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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 17, Issue 6 - Dec 2002
Volume 17, Issue 5 - Oct 2002
Volume 17, Issue 4 - Aug 2002
Volume 17, Issue 3 - Jun 2002
Volume 17, Issue 2 - Apr 2002
Volume 17, Issue 1 - Feb 2002
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Epoxide Hydrolase-catalyzed Hydrolytic Kinetic Resolution for the Production of Chiral Epoxides
KSBB Journal, volume 17, issue 4, 2002, Pages 321~325
Chiral epoxides are valuable intermediates for the asymmetric synthesis of enantiopure bioactive compounds. Microbial epoxide hydrolases (EHs) are newly discovered enzymes and versatile biocatalysts for the preparation of chiral epoxides by enantioselective hydrolysis of cheap and easily available racemic epoxide substrates. EHs are commercially potential biocatalysts due to their characteristics such as high enantioselectivity, cofactor-independent catalysis, and easy-to-Prepare catalysts. In this Paper, recent progresses in biochemistry and molecular biology of EH and developments of novel reaction systems are reviewed to evaluate the commercial feasibility of EH-catalyzed hydrolytic kinetic resolution for the production of chiral epoxides.
Development of Miniaturized Automatic Chromatography System for validation Study of Chromatographic Resin lifetime
KSBB Journal, volume 17, issue 4, 2002, Pages 326~332
The quality of biopharmaceutical proteins is strongly affected by a manufacturing process employed to produce Et, and thus validation of the manufacturing bioprocess is a very important issue. Chromatography is probably the most widely used bioprocess unit operation for protein purification. In this study, a miniaturized automatic chromatography system was designed and constructed for scale-down studies for process chromatography validation. This system, named MiniValChrom, has the following features: automatic and repeated operation, flexible sequences and intervals among the steps, on-line and real-time monitoring and control, method files savings, etc. Using the MiniValChrom, we peformed a case study of an abbreviated experiment to estimate chromatographic resin lifetime. BSA (bovine serum albumin) and Cibacron Blue 3G-A were used as the model protein and the resin, respectively. The resin deterioration was evaluated by determining and monitoring the HETP and NTP values from the chromatograms every 5 cycles. It was observed that the HETP and the NTP values were changed by 9% after 15 cycles. The resin lifetime validation could be completed by repeating this experiment until the HETP value reached a predetermined value. The MiniValChrom's concept and the protocol suggested in this study can serve as a rapid and economical tool for the validation studies of bioprocess chromatography system.
Isolation and Characteristics of a Homofermentative lactic Acid Bacterium
KSBB Journal, volume 17, issue 4, 2002, Pages 333~338
This study was targeted to isolate and characterize a bacterium producing lactic acid in a large amount. Lactic acid bacteria of about fifty strains were isolated from kimchi, a Korean traditional fermented vegetable food. Strain KH-1 of them was most effective in the lactic acid production and showed 99% homology with Lactobacillus casei from analysis of 16S rRNA sequencing. The conversion ratio of lactic acid from glucose by 1. casei KH-1 was 98% in anaerobic condition, and the lactic acid was composed as racemic mixture of D(-)-and L(+)-lactic acid, 7% and 93%, respectively. This result indicated that L. casei KH-1 was a homofermentative bacterium mainly producing L(+)-lactic acid. The strain KH-1 used glucose as a preferential substrate but not utilized lactose. In investigation of more inexpensive nitrogen source for cultivation of strain KH-1 using industrial MRS medium, when yeast extract and corn steep liquor were used at the ratio of 1 to 1, the molar yield of lactic acid produced per mole of glucose(Yp/s) was 1.09.
Development of a Flow Injection Analysis Technique for On-line Monitoring of Xylitol Concentrations
KSBB Journal, volume 17, issue 4, 2002, Pages 339~344
Flow injection analysis technique for monitoring of xylitol concentrations in biological processes has been developed using xylitol oxidase (XYO) immobilized on VA-Epoxy Biosynth carrier. The immobilized XYO cartridge has been integrated into a FIA system with an oxygen electrode and systematically investigated with regards to the factors which can affect the activity of the immobilized XYO, such as pH, temperature, salt concentration etc. The activity of the immobilized XYO increased with the temperature (
) and sample injection volume (
) and molarity of potassium phosphate buffer (0.1-1 M), but it reached the highest value at pH 8.5. The XYO-FIA system has been also applied for on-line monitoring of xylitol concentrations in a reactor and showed good operational stability and agreement with off-line data measured with HPLC.
Adsorption and Thermostability of Antimicrobial compounds on Water-soluble Silica
KSBB Journal, volume 17, issue 4, 2002, Pages 345~349
The adsorption of the antimicrobial compounds(AMCs) and their heat-resistance were investigated for the packaging film manufacture, wherein, the antimicrobial compounds were adsorbed on a silica component. The naturally source antimicrobial compounds were produced by methylotropic actinomycetes strains MO-16 and MO-17, extracted with ethylacetate. Antimicrobial compounds adsorbed on water-soluble silica had retained activity against Gram(+) and the Gram(-) bacteria after heat treatment at 150
for 5min. The benzoic acid showed strong antimicrobial activity to fungi and was stable to heat treatment. The combination of antimicrobial compound plus benzoic acid was synergistic against test strains. Therefore, we estimated that the water-soluble silica is suitable for the packaging film manufacture as a adsorbent of the antimicrobial compounds.
Antimicrobial Activity and Food Storage of LDPE Silica Film Containing Antimicrodial Compounds
KSBB Journal, volume 17, issue 4, 2002, Pages 350~356
Low density polyethylene(LDPE) film was fabricated with the addition of benzoic acid, or/and JP which contained water-soluble silica adsorbed a natural antimicrobial compounds. The used antimicrobial compounds were isolated from culture broths of methylotropic actinomycetes strains MO-16 and MO-17 which was newly isolated from soils. An antimicrobial compound retained antimicrobial activity after heat treatment at 121
for 5 min. JP, which obtained from Japan as a antimicrobial agent, showed antimicrobial activity in the concentration of 100 mg/mL. The silica LDPE film revealed the growth inhibition of total aerobic bacteria in packaged minced pork compared with commercial film and of E. coil on a contained agar plate. In the storage testing of various packaged foods at room temperature for 30 days, the film showed excellent preservation compared with commercial film in case of small tomato and Agaricus bisporus.
Efficient Extraction Methods and Analysis of Essential Oil from Softwood leaves
KSBB Journal, volume 17, issue 4, 2002, Pages 357~364
For the effecient extraction methods of essential oil, pretreatment of leaves, ratios of water and leaves, extraction time, and collection season from the Chamaecyparis obtusa and Chamaecyparis pisifera leaves were studied. The chemical composition of essential oil was analyzed by GC-MS. The yield of essential oil from ground leaves was higher than that of chopped leaves. The yield of essential oil was not affected much by mixing ratios of water and leaves. The yield of essential oil reached maximum after 5 hours. The content of essential oil of C. obtusa leaves collected during winter was 4.5%, whereas the content of essential oil of C. pisifera collected during fall was 5.3%. The composition of essential oils extracted form C. obrusa and C. pisifera was different. The major constituents in the essential oil of C. obtusa were monoterpene as limonene, terpinene-4-ol,
-selinene, and a-cedrene, and those of C. pisifera was monoterpens as
-pinene, myrcene, limonene, bornyl acetate,
- caryophyllene, longifolene, and
Efficient Purification Of Fused Ferritin[
] using Silica Powder and Gel Filtration Chromatography
KSBB Journal, volume 17, issue 4, 2002, Pages 365~369
An iron-storage protein, ferritin is a spherical shell consisting of 24 H-and L-chain subunits. Soluble form of fused(
chain) ferritin was separated from disrupted recombinant E. coii cells, followed by silica powder adsorption. Ferritin was recovered from silica-poweder by distilled water, which was applied to gel filtration chromatography(GFC). Collected ferritin fractions from the GFC were assayed via iron-uptake and its molecular weight determined using GF-HPLC. Fused ferritin showed a higher activity than the M- or L- chain ferritin by two times.
Cloning and Expression of a Human Homolog of Mouse Gamml, MVGI, Localized in 12q13
KSBB Journal, volume 17, issue 4, 2002, Pages 370~375
Isolation of a gene and determination of its expression pattern are essential in understanding its function. Among the genes localized in 12ql3, stSG3435 EST was chosen to study its expression pattern. The full-length CDNA was cloned by screening of human brain CDNA library and its sequence was determined by serial deletion followed by automated sequencing of the clones with overlapping fragments. The sequence analysis revealed that stSG 3435 CDNA displayed 100% identity to human MYGI and 86% identity to mouse melanocyte proliferation gene-1 (Gamm 1) originally identified from melanocyte, suggesting that MYGI determined by Northern blot analysis revealed the strongest expression in testes with ubiquitous expression in all the tissues tested. In order to investigate the cellular localization of its protein product, the green fluorescence protein gene was fused into the full-length coding sequence of MYGI, Transfection of the fusion construct followed by confocal microscopy resulted in the green fluorescence signal as a punctate state in cytoplasm indication that MYGI was localized in one of the cellular organelles.
-1,3/1,6-glucan by Aureobasidium pullulans SM-2001
KSBB Journal, volume 17, issue 4, 2002, Pages 376~380
Production of the exopolymer by Aureobasidium pullulans SM-2001, UV induced mutant of A. pullulans ATCC 42023, was investigated. The exopolymer produced by A. pullulans SM-2001 was confirmed to be
-1,3-linked homoglucans containing a few
-1,6-linked single glucosyl branches(
-1,3/1,6-glucan) with the nuclear magnetic resonance(NMR) spectrum. The average molecular weight of
-1,3/1,6-glucan produced by A. pullulans SM-2001 was about 2.6
5/ by the gel permeation chromatographic analysis. Sucrose was known to be better carbon source for the production of
-1,3/1,6-glucan than other tested carbon sources in this study. Maximal conversion rate of
-1,3/1,6-glucan was about 50% when the carbon source was 0.5%(w/v) sucrose.
Development of a Microbial Consortium with High Cellulolytic Enzyme Production
KSBB Journal, volume 17, issue 4, 2002, Pages 381~387
A filamentous fungus, strain FB01 showing high
-glucosidase activity was isolated from a compost. This fungus was cocultured with Trichoderma viride to enhance the productivity of
-glucosidase by changing inoculation time of the fungus. The microbial consortium showed higher cellulolytic enzyme production than T. viride alone. The maximal enzyme production was obtained when the microbial consortium was cultured at 30
and pH 6.0 for 10 days with the activities of CMCase,
-glucosidase, and avicelase of 2.0, 0.8, and 0.2 U/mL, respectively. These enzyme activities were 2, 4, and 2 times as high as those of CMCase, p-glucosidase, avicelase from T. viride, respectively, indicating that a synergistic interaction appeared between T. viride and strain FBOI . The serial subcultures with pH control increased
-glucosidase production about 3.2 times. Enzyme production using ricestraw as a carbon source showed that the activities of CMCase,
-glucosidase, and avicelase were 3.69, 0.76, 0.17 U/mL, respectively, and
-glucosidase activity was 1.5 times higher than that of T viride.
The Growth Promotion Effect of Useful Enterobacteria Bifidobacterium aolescentis KCTC 3216 by Combination of Natural Products Bearing Antioxioative Capacity
KSBB Journal, volume 17, issue 4, 2002, Pages 388~395
The growth of enterobacteria, Bifidobacteriurn adolescentis KCTC 3216 was promoted by natural products bearing antioxidative capacity and combined two, three and four kinds of them. B. adolescentis was showed a good growth by Sophorae flos, Phellodendri cofex, Mori cortex radicis, Aurantii nobilis pericarpium, Angelicae gigantis radix, alone, and two mixed combinations were composed of Paeonia japonica and Theae foiium, Epimedii herba and Angelicae gigantis radix, Paeonia japonica and epimedii herba, Atractylodis rhizoma alba and angelicae gigantis radix, and three mixed combinations were oraganized with Theae folium, Paeonia japonica and epimedii herba, Theae folium, Beiamcanda chinensis and Paeonia japonica, Theae foiium, Astragaii radix and Mori cortex radicis, and four mixed combinations were formed with Seiamcanda chinensis, Angelicae gigantis radix, Epimedii herba, Theae folium, and Angeiicae gigantis radix, Epimedii herba, Paeonia japonica, Theae folium, and Epimedii herba, Paeonia japonica, Sophorae flos, Theae folium. The best four mixed combination for the growth of B. adolescentis was mixture of Epimedii herba, Paeonia iaponica, Sophorae flow and Theae foiium, which promoted 2.6 times than that of control, and its antioxidative capacity was also 5.6 times higher, and the ratio of elimination of hydroxyl radical was more than 80% in each dilution rate. As these combinations of natural products will activate some parts of body, they may be applied to pharmaceuitcal applications, functional foods, antiaging tea, also expected to promote useful entero bacterial growth for fermentative beverage bearing multifunction.
Optimization of Biodiesel Production from Waste Frying Oil using Response Surface Method
KSBB Journal, volume 17, issue 4, 2002, Pages 396~402
Biodiesel has attracted considerable attention during the past decade as a biodegradable, nontoxic, and renewable fuel, Several processes for the production of biodiesel have been developed, among which transesterification under alkali-catalysis gives high level yield of methyl esters in short reaction times. In this research, response surface method was applied to optimize the transesterification reaction under alkali-catalysis. It was found that reaction temperature, reaction time, and agitation rate of reactor had profound effects among the seven variables affecting on biodiesel conversion. The optimal temperature, reaction time, and agitation speed were 67
, 68 minutes, and 94 rpm, respectively. Under the optimal conditions, the experimental value of biodiesel conversion was 99.7%.
Effect of YGF251 on Secretion Of IGF-1 in Human Blood
KSBB Journal, volume 17, issue 4, 2002, Pages 403~408
For stimulating the in vivo secretion of IGF-1 (insulin-like growth factor-1) which is well known to promote the various physiological actions in human body, the natural herbal extract, YGF251 (young growth factor 251), was developed and evaluated for its effect as IGF-1 secretagogue in this study. The clinical study was peformed as double blind test, and 31 adult female and male volunteers between the age of 40 and 70 were investigated for their changes of concentration of IGF-1 , insulin level, weight, blood pressure, and liver and kidney functions. As the result of paired sample test on the change of the concentration of IGF-1, in YGF251 treated group, it was 245.6 ng/mL before dosing. The concentration of IGF-1 was increased to 269.3 ng/mL after a month and to 275.6 ng/mL after two months, and both were statistically significant (p〈0.05). While in control group, the concentration of IGF-1 was 280.0 ng/mL before dosing, but decreased to 239.2 ng/mL after a month and to 230.2 ng/mL after two months, and both were also statistically significant (p〈0.05). In YGF251 treated group, the concentration of insulin in blood increased about 2 times after a month dosing as an average level, but in control group, it showed a decrease of 36% compared with before dosing. And there were little changes regarding to the measured weight and blood pressure. Various measured data in order to observe the alteration in liver and kidney functions by the administration of YGF251 showed a little change within measuring error range.
Effect of Plant Extract [FGF271] on Estrogen Replacement
KSBB Journal, volume 17, issue 4, 2002, Pages 409~415
A certain group of phytochemicals such as isoflavone have been proven to act as a phytoestrogen. After thorough the study of different bibliographic herbs excluding soybeans, dates, pomegranates, and other publicized plants, three different edible herbs by Korean Food Regulation were extracted for the animal study on the effect of estrogen replacement. The herbal extract(FGF271) has been orally administered into 51 weeks old partial ovariectomized rats for 5 weeks with the different dosages of 100 and 1,000 mg/kg, respectively. It was observed that 1) serum estrogen level was increased in both 100 and 1,000 mg/kg group, 2) the distension of uterus was made dose dependently and significantly different in 1,000 mg/kg group (p.0.05) from control in the gross findings, 3) the weight of uterus was increased in 1,000 mg/kg group, and 4) the action on reproductive tissues was clear in the microscopic findings in terms of hyperplasia of endometrial epithelial cell, cystic change of submucosa, dilatation of uterus (significantly increased in 1,000 kg/mg), and follicular cystic changes in ovary. As a result, FGF271 seemed to act as a phytoestrogen by inducing the change in ovary and uterus and by increasing the serum estrogen concentration.