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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society for Biotechnology and Bioengineering
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Volume & Issues
Volume 19, Issue 6 - Dec 2004
Volume 19, Issue 5 - Oct 2004
Volume 19, Issue 4 - Aug 2004
Volume 19, Issue 3 - Jun 2004
Volume 19, Issue 2 - Apr 2004
Volume 19, Issue 1 - Feb 2004
Selecting the target year
The Prospective of Antigen-presenting Cells in Cancer Immunotherapy
Shim Doo-Hee ; Lee Jae-Hwa ;
KSBB Journal, volume 19, issue 6, 2004, Pages 415~420
All around the world, the rate of attack of cancer diseases has been going up and the number of cancer patients has been increasing every year. Cancer can be divided into malignant tumor and benign tumor according to its growth appearance. Many studies and experiments have been conducted and the various treatment are being created to find the way to care malignant. Dendritic cells (DCs), which is an agent of cancer treatments by using an immune reaction in our body, plays an important role to present by a tumor antigen to cytotoxic T-cell and help them to attack the tumor cell directly. However there are some defects of this therapy. Soluble human leukocyte antigen-immunoglobulin fusion protein (HLA-Ig) based artificial antigen presenting cell (aAPC) as the antigen presenting cell (APC) which is complement and overcome some of the limitations of dendritic cell-based vaccines and ex vivo expansion of human T cells is new method for cancer therapy. In this article, we are reviewing the role of DCs and the treatment with it, and searching for the possibility of the new development of immunotherapy for cancer.
Comparison of Extraction Methods for Aglycone isoflavones from Korean Soybean
Lee Kwang Jin ; Row Kyung Ho ;
KSBB Journal, volume 19, issue 6, 2004, Pages 421~426
The extraction and separation of isoflavones from Korean soybean were peformed by various mechanical and chemical extraction methods. They included solvent extraction, stirring, supersonification and sub/supercritical water extraction. From the experimental results of the variation of solvent extraction by change in composition, the increase in extraction of a specific compound by stirring or supersonic energy, and the application of supercritical fluid with superior solvating power over solvents, the sonification was the most desirable extraction method in extracting aglycone isoflavones, daidzein and genistein from Korean soybean.
Extraction of Glabridin from Licorice Using Supercritical Carbon Dioxide
Cho Yun-Kyoung ; Kim Hyun-Seok ; Kim Ju-Won ; Lee Sang-Yun ; Kim Woo-Sik ; Ryu Jong-Hoon ; Lim Gio-Bin ;
KSBB Journal, volume 19, issue 6, 2004, Pages 427~432
The purpose of this study is to investigate the feasibility of a cosolvent-modified supercritical
extraction technique for the production of licorice extracts with high levels of glabridin. The effects of various parameters such as the type and amount of modifiers, extraction temperature (
) and pressure (
) on the extraction efficiency were examined at a fixed flow rate of 1 mL/min. The organic solvent extraction with pure methanol was also conducted for a quantitative comparison with the
extraction. The recovery of glabridin from licorice was found to be extremely small for pure
. However, the addition of modifiers such as ethanol and acetone to
resulted in a significant improvement in the recovery of glabridin. The recovery of glabridin was observed to increase with pressure at a constant temperature. Furthermore, the purity of the glabridin obtained from the
extraction was higher compared with the organic solvent extraction.
Effects of Red Ginseng Total Saponin on the Polycystic Ovaries in Rats
Lee Jiae ; Lim Sung-Chul ; Lyu Ae-Ran ; Bae Jin-Gyu ; Kang Seong-Soo ; Kim Jong-Choon ; Kim Sung-Ho ; Kim Jeong-Wook ; Choi Bum-Chae ; Bae Chun-Sik ;
KSBB Journal, volume 19, issue 6, 2004, Pages 433~436
Polycystic ovarian syndrome (PCOS) is a very common endocrine disorder in women of reproductive age. There are some evidences that nerve growth factor (NGF) is involved in the pathogenesis of PCOS. In this study, we investigated the effect of Korean red ginseng total saponin (GTS) on the ovarian morphology and NGF expressions in the ovaries, pituitary and hippocampus. The oil control animals were injected with 0.2 ml oil/rat. Animals in estradiol valerate (EV) control group were injected i.m. with 4 mg EV in 0.2 ml oil/rat. The GTS was administered (100 mg/kg) i.p. every other day for 60 days, beginning 1 day after the EV injection. PCO was induced by a single injection of EV (4 mg, i.m.). At day 60, the expressions of NGF were examined by immunohistochemistry. The main findings were as follows; PCO was fully developed with a single i.m. injection of EV, and PCO showed the increased expression of NGF, and GTS administration decreased NGF expressions in the ovaries without affecting pituitary and hippocampus significantly. The present results demonstrate that GTS attenuates PCOS by the stimulation of NGF expression.
Inhibitory Effect of Gardenia Fruit Extracts on Tyrosinase Activity and Melanogenesis
Kwak Jung-Hoon ; Kim Yong-Hae ; Chang Hae-Ryong ; Park Chul-Woo ; Han Yeong-Hwan ;
KSBB Journal, volume 19, issue 6, 2004, Pages 437~440
To determine the inhibition of tyrosinase activity and melanogenesis, gardenia fruit was extracted initially with
ethanol (w/v), The ethanol extract was fractionated subsequently with hexane, chloroform and ethyl acetate in that order. The inhibitory effect of the ethanol extract on tyrosinase activity was higher than water extract. When 10 mg/mL of ethyl acetate fraction was applied, the inhibition ratio of tyrosinase activity was much higher (
) than that of arbutin. The inhibition of melanogenesis using B16F10 melanoma cell, the ethanol extract also showed higher inhibitory effect than water extract. The highest inhibitory activity of melanogenesis was also shown in ethyl acetate fraction (
at the cone. of
). These results suggests that gardenia extracts might be used to be a potential agents for whitening
Cellular Iron Uptake from Aqueous Solutions depending on Reaction Conditions by genetically engineered Saccharomyces cerevisiae
Kim Sang-Jun ; Chang Yu-Jung ; Park Chung-Ung ; Jeong Yong-Seob ; Kim Kyung-Suk ;
KSBB Journal, volume 19, issue 6, 2004, Pages 441~445
Cellular iron uptake was performed in the yeast Saccharomyces cerevisiae that transformed with human ferritin H- and L-chain genes. The recombinant yeasts were enriched in YEP medium supplemented with
galactose for 3 days and the iron uptake was followed by incubating the cells with iron in 20 mM MOPS buffer (pH 6.5). The reactions were examined under different conditions including the iron compounds of Fe(II) and Fe(III), the concentration of iron, the concentration of cells and the reaction time. From our results, the recombinant yeast YGH2 producing H-chain ferritin showed higher cellular iron concentration at the cell concentration of 100 mg/ml than 200 mg/ml. Iron presented as Fe(II) rather than Fe(III) was taken up more efficiently. Iron uptake increased slightly when iron was added up to 14.3 mM Fe(II) and then its cellular iron concentration was
cell wet wt. In addition, the iron uptake reaction reached to maximum at about 2 hr incubation.
Culture Conditions for Hydrogen Production of Enterobacter cloacae YJ-1
Lee Ki-Seok ; Kang Chang-Min ; Chung Seon-Yong ;
KSBB Journal, volume 19, issue 6, 2004, Pages 446~450
We investigated the effective culture conditions of anaerobic bacteria, Enterobacter cloacae YJ-1 on hydrogen production. It was cultured with 60 mL of working volume at
, 120 rpm for 40 h. With culture time, hydrogen production and cell growth increased, but residual glucose and pH decreased. When the
of glucose was used as single carbon source, hydrogen production was 975.1 mL/L. To enhance hydrogen productivity, mixed carbon sources of glucose and sucrose were added. The maximum hydrogen production was earned at the mixing ratio of 25:75, and it was 1319.5 mL/L. When we added 50 mM of phosphate to protect the pH drop in culture broth, hydrogen production increased 1.3 times more than that of initial concentration. The organic nitrogen sources were more effective than inorganic nitrogen for hydrogen production. Among organic nitrogen, yeast extract was the most effective and its hydrogen production was 1691.3 mL/L. Among 9 of mineral sources, Ferric citrate and
were especially effective, and their productions were 1782.3 mL/L and 1784.8 mL/L, respectively.
Medium Composition Affecting Production of Bacterial Cellulose by Gluconacetobacter hansenii PJK in an Agitated Culture
Jung Jae Yong ; Chang Ho Nam ; Park Joong Kon ;
KSBB Journal, volume 19, issue 6, 2004, Pages 451~456
The effects of variation in composition of the medium on the conversion of Gluconacetobacter hanseii PJK cells producing cellulose (
) to non-cellulose producing (
) mutants and the production of bacterial cellulose (BC) in an agitated culture were investigated. The impeller speed greater than 500 rpm was required to decrease the population of
mutants to minimum in a basal medium containing
ethanol because the optimum impeller speed to minimize the population of
mutants increased with the concentration of ethanol added to a basal medium. Ethanol fed-batch culture could not increase the BC production in an agitated culture unlike that of a shaking culture. The amount of BC produced in a basal medium containing
more than that of the same medium with
. Increase in the concentration of acetic acid in a basal medium decreased the BC production. The pH control of the culture broth increased the cell mass in the batch culture and improved the production yield of water-soluble polysaccharide (WSPS), but did not affect the production of BC.
Production of Mycelium and Expolysaccharides by Fed-batch Culture of Agaricus blazei
Kim Hyun Han ; Na Jeong-Geol ; Chang Yong Keun ; Lee Sang Jong ;
KSBB Journal, volume 19, issue 6, 2004, Pages 457~461
DO-stat fed-batch cultures of Agaricus blazei were carried out using various feeding solutions, for the production of mycelial biomass and exopolysaccharides (EPS). It was observed to be more effective to use a feeding solution containing both carbon and nitrogen sources than that containing only carbon source. The best result was obtained when a feeding solution containing 450 g/l glucose, 60 g/l yeast extract, 30 g/l soytone peptone was used. The maxium mycelial biomass and EPS concentrations were 36.5 g/l and 10.9 g/l, respectively, at 100 hours of cultivation. The mycelial and EPS productivities were 0.37 g/l-h and 0.11 g/l-h, respectively. As compared with the batch culture, the mycelial biomass concentration and its productivity were 6.0- and 2.2-folds increased, respectively. The EPS concentration and its productivity were increased by 4.7 times and 1.8 times, respectively.
Cloning and Expression of Inositol Monophosphatase Gene from Streptomyces coelicolor A2
Kim Jin Kwon ; Choi Hack Sun ; Kim Seong-Jun ; Kim Si Wouk ;
KSBB Journal, volume 19, issue 6, 2004, Pages 462~466
Mycothiol (MSH), a low molecular antioxidant thiol compound, was purified and analyzed from Streptomyns coelicolor A2 by the monobromobimane fluorescence detection method modified by this lab. Through HPLC chromatpgram, MSH fraction was obtained following the elution time of standard MSH (donated by Dr. Robert C. Fahey). That MSH showed the highest concentration among the thiol compounds contained in the cell indicated that MSH was the key thiol compound having antioxidant activity. To understand the role of gene of inositol monophosphatase (I-1-Pase) involved in the MSH biosynthesis, it was isolated from S. coelicolor A(3)2 and cloned and overexpressed in the Escherichia coli. The expressed I-1-Pase was purified through Ni-NTA column. The soluble protein consisted of 281 amino acids, and the molecular weight was 32 kDa. I-1-Pase of S. coelicolor A(3)2 had the sequence homology with those of human and E. coli by 24 and
, respectively, and had two conserved domains (mofif A and motif B) which were typical of I-1-Pase.
Regeneration of ATP through an Activated Glycolytic Pathway in a Cell-free Extract and its Application for Protein Expression
Kim Dong Myung ; Keum Jeong Won ; Kim Tae Wan ; Oh In Seok ; Choi Cha-yong ;
KSBB Journal, volume 19, issue 6, 2004, Pages 467~470
We have investigated the key parameters affecting ATP regeneration in a cell-free protein synthesis system derived from Escherichia coli. When glucose-6-phosphate was used as an energy source, the efficiency of ATP regeneration sharply responded to pH change of reaction mixture. In addition, both productivity and reproducibility of protein synthesis was substantially enhanced by introducing appropriate amount of NAD into the reaction mixture. As a result, through the activation of glycolytic pathway under an optimal pH, the batch cell-free system produced over
of protein in a 1 mL reaction.
Enhanced Production of Oleanolic Acid by the Elicitation in Oldenlandia diffusa Suspension Cell Cultures
Lee Yong-Il ; Kim Dong-Il ;
KSBB Journal, volume 19, issue 6, 2004, Pages 471~477
Oldenlandia diffusa is a Chinese medicinal herb with antitumor activity capable of suppressing the growth of some cancer cell lines. Oleanolic acid and ursolic acid are triterpenoid compounds that exist in Oldenlandia diffusa. Recently, these have been noted for anti-inflammatory, anti-cancer, and hepato-protective effects. Application of both plant growth regulators, 2,4-D and kinetin, was found to be essential for the initiation of callus and suspension cells. Leaf blades of Oldenlandia diffusa was transformed into callus on Schenk and Hildebrandt medium supplemented with 0.5 mg/L 2,4-D and 0.1 mg/L kinetin, while optimum initiation condition for suspension cells of Oldenlandia diffusa was determined to be 0.75 mg/L 2,4-D and 0.1 mg/L kinetin. Chromatographic separation of oleanolic acid from its derivatives was achieved using Rexchrom S5-100-ODS column. Analytical conditions for oleanolic acid were determined as follows: flow rate at 1.0 mL/min, UV length at 200 nm and mobile phase of
water. Production of secondary metabolites was found to be increased by the treatment with elicitors or signal transducers. The maximum production of oleanolic acid was 99.6 mg/L in cultures with 0.5 mM salicylic acid. It is 1.74 times higher than that of control.
Production of Organic Acids from Food By-Products - Fermentation Characteristics of Propionibacterium acidipropionici -
Jin Sun-Ja ; Ju Yun-Sang ; Hwang Pil-Gi ; Choi Chul-Ho ; Lee Eui-Sang ;
KSBB Journal, volume 19, issue 6, 2004, Pages 478~483
The optimum fermentation conditions of Propionibacterium acidipropionici were determined for the maximum production of organic acids. Three strains of P. acidipropionici, ATCC 25562, 4875, 4965 were selected to test the productivity of organic acids in the batch fermentation. Nutrients and environmental conditions on cell growth were defined by series of experiments, and the optimum amounts of peptone, yeast extract were determined to be
(w/v), respectively. The yields and productivity were highest at pH 6 among the ranges of
, and ATCC 4965 was determined to be the best strain compared to the others by getting total productivity of 0.29 g total acids/L/h. Approximately
moles of propionic acid and
moles of acetic acid were produced from 1.5 moles of glucose.
Production of Organic Acids from Food By-Products - Mass Production of Organic Acids by Continuous Flow Ceil Recycling Fermentation -
Ju Yun-Sang ; Jin Sun-Ja ; Hwang Pil-Gi ; Choi Chul-Ho ; Lee Eui-Sang ;
KSBB Journal, volume 19, issue 6, 2004, Pages 484~488
Fermentation studies were conducted in batch and continuous flow cell-recycle reactors with food by-products as substrates. The genus Propionibacterium acidipropionici ATCC 4965 was utilized in the production of organic acids. Good performance was achieved in the batch fermentation using hydrol as a carbon source and corn steep liquor (CSL) as nitrogen and vitamin sources. Product yields and productivity based on maximum values were 0.80 g total acids/g glucose and 0.26 g total acids/L/h, respectively, when
, (w/v) of hydrol and
, (w/v) of CSL were utilized. Continuous fermentation with cell-recycling system using the optimum amounts of substrates resulted in dramatic increase in cell concentration (X) and maximum productivity (P). Compared to the batch fermentation, X and P were increased by as much as 21 and 13 times, respectively, at the dilution ratio of
, indicating that cell recycling fermentation of food by-products provides valuable means for the mass production of organic acids as well as utilizing cell mass as good nutrient resources.
Investigation of Acyl Chain Specificity of Lipase-OF 360,000 on the Hydrolysis of Fish Oil
Park Ji-suk ; Kim Han-Ok ; Kho Hye-won ; Hur Byung-Ki ;
KSBB Journal, volume 19, issue 6, 2004, Pages 489~493
The hydrolysis characteristics of various fatty acids composing the fish oil was investigated for function of acyl chain specificities using Lipase-OF 360,000 from Candida cylindracea. The hydrolysis of fatty acids decreased with the increase of the number of carbon and double bond in the fatty acids, in case that the number of double bond and the position of the first double bond from the methyl group of fatty acids were the same. The position of the first double bond was found to be an acyl chain specificity of Lipase-OF 360,000 for the hydrolysis of fish oil. Lipase-OF 360,000 also showed the another acyl chain specificity that the increase of double bond of fatty acids, having the same number of carbon and the position of double bond, brought about the decrease of hydrolysis.