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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 17, Issue 6 - Dec 2002
Volume 17, Issue 5 - Oct 2002
Volume 17, Issue 4 - Aug 2002
Volume 17, Issue 3 - Jun 2002
Volume 17, Issue 2 - Apr 2002
Volume 17, Issue 1 - Feb 2002
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Applications of Self Assembled Monolayers(SAMs) for Biosensor
Kim, Ui-Rak ;
KSBB Journal, volume 17, issue 5, 2002, Pages 417~427
Self-assembled monolayers(SAMs) provide a simple route to functionalize electrode surfaces with organic molecules containing free anchor groups, such as thiols, disulfides, amines, silanes and acids. Self-assembled monolayers allows tremendous flexibility with respect to several applicationsdepending upon their terminal functionality or chain length. SAMs of long chain alkane thiols produce a highly packed and ordered surface, which can provide a membrane like microenvironment(cellular) useful for immobilizing biological molecules. The high selectivity of biological molecules(eg. enzymes, DNA, antibodies and antigens) integrated with an electrochemical, optical or piezoelectric transduction mode of analyte recognition offers great promise for their exploitation as efficient and accurate biosensors. Monolayer design plays a key role in controlling the performance of these SAM based biosensors.
Monitoring of Unfolding and Refolding of Immobilized Proteins Using Surface Plasmon Resonance Sensor
Suh, Chang-Woo ; Hwang, Sang-Youn ; Park, Sang-Jung ; Choi, Gang-Sun ; Lee, Eun-Kyu ;
KSBB Journal, volume 17, issue 5, 2002, Pages 428~434
The process monitoring method for the traditional solution-phase refolding could not be directly applied to the solid-phase refolding of immobilized proteins. In this study, we studied the structural change of the covalently immobilized proteins on the gold film of a SPR (surface plasmon resonance) sensor chip by detecting the reflective index change. After confirming the positive relationship between the resonance value and the protein's degree of unfolding, we tested the effect of denaturant concentration such as urea and guanidine-HCL on the protein structure. To investigate the effect of disulfide bonds on the refolded structure, 2 mM dithiothreitol was added during the in-vitro refolding experiment of urokinase (12 disulfides) and disulfide-free myoglobin. Unlike the myoglobin, the RU (resonance unit) value of the urokinase was not fully recovered to the original value, which suggested that the unrecovered RU value represent the miss-shuffled structure. We proposed the unique method to monitor the unfolding/refolding of immobilized proteins by using the simple SPR sensor. This method can be applied to identify the optimal condition for the solid-phase refolding process of immobilized proteins.
Bioleaching of Metal Sulfides using Thiobacillus ferrooxidans 841P
Yang, Ji-Won ; Kim, Sang-Joon ; Park, Ji-Yeon ; Kim, Ho-Jeong ; Lee, You-Jin ;
KSBB Journal, volume 17, issue 5, 2002, Pages 435~439
In this research, Thiobacillus ferrooxidans 841P which has a good activity for leaching of metal sulfide was successfully isolated. From the result of bioleaching using this microorganism, metal ions from ZnS and CuS were highly enriched at 13,000 and 2,300 ppm for 16 and 8 days, respectively. From the result of ferrous iron effect, the selective leaching was certified to be possible by controlling the concentration of ferrous iron. The effect of ferrous iron for the leaching of CuS showed that the leaching capacity of Cu
was proportional to the concentration of ferrous iron while the effect for ZnS was not shown distinctively. When the mixture of ZnS and CuS was introduced into the leaching culture, ZnS was more enriched than single ZnS was. It is because the leached Cu
reacted with less stable ZnS to be precipitated with sulfide from ZnS and ZnS was leached instead of Cu
. From the result for mass metal sulfides, the leaching rate depended largely on the particle size of metal sulfide. Consequently, the effective principle of bioleaching apparatus could be proposed for the enrichment of target metal ion in the mixture ZnS and CuS by this study.
Effects of Wastewater Hydraulic Retention Time and Ammonium Load on Biofilm Formation and Nitrification In Biofilm Airlift Reactor
Lee, Dong-Ik ; Kim, Dong-Jin ;
KSBB Journal, volume 17, issue 5, 2002, Pages 440~445
The effects of hydraulic retention time (HRT) and ammonium load on biofilm formation and nitrification in a biofilm airlift reactor were examined. At low ammonium load (0.15 kg NH
d), more than 95% nitrification efficiency was achieved and the mass fraction of the attached biomass (biofilm) was increased to about 75% as the HRT was reduced to 4.6 h. At high ammonium load (1.9 kg NH
d), the biomass concentration was increased to 3 g/L and about 90% of the total biomass was present as biofilm. Nitrification efficiency was reduced to 80% as the HRT was reduced to 2.8 h at high ammonium load. Reduced HRT and high ammonium load are important factors of early biofilm formation and higher biomass concentration at the start-up of nitrifying wastewater treatment with an airlift biofilm reactor.
Purification of Heavy Chain Ferritin[F
] from Recombinant E. coli
Park , Hyun-Kyu ; Huh, Yun-Suk ; Lee, Jee-Won ; Kim, In-Ho ;
KSBB Journal, volume 17, issue 5, 2002, Pages 446~451
Ferritins are heteropolymers composed of H- and L-chains with a variable ratio. The H-chain ferritin is better for iron uptake, oxidation and physical stability than L-chain ferritin. Therefore, in this study the expression of H-chain ferritin in recombinant E. coli at 37
led to the accumulation of recombinant ferritin. An insoluble form of ferritin was separated from disrupted cells. followed by various primary separation steps with two kinds of butters. Collected samples from the steps were purilied by DEAE-cellulose gels packed in a column. The fractions from the DEAE column were assayed to obtain the amount and the purity of ferritin by using RP- and GF-HPLC.
Cancer Cell Line is Sensitive to Toxins Extracted from Kombucha (fungi), Green Tea and Soybean Milk
Cheun, Byeung-Soo ; Lee, Min-Young ;
KSBB Journal, volume 17, issue 5, 2002, Pages 452~455
In the present study, effect of natural soybean milk and Kombucha green tea on DMEM SiHa cell line. Soybean milks inhibited the proliferation in primary cancer cells in a dose dependent manner. These results show that Kombucha green tea potent inhibits the proliferation of SiHa cancer cells in vitro. Soybeans milk dose dependent inhibited of cellular proliferation in the primary SiHa cell line.
Bioleaching of Metal Sulfides and their Compositions using Thiobacillus ferrooxidans Hetero2
Yang, Ji-Won ; Kim, Sang-Joon ; Park, Ji-Yeon ; Kim, Ho-Jeong ; Lee, You-Jin ;
KSBB Journal, volume 17, issue 5, 2002, Pages 456~460
Thiobacillus ferrooxidans Hetero2 effective for leaching of metal sulfide was isolated. From the results of a single metal sulfide leaching experiment using this microorganism, metal ions from ZnS and CuS were enriched in leachate at 2,350 and 190 ppm for 630 hours, respectively. In term of the ferrous iron effect, ZnS was leached in proportion to the initial Fe
concentration. When a mixture of ZnS and CuS was added to the leaching culture, ZnS was more enriched in leachate at same initial Fe
concentration than ZnS alone. This was because the leached Cu
reacted with the less stable ZnS to be precipitated as sulfide and ZnS was dissolved instead of Cu
. Comparing mixtures of ZnS and PbS, Zn
was less leached than with CuS, because the affinity for PbS as an energy source was larger than CuS. Cu
from CuS was more enriched when coexisting with PbS than with ZnS due to the above relation. From the result of mixtures of metal sulfides leaching experiment, the affinity of T. ferrooxidans Hetero2 for metal sulfide as energy source was ZnS>PbS>CuS. For PbS, the biological leaching capacity was not observed, because of its precipitation with SO
. For iron sulfide, FeS could be leached at 1,600 ppm for 220 hours while FeS
, a very stable metal sulfide, was not leached at all.
Effects of Root Segmentation and Plant Growth Regulator on Decursinol Angelate Production in Angelica gigas Nakai Root Culture
Kim, Ji-Yeon ; Cho, Ji-Suk ; Moon, Ji-Suk ; Kim, Ik-Hwan ; Kim, Dong-Il ;
KSBB Journal, volume 17, issue 5, 2002, Pages 461~466
Decursinol angelate, a new anticancer agent, was produced by root cultures of Angelica gigas Nakai. Difference in specific yields between primary and secondary root was also investigated. It was found that the specific yield of secondary root was higher than that of primary root at various conditions, so that it was through that the stimulation of secondary root formation and growth was desirable. From this point of view, effects of root segmentation on root growth, morphology and decursinol angelate production were examined. Root segmentation increased secondary root emergence and product formation. In addition, the influences of NAA (1-naphthaleneacetic acid) and Tiron (disodium 1,2-dihydroxybenzene-3,5-disulfonate) were studied. NAA enhanced secondary root mass and production of decursinol angelate significantly whereas Tiron showed an inhibitory effect on the action of NAA.
Development of a Row Injection Analysis Technique for Monitoring of ι-lactate in Biological Processes
Lee, Jong-Il ; Kim, Jun-Hong ; Sohn, Ok-Jae ; Kim, Mi-Sun ;
KSBB Journal, volume 17, issue 5, 2002, Pages 467~472
In this study flow injection analysis techniques with immobilized lactate oxidase (LOD) and
-lactate dehydrogenase (LDH) reactors were developed for the monitoring of
-lactate in biological processes. LOD and LDH were immobilized on VA-Epoxy carrier and their reaction characteristics were studied with respect to flow rates (0.8-1.8 mL/min), pH values (pH 4-10), temperatures (25-40
) and NAD
injection volumes (120-280
L) etc. The effects of salts and metabolites dissolved in the sample on the activity of immobilized LOD and LDH were also investigated. The LOD-FIA system were used to monitor the concentration of
-lactate in a simulated bioprocess on-line. The on-line monitoring data agreed with the off-line data measured by HPLC within 6% average error.
A Cell-free Protein Synthesis System using S30 Extract derived from Escherichia coli
Kang, Taek-Jin ; Song, Hui-Kyoung ; Ahn, Jin-Ho ; Choi, Cha-Yong ; Joo, Hyun ;
KSBB Journal, volume 17, issue 5, 2002, Pages 473~476
A cell-free protein synthesis system using S30 extract derived from Escherichia coli A19 and BL21(DE3) was established and optimized for the synthesis of recombinant human erythropoietin. When the condensed S30 extract derived from E. coli A19 was used together with elevated concentration of DNA and amino acids, 5 times higher protein synthesis was achieved compared with normal reaction condition. However, when the condensed S30 extract derived from E. coli BL21(DE3) was used, high yield was not achieved. We mixed each S30 extract with a certain ratio to give comparable yield to S30 derived from A19. A cell-free protein synthesis system established in this study abolishes the need to exogenous addition of purified T7 RNA polymerase, and thus will be of the choise for the simple and economical protein synthesis.
Production and Monitoring of Itaconic Acid from Starchy Materials
Chung, Sang-Yun ; Lee, Jong-Il ;
KSBB Journal, volume 17, issue 5, 2002, Pages 477~483
Itaconic acid was produced during the cultivation of Aspergillus terreus DSMZ 5770 using materials as carbon sources. The optimum C/N/P ratio (1.00 : 0.106 : 0.000228(g/g)) was determined for the growth of A. terreus. A few types of starchy materials were partially hydrolyzed with acids at various pH conditions and employed for the production of itaconic acid. The highest productivity of itaconic acid was obtained when rice starch was pretreated with sulfuric acid at pH 2.5. Using the results of shake flask experiments, A. terreus was used to monitor the bioprocess of A. terreus on-line. 2-D fluorescence intensities at 280/330 nm (ex/em), 475/520, and 545/610 were successfully correlated with the concentration of its phosphate ion and cell mass respectively.
Purification and Some Properties of Lectin from Canavalia ensiformis L.
Roh, Kwang-Soo ; Lee, Dong-Jae ;
KSBB Journal, volume 17, issue 5, 2002, Pages 484~489
Lectins are a cell-agglutinating and carbohydrate-binding proteins widely used in biochemical and immunochemical studies. We have purified the lectin from Canavalia ensiformis L. and investigated its some biochemical properties. The lectin from Canavalia ensiformis L. seed which specifically binds to D-glucose was purified by affinity chromatography using sephadex G-100. The final affinity chromatography step resulted in 637 folds purification with 25% of recovery yield. SDS-PAGE showed double protein band corresponding to 29 and 22 kD. Among the tested red blood cell, the purified lectin agglutinated rabbit red blood cell, but not agglutinated human red blood cells (A, B, AB, O), mouse, bovine, rat and pig. The thermal stability of purified lectin was at 50-70
. The optimal temperature and pH of this lectin were 40
and pH 7.0, respectively.
The Growth Inhibiting Effect of E. coli KCTC 1039 by Combination of Natural Products Bearing Antioxidative Capacity
Kim, Jong-Deog ;
KSBB Journal, volume 17, issue 5, 2002, Pages 490~496
The growth of E. coli was inhibited by the combination of natural products which have antioxidative capacity. E. coli was controlled very effectively by natural products of Scutellaria baicalensis george and Schizandrae fructus, alone, and two mixed combinations were composed of Scutellaria baicalensis george and Schizandrae fructus, Schizandrae fructus and Phellodendri cortex, and three mixed combinations were organized with Scutellaria baicalensis george, Phellodendri cortex and Schizandrae fructus, Scutellaria baicalensis george, Schizandrae fructus and Aurantii nobilis pericarpium, Scutellaria baicalensis george, Phellodendri cortex and Paeonia japonica, and four mixed combinations were formed with Nelumbo nuclfera gaertner, Scutellaria baicalensis george, Paeonia japonica and Theae folium, and Nelumbo nuclfera gaertner, Phellodendri cortex, Paeonia japonica and Theae folium. The growth inhibition ratio of these four mixed combinations was 80% and antioxidative capacities were also 5.7 times higher and the elimination of hydroxyl radical was more than 80% compared with control. As natural products might activate some parts of human body, they could be utilized as pharmaceutical applications, functional foods, antiaging tea, and multifunctional fermentative beverages.
Development of H
S Removal Process with Hybrid Reactor
Son, Hyo-Jin ; Park, Yang-Ho ; Lee, Jung-Heon ;
KSBB Journal, volume 17, issue 5, 2002, Pages 497~502
S gas was introduced into a single reactor, whare cells were mixed with iron solution in the absence of the immobilization particle, the cells were damaged by high concentration of H
S and the H
S removal rate was decreased rapidly. Therefore, a hybrid reactor was developed combining chemical reduction reactor and biological oxidation reactor to remove the toxic effect of H
S on cells and to improve the H
S removal rate. The microbial cells were immobilized on the surface of curdlan particles to enhance Fe(Ⅱ) oxidation rate with repeated fedbatch operation. As a result, iron oxidation rate was increased four times faster than that with free cell Iron solution oxidized with Thiobacillus ferrooxidans in a oxidation reactor was fed into the iron reduction reactor and reduced iron solution was recycled to the iron oxidation reactor. Since iron was precipitate with element sulfur at the high concentration of H
S detected by XRD(X-ray Diffractometer), iron oxidation rate was decreased with reaction time.
On the Development of Functional Health Beverages using Citrus reticulata, Ostrea gigas
Cha, Wol-Suk ; Kim, Chong-Kyun ; Kim, Jong-Soo ;
KSBB Journal, volume 17, issue 5, 2002, Pages 503~507
To development of functional health beverages using Citrus reticulata, Ostrea gigas and medicinal herbs, optimum ratios of Citrus reticulata, Ostrea gigas and medicinal herbs were determined and mixed with several other ingredients. The results of elemental and amino acid analyses with Ostrea gigas and Citrus reticulata were as follows: The mineral contents of Ca and Na in Ostrea gigas were 18.8 ppm and 23.8 ppm, respectively. The content of aspartic acid in Citrus reticulata was 14290.431 pmol, and it contained 8 kinds of essential amino acid. In the sensory test of beverages using Citrus reticulata and Ostrea gigas, the sensory scores(color, taste, flavor) were the best for a mixing ratio of Citrus 1 g, Amomum 0.25 g, Pueraria 0.5 g, Ostrea 12 g, citric acid 1 g, sodium benzoate 0.5 g, aspartic acid 0.5 g and raw sugar 60 g.