Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society for Biotechnology and Bioengineering
Editor in Chief :
Volume & Issues
Volume 19, Issue 6 - Dec 2004
Volume 19, Issue 5 - Oct 2004
Volume 19, Issue 4 - Aug 2004
Volume 19, Issue 3 - Jun 2004
Volume 19, Issue 2 - Apr 2004
Volume 19, Issue 1 - Feb 2004
Selecting the target year
Chlorella as a Functional Biomaterial
KSBB Journal, volume 19, issue 1, 2004, Pages 1~11
Chlorella contains a rich source of biochemical products with applications in the feed, food, nutritional, cosmetic, pharmaceutical and even fuels industries. Chlorella is one of unicellular green algae and is mostly grown in fresh water such as pond and lake. It grows in a manner of nonsexual reproduction so that it multiplies 4~16 times overnight. Large-scale culture is conducted by open pond culture or pure culture using fermenter. Chlorella has various efficacies such as heavy metal removal, degradation of toxic materials, control of arteriosclerosis, immunoprotective effects, anticancer activity and growth-stimulating activity of intestinal bacteria. Chlorella can be used as a taste enhancer and foodstuff, as it has a plenty of essential amino acids, polyunsaturated fatty acids, sterols and chlorella growth factor (CGF). Chlorella is a potential organism which can be utilized for CO
removal and H
Production in environmental area and energy Production.
Biophoton Emission as a Biomarker of Mouse Fatty Liver induced by Carbon Tetrachloride
KSBB Journal, volume 19, issue 1, 2004, Pages 12~16
Recently the measurement of biophoton emission has attracted increasing attention in the study on physiological state of biological systems. We report the measurements of biophoton emission from the mouse fatty liver induced by carbon tetrachloride, CCl
. The hepatotoxin, CCl
in olive oil, was injected intraperitoneally into two groups of ICR mice which were made of 6 mice in each group. The control groups corresponding to the treated groups were prepared with the injections of olive oil only. After the injections, livers of two groups were extracted and measured biophoton emission in 24 hours and 72 hours later, respectively. We also extracted the plasma in the blood and measured the transaminase activity. Results show that biophoton emission from the livers in 24-hour treated group is 69.3
, which is two times more larger than that in 24-hour control group, 29.5
Biophoton emission from the livers in 72-hour treated group is 37.0
. These biophoton results correlate with those of the biochemical assays. We conclude that biophoton emission can be used as a biomarker of mouse fatty liver induced by CCl
Production and Process Monitoring of 5-Aminolevulinic Acid (ALA) by Recombinant E. coli I. Characteristics of ALA Production
KSBB Journal, volume 19, issue 1, 2004, Pages 17~26
In this study the extracellular production of 5-aminolevulinic aicd (ALA) by recombinant E. coli BL2l (DE3) pLysS harboring the plasmid pFLS45 are investigated. Optimum concentrations of succinic acid and glycine for cell growth and ALA production were found to be 30 mM and 15 mM, respectively. Levulinic acid (LA) as an inhibitor of ALAD was added to the culture medium in the end of exponential cell growth phase and its optimum concentration was 30 mM. Growth of recombinant E. coli BL2l (DE3) pLysS (pFLS45) was largely dependent upon the pH value of culture medium. When the pH of culture medium was in the range of 6.0 and 6.5, high cell mass and ALA production were obtained. IPTG induction for the expression of the fusion gene did not enhance the production of ALA. Recombinant cell grew at 30't faster than at 37
, but ALA productivity was lower than at 37
. Repeated addition of glycine, succinic acid, and LA increased the production of ALA and the inhibition of intracellular ALA dehydratase activity, with up to 1.3 g/L ALA having been produced in the cultivation.
Production and Process Monitoring of 5-Aminolevulinic Acid (ALA) by Recombinant E. coli II. process Monitoring by a 2-Dimensional Fluorescence Sensor
KSBB Journal, volume 19, issue 1, 2004, Pages 27~32
2-Dimensional fluorescence sensor has a wide range of excitation and emission wavelengths, that some biogenic fluorphors in a biological process can be monitored simultaneously. The production processes of 5-aminolevulinic aicd (ALA) by recombinant E. coli BL21 (DE3) pLysS harboring plasmid pFLS45 were on-line monitored by a 2-dimensional fluorescence sensor The characteristics of fluorescence spectrum was dependent upon physical and biological factors of a bioprocess such as culture pH, cell mass etc. Some off-line data were correlated to the fluorescence intensity well, which was monitored at some combination of excitation and emission wavelengths by the 2-dimensional fluorescence sensor
Reactive Extraction of Succinic Acid by Amino Extractants and Comparison of Extraction Characteristics of Maleic Acid
KSBB Journal, volume 19, issue 1, 2004, Pages 33~37
Succinic acid is of interested as the raw material of biodegradable polymer. In this study, succinic acid was separated by reactive extraction using amine extractants such as TOA (trioctylamine) and Aliquat 336. The extractability of TOA for succinic acid was higher than that of Aliquat 336. The distribution of succinic acid into organic phase was decreased with increasing pH in aqueous phase. However, the effect of pH on the extractability of Aliquat 336 was little. In the case of maleic acid which has similar structure to succinic acid, the extractability for maleic acid was higher than that for succinic acid. It was mainly due to the difficulty of deprotonation of second carboxylic group by intramolecular hydrogen bonding.
Batch Production of Chiral Epichlorohydrin by Enantioselective Hydrolysis Reaction using Rhodosporidium toruloides
KSBB Journal, volume 19, issue 1, 2004, Pages 38~41
Enantioselective hydrolysis for the producing chiral epichlorohydrin from its racemic substrate was investigated using epoxide hydrolase activity of Rhodosporidium toruloides SJ-4. The effects of reaction parameters including pH, temperature, initial substrate concentration on initial hydrolysis rate and enantioselectivity were analyzed and optimized. The addition of detergent, Tween 20, enhanced the hydrolysis rate and enantioselectivity. Chiral (R)-epichlorohydrin with high optical purity (＞99% ee) and yield of 25% (theoretically 50% maximum yield) was obtained from its racemate of 20 mM.
Molecular Characterization of Epoxide Hydrolase from Aspergillus niger LK using Phylogenetic Analysis
KSBB Journal, volume 19, issue 1, 2004, Pages 42~49
A gene coding for epoxide hydrolase (EH) of Aspergillus niger LK, a fungus possessing the enantioselective hydrolysis activity for racemic epoxides, was characterized by phylogenetic analysis. The deduced protein of A. niger LK epoxide hydrolase shares significant sequence similarity with several bacterial EHs and mammalian microsomal EHs (mEH) and belongs to the a/
hydrolase fold family. EH from A. niger LK had 90.6% identity with 3D crystal structure of lqo7 in Protein Data Bank. Sequence comparison with other source EHs suggested that Asp
374/ and His
374/ constituted the catalytic triad. Based on the multiple sequence comparison of the functional and structural domain sequence, the phylogenetic tree between relevant epoxide hydrolases from various species were reconstructed by using Neighbor-Joining method. Genetic distances were so far as 1.841-2.682 but characteristic oxyanion hole and catalytic triad were highly conserved, which means they have diverged from a common ancestor.
Characterization of different Dioxygenases isolated from Delftia sp. JK-2 capable of degrading Aromatic Compounds, Aniline, Benzoate, and p-Hydroxybenzoate
KSBB Journal, volume 19, issue 1, 2004, Pages 50~56
The aim of this work was to investigate the purification and characterization dixoygenases isolated from Delftia sp. JK-2, which could utilize aniline, benzoate, and p-hydroxybenoate as sole carbon and energy source. Catechol 1,2-dioxygenase (C1, 2O), catechol 2,3-dioxygenase(C2, 3O), and protocatechuate 4,5-dioxygenase(4,5-PCD) were isolated by benzoate, aniline, and p-hydroxybenzoate. In initial experiments, several characteristics of C1 ,2O, C2, 3O, and 4,5-PCD separated with ammonium sulfate precipitation, DEAE-sepharose, and Q-sepharose were investigated. Specific activity of C1 ,2O, C2, 3O, and 4,5-PCD were approximately 3.3 unit/mg, 4.7 unit/mg, and 2.0 unit/mg. C1 ,2O and C2, 3O demonstrated their enzyme activities to other substrates, catechol and 4-methylcatechol. 4,5-PCD showed the specific activity to the only substrate, protocatechuate, but the substrates(e.g., catechol, 3-methylcatechol, 4-methylcatechol, 4-chlorocatechol, 4-nitrocatechol) did not show any specific activities in this work. The optimum temperature of C1, 2O, C2, 3O, and 4,5-PCD were 30
, and the optimal pHs were approximately 8, 8, and 7, respectively. Ag
2+/ showed inhibitory effect on the activity of C1, 2O and C2, 3O, but Ag
3+/ showed inhibitory effect on the activity of 4,5-PCD. Molecular weight of the C1, 2O, C2, 3O, and 4,5-PCD were determined to approximately 60 kDa,35 kDa, and 62 kDa by SDS-PAGE.
Screening of Peroxynitrite and DPPH Raoical Scavenging Activities from Salt Marsh Plants
KSBB Journal, volume 19, issue 1, 2004, Pages 57~61
A peroxynitrite is formed when superoxide and nitric oxide exist at near eqimolar ratio in biological systems. Although not a free radical by chemical nature, peroxynitrite is a powerful oxidant having a wide array of tissue damaging effects ranging from lipid oxidation and inactivation of enzymes and ion channels through protein oxidation and nitration to inhibition of mitochondrial respiration. During our search for new antioxidizing components from natural resources, twenty salt marsh plants were screened for their ONOO and DPPH radical scavenging activities. Among them, methanol extract of Rosa rugosa, lxeris tamagawaensis, Erigeron annus, Tetragonia tetragonoides, Imperata cylindrica, and Suaeda japonica inhibited more than 85% of peroxynitrite produced by 3-morpholinsydnonimine (SIN-1) at a concentration of 5
. In addition, Rosa rugosa, Artemisia capillaris, Erigeron annus and Ixeris tamagawaensis showed significant scavenging effect against DPPH (1,1-diphenyl-2-picrylhydrazyl radical).
A Study on Oxygen Permeability of Polypropylene Membranes and their Temperature Dependency using Medical Oxygen Sensor
KSBB Journal, volume 19, issue 1, 2004, Pages 62~66
The oxygen permeation characteristics of BOPP, OPP and CPP membranes were studied against various thicknesses and temperatures. The experimental results showed that the present method of using electrochemical oxygen sensor was a convenient method for measurement of membrane permeability of oxygen and its activation energy, while the thickness dependency on permeability has an order of BOPP ＞ CPP ＞ OPP. And the activation energy of oxygen permeability showed different values for each membrane ranging from 13.1 kJ/mol to 28.5 kJ/mol, without depending on membrane thickness, presumably due to its depending upon membrane material itself.
Antioxidant Activity of Rosa rugosa
KSBB Journal, volume 19, issue 1, 2004, Pages 67~71
An antioxidant activity of Rosa rugosa extract and its solvent-partitioned fractions was determined not only by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at 37
, using thiobarbituric acid (TBA) but also by evaluating the free radical scavenging effect against DPPH radical, authentic peroxynitrite, and 3-morpholinsydnonimine (SIN-1). All its partitioned fractions including crude extract showed potent scavenging effect against DPPH radical, peroxynitrite, and lipid peroxidation. n-BuOH fraction, in particular, was found to be the most effective in DPPH radical scavenging ability as well as inhibition against lipid peroxidation. The 15% aqueous MeOH fraction also showed a strong potency which was slightly lower than n-BuOH fraction. Based on these results, we suggest that Rosa rugosa could be useful for preventing an oxidative damage.
16S rDNA-PCR and RFLP Analysis for rapid identification of Spoilage Bacteria from low Salt Cucumber Brine
KSBB Journal, volume 19, issue 1, 2004, Pages 72~77
The aim of this study was to isolate and identify the spoilage bacteria in the low salt cucumber brine. The PCR amplicons comprising a portion of the 16S rRNA gene of the isolated colonies were directly sequenced and the untrimmed whole sequencing results of the unknown strains were aligned with the type strains using BLAST of NCBI. Then Sequence Aligner and Sequence Match of RDP confirmed the outcome. The identified isolates were eight species and belong to three genuses: Clostridium, Lactobacillus, and Bacillus. The RFLP pattern of the 16S rRNA gene of isolates verified the identified species. From now on the complex spoiling process of law salt fermented cucumber could be analyzed using the isolated species individually or with certain combinations.
Selection of organic Nitrogen Source and Optimization of Culture Conditions for the Production of Arachidonic Acid from Mortierella alpina
KSBB Journal, volume 19, issue 1, 2004, Pages 78~82
Experiments were carried out to select an organic nitrogen source and optimize the culture conditions for the production of arachidonic acid by Mortierella alpina DSA-12. Corn steep powder(CSP) was selected as an organic nitrogen source based on arachidonic acid production and raw material price. The optimum C/N ratio was in the range of 15 to 17 with the medium containing glucose as carbon source and CSP as nitrogen source. The optimum culture conditions for arachidonic acid production showed 500 rpm agitation and 25
culture temperature at 0.5 vvm aeration. Under the optimum conditions, the concentration of cell, total lipid and arachidonic acid were 21.8 g/L, 10.2 g/L and 3.70 g/L, respectively, from 50 g/L glucose and 18 g/L CSP. In the 500 L fermenter with 0.5 vvm aeration and 200 rpm agitation, the concentration of cell, total lipid and arachidonic acid were 19.8 g/L, 9.1 g/L and 3.67 g/L, respectively, from 50 g/L glucose and 18 g/L CSP. This result showed that an arachidonic acid production could be possible with a bench-scale fermenter using corn steep powder as a nitrogen source.
Effect and Safety of Replacement Therapy for PMS〔post-Premenopausal Syndrome〕
KSBB Journal, volume 19, issue 1, 2004, Pages 83~87
This research was designed to investigate the effects of Estromon including FGF271 (Female Growth Factor 271) which was developed as a phytoestrogen for post- and pre-menopausal syndrome (PMS). The oral administration of two capsules of Estromon twice a day for 3 months significantly improved PMS (Post-/Premenopausal Syndrome) about 5 times more than placebo group (OR=5.04, 95% C.1. 1.40-18.14). In the group of 24 patients having taken Estromon, the concentration of alkaline phosphatase asn the bone marker decreased by -9.3
9.5 IU/L after 3 months with a statistic significance. Since the concentration of osteocalcin as the other bone marker also decreased in more patients in Estromon group than in placebo group, the bone density might be expected to be improved in long-term treatment. Serum human growth hormone level increased in 17 out of 24 patients. Triglycerides decreased by -8.0
40 (mg%) after 1 month and by -4.4
36 (mg%) after 3 months in Estomon group while triglycerides increased in both cases in placebo group (p.0.01). Therefore, PMS patients might benefit from Estromon as a phytoestrogen supplement without any serious side effects.
Preparation and Immunogenicity of the Combined Vaccine Composed of the Polysaccharide Capsule of Samonella typhi and Japanese Encephalitis Virus
KSBB Journal, volume 19, issue 1, 2004, Pages 88~92
The immunogenicity of investigational combined vaccine, composed of the Japanese encephalis virus(JEV) and the polysaccharide capsule(Vi) of Salmonella typhi covalently bound to tetanus toxoid(TT) was evaluated in mice. The mice immunized with combined vaccine elicited higher anti-Vi Immunoglobulin G(IgG) as well as anti-JEV IgG levels than the mice immunized with Vi-TT or JEV alone. The combined vaccine produced four-fold increase in anti-Vi IgG level than Vi-TT alone. In JEV the combined vaccine was significantly more immunogenic than JEV alone and induced six-fold increase in IgG level. Adsorption of combined vaccine onto aluminium hydroxide gel also enhanced IgG level for both Vi and JEV