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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 19, Issue 6 - Dec 2004
Volume 19, Issue 5 - Oct 2004
Volume 19, Issue 4 - Aug 2004
Volume 19, Issue 3 - Jun 2004
Volume 19, Issue 2 - Apr 2004
Volume 19, Issue 1 - Feb 2004
Selecting the target year
Sludge reduction by Enzyme Pretreatment
KSBB Journal, volume 19, issue 2, 2004, Pages 93~97
We investigate the effect of enzyme pretreatment using protease, carbohydrase, and lipase on improvement of sludge treatment efficiency by measuring SCOD and TCOD. The enzyme-pretreatment increases SCOD of excess sludge. In addition, the amount of sludge reduction during digestion, in terms of SCOD and TCOD, are enhanced by enzyme-pretreatment. Among pretense, carbohydrase, and lipase, pretense showed the best enhancement of the sludge treatment efficiency. Sludge digestion followed by ozone and enzyme treatments showed more effective sludge treatment when compared with ozone treatment alone. Therefore, we expect that enzyme pretreatment can be used as a useful tool for enhancing the sludge treatment efficiency.
Study of Hardness Effects of Water in Alcohol Fermentation to Focus Effect of Ozone
KSBB Journal, volume 19, issue 2, 2004, Pages 98~103
The aim of the present study was to investigate how the hardness of groundwater affects in the alcohol fermentation. Ozone plays an important role to enhance the water quality, resulting in 85% reduction of hardness, and 30% increase in total glucose produced due to increased conductivity and biodegradability of water. After all, experiments using ozone are presented for the improvement of alcohol productivity. Although initially increased slightly alcohol production, higher than expected ethanol production was observed, with ozone treatment resulting in 20% higher production.
Comparison of Bacterial Regrowth on Plant- and Coal-based Granular Activated Carbon
KSBB Journal, volume 19, issue 2, 2004, Pages 104~109
Activated carbon has been used in water treatment, because they strongly adsorb organic material including contaminant. Water purifier usually use activated carbon, and bacterial regrowth on that could induce many problems. Model columns, packed with coal- and plant-based granular activated carbon (GAC), were operated with rechlorinated tap water to compare the degree of bacterial regrowth on different GACs. GAC columns decreased the concentration of total organic carbon and chlorine, while they are not good for the decrease of ions. Breakthrough of bacteria were occurred after eight days of operation, and reached 1.1
CFU/mL on coal based GAC and 6.2
CFU/mL on coconut based one. Bacterial activities on GAC were between 15.35 ∼ 29.06
INT-formazan/g-GAC/h. Bacterial concentration and activities were higher in coal based GAC than coconut based one. Bacterial regrowth on GAC was clarified and regrowth effect of coal-based GAC was higher than that of coconut-based one.
Study on herbal extract on the Noni [Morinda citriforia]
KSBB Journal, volume 19, issue 2, 2004, Pages 110~112
The in vitro cell regeneration, the noni extract was mixed into the dilute noni solution and the ability of ROS to degrade noni into H
was measured spectrophotometically. H
was used for producing reactive oxygen species (ROS) in this measurement.
Effect of Ultrasonification Process on Enhancement of Immuno-stimulatory Activity of Ephedra sinica Stapf and Rubus coreanus Miq.
KSBB Journal, volume 19, issue 2, 2004, Pages 113~117
The effect of ultrasonification on immuno-stimulatory activities of the extracts from Ephedra sinica Stapf and Rubus coreanus Miq. was observed by the growth of human immune cells. The extraction yields from water extracts and water extract with ultrasonification at 40
were compared. The highest yield was observed enhancement ratio 2.34% in the extract at 40
with ultrasonification. The growth of human T-cell was increased up to 13.8
in adding 1.0 g/L of the ultrasonification extracts. The treated extracts increased the specific secretion of TNF-
up to 10.5
-4/ pg/cell. It was suggested that of the extracts on water the extraction yield with ultrasonification compared improved the extraction with ultrasonification of low than.
Mechanism of Melanogenesis Inhibition by Melanoston Isolated from Yeast
KSBB Journal, volume 19, issue 2, 2004, Pages 118~124
Melanocytes synthesize melanin within discrete organelle termed melanosomes which are transferred to the surrounding keratinocytes and can be produced in varying sizes, numbers and densities. Skin whitening products have become increasingly popular in the past few years. The most successful natural skin whitening agents are: Arbutin, Vitamin C, Kojic acid, Mulberry, which are all tyrosinase inhibitors. In this work, melanoston, a melanogenesis inhibitor isolated from yeast was studied to understand its mechanism of melanogenesis inhibition. It was found that melanoston was not a tyrosinase inhibitor, while when melanoston was applied to the B16 melanoma cell culture media, the intracellular tyrosinase activity was decreased by more than 30％, When B16 melanoma was stimulated with
-MSH, cell morphololgy was dramatically changed to have lots of dendrites on the cell membrane surface. On the other hand, B16 was treated with
-MSH and melanoston, simultaneously, the change of cell morphology was not so great. This inhibition effect of melanoston was found to be related to the inhibition of intracellular activation and transportation of tyrosinase, which was observed by immunostaining of B16 melanoma using anti-tyrosinase antibody. From these results, melanoston was regarded as an inhibitor to the differentiation of melanoma cells.
Investigation of Antidiabetic Medicinal Plants using an Oriental Medicinal Database
KSBB Journal, volume 19, issue 2, 2004, Pages 125~131
Various medicinal plants were examined as resources for antidiabetic biomaterials, using an oriental medicinal database. The prescription frequency and dosage of medicinal plants in 131 antidiabetic prescriptions were analyzed. Total prescription score of each medical plant was summed up from prescription table and score table. High-scored medicinal plants have been widely studied in the previous research on the diabetic treatment. Consequently, dangui, hwangryun, maekmoondong, ginseng, chick, saengjihwang, omija, bokryung, Jimo and cheonhwaboon were assumed to have high antidiabetic activities.
Biodegradibility Tests of Biodiesel-derived Pentaerythritol Lubricant Oil Bases
KSBB Journal, volume 19, issue 2, 2004, Pages 132~137
Biodegradability test for various synthetic lubricant oil bases derived from biodiesel was carried out. The biodegradability was estimated under aerobic aqueous condition, according to the method by OECD 301 B, which is based on CO
evolution test. The ultimate biodegradability of pentaerythritol methyl esters were estimated as 61.1∼80.3%, at 28 day with which the test compounds were indicated as ultimately biodegradable. Among the tested samples, biodiesel showed the highest biodegradability (83.5%). The validation with several criteria, regarding relative errors of test results, toxicity control and procedure control, was performed through the biodegradability test. The test procedure was validated for all the tested lubricant oil bases and biodiesel, except for petroleum diesel.
The Effects of Extracts from Ginseng, Wormwood and Pine needle in Pulmonary Structure and Anti-oxidant Enzyme in Smoking
KSBB Journal, volume 19, issue 2, 2004, Pages 138~142
Cigarette smoke causes atypical structure of pulmonary (cell structural) and oxidative damage. Therefore, we carried out to determine if exposure to cigarette smoke alters pulmonary structure and anti-oxidant related enzyme in a ICR mice model, when natural product extracts using by manual sprayer. The mice were divided into five groups, control group, sham-treated group (Sham), natural product extracts-treated group (NPE), natural product extracts-treated with smoke-exposed group (NPE-SM) and smoke-exposed (SM) group. All groups are similar to control group in weight, but SM group is lower than the other groups. Microscopic image of the pulmonary structure in SM group showed deleterious alterations in the morphology, but the other groups are maintained in original structure. In anti-oxidant related enzyme, SOD (superoxide dismutase) and catalase, SM group represents the lowest enzyme activity among all groups. These results indicate that the natural product extracts is an efficient tissue protective agent against smoke-induced lung injury.
Biodegradation Characteristics of Dimethyl sulfide [DMS] by Isolated Gordonia sihwaniensis PKL-1
KSBB Journal, volume 19, issue 2, 2004, Pages 143~147
Biodegradation of dimethyl sulfide (DMS) was studied in a batch culture using Gordonia sihwaniensis PKL-1 isolated from a compost biofilter after 100 days of operation for the removal of volatile organic compounds. Optimal pH and temperature for the removal of DMS were 7 and
, respectively. The Michaelis-Menten kinetic constants for DMS removal,
, were 0.0016 mg/(mg-protein)ㆍhr, and 8.05 mg/L, respectively.
Isolation of Lipase Producing Yeast and Optimization of Cultivation Condition
KSBB Journal, volume 19, issue 2, 2004, Pages 148~153
Lipase catalyzes the hydrolysis of glycerides into fatty acids and glycerol. The study of microbial lipases has been stimulated in resent years. It is due to the potential uses of lipases in esterification of oils to glycerol, alcohols and carbohydrates. Development of lipase producing yeast has been focused concerning to the utilization of yeast culture for animal feed. In this study, yeast like cells was isolated from a waste oil and sludge. A strain having higher lipase activity was selected by random mutagenesis using UV-radiation. The optimal cultivation conditions in submerged culture were examined in terms of lipase production. 2.0％ of high fructose syrup, 1,0％ of CSL, and 1.0％ of olive oil were selected as the nutritional media for the production of lipase. The maximum lipase activity of 1.12 U/ml and viable cell number of 8.8
7/ cells/mL were obtained at 27
with an initial pH of 5.0.
Recycled packed-bed reactor for efficient denitrification
KSBB Journal, volume 19, issue 2, 2004, Pages 154~158
Recycled packed-bed reactor emploring immobilized microorganism was suggested in this paper for efficient denitrification. In the batch reactor, the effects of initial oxidation-reduction potential and nitrate concentration on denitrification were investigated. As the initial oxidation-reduction potential was decresed to -70 mV from ＋40 mV, the removal rate of nitrate was increased to 3.33 from 1.25 m9 NO
-/-N/min under the experimental conditions. As the initial nitrate-N concentraion was increased to 200 mg/l, the removal rate of nitrate was proportional to the concentration of nitrate. When the concentration of nitrate-N was 400 mg/min, nitrite was detected, and when the initial nitrate-N concentration was reached at 1,000 mg/l, it took longer time for the complete nitrate removal. In order to decrease the initial oxidation-reduction potential and the nitrate-N concentration in the feed stream, the effluent was recycled to the influent stream in the packed-bed reactor. In the case of recycling, the initial oxidation-reduction potential was decreased to 30 mV from 150 mV, and the initial nitrate concentration could be decreased to 85 from 120 mg NO
-/-N/l. As the result of recycling, the removal rate of nitrate was increased to 91.7% from 49.2%.
-ion effects on the function of
-cyclodextrin Glucanotransferase from B. macerans : An X-ray study
KSBB Journal, volume 19, issue 2, 2004, Pages 159~163
The X-ray structure of the cydodextrin-glucanotransferase of Bacillus macerans was solved by molecular replacement at 2.0
resolution. The refined structure has a crystallographic R-factor of 16.6%, (R
free/ = 20.5%). A new metal binding site occupied by two Ca
2+/-ions was found at an accession channel of the active site. There is a large accumulation of negative charges that bind these Ca
2+/-ions, thereby connecting segment
G (residue 254-276) to the main body of domain A (at
H, residue 283-297). The segment 313-
G contains the catalytic residue Glu258 between subsite 1 and -1 and Tyr260 (subsite 2) which is located at the entrance of the active site. The Ca
2＋/-site 3a,b may have a major role for the activity and specificity of this CGTase, although it is not even conserved for the a-subclass of CGTases.
The Effects of CD-product Specificity upon the Enzyme [CGTase] Reaction Condition
KSBB Journal, volume 19, issue 2, 2004, Pages 164~167
Cyclodextrin glucanotransferase (EC 18.104.22.168, abbreviated as CGTase) is one of the most applied industrial enzymes that produces cyclodextrins from starch and related
-1,4-glucans by intramolecular transglycosylation reaction upon Ca
2＋/ dependent manner. The reaction of CLEC,
-CGTases from Bacillus macerans with the soluble starch as a substrate reveals that the surfactants (SDS, N-octyl-
-D-glucoside) significantly affect not only the overall products of CDs but also their selectivity. The surfactants (SDS, Lubrol PX) trigger the increase of
-CD production, but Triton x-100 and Tween 80 suppress
-CD specificity. Organic solvents (dimethyl sulfoxide, formamide, 2-methyl-2,4-pentandiol, and ethylene glycol) also cause changes of total product and product selectivity.