactor was reached after 24 hr cultivation at flow rate of 0.25 mL/mim, 12 hr at 0.5 L/min, and 9 hr at 1.0 mL/mm, respectively. The succinate and fumarate concentrations within the hollow fiber bioreactor, however, were as changeful as increasing the flow rate. During continuous operation with the flow rates between 0.5 and 2.0 mL, the productivity of succinate was 8.0-10.9 g/L
hr at 30 g/L fumarate, 4.9-14.9 g/L hr at 50 g/L fumarate, and 7.2-17.1 g/L hr at 80 galL fumarate, respectively.
Continuous Degradation of azo dye by Immobilized laccase
KSBB Journal, volume 17, issue 2, 2002, Pages 189~194
Laccase produced from Trametes sp. was immobilized on CNBr-activated Sepharose-4B (CAS4B) and tested for degradation of azo dyes. Laccase was efficiently immobilized on CAS4B. Immobilization of laccase on CAS4B increased pH, thermal and proteolytic stabilities. Optimum pH and temperature of immobilized laccase were pH 3 and 40
, respectively as same as those of free laccase. The K
mol/ml) values of free and immobilized laccase for Reactive Blue 19 as the substrate were 0.34 and 2.07, respectively V
min) values of them were 0.12 and 0.1, respectively. In repeated batch reactions, conditions retained high stability and degradation of dye for immobilized laccase were pH 5 and 30
. HBT didn\\`t decrease highly activity of immobilized laccase. Immobilized laccase was very stable for degrading dyes continuously in a packed-bed reactor containing laccase immobilized on CAS4B. For continuous degradation of 100
M Reactive Blue 19 and 50
M Acid Red 57 in the presence of 0.1 mM HBT under optimum conditions, immobilized laccase retained 70% of degradation ability even after 30 hours.
Optimization for the Production Factors of Cellulolytic Enzymes of a Fungus, Strain FJ1 by Response Surface Methodology
KSBB Journal, volume 17, issue 2, 2002, Pages 195~202
The production conditions of cellulolytic enzymes by a fungus, strain FJ1, were optimized using response surface analysis. The culture factors which largely affected the production of enzymes such as cultivation time, carbon source concentration, nitrogen source concentration, and composition ratio of carbon sources were employed. Optimizedconditions of the factors above corresponding to each cellulolytic enzyme production were as fellowing: CMCase production was obtained in the conditions of cultivation time of 5.4 days, carbon source concentration of 3.5%, nitrogen source concentration of 0.6%, and composition ratio of carbon sources of 52:48 (avicel:CMC), xylanase appeared in the conditions of 5.3 days, 3.5%, 0.8%, and 54:46, respectively, and
-glucosidase were 7.0 days, 5.0%, 1.0%, and 83:17, respectively, and avicelase were 6.5 days, 4.0%, 0.9%, and 64:36, respectively. The activities of CMCase, xylanase, p-glucosidase, and avicelase predicted by the response surface methodology were 33.5, 52.6, 2.88, and 1.84 U/mL, respectively, and
-glucosidase activity was enhanced up to 74% when compared to that obtained in the experimental conditions.
Effect of Plant Extract(YGF) on Inducing IGF-1 Secretion
KSBB Journal, volume 17, issue 2, 2002, Pages 203~206
The extracts of a lot of (different) plants were studied for inducing the secretion of growth hormone and IGF-1. In the cell test, it was found that the concentration of rat growth hormone was 88 - 99 ng/mL in a certain plant extract treatment group which was at least 6 times higher than the amount in control group. The concentration of IGF-1 in blood was 1,140ng/mL at 8 hours after the oral administration of YGF, which was remarkably much higher than that in control group. This meant that YGF maintained the level of IGF-1 secretion higher in the treated group. In addition, the increase of 6% in bone length was shown for the long-term oral administration of YGF. Thus, YGF seemed to affect significantly for inducing the secretion of IGF-1 which was essential for the physical growth and anti-aging.
Selective Adsorption of a Symmetrie Theophylline Imprinted Membrane Prepare by a Wet Phase Inversion Method
KSBB Journal, volume 17, issue 2, 2002, Pages 207~211
The theophylline imprinted membrane was prepared by a wet phase inversion method. Theophylline was implanted during copolymerization of acrylonitrile with acrylic acid or implanted in the dimethyl sulfoxide solution containing10 wt% copolymer, p(AN-co-AA). Rolling the glass plate, on which the copolymer solution was cast, in water removed the sponge layer and thus made the membrane symmetric. The adsorption selectivity of the membrane toward template molecule was increased with the coagulation temperature of the membrane and the initial concentration of the theophylline and caffeine mixture.